FastQCFastQC Report
Wed 25 May 2016
SRR445730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31187123
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA180450.046.316351
TCTCGAG223150.037.1793482
CTCGAGG286500.028.8015543
TCGAGGG341700.024.0530914
GATCCGG247650.020.6777551
ATCCGGC279450.018.4652712
CGAGGGC480900.017.1857075
AGGGCGC478300.017.1134537
TATCTCG117550.016.1063691
GGGCGCG519700.015.813698
GGATCCG178500.015.5548021
GAGGGCG552600.015.0084156
TCCGGCG368400.014.1122673
GCGCCGT361700.014.1083229
CGGCGCG380800.013.4118595
ATATCTC138800.013.0312611
CGTCGAC333150.012.25313512-13
GGCGCGC858300.012.2522269
CCGGCGC444100.011.717554
CCGTCGA334200.011.66613912-13
Produced by FastQC (version 0.11.5)