##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445730_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31187123
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297556943614197	33.0	31.0	34.0	28.0	34.0
2	31.631732686596322	34.0	31.0	34.0	28.0	34.0
3	31.72908171106389	34.0	31.0	34.0	28.0	34.0
4	35.126878199056705	37.0	35.0	37.0	32.0	37.0
5	34.888861309842525	37.0	35.0	37.0	32.0	37.0
6	34.79436352625409	37.0	35.0	37.0	32.0	37.0
7	34.74066774290145	37.0	35.0	37.0	32.0	37.0
8	34.690626608937286	37.0	35.0	37.0	32.0	37.0
9	36.26165821707889	39.0	37.0	39.0	32.0	39.0
10-11	36.17826193842888	39.0	37.0	39.0	32.0	39.0
12-13	36.09928671522538	39.0	37.0	39.0	32.0	39.0
14-15	37.27632826214845	40.0	38.0	41.0	31.0	41.0
16-17	37.17593613877112	40.0	38.0	41.0	31.0	41.0
18-19	37.07758376750559	40.0	37.5	41.0	31.0	41.0
20-21	36.961400383100425	40.0	37.0	41.0	31.0	41.0
22-23	36.782214152937414	40.0	37.0	41.0	30.0	41.0
24-25	36.57559198390952	40.0	37.0	41.0	30.0	41.0
26-27	36.29286018142808	40.0	36.0	41.0	29.5	41.0
28-29	36.1605569709011	40.0	36.0	41.0	28.5	41.0
30-31	35.99639835325625	40.0	36.0	41.0	28.0	41.0
32-33	36.01849688090819	40.0	36.0	41.0	28.0	41.0
34-35	35.838533631332396	40.0	35.0	41.0	27.0	41.0
36-37	35.63809821444575	40.0	35.0	41.0	26.5	41.0
38-39	35.40389844552189	39.5	35.0	41.0	25.5	41.0
40-41	35.08477024315452	39.0	35.0	41.0	24.0	41.0
42-43	34.75613007009335	39.0	35.0	41.0	22.5	41.0
44-45	34.428840534601406	39.0	34.0	41.0	20.0	41.0
46-47	34.07282108067487	39.0	34.0	41.0	16.5	41.0
48-49	33.66229942082185	38.5	33.0	40.0	11.0	41.0
50-51	33.31657575467926	38.0	33.0	40.0	7.5	41.0
52-53	33.02968390191042	38.0	33.0	40.0	4.5	41.0
54-55	32.62321846744248	38.0	32.0	40.0	2.0	41.0
56-57	32.197281470945555	37.0	31.0	40.0	2.0	41.0
58-59	31.75098533455619	37.0	31.0	40.0	2.0	41.0
60-61	31.293772641355858	36.0	30.0	40.0	2.0	41.0
62-63	30.812119283974994	36.0	29.5	39.0	2.0	41.0
64-65	30.273370422786353	35.0	29.0	39.0	2.0	41.0
66-67	29.706727500962497	35.0	28.0	39.0	2.0	40.0
68-69	29.11812259822748	34.5	27.0	38.0	2.0	40.0
70-71	28.508413600703086	34.0	26.0	37.0	2.0	40.0
72-73	27.893396242417104	34.0	25.5	36.5	2.0	39.0
74-75	27.284236814662258	34.0	24.5	36.0	2.0	39.0
76-77	26.556584026683062	32.5	23.5	35.0	2.0	38.0
78-79	26.66881485028292	33.0	23.5	35.0	2.0	37.0
80-81	26.223821110398674	33.0	21.0	35.0	2.0	37.0
82-83	25.61978716343922	33.0	19.0	35.0	2.0	36.5
84-85	25.054789487956295	32.0	14.0	35.0	2.0	36.0
86-87	24.487643810556044	32.0	6.5	35.0	2.0	36.0
88-89	23.961518476712328	32.0	2.0	35.0	2.0	35.0
90-91	23.370519092126578	31.0	2.0	34.5	2.0	35.0
92-93	22.730904370371068	31.0	2.0	34.0	2.0	35.0
94-95	22.002973647168417	30.5	2.0	34.0	2.0	35.0
96-97	21.21559422137143	29.5	2.0	34.0	2.0	35.0
98-99	20.457660089390096	29.0	2.0	34.0	2.0	35.0
100	19.275377597350033	26.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03912772466610903
1101	2	0.07279020583254692
1101	3	0.09765883324303104
1101	4	0.1042121138747305
1101	5	0.11179390833618896
1101	6	0.14534259828412388
1101	7	0.11520677754950981
1101	8	0.14447403226817102
1101	9	0.15344176497330864
1101	10-11	0.19708575723944932
1101	12-13	0.14596656662364893
1101	14-15	0.13397676433530137
1101	16-17	0.09254168496863002
1101	18-19	0.08537816649569407
1101	20-21	0.08248684416484764
1101	22-23	0.05060905399462001
1101	24-25	0.026871436890615996
1101	26-27	0.0636851529623712
1101	28-29	0.023956848123731334
1101	30-31	0.010073509083099452
1101	32-33	-0.05416714805078726
1101	34-35	0.028137380263039802
1101	36-37	-0.009059365030154254
1101	38-39	-0.027603353607915437
1101	40-41	-0.07781815396033664
1101	42-43	-0.13325830646567738
1101	44-45	-0.2622604536393638
1101	46-47	-0.2586502023245387
1101	48-49	-0.39626939133402317
1101	50-51	-0.3239142670558621
1101	52-53	-0.40170669709381457
1101	54-55	-0.4575674186509673
1101	56-57	-0.5299866548879777
1101	58-59	-0.5104547666948562
1101	60-61	-0.5418460621680943
1101	62-63	-0.557141413615664
1101	64-65	-0.6636914948773338
1101	66-67	-0.7249539680318051
1101	68-69	-0.7256706153477204
1101	70-71	-0.8106855369606372
1101	72-73	-0.8289771289481997
1101	74-75	-0.7140279678626662
1101	76-77	-0.6323348510862203
1101	78-79	-0.7137108472012734
1101	80-81	-0.7461468713803363
1101	82-83	-0.7344462709748711
1101	84-85	-0.776843553080635
1101	86-87	-0.8346123717955827
1101	88-89	-1.0017049101008482
1101	90-91	-1.026981282195539
1101	92-93	-1.0328938715327176
1101	94-95	-1.0603162306813445
1101	96-97	-0.980916516227861
1101	98-99	-1.038726139585087
1101	100	-0.8483778142892717
1102	1	0.19039796618392302
1102	2	0.14317392609578405
1102	3	0.16962011911792274
1102	4	0.16491930561033996
1102	5	0.11896911026493484
1102	6	0.18620549913262607
1102	7	0.17000215931633988
1102	8	0.17715552395304002
1102	9	0.2245112214282372
1102	10-11	0.20140409289304273
1102	12-13	0.18578136525265165
1102	14-15	0.12558003702483944
1102	16-17	0.14261202277710794
1102	18-19	0.0901938559163753
1102	20-21	0.11271502450487247
1102	22-23	0.14359021936171246
1102	24-25	0.16287316957279074
1102	26-27	0.07857629165918922
1102	28-29	0.1579040264551992
1102	30-31	0.1745274504970027
1102	32-33	0.07270789795850874
1102	34-35	0.1561506946551603
1102	36-37	0.15037095621026708
1102	38-39	0.20298726224523023
1102	40-41	0.24833910649380186
1102	42-43	0.15742610947280156
1102	44-45	0.06586478616888769
1102	46-47	0.0251524252263593
1102	48-49	0.019900055220311685
1102	50-51	0.0028267398264887333
1102	52-53	-0.1554149976606567
1102	54-55	-0.06618335409665121
1102	56-57	-0.016910129419208886
1102	58-59	-0.025649809171401472
1102	60-61	-0.10652067310130775
1102	62-63	-0.17361558002129485
1102	64-65	-0.17277367317954173
1102	66-67	-0.2069856383069748
1102	68-69	-0.21744421403623093
1102	70-71	-0.32763319526110024
1102	72-73	-0.3451096654548955
1102	74-75	-0.2785747265362488
1102	76-77	-0.19934380240639982
1102	78-79	-0.21479509829799426
1102	80-81	-0.371877491575173
1102	82-83	-0.3402395195588497
1102	84-85	-0.34997668727713815
1102	86-87	-0.42549873913977265
1102	88-89	-0.5743075959727939
1102	90-91	-0.5356340178731713
1102	92-93	-0.4771782405412459
1102	94-95	-0.5591316829744777
1102	96-97	-0.6138104335424934
1102	98-99	-0.6874347899553186
1102	100	-0.5198558030587428
1103	1	-0.5681636460170978
1103	2	-0.30475498795326317
1103	3	-0.32965419134281504
1103	4	-0.22201161595163654
1103	5	-0.16282819458821507
1103	6	-0.1419520062105022
1103	7	-0.18732205189312623
1103	8	-0.14908135095149078
1103	9	-0.1966256557401138
1103	10-11	-0.16526324188045294
1103	12-13	-0.13429340412938728
1103	14-15	-0.11381463273945513
1103	16-17	-0.17513472781131867
1103	18-19	-0.17586043708342913
1103	20-21	-0.18828033758145324
1103	22-23	-0.294262040728249
1103	24-25	-0.23583916935054816
1103	26-27	-0.19293296011464633
1103	28-29	-0.2939736798656938
1103	30-31	-0.2610519615046414
1103	32-33	-0.2471292450994511
1103	34-35	-0.1565031778205892
1103	36-37	-0.1488090283164425
1103	38-39	-0.09788661932737597
1103	40-41	-0.07475855660566566
1103	42-43	-0.1689763551245278
1103	44-45	-0.28522665073270304
1103	46-47	-0.2217914314823517
1103	48-49	-0.7441744679369506
1103	50-51	-0.6172531562127688
1103	52-53	-0.4273916222542695
1103	54-55	-0.4947287195436516
1103	56-57	-0.5064375885612904
1103	58-59	-0.5324527471536129
1103	60-61	-0.47719195207143983
1103	62-63	-0.49838663610321277
1103	64-65	-0.556669607982375
1103	66-67	-0.5753047096996156
1103	68-69	-0.5065360168181563
1103	70-71	-0.601029818570801
1103	72-73	-0.6917921452916893
1103	74-75	-0.5609194182537642
1103	76-77	-0.5118398646709892
1103	78-79	-0.5114152253516693
1103	80-81	-0.5905004205045792
1103	82-83	-0.6071824627058291
1103	84-85	-0.5448164403939941
1103	86-87	-0.539705628094918
1103	88-89	-0.790923243662867
1103	90-91	-0.691719042888618
1103	92-93	-0.6626291624249951
1103	94-95	-0.6910567085222183
1103	96-97	-0.6764867491369024
1103	98-99	-0.8532687264972196
1103	100	-0.760284204303364
1104	1	-0.04089498554702686
1104	2	-0.010974571954935897
1104	3	0.023300278348926895
1104	4	0.037164429778279384
1104	5	0.0683465022783949
1104	6	0.016068519711275542
1104	7	0.09339506134087827
1104	8	0.011613310974851743
1104	9	1.000620213176262E-5
1104	10-11	-0.013843716381138904
1104	12-13	0.015640667638400885
1104	14-15	0.04886228646240909
1104	16-17	0.005313416313917685
1104	18-19	0.03968506537982108
1104	20-21	0.024724582330591716
1104	22-23	-0.0027765860318567093
1104	24-25	0.012029038178020812
1104	26-27	0.032498457344907195
1104	28-29	-0.028776898305359566
1104	30-31	0.05650088032324163
1104	32-33	-0.10926573688452379
1104	34-35	0.13972690901071871
1104	36-37	0.07709831472942597
1104	38-39	0.1901877513173602
1104	40-41	0.20610447431779022
1104	42-43	0.1854150215498791
1104	44-45	0.10143504186892471
1104	46-47	0.11610995657284917
1104	48-49	0.10286481389719171
1104	50-51	0.1280109399308742
1104	52-53	0.10835586092872518
1104	54-55	0.09351100925892553
1104	56-57	0.1238593394602745
1104	58-59	0.1693887323199803
1104	60-61	0.17378772895818528
1104	62-63	0.14624142553590147
1104	64-65	0.12942839144167095
1104	66-67	0.08251186337273708
1104	68-69	0.11433673174957804
1104	70-71	0.15999441418253824
1104	72-73	0.1113302111137493
1104	74-75	0.09381231041731652
1104	76-77	0.09666052241261269
1104	78-79	0.07645899522236732
1104	80-81	0.026643458836225875
1104	82-83	0.14360524460822433
1104	84-85	0.08537847040741653
1104	86-87	0.03166047036923558
1104	88-89	-0.06190947525603363
1104	90-91	0.06615432455278736
1104	92-93	0.18836301606068773
1104	94-95	0.09617661544119827
1104	96-97	0.022549419202416487
1104	98-99	0.11508183394848714
1104	100	0.14303036178798223
1105	1	-0.09360887033432164
1105	2	-0.10283198613304734
1105	3	-0.06636051772470353
1105	4	-0.048089710558379295
1105	5	-0.04779266764950307
1105	6	-0.03816018319145087
1105	7	-0.017556327942124028
1105	8	-0.033573548119292695
1105	9	-0.15203965633093475
1105	10-11	-0.10260346415459765
1105	12-13	-0.12684420621447856
1105	14-15	-0.035636308963702845
1105	16-17	-0.018886675493668292
1105	18-19	-0.05076777818084821
1105	20-21	-0.021935824354201827
1105	22-23	0.0025218043767125664
1105	24-25	0.04164521691340184
1105	26-27	0.031465592293344
1105	28-29	0.054722118687578813
1105	30-31	0.05391033751988061
1105	32-33	0.02842277742637833
1105	34-35	0.08027864307822341
1105	36-37	-0.08478192553140218
1105	38-39	-0.06412855111163651
1105	40-41	-0.04017025679289077
1105	42-43	0.09501427363673542
1105	44-45	-0.047376947184396556
1105	46-47	0.032056828660863346
1105	48-49	-0.03726223678753371
1105	50-51	-0.07273257034459846
1105	52-53	0.0057555318896262975
1105	54-55	-0.15387498436963654
1105	56-57	4.223817866559898E-4
1105	58-59	0.13824410686503086
1105	60-61	0.1474847069369467
1105	62-63	0.07941862322436322
1105	64-65	0.07091980109841955
1105	66-67	0.08351744825804275
1105	68-69	0.2330922015972483
1105	70-71	0.22461836742603936
1105	72-73	0.21815312079091598
1105	74-75	0.19668064683810016
1105	76-77	0.15851717991816372
1105	78-79	0.19968506103434436
1105	80-81	0.16308214377119867
1105	82-83	0.08859059439437189
1105	84-85	0.04738490726493794
1105	86-87	-0.03465740995514821
1105	88-89	0.039073814003753427
1105	90-91	0.18669941387492273
1105	92-93	0.3524821838889096
1105	94-95	0.21869367989129174
1105	96-97	0.1049464425660922
1105	98-99	0.1513002185445771
1105	100	0.19118980954653608
1106	1	-0.1512355096006317
1106	2	-0.12527828485149684
1106	3	-0.14661126938977276
1106	4	-0.14184553834005698
1106	5	-0.16988941375271338
1106	6	-0.12074539938191009
1106	7	-0.17412891880228187
1106	8	-0.1309251184968474
1106	9	-0.1860863156579171
1106	10-11	-0.17370441770091105
1106	12-13	-0.22486789287658127
1106	14-15	-0.21444813774828475
1106	16-17	-0.2665952681713577
1106	18-19	-0.21948160682402573
1106	20-21	-0.25159469302921167
1106	22-23	-0.23522135898104324
1106	24-25	-0.23648226567565445
1106	26-27	-0.2547480840207612
1106	28-29	-0.2065687136564094
1106	30-31	-0.16508471332087993
1106	32-33	-0.15209718232065228
1106	34-35	-0.179721435773466
1106	36-37	-0.08597385526423551
1106	38-39	-0.03438193714606541
1106	40-41	0.0024728903502335697
1106	42-43	-0.07028628064666975
1106	44-45	-0.06446217609001081
1106	46-47	-0.019849141694756156
1106	48-49	0.05259890382121313
1106	50-51	0.03924233756630713
1106	52-53	-0.08576681111444628
1106	54-55	-0.021970545272054665
1106	56-57	0.07511159546918122
1106	58-59	0.16379780451461556
1106	60-61	0.1476716740918853
1106	62-63	0.15453131814407683
1106	64-65	0.11734660772408034
1106	66-67	0.1860363425692988
1106	68-69	0.19950053938313417
1106	70-71	0.277629230797924
1106	72-73	0.30510333545717216
1106	74-75	0.3329570995859257
1106	76-77	0.38608622136041504
1106	78-79	0.38029640087341576
1106	80-81	0.32116978203743685
1106	82-83	0.4271323885309144
1106	84-85	0.48288249630686053
1106	86-87	0.4882517746016326
1106	88-89	0.34326160180588516
1106	90-91	0.41727896153826904
1106	92-93	0.5321350228999151
1106	94-95	0.42536804171149
1106	96-97	0.38554727601205485
1106	98-99	0.43707468633373736
1106	100	0.35700515337712346
1107	1	-0.25073706949940444
1107	2	-0.24178676477425043
1107	3	-0.22673146253876553
1107	4	-0.2550754854153041
1107	5	-0.27862125559722273
1107	6	-0.4930150729401461
1107	7	-0.3231900458233028
1107	8	-0.38469784921154115
1107	9	-0.3930557594677637
1107	10-11	-0.4328519493554097
1107	12-13	-0.33968447138533975
1107	14-15	-0.39163418270333494
1107	16-17	-0.4581714086212827
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2207	88-89	0.2876154122219923
2207	90-91	0.35748658615077034
2207	92-93	0.34371487216007424
2207	94-95	0.2685897918835778
2207	96-97	0.3828894299178991
2207	98-99	0.5151410400484266
2207	100	0.5018403042186748
2208	1	-0.3380323610775555
2208	2	-0.5164983522551907
2208	3	-0.38813485227074196
2208	4	-0.372948811443635
2208	5	-0.4517453250109966
2208	6	-0.40889228115591436
2208	7	-0.49825563402201567
2208	8	-0.47255657197796097
2208	9	-0.440040693978176
2208	10-11	-0.48461141657874407
2208	12-13	-0.5004354665099982
2208	14-15	-0.5168955202813876
2208	16-17	-0.4352931407775671
2208	18-19	-0.40489561827522635
2208	20-21	-0.43367911079838706
2208	22-23	-0.4511663221203648
2208	24-25	-0.44936935116196963
2208	26-27	-0.44593410410108447
2208	28-29	-0.3656445672614552
2208	30-31	-0.30570470550529194
2208	32-33	-0.32137487880863347
2208	34-35	-0.5645939528795054
2208	36-37	-0.5245492016824329
2208	38-39	-0.5745466363872822
2208	40-41	-0.4847902322007869
2208	42-43	-0.38356790994539836
2208	44-45	-0.42975393802912976
2208	46-47	-0.5899549572731573
2208	48-49	-0.32636571165530626
2208	50-51	-0.24249278524530382
2208	52-53	-0.19183725511259553
2208	54-55	-0.09330851208236624
2208	56-57	-0.04257241567129455
2208	58-59	-0.22974789759884828
2208	60-61	-0.03683958175087554
2208	62-63	-0.021219945151095487
2208	64-65	0.0031493655708487722
2208	66-67	0.20226553826254445
2208	68-69	0.1632005466278521
2208	70-71	0.22421200405084107
2208	72-73	0.35771155640261654
2208	74-75	0.43866839494219434
2208	76-77	0.4604639951684142
2208	78-79	0.5715177767198014
2208	80-81	0.6819147699314598
2208	82-83	0.7120252519641461
2208	84-85	0.7904780716706021
2208	86-87	0.9551679066200549
2208	88-89	1.0138963202425515
2208	90-91	1.2308099529960508
2208	92-93	1.3252421504388643
2208	94-95	1.4113082426354708
2208	96-97	1.4706442653919751
2208	98-99	1.4142651488064821
2208	100	1.2751875103206238
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	529343.0
3	186457.0
4	135326.0
5	95389.0
6	98056.0
7	99513.0
8	104258.0
9	119802.0
10	134039.0
11	149768.0
12	170921.0
13	220772.0
14	279852.0
15	311302.0
16	368191.0
17	379614.0
18	347051.0
19	322457.0
20	327145.0
21	345136.0
22	368775.0
23	396444.0
24	431350.0
25	473332.0
26	522210.0
27	590250.0
28	669210.0
29	766203.0
30	882075.0
31	1022781.0
32	1207875.0
33	1466843.0
34	1854697.0
35	2458134.0
36	3445076.0
37	4838606.0
38	4298554.0
39	770024.0
40	292.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.536851161254397	27.273988295526504	27.548589647073413	23.64057089614569
2	24.115083791106628	27.299906905362935	26.31202036914636	22.272988934384077
3	23.71396448712945	26.30272902725744	26.65688100506372	23.326425480549396
4	24.096202013015905	25.841814435073868	26.830873478704348	23.231110073205883
5	24.310044160059846	26.195193114841587	26.812931773061887	22.681830952036684
6	24.23922280975045	26.27068059588556	25.998836493896405	23.491260100467585
7	24.350915171936897	26.569248509814987	26.31152241320708	22.768313905041037
8	23.922230887676367	26.510004225277868	26.45651205991328	23.11125282713249
9	23.55504384466184	26.15982485183441	26.759112753060073	23.526018550443677
10-11	23.56420132327645	26.385563175841654	26.568146226706308	23.48208927417559
12-13	23.31412682349762	26.495701463734434	26.705275898750607	23.484895814017335
14-15	23.152318838423078	26.67710696126604	26.988997880205073	23.18157632010581
16-17	23.04567527200188	26.556348008110618	27.224480614043618	23.173496105843885
18-19	22.71937901542604	26.500033227844693	27.73225789676833	23.048329859960937
20-21	22.504018512784075	26.290230099206834	28.243407197731386	22.962344190277705
22-23	22.56561869414791	26.2364508394287	28.509021517704543	22.688908948718847
24-25	22.497322721044302	26.15705753882534	28.79832585440826	22.547293885722095
26-27	22.574287656372874	26.207928784238476	28.77224087017327	22.44554268921538
28-29	22.588749167861376	26.255538878791505	28.745510257430375	22.41020169591674
30-31	22.56678021926451	26.213002050478078	28.72857016960191	22.4916475606555
32-33	22.578382068232294	26.283049964975863	28.650572170138133	22.487995796653713
34-35	22.649434112870615	26.315008692636322	28.618506580370955	22.417050614122104
36-37	22.644639960178544	26.277922111707902	28.555154291774066	22.522283636339488
38-39	22.684937233933677	26.330216338001357	28.438242884191983	22.546603543872983
40-41	22.753096818029046	26.438517009226175	28.284559876651173	22.523826296093606
42-43	22.840716732298308	26.475889728527292	27.97685852946318	22.706535009711224
44-45	22.922603785339792	26.62748676928282	27.659010187771948	22.79089925760544
46-47	23.121228076784924	26.75342337351213	27.2729982164164	22.85235033328654
48-49	23.194011322079554	26.762438567445656	27.014717268397042	23.028832842077748
50-51	23.212428066234434	26.81885707041362	26.888632065337603	23.080082798014338
52-53	23.29465730770668	26.864884705547304	26.754069770145524	23.086388216600497
54-55	23.317530791606096	26.82573258227358	26.701979354030474	23.15475727208985
56-57	23.334802110715536	26.87144419308325	26.671443160223852	23.122310535977363
58-59	23.42516857850489	26.88816168141612	26.611602681040164	23.07506705903883
60-61	23.359646584884988	26.8809886336662	26.592556980579435	23.16680780086938
62-63	23.41366370365472	26.907131403839863	26.58117945806825	23.098025434437165
64-65	23.478947350615524	26.971832868147892	26.501119108203593	23.04810067303299
66-67	23.51902676120629	26.96401247680866	26.407866036126	23.109094725859052
68-69	23.539484668000142	27.035362712344437	26.340748312827134	23.084404306828294
70-71	23.648967039150023	27.09128418065918	26.23963464044865	23.020114139742144
72-73	23.622091593074675	27.11687671042855	26.141680420532488	23.119351275964288
74-75	23.717786308305406	27.20260511201654	26.074570547442484	23.005038032235568
76-77	23.522014569266307	27.3334525309114	25.972052320777966	23.172480579044326
78-79	23.608489094696434	27.36200449661686	25.874172616838198	23.15533379184851
80-81	23.643468423640076	27.422908347159698	25.754916844452513	23.178706384747713
82-83	23.68568580060229	27.5141586078339	25.661031656726273	23.139123934837535
84-85	23.677624624912262	27.52168291479019	25.57426819791812	23.22642426237942
86-87	23.734050123766345	27.610518766846475	25.43925732753457	23.216173781852614
88-89	23.847820530256065	27.673776312118193	25.254796117410212	23.223607040215533
90-91	23.849405390384526	27.653675264071325	25.164519995706968	23.33239934983718
92-93	23.914449333476114	27.72452121400456	25.02869716989108	23.332332282628247
94-95	23.980743779308693	27.779346790815346	24.882712311488213	23.357197118387745
96-97	23.96681851078068	27.804289856113545	24.730308001243316	23.49858363186246
98-99	24.033496794746608	27.809393230233415	24.589018358640125	23.568091616379853
100	24.77828900160875	27.65823039922394	23.67062145329181	23.8928591458755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3634.0
1	2544.0
2	1400.0
3	1330.0
4	1456.0
5	1660.5
6	1830.0
7	2091.0
8	2392.5
9	2755.5
10	3274.5
11	4071.0
12	4955.0
13	6717.0
14	11841.5
15	15867.5
16	21008.5
17	22981.5
18	21915.0
19	26826.0
20	34512.0
21	43127.0
22	53405.0
23	67910.0
24	86749.5
25	108859.0
26	134757.0
27	166068.5
28	202350.0
29	240207.5
30	280101.5
31	325133.5
32	376561.5
33	435462.5
34	499354.5
35	571188.0
36	656702.0
37	763174.5
38	867256.5
39	933439.0
40	981660.0
41	1049104.0
42	1153754.0
43	1247515.0
44	1316929.5
45	1371712.0
46	1368925.5
47	1313288.5
48	1259977.5
49	1253927.5
50	1233527.0
51	1163359.0
52	1067157.5
53	954232.0
54	863611.0
55	796606.0
56	723072.5
57	650201.0
58	594251.0
59	543834.5
60	487940.0
61	433107.5
62	382727.0
63	333905.5
64	289282.0
65	248671.5
66	210980.0
67	176948.5
68	146348.5
69	119829.0
70	96879.5
71	77713.5
72	62452.0
73	50421.5
74	40610.0
75	31492.5
76	23803.0
77	18180.0
78	14256.5
79	10681.0
80	7196.5
81	4611.0
82	2896.0
83	1782.0
84	1045.5
85	626.5
86	393.0
87	243.5
88	158.5
89	100.5
90	58.0
91	35.5
92	17.5
93	9.5
94	9.0
95	7.0
96	2.5
97	2.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07036558005045865
2	0.022522115938684053
3	0.004357567705107008
4	0.014932445035087076
5	0.009818154755730434
6	0.06175305109099034
7	0.0502066189305118
8	0.06394305752409415
9	0.11217450227775098
10-11	0.124512927979923
12-13	0.0975162088532501
14-15	0.12676546021895
16-17	0.16717155987745327
18-19	0.17185297919272643
20-21	0.18021219847691625
22-23	0.20203370474410226
24-25	0.13518880853485588
26-27	0.1926468177266624
28-29	0.16669059213958276
30-31	0.14388470523555508
32-33	0.178714785586346
34-35	0.46600002186799977
36-37	0.5343824116126389
38-39	0.5569894985183468
40-41	0.5431664857319478
42-43	0.5506166759915623
44-45	0.5705511855005029
46-47	0.5911766853261841
48-49	0.3444001551537793
50-51	0.019822283703437475
52-53	0.02022629660324872
54-55	0.03127733199372061
56-57	0.03698321259065801
58-59	0.025270044947717684
60-61	0.007264216067637916
62-63	0.01675691598740929
64-65	0.007206499939093451
66-67	0.01330356762949888
68-69	0.007730754773372331
70-71	0.019950541766869615
72-73	0.0033427257782001883
74-75	0.0068297418777615365
76-77	0.01155444828944305
78-79	0.005378822535185435
80-81	0.006836154780933143
82-83	0.009340393469445707
84-85	0.006688658007986181
86-87	0.00819248380172804
88-89	0.008593290249953483
90-91	0.005463793502209229
92-93	0.004990841893303208
94-95	0.010677483780725782
96-97	0.014029828913683382
98-99	0.009635387015339633
100	0.006553987041382432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.1187123E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.97325732036812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.571277918723	35.78579557149255
2	11.3473301206585	10.206527948310772
3	3.672300735897621	4.954659778599027
4	1.5363042641767939	2.76370427980807
5	0.8967377296637463	2.0164608332525185
6	0.5432557787539354	1.4659189157206627
7	0.353316892411927	1.1122868062652087
8	0.24053690347283596	0.8654182443942721
9	0.1887698494155811	0.7640635510884664
>10	1.3540831290214768	12.329111312303235
>50	0.1264491145602718	3.890890679934003
>100	0.1411755943541549	14.35349956575897
>500	0.026666992405975323	7.912478615220694
>1k	0.0016807086278841212	0.9616016374537557
>5k	3.570870508419321E-5	0.10681727912062382
>10k+	7.855915111311885E-5	0.5107649812772233
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.412903171607077E-6	0.0	0.0	0.0
3	0.0	6.412903171607077E-6	0.0	3.2064515858035384E-6	3.2064515858035384E-6
4	0.0	6.412903171607077E-6	0.0	3.2064515858035384E-6	3.2064515858035384E-6
5	0.0	6.412903171607077E-6	0.0	3.2064515858035384E-6	3.2064515858035384E-6
6	0.0	9.619354757410614E-6	0.0	6.412903171607077E-6	3.2064515858035384E-6
7	0.0	9.619354757410614E-6	0.0	9.619354757410614E-6	3.2064515858035384E-6
8	0.0	1.2825806343214154E-5	0.0	1.603225792901769E-5	3.2064515858035384E-6
9	0.0	2.5651612686428307E-5	0.0	1.603225792901769E-5	3.2064515858035384E-6
10-11	6.412903171607077E-6	2.7254838479330076E-5	0.0	1.923870951482123E-5	3.2064515858035384E-6
12-13	5.771612854446369E-5	2.8858064272231845E-5	0.0	2.0841935307722997E-5	3.2064515858035384E-6
14-15	2.0200644990562292E-4	3.366774165093715E-5	0.0	2.404838689352654E-5	3.2064515858035384E-6
16-17	4.5531612518410244E-4	4.1683870615445995E-5	0.0	2.5651612686428307E-5	3.2064515858035384E-6
18-19	8.673451539598571E-4	4.1683870615445995E-5	0.0	2.8858064272231845E-5	3.2064515858035384E-6
20-21	0.001555129019114716	4.3287096408347764E-5	0.0	2.8858064272231845E-5	3.2064515858035384E-6
22-23	0.002395219334595243	4.64935479941513E-5	0.0	2.8858064272231845E-5	3.2064515858035384E-6
24-25	0.0034389193257742946	5.1303225372856614E-5	0.0	2.8858064272231845E-5	3.2064515858035384E-6
26-27	0.004514683832811382	5.1303225372856614E-5	0.0	3.206451585803538E-5	3.2064515858035384E-6
28-29	0.0055503676950259245	5.931935433736546E-5	0.0	3.366774165093715E-5	3.2064515858035384E-6
30-31	0.006507493493388281	6.092258013026723E-5	0.0	3.687419323674069E-5	3.2064515858035384E-6
32-33	0.007459809614371931	6.73354833018743E-5	0.0	3.847741902964246E-5	3.2064515858035384E-6
34-35	0.008671848313805668	7.535161226638315E-5	0.0	3.847741902964246E-5	3.2064515858035384E-6
36-37	0.009898316045375524	8.176451543799022E-5	0.0	4.0080644822544226E-5	3.2064515858035384E-6
38-39	0.011230596679276892	8.978064440249906E-5	0.0	4.1683870615445995E-5	3.2064515858035384E-6
40-41	0.013018193438362365	9.459032178120437E-5	0.0	4.3287096408347764E-5	3.2064515858035384E-6
42-43	0.015293170838489977	1.0100322495281146E-4	0.0	4.809677378705308E-5	3.2064515858035384E-6
44-45	0.01863269016510436	1.0260645074571323E-4	0.0	5.1303225372856614E-5	3.2064515858035384E-6
46-47	0.023278838512933685	1.0581290233151677E-4	0.0	5.1303225372856614E-5	3.2064515858035384E-6
48-49	0.029707773942469783	1.0581290233151677E-4	0.0	5.1303225372856614E-5	3.2064515858035384E-6
50-51	0.03834435128883161	1.090193539173203E-4	0.0	5.1303225372856614E-5	3.2064515858035384E-6
52-53	0.050342893122908454	1.1543225708892738E-4	0.0	5.450967695866015E-5	3.2064515858035384E-6
54-55	0.06622925750477208	1.1543225708892738E-4	0.0	5.450967695866015E-5	3.2064515858035384E-6
56-57	0.08687239281417526	1.1703548288182915E-4	0.0	5.450967695866015E-5	3.2064515858035384E-6
58-59	0.11313323130190624	1.1863870867473092E-4	0.0	5.611290275156192E-5	3.2064515858035384E-6
60-61	0.1471681116594179	1.2344838605343622E-4	0.0	5.931935433736546E-5	3.2064515858035384E-6
62-63	0.19014578548973562	1.25051611846338E-4	0.0	6.412903171607076E-5	9.619354757410614E-6
64-65	0.24382499148767267	1.2665483763923976E-4	0.0	6.412903171607076E-5	9.619354757410614E-6
66-67	0.3099019425421191	1.2825806343214153E-4	0.0	6.73354833018743E-5	1.2825806343214154E-5
68-69	0.39051373863501293	1.2825806343214153E-4	0.0	7.374838647348138E-5	1.2825806343214154E-5
70-71	0.48571168299172707	1.394806439824539E-4	0.0	7.374838647348138E-5	1.603225792901769E-5
72-73	0.5976970046259156	1.4429032136115922E-4	0.0	7.374838647348138E-5	1.923870951482123E-5
74-75	0.7305435002773419	1.4749677294696275E-4	0.0	7.374838647348138E-5	1.923870951482123E-5
76-77	0.8837221054343487	1.4749677294696275E-4	1.6032257929017692E-6	7.535161226638315E-5	2.0841935307722997E-5
78-79	1.0591791362095182	1.4749677294696275E-4	3.2064515858035384E-6	7.695483805928491E-5	2.5651612686428307E-5
80-81	1.2577787313052249	1.4749677294696275E-4	3.2064515858035384E-6	7.855806385218668E-5	2.8858064272231845E-5
82-83	1.480657577808636	1.4909999873986452E-4	3.2064515858035384E-6	8.016128964508845E-5	2.8858064272231845E-5
84-85	1.729503872479677	1.5390967611856983E-4	3.2064515858035384E-6	8.657419281669553E-5	2.8858064272231845E-5
86-87	2.003645863711122	1.5390967611856983E-4	3.2064515858035384E-6	8.978064440249906E-5	2.8858064272231845E-5
88	2.2264028650542724	1.5390967611856983E-4	3.2064515858035384E-6	8.978064440249906E-5	3.206451585803538E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	18045	0.0	46.31635	1
TCTCGAG	22315	0.0	37.179348	2
CTCGAGG	28650	0.0	28.801554	3
TCGAGGG	34170	0.0	24.053091	4
GATCCGG	24765	0.0	20.677755	1
ATCCGGC	27945	0.0	18.465271	2
CGAGGGC	48090	0.0	17.185707	5
AGGGCGC	47830	0.0	17.113453	7
TATCTCG	11755	0.0	16.106369	1
GGGCGCG	51970	0.0	15.81369	8
GGATCCG	17850	0.0	15.554802	1
GAGGGCG	55260	0.0	15.008415	6
TCCGGCG	36840	0.0	14.112267	3
GCGCCGT	36170	0.0	14.108322	9
CGGCGCG	38080	0.0	13.411859	5
ATATCTC	13880	0.0	13.031261	1
CGTCGAC	33315	0.0	12.253135	12-13
GGCGCGC	85830	0.0	12.252226	9
CCGGCGC	44410	0.0	11.71755	4
CCGTCGA	33420	0.0	11.666139	12-13
>>END_MODULE