FastQCFastQC Report
Wed 25 May 2016
SRR445731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37288388
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA231550.049.603281
TCTCGAG289250.039.8934362
CTCGAGG381650.030.1818143
TCGAGGG455000.025.296144
GATCCGG319250.021.7774561
TATCTCG109850.019.7439882
CGAGGGC604800.019.0932925
ATCCGGC371350.018.6719632
AGGGCGC617900.018.5298547
GGGCGCG684600.016.7594498
GAGGGCG698400.016.468086
TCCGGCG453300.015.4559643
GGATCCG235150.014.5138412
GCGCCGT475050.014.4396979
CGGCGCG504450.013.7088315
ATATCTC170650.013.5436321
CGTCGAC450550.012.92435412-13
GGCGCGC1136200.012.8271919
CCGGCGC574350.012.215084
GCCGTCG487750.012.1198710-11
Produced by FastQC (version 0.11.5)