##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445731_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37288388
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30866826423282	33.0	31.0	34.0	28.0	34.0
2	31.64125367929555	34.0	31.0	34.0	28.0	34.0
3	31.736793663485802	34.0	31.0	34.0	28.0	34.0
4	35.12657626819373	37.0	35.0	37.0	32.0	37.0
5	34.88465063171945	37.0	35.0	37.0	32.0	37.0
6	34.791753561457256	37.0	35.0	37.0	32.0	37.0
7	34.73564228627958	37.0	35.0	37.0	32.0	37.0
8	34.68534700400564	37.0	35.0	37.0	32.0	37.0
9	36.25869943747635	39.0	37.0	39.0	32.0	39.0
10-11	36.177711919324594	39.0	37.0	39.0	32.0	39.0
12-13	36.100435945367224	39.0	37.0	39.0	32.0	39.0
14-15	37.289627028124684	40.0	38.0	41.0	31.0	41.0
16-17	37.19085229696709	40.0	38.0	41.0	31.0	41.0
18-19	37.098394277596555	40.0	38.0	41.0	31.0	41.0
20-21	36.98855426520449	40.0	37.5	41.0	31.0	41.0
22-23	36.81653406417033	40.0	37.0	41.0	30.0	41.0
24-25	36.61810970482286	40.0	37.0	41.0	30.0	41.0
26-27	36.34083688466232	40.0	36.5	41.0	29.5	41.0
28-29	36.20450008458397	40.0	36.0	41.0	29.0	41.0
30-31	36.040277217132584	40.0	36.0	41.0	28.0	41.0
32-33	36.05690825251014	40.0	36.0	41.0	28.5	41.0
34-35	35.878124109307166	40.0	35.5	41.0	27.0	41.0
36-37	35.68205426311269	40.0	35.0	41.0	26.5	41.0
38-39	35.451133352828236	40.0	35.0	41.0	25.5	41.0
40-41	35.13301816640612	39.0	35.0	41.0	24.0	41.0
42-43	34.79934715869187	39.0	35.0	41.0	22.5	41.0
44-45	34.466317409591426	39.0	34.5	41.0	20.0	41.0
46-47	34.10499658767765	39.0	34.0	41.0	15.5	41.0
48-49	33.69426650999233	38.5	33.0	40.5	10.5	41.0
50-51	33.35738730513103	38.0	33.0	40.0	7.5	41.0
52-53	33.08269247251987	38.0	33.0	40.0	2.0	41.0
54-55	32.68918207190936	38.0	32.0	40.0	2.0	41.0
56-57	32.27914715701843	37.5	31.5	40.0	2.0	41.0
58-59	31.848257894656108	37.0	31.0	40.0	2.0	41.0
60-61	31.4087979882638	36.5	30.5	40.0	2.0	41.0
62-63	30.94174108572352	36.0	29.5	39.5	2.0	41.0
64-65	30.417744137933774	35.5	29.0	39.0	2.0	41.0
66-67	29.868596920843025	35.0	28.0	39.0	2.0	40.5
68-69	29.295495772034982	35.0	27.5	38.5	2.0	40.0
70-71	28.694939172484474	34.0	26.0	37.5	2.0	40.0
72-73	28.088644674047053	34.0	25.5	37.0	2.0	39.5
74-75	27.48747577932304	34.0	24.5	36.0	2.0	39.0
76-77	26.757691697479654	32.5	24.0	35.5	2.0	38.0
78-79	26.869634683591045	34.0	24.0	35.5	2.0	38.0
80-81	26.422045235637434	33.0	22.0	35.0	2.0	37.0
82-83	25.817452232046072	33.0	19.0	35.0	2.0	37.0
84-85	25.24866073588378	32.5	16.5	35.0	2.0	36.0
86-87	24.6795592638652	32.0	7.5	35.0	2.0	36.0
88-89	24.153152396933866	32.0	2.0	35.0	2.0	35.5
90-91	23.565890485799493	31.0	2.0	35.0	2.0	35.0
92-93	22.93259491399843	31.0	2.0	34.0	2.0	35.0
94-95	22.215012754104578	30.5	2.0	34.0	2.0	35.0
96-97	21.437614251385714	30.0	2.0	34.0	2.0	35.0
98-99	20.695516859028608	29.0	2.0	34.0	2.0	35.0
100	19.546825113491096	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03643817143795047
1101	2	0.05571501335989382
1101	3	0.07503387203701806
1101	4	0.10891633230135511
1101	5	0.09548808361138583
1101	6	0.11795856627060175
1101	7	0.09699411231951416
1101	8	0.12306467398167342
1101	9	0.13348468279304626
1101	10-11	0.15847198663851714
1101	12-13	0.11013941034512698
1101	14-15	0.09291639192515788
1101	16-17	0.05480965551863903
1101	18-19	0.029908414348206236
1101	20-21	0.03665440498527062
1101	22-23	0.004087127930610279
1101	24-25	0.002072753055585963
1101	26-27	0.024427552403103903
1101	28-29	-0.01275388602957861
1101	30-31	-0.01328064266954243
1101	32-33	-0.09073936965899065
1101	34-35	-0.012004698911290745
1101	36-37	-0.017665659744146467
1101	38-39	-0.07752751392066415
1101	40-41	-0.09836729480650064
1101	42-43	-0.14501348856577323
1101	44-45	-0.26234806503578767
1101	46-47	-0.27717262409214527
1101	48-49	-0.4228707999989325
1101	50-51	-0.37893807045331585
1101	52-53	-0.44068365300142176
1101	54-55	-0.49247658133413097
1101	56-57	-0.5715689129421122
1101	58-59	-0.5769549169691892
1101	60-61	-0.5871392124890953
1101	62-63	-0.610520607160332
1101	64-65	-0.6782576968676182
1101	66-67	-0.7225545160454736
1101	68-69	-0.7066321896726713
1101	70-71	-0.7926096609830822
1101	72-73	-0.8103659992171757
1101	74-75	-0.7195228507560252
1101	76-77	-0.6472862044542076
1101	78-79	-0.7026434357353786
1101	80-81	-0.7556902705756983
1101	82-83	-0.7660938725907158
1101	84-85	-0.8115588088598535
1101	86-87	-0.8287199871818096
1101	88-89	-0.9996714738399248
1101	90-91	-1.0340384371236055
1101	92-93	-1.0020972042428973
1101	94-95	-1.014800012157579
1101	96-97	-0.9679173396237282
1101	98-99	-1.044579391714798
1101	100	-0.8676248643606996
1102	1	0.18985512620740863
1102	2	0.14706090879013445
1102	3	0.16777124872475468
1102	4	0.1470382418266709
1102	5	0.10171034515680333
1102	6	0.16587006223817014
1102	7	0.16097005062997027
1102	8	0.18474620366870198
1102	9	0.2344511189395817
1102	10-11	0.1988649552231152
1102	12-13	0.194850342695986
1102	14-15	0.1290208864060176
1102	16-17	0.12645913697339495
1102	18-19	0.10099820592296282
1102	20-21	0.10895798222509967
1102	22-23	0.1496229493276502
1102	24-25	0.19280613243798683
1102	26-27	0.10091768836944226
1102	28-29	0.1598954113710036
1102	30-31	0.18409761623188814
1102	32-33	0.07944566641032225
1102	34-35	0.17891433499512743
1102	36-37	0.16574290693544924
1102	38-39	0.22012976164325693
1102	40-41	0.24711689588539087
1102	42-43	0.18549925343061346
1102	44-45	0.07538297039913289
1102	46-47	0.05512154065425534
1102	48-49	0.05860114513959047
1102	50-51	0.014145039904278178
1102	52-53	-0.13515577451268967
1102	54-55	-0.0546293008238905
1102	56-57	-0.002517790374760409
1102	58-59	-0.03470287337526301
1102	60-61	-0.07481011886087074
1102	62-63	-0.16554666965462062
1102	64-65	-0.1719040108818639
1102	66-67	-0.19753037779302574
1102	68-69	-0.16240770859495512
1102	70-71	-0.27582832311533423
1102	72-73	-0.28906030546328765
1102	74-75	-0.2265602495690615
1102	76-77	-0.14527192183538418
1102	78-79	-0.16842327755514575
1102	80-81	-0.3024919184946313
1102	82-83	-0.2829345671568184
1102	84-85	-0.27969447407728154
1102	86-87	-0.3148532249279121
1102	88-89	-0.47798845132082945
1102	90-91	-0.44857363199048095
1102	92-93	-0.3830004806317753
1102	94-95	-0.4606259214983055
1102	96-97	-0.5148131135089109
1102	98-99	-0.6009468417045198
1102	100	-0.43546532805981286
1103	1	-0.5729088266156772
1103	2	-0.3146659300946837
1103	3	-0.3404890074133533
1103	4	-0.24067716504936243
1103	5	-0.1907305033495632
1103	6	-0.16747026777848362
1103	7	-0.22014201404456912
1103	8	-0.18244842656227434
1103	9	-0.23251593371665535
1103	10-11	-0.20887712060057595
1103	12-13	-0.17020725369138745
1103	14-15	-0.14451827551467744
1103	16-17	-0.21085449348723273
1103	18-19	-0.228679100562303
1103	20-21	-0.250854965643704
1103	22-23	-0.3412680393430776
1103	24-25	-0.28764079216778526
1103	26-27	-0.24036283733541808
1103	28-29	-0.32460769548026747
1103	30-31	-0.2644885798431531
1103	32-33	-0.2569637998285188
1103	34-35	-0.17386793062860306
1103	36-37	-0.17665166314045422
1103	38-39	-0.10034587170932951
1103	40-41	-0.07536110371078308
1103	42-43	-0.16851193996119207
1103	44-45	-0.29208077678255506
1103	46-47	-0.22929931310189033
1103	48-49	-0.7364269861907076
1103	50-51	-0.6285354493030013
1103	52-53	-0.44128678203946237
1103	54-55	-0.5116521185908738
1103	56-57	-0.4629170161444307
1103	58-59	-0.4828032342736073
1103	60-61	-0.43939744398257474
1103	62-63	-0.5055796360311753
1103	64-65	-0.5520097451219286
1103	66-67	-0.5838271834840434
1103	68-69	-0.5521754247677997
1103	70-71	-0.6230327670470537
1103	72-73	-0.6879517519040164
1103	74-75	-0.6025455206242682
1103	76-77	-0.5340056801916049
1103	78-79	-0.5300434423566927
1103	80-81	-0.6251461780985395
1103	82-83	-0.6100126818720604
1103	84-85	-0.522365624351572
1103	86-87	-0.5676180510415172
1103	88-89	-0.7724960351376886
1103	90-91	-0.6843909042279677
1103	92-93	-0.6449942304330385
1103	94-95	-0.7053527613040629
1103	96-97	-0.7130321960089923
1103	98-99	-0.8950358822797924
1103	100	-0.8191676589602892
1104	1	-0.0620488829262591
1104	2	-0.018024505339184316
1104	3	0.007823850737764815
1104	4	0.025509147299842994
1104	5	0.04270793577599363
1104	6	-0.00785041773519879
1104	7	0.03094539524409612
1104	8	-0.020644648326538118
1104	9	-0.03694662760253209
1104	10-11	-0.05799053887769645
1104	12-13	-0.021028107349408742
1104	14-15	0.001621803206724337
1104	16-17	-0.02718543340739643
1104	18-19	-0.01988819425790922
1104	20-21	-0.017334406195232077
1104	22-23	-0.05158910805432271
1104	24-25	-0.04859421646934692
1104	26-27	-0.0076327198324506185
1104	28-29	-0.06962385553482164
1104	30-31	-0.010339081542774409
1104	32-33	-0.17133516855501085
1104	34-35	0.06566541121667058
1104	36-37	0.007615241795058125
1104	38-39	0.1341230417093371
1104	40-41	0.16246897155379258
1104	42-43	0.15464094852645616
1104	44-45	0.050304925610525686
1104	46-47	0.048878739983869934
1104	48-49	0.07427103413947123
1104	50-51	0.11994315411931922
1104	52-53	0.09009312819414106
1104	54-55	0.06165728062180875
1104	56-57	0.09597609148332253
1104	58-59	0.1372619600113545
1104	60-61	0.13028657849736192
1104	62-63	0.11937538896873079
1104	64-65	0.1288419742708058
1104	66-67	0.06546606053600001
1104	68-69	0.12006509081020411
1104	70-71	0.16907801646555143
1104	72-73	0.0966803855386047
1104	74-75	0.09247434603467752
1104	76-77	0.09500377822426032
1104	78-79	0.07152112906562635
1104	80-81	0.033860160572309894
1104	82-83	0.12745747222780324
1104	84-85	0.08764477524746894
1104	86-87	0.07369559278567195
1104	88-89	-0.006548839046285337
1104	90-91	0.12375873714371366
1104	92-93	0.20868867658150592
1104	94-95	0.12434825863963539
1104	96-97	0.08131832480058065
1104	98-99	0.1615701707519861
1104	100	0.18123663916022892
1105	1	-0.10558076202607936
1105	2	-0.10965654333239883
1105	3	-0.07062049066966125
1105	4	-0.049681617927113564
1105	5	-0.04920577255702341
1105	6	-0.06045195755240229
1105	7	-0.04333647445618283
1105	8	-0.06511853128223066
1105	9	-0.1761296358370359
1105	10-11	-0.13587859221916432
1105	12-13	-0.15422827906171932
1105	14-15	-0.0716071697089049
1105	16-17	-0.06716576908061711
1105	18-19	-0.11457360593966825
1105	20-21	-0.08089302092555073
1105	22-23	-0.044609400820014855
1105	24-25	-0.016725104244727618
1105	26-27	-0.021091399982729797
1105	28-29	0.01444792106847359
1105	30-31	-0.00482294329371058
1105	32-33	-0.01461975481602451
1105	34-35	0.029515570324576856
1105	36-37	-0.13014283760443135
1105	38-39	-0.11298224956397718
1105	40-41	-0.08412428175618203
1105	42-43	0.04349386036400915
1105	44-45	-0.10216473894134026
1105	46-47	-0.02829283587531961
1105	48-49	-0.10342275607165163
1105	50-51	-0.13961370263261585
1105	52-53	-0.03979949565167118
1105	54-55	-0.21514745616022424
1105	56-57	-0.044671414524188435
1105	58-59	0.09275327302252023
1105	60-61	0.08679951742612602
1105	62-63	0.032566831193580725
1105	64-65	0.01280656898081034
1105	66-67	0.026247600803468885
1105	68-69	0.17503360018685044
1105	70-71	0.18032777388030397
1105	72-73	0.18989717116831883
1105	74-75	0.15366892636492935
1105	76-77	0.1152999657304079
1105	78-79	0.18747114343513616
1105	80-81	0.16524547371126275
1105	82-83	0.09149199066903435
1105	84-85	0.04987825857675432
1105	86-87	-0.018175062672792563
1105	88-89	0.07757906684009086
1105	90-91	0.19658995357464448
1105	92-93	0.35338552624632413
1105	94-95	0.21222064001339902
1105	96-97	0.11608592479772639
1105	98-99	0.16800640860616056
1105	100	0.21023024130279921
1106	1	-0.1862434517021221
1106	2	-0.1610258241317446
1106	3	-0.18005385426525677
1106	4	-0.17594547914404757
1106	5	-0.20536092472931244
1106	6	-0.17988792052234714
1106	7	-0.22361682043432296
1106	8	-0.18580520989971916
1106	9	-0.22235280928677525
1106	10-11	-0.21259763336339432
1106	12-13	-0.2693489098678299
1106	14-15	-0.26817572945527957
1106	16-17	-0.30541672239530726
1106	18-19	-0.28339183947758784
1106	20-21	-0.3034583494716543
1106	22-23	-0.29466082286068485
1106	24-25	-0.31774983834680626
1106	26-27	-0.3442048883590587
1106	28-29	-0.28626498276841517
1106	30-31	-0.2515053417883806
1106	32-33	-0.23675948319235118
1106	34-35	-0.27097794743583137
1106	36-37	-0.1684973949690587
1106	38-39	-0.11423824162189788
1106	40-41	-0.05351359552125956
1106	42-43	-0.1369358088256547
1106	44-45	-0.16277128327672585
1106	46-47	-0.1141505141165311
1106	48-49	-0.028341140093175454
1106	50-51	-0.06216192116339414
1106	52-53	-0.20809104612023788
1106	54-55	-0.16284593935350955
1106	56-57	-0.036517230593844374
1106	58-59	0.03606727178358682
1106	60-61	0.04706400922900755
1106	62-63	0.042645208232791276
1106	64-65	0.009403851601916813
1106	66-67	0.07498703462180956
1106	68-69	0.09759603956474194
1106	70-71	0.17871128862417862
1106	72-73	0.21368936530946314
1106	74-75	0.2483681609849242
1106	76-77	0.28486429666057944
1106	78-79	0.250592334900805
1106	80-81	0.21412088758531667
1106	82-83	0.33217654472753466
1106	84-85	0.3741975517816485
1106	86-87	0.39132791150633395
1106	88-89	0.27615249047292423
1106	90-91	0.34677451424479244
1106	92-93	0.4598207272959627
1106	94-95	0.3626023748496152
1106	96-97	0.34461223112886685
1106	98-99	0.3545760497431516
1106	100	0.27503474662641736
1107	1	-0.2611364467745041
1107	2	-0.2555386492851923
1107	3	-0.24450428391105206
1107	4	-0.27707344050708826
1107	5	-0.29925289185435133
1107	6	-0.5305512500113707
1107	7	-0.3730834334589588
1107	8	-0.4209855412660204
1107	9	-0.4364209005138804
1107	10-11	-0.4706289652103095
1107	12-13	-0.372872427530595
1107	14-15	-0.4199283018570057
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2207	92-93	0.3781675027655318
2207	94-95	0.3028972098552387
2207	96-97	0.4084354785800137
2207	98-99	0.5362614463811788
2207	100	0.5232385350515116
2208	1	-0.34204133143854776
2208	2	-0.5124184421635221
2208	3	-0.3738205345207426
2208	4	-0.37706631663401424
2208	5	-0.4240615268671917
2208	6	-0.4053059578760809
2208	7	-0.49158202742611934
2208	8	-0.4522511656897308
2208	9	-0.4116007637162369
2208	10-11	-0.47508025500317785
2208	12-13	-0.495775715816265
2208	14-15	-0.5157107270284627
2208	16-17	-0.4276664377444135
2208	18-19	-0.38594772840983893
2208	20-21	-0.4186012107912376
2208	22-23	-0.43374970658265255
2208	24-25	-0.43366356116514027
2208	26-27	-0.4330894745281242
2208	28-29	-0.349521921988746
2208	30-31	-0.3024145744497062
2208	32-33	-0.3218865543095575
2208	34-35	-0.5633875225293963
2208	36-37	-0.5457080919364685
2208	38-39	-0.5744662061685872
2208	40-41	-0.47864405926000586
2208	42-43	-0.38812803575502386
2208	44-45	-0.4374869745804091
2208	46-47	-0.6047897629159138
2208	48-49	-0.3744121540219112
2208	50-51	-0.2806976980643583
2208	52-53	-0.2156847390164316
2208	54-55	-0.11442502092290141
2208	56-57	-0.06922557787980566
2208	58-59	-0.26441127516624974
2208	60-61	-0.1046565810457345
2208	62-63	-0.08070616700396016
2208	64-65	-0.0741235064868988
2208	66-67	0.11023313822826353
2208	68-69	0.08512990389222352
2208	70-71	0.13564951997793173
2208	72-73	0.2832859246368642
2208	74-75	0.38504393778396917
2208	76-77	0.4149848381633561
2208	78-79	0.508760861954741
2208	80-81	0.5902638183926356
2208	82-83	0.6271319953465131
2208	84-85	0.6799350905047916
2208	86-87	0.8349805172152536
2208	88-89	0.8816453357601794
2208	90-91	1.1034477238162026
2208	92-93	1.2003829584052887
2208	94-95	1.2448399003307458
2208	96-97	1.283342106064783
2208	98-99	1.2088079136922687
2208	100	1.0856418190037793
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	658682.0
3	227703.0
4	160531.0
5	112162.0
6	114392.0
7	117568.0
8	122815.0
9	141208.0
10	158496.0
11	175260.0
12	198763.0
13	258194.0
14	332769.0
15	375560.0
16	452344.0
17	463925.0
18	420172.0
19	380289.0
20	380995.0
21	399438.0
22	426921.0
23	459950.0
24	498838.0
25	545825.0
26	605636.0
27	681244.0
28	772858.0
29	885081.0
30	1019299.0
31	1183085.0
32	1396869.0
33	1699239.0
34	2146311.0
35	2841358.0
36	4019564.0
37	5785071.0
38	5560327.0
39	1108413.0
40	1233.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.550389707052336	27.894779485008563	28.650160874272345	22.904669933666753
2	23.450787091518833	27.37702271726507	27.247936618092268	21.924253573123824
3	23.236111526804386	26.402983572767297	27.209074514440758	23.151830385987566
4	23.70306891149346	26.06451590061804	27.218338905561644	23.014076282326855
5	24.089710531772404	26.3575324690198	26.993198065654074	22.55955893355372
6	24.111604207449343	26.433312889562373	26.17104894790423	23.28403395508406
7	24.111155489747464	26.782698720771126	26.493747204555056	22.612398584926353
8	23.767193831787853	26.618995627534026	26.592602353965518	23.02120818671261
9	23.415534258224614	26.29721111349331	26.879154229248403	23.408100399033668
10-11	23.362425420008385	26.509207237225752	26.709242715849584	23.41912462691628
12-13	23.111139616635324	26.610237002094955	26.854167254660204	23.424456126609513
14-15	22.937754775066534	26.78089278494357	27.179449340826363	23.101903099163536
16-17	22.799408818188923	26.697279181368827	27.416411062452433	23.086900937989814
18-19	22.451864622962606	26.6589049037185	27.98005357658061	22.909176896738288
20-21	22.208228609030982	26.41976588564833	28.54527928261955	22.826726222701133
22-23	22.224118890379533	26.371753264638386	28.887041574410667	22.517086270571415
24-25	22.152859399987793	26.292947500012687	29.22739285244058	22.326800247558936
26-27	22.246760293658124	26.309940879926195	29.22330167912714	22.219997147288538
28-29	22.242977718155476	26.38335421433074	29.18414546991592	22.18952259759787
30-31	22.22989202637722	26.349129165865005	29.14546495598092	22.275513851776854
32-33	22.235211502383457	26.391045523104072	29.09255879595807	22.281184178554405
34-35	22.288207552374438	26.441699722838617	29.067419394043075	22.20267333074387
36-37	22.293682936739582	26.416298554199262	28.9969422455573	22.293076263503856
38-39	22.347553496920295	26.458452479492163	28.874913567834952	22.31908045575259
40-41	22.410452635443985	26.573859655630056	28.707973009338396	22.30771469958757
42-43	22.517168689384796	26.639729302970196	28.35816775910849	22.48493424853652
44-45	22.60399719112625	26.789935484051945	28.02140851844857	22.58465880637323
46-47	22.809988563585627	26.954320615731014	27.594781943212737	22.640908877470626
48-49	22.891967341958523	26.97619647678846	27.293363289292405	22.838472891960613
50-51	22.91286853026831	27.03296431230872	27.138145537447294	22.91602161997568
52-53	22.98725370544778	27.074550363395637	27.034930685480113	22.903265245676472
54-55	23.006865253937754	27.065601740157515	26.949272809042224	22.978260196862507
56-57	23.02527536836407	27.077005488232253	26.938718180535442	22.959000962868238
58-59	23.100828922409182	27.12458050881085	26.87991020345897	22.894680365320998
60-61	23.049849478429874	27.103663856419324	26.84625408259888	23.00023258255192
62-63	23.097647108382137	27.150203322313597	26.833581882267964	22.918567687036298
64-65	23.149641260136505	27.22058423301305	26.75545713241845	22.874317374431996
66-67	23.1814852093429	27.225575546638936	26.65508445312262	22.93785479089555
68-69	23.211235478170614	27.286283494538928	26.566366180921047	22.936114846369414
70-71	23.297082453234292	27.361344199253868	26.478852060261943	22.862721287249897
72-73	23.29042248360568	27.38779103825873	26.38928085441551	22.932505623720083
74-75	23.38521418153729	27.445061273144322	26.32473495900455	22.84498958631383
76-77	23.17619407654454	27.62172728021646	26.22369981636777	22.97837882687123
78-79	23.252213289789314	27.656465697318787	26.11898719392583	22.972333818966067
80-81	23.28072899188329	27.699708232940278	26.004682085207513	23.01488068996892
82-83	23.293982288549884	27.798980677507373	25.942295591346337	22.964741442596402
84-85	23.30285690561673	27.82334732415763	25.831309644249643	23.042486125975994
86-87	23.348373526526107	27.909955003866198	25.704526375927443	23.037145093680248
88-89	23.43703607070956	27.991141826616673	25.536296141354107	23.035525961319657
90-91	23.42808696909308	27.999538919448124	25.442530521208628	23.129843590250168
92-93	23.48065071095872	28.06661187017079	25.32971798935793	23.12301942951256
94-95	23.539210880984154	28.127814314852767	25.185064282261948	23.14791052190113
96-97	23.513021514704853	28.1768498090489	25.025249372336344	23.284879303909907
98-99	23.575128901378488	28.210301564400158	24.885318929429133	23.329250604792218
100	24.23651819313054	28.108299717595347	24.018400648889436	23.636781440384677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1842.0
1	1345.0
2	839.0
3	859.0
4	954.5
5	1124.0
6	1333.5
7	1607.0
8	1912.5
9	2239.0
10	2787.5
11	3543.0
12	4415.5
13	6022.0
14	10487.0
15	14855.0
16	20874.0
17	24815.0
18	26844.0
19	35011.0
20	56172.5
21	70870.5
22	78015.0
23	96787.0
24	120987.5
25	151122.0
26	187606.5
27	232104.0
28	283319.5
29	337167.5
30	393991.0
31	455481.5
32	523167.5
33	599012.5
34	680205.0
35	768224.0
36	867498.5
37	981400.5
38	1089096.5
39	1162359.5
40	1218494.5
41	1289147.5
42	1390785.5
43	1480033.5
44	1541004.5
45	1578949.5
46	1559926.5
47	1499600.5
48	1448587.0
49	1428613.5
50	1387027.0
51	1308349.0
52	1205794.5
53	1087493.5
54	992597.0
55	919892.0
56	840665.0
57	758121.0
58	689887.0
59	629777.0
60	563382.0
61	497942.0
62	439160.0
63	382983.5
64	331471.0
65	282997.5
66	239169.5
67	200546.0
68	165696.5
69	134863.0
70	108334.5
71	86932.5
72	69811.0
73	56429.0
74	45438.5
75	35111.0
76	26608.0
77	20518.0
78	15949.0
79	11912.5
80	8103.0
81	5082.0
82	3118.0
83	1878.5
84	1097.5
85	665.5
86	416.5
87	260.5
88	151.0
89	91.5
90	63.5
91	36.0
92	16.0
93	11.5
94	6.5
95	5.0
96	3.5
97	2.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07155310655960778
2	0.021036039423318595
3	0.0034327040364415863
4	0.012550824133239548
5	0.010316884709524049
6	0.05010138813187633
7	0.042101578646950355
8	0.057167931206894756
9	0.10700918473600951
10-11	0.12401850141658041
12-13	0.08702843362389386
14-15	0.12132999688803925
16-17	0.1624768547248543
18-19	0.16940394419839228
20-21	0.17660055457479149
22-23	0.19846929290695003
24-25	0.13296096361151358
26-27	0.19084895812605254
28-29	0.16080609330711748
30-31	0.13604369274423986
32-33	0.1719114272250117
34-35	0.4697776154871592
36-37	0.5387132852189802
38-39	0.5617150840631673
40-41	0.5476999971143832
42-43	0.5549502434913518
44-45	0.5751227433055031
46-47	0.5957283538242523
48-49	0.34700615108381727
50-51	0.020100091213382566
52-53	0.02057208801839329
54-55	0.031544672834878246
56-57	0.03705577189338407
58-59	0.025333623968941753
60-61	0.007246223676925911
62-63	0.016966407880115386
64-65	0.007148337975886756
66-67	0.013600748844385549
68-69	0.007845605983288954
70-71	0.020236863014834537
72-73	0.003317386635217377
74-75	0.006971339174007736
76-77	0.011683261824029508
78-79	0.0053113049563848136
80-81	0.007108110975459706
82-83	0.009452004200342477
84-85	0.006763499671801312
86-87	0.008242512387502511
88-89	0.00878155419322498
90-91	0.005528530758690883
92-93	0.005204032955246014
94-95	0.011031584417111301
96-97	0.014167949550406952
98-99	0.0098743877048265
100	0.006782272272000602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.7288388E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.30365816514908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80170317593925	36.025034685250844
2	11.896931050428709	11.017428571467978
3	4.04157772837891	5.614195007482104
4	1.8509271196463402	3.4281878654683253
5	1.0315467050965663	2.388219300708863
6	0.6441525779974988	1.7895972466677432
7	0.4348372987783821	1.4094190348063702
8	0.3061851584651815	1.1341994330251015
9	0.22940671643975835	0.9560133160934271
>10	1.4671891641526946	13.760613458875376
>50	0.15664676980159747	4.878804303520106
>100	0.1238915073737596	12.222512727218517
>500	0.014144224293183107	4.209427284164601
>1k	7.680959075060474E-4	0.5506395962198983
>5k	2.3176825232263647E-5	0.08192056879640801
>10k+	6.953047559652257E-5	0.5337876002342965
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.681800028469989E-6
2	0.0	0.0	0.0	2.681800028469989E-6	2.681800028469989E-6
3	0.0	0.0	0.0	5.363600056939978E-6	5.363600056939978E-6
4	0.0	2.681800028469989E-6	0.0	5.363600056939978E-6	5.363600056939978E-6
5	0.0	2.681800028469989E-6	0.0	8.045400085409967E-6	5.363600056939978E-6
6	0.0	8.045400085409967E-6	0.0	8.045400085409967E-6	5.363600056939978E-6
7	2.681800028469989E-6	8.045400085409967E-6	0.0	1.0727200113879956E-5	5.363600056939978E-6
8	5.363600056939978E-6	1.3409000142349946E-5	0.0	1.0727200113879956E-5	8.045400085409967E-6
9	1.0727200113879956E-5	1.3409000142349946E-5	0.0	1.3409000142349946E-5	8.045400085409967E-6
10-11	5.229510055516479E-5	2.0113500213524917E-5	0.0	1.8772600199289922E-5	1.0727200113879956E-5
12-13	2.4136200256229902E-4	2.5477100270464898E-5	0.0	2.145440022775991E-5	1.206810012811495E-5
14-15	7.750402082278269E-4	3.218160034163987E-5	0.0	2.681800028469989E-5	1.3409000142349946E-5
16-17	0.0017257383183204382	3.4863400370109856E-5	0.0	3.888610041281484E-5	1.6090800170819934E-5
18-19	0.003223523634220927	4.1567900441284825E-5	0.0	4.424970046975482E-5	1.7431700185054928E-5
20-21	0.005094079154078744	4.559060048398981E-5	0.0	4.559060048398981E-5	1.8772600199289922E-5
22-23	0.007648493681196409	5.229510055516479E-5	0.0	4.69315004982248E-5	1.8772600199289922E-5
24-25	0.010677586813353263	5.899960062633976E-5	0.0	5.899960062633976E-5	2.0113500213524917E-5
26-27	0.014332880252157856	6.570410069751473E-5	0.0	6.168140065480975E-5	2.145440022775991E-5
28-29	0.01771999368811545	7.777220082562969E-5	0.0	6.838590072598473E-5	2.145440022775991E-5
30-31	0.020513088417766945	8.715850092527464E-5	0.0	7.777220082562969E-5	2.41362002562299E-5
32-33	0.02327668334710527	9.386300099644962E-5	0.0	7.777220082562969E-5	2.41362002562299E-5
34-35	0.026317844579390237	9.386300099644962E-5	0.0	8.715850092527464E-5	2.41362002562299E-5
36-37	0.02960707231430868	1.0190840108185958E-4	0.0	9.386300099644962E-5	2.6818000284699893E-5
38-39	0.03262409734633742	1.0459020111032957E-4	0.0	9.78857010391546E-5	2.6818000284699893E-5
40-41	0.035600895377939104	1.0861290115303455E-4	0.0	1.0459020111032957E-4	2.949980031316988E-5
42-43	0.038766760311547924	1.1129470118150455E-4	0.0	1.0727200113879957E-4	2.949980031316988E-5
44-45	0.0426164842524166	1.1665830123844453E-4	0.0	1.0861290115303455E-4	2.949980031316988E-5
46-47	0.04759122330522843	1.233628013096195E-4	0.0	1.1397650120997455E-4	2.949980031316988E-5
48-49	0.053501910567976284	1.2872640136655947E-4	0.0	1.1934010126691451E-4	3.3522500355874865E-5
50-51	0.06059258984325094	1.3543090143773446E-4	0.0	1.220219012953845E-4	3.4863400370109856E-5
52-53	0.06947873423758624	1.3811270146620443E-4	0.0	1.2872640136655947E-4	3.4863400370109856E-5
54-55	0.0805626137552527	1.4347630152314442E-4	0.0	1.3274910140926446E-4	3.4863400370109856E-5
56-57	0.09501215230864901	1.461581015516144E-4	0.0	1.3677180145196946E-4	3.7545200398579845E-5
58-59	0.1131585522012912	1.5420350163702437E-4	0.0	1.3811270146620443E-4	3.7545200398579845E-5
60-61	0.13544565133789105	1.6358980173666933E-4	0.0	1.4213540150890942E-4	4.1567900441284825E-5
62-63	0.16336721233430634	1.6627160176513933E-4	0.0	1.474990015658494E-4	4.290880045551982E-5
64-65	0.1981434542034909	1.743170018505493E-4	0.0	1.5152170160855438E-4	4.290880045551982E-5
66-67	0.2412842303614734	1.743170018505493E-4	0.0	1.5420350163702437E-4	4.290880045551982E-5
68-69	0.2927439502077698	1.7968060190748928E-4	2.681800028469989E-6	1.5554440165125937E-4	4.559060048398981E-5
70-71	0.35512395976999594	1.8772600199289924E-4	2.681800028469989E-6	1.6761250177937432E-4	4.559060048398981E-5
72-73	0.42975309096225883	1.930896020498392E-4	2.681800028469989E-6	1.6895340179360932E-4	4.69315004982248E-5
74-75	0.5182645600018966	2.0381680216371919E-4	2.681800028469989E-6	1.6895340179360932E-4	5.095420054092979E-5
76-77	0.6218303135013505	2.0649860219218915E-4	2.681800028469989E-6	1.743170018505493E-4	5.095420054092979E-5
78-79	0.7421063629782012	2.1052130223489415E-4	2.681800028469989E-6	1.7968060190748928E-4	5.229510055516479E-5
80-81	0.879718908739096	2.1454400227759911E-4	2.681800028469989E-6	1.8638510197866424E-4	5.3636000569399785E-5
82-83	1.0362193721005049	2.185667023203041E-4	2.681800028469989E-6	1.930896020498392E-4	5.899960062633976E-5
84-85	1.213104197478314	2.239303023772441E-4	2.681800028469989E-6	1.9979410212101416E-4	6.168140065480975E-5
86-87	1.4104618842734635	2.2929390243418406E-4	2.681800028469989E-6	2.0381680216371916E-4	6.302230066904474E-5
88	1.5725056282937198	2.3868020253382902E-4	2.681800028469989E-6	2.0381680216371916E-4	6.436320068327973E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	23155	0.0	49.60328	1
TCTCGAG	28925	0.0	39.893436	2
CTCGAGG	38165	0.0	30.181814	3
TCGAGGG	45500	0.0	25.29614	4
GATCCGG	31925	0.0	21.777456	1
TATCTCG	10985	0.0	19.743988	2
CGAGGGC	60480	0.0	19.093292	5
ATCCGGC	37135	0.0	18.671963	2
AGGGCGC	61790	0.0	18.529854	7
GGGCGCG	68460	0.0	16.759449	8
GAGGGCG	69840	0.0	16.46808	6
TCCGGCG	45330	0.0	15.455964	3
GGATCCG	23515	0.0	14.513841	2
GCGCCGT	47505	0.0	14.439697	9
CGGCGCG	50445	0.0	13.708831	5
ATATCTC	17065	0.0	13.543632	1
CGTCGAC	45055	0.0	12.924354	12-13
GGCGCGC	113620	0.0	12.827191	9
CCGGCGC	57435	0.0	12.21508	4
GCCGTCG	48775	0.0	12.11987	10-11
>>END_MODULE