FastQCFastQC Report
Wed 25 May 2016
SRR445729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59155370
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1733310.2930097470440976No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1522340.25734603637843867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA929710.15716409178067856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG537150.067.763262
ATATCTC577750.063.76571
ATCTCGA738300.049.0347183
TATGGAT654900.045.003562
ATATGGA679300.043.7590371
TCTCGAG824600.043.600864
CTCGAGG947550.037.6336335
GGATCCG893000.032.2671665
ATGGATC922800.031.9706863
TGGATCC1003500.029.2029724
TCGAGGG1224550.029.081826
GATCCGG1164950.024.7405416
CGAGGGC1459400.024.350167
ATCCGGC1265450.022.4523937
AGGGCGC1578150.022.3749479
GAGGGCG1593100.022.3036178
TCCGGCG1414750.019.9434418
CCGGCGC2022450.013.8788619
GCGCCGT1459700.013.4989619
CGTCGAC1425800.012.34180112-13
Produced by FastQC (version 0.11.5)