FastQCFastQC Report
Wed 25 May 2016
SRR490961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49127668
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656830.13369859118898134No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT498060.10138075350940737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG216800.033.757612
ATCTCGA299800.030.362681
TCTCGAG351150.025.8081382
ATATCTC322000.023.3580281
TATGGAT361400.022.9171242
GATCCGG601000.022.593661
CTCGAGG424300.021.2580763
ATATGGA400850.021.088211
ATCCGGC655200.020.546672
GGATCCG413750.019.4172615
ATGGATC436050.018.9605773
TCCGGCG757650.017.8233363
TCGAGGG514650.017.4004424
TGGATCC503150.016.378034
GCGCCGT821850.016.2766749
CGGCGCG854900.015.7210925
AGGGCGC784250.014.8461492
CGTCGAC786300.014.68846512-13
GAGGGCG820900.014.4657931
CCGGCGC941850.014.3793344
Produced by FastQC (version 0.11.5)