##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445729_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59155370
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237443329320737	33.0	31.0	34.0	28.0	34.0
2	31.49456938567031	34.0	31.0	34.0	28.0	34.0
3	31.609735143233827	34.0	31.0	34.0	28.0	34.0
4	35.00916489914609	37.0	35.0	37.0	32.0	37.0
5	34.752471111244844	37.0	35.0	37.0	32.0	37.0
6	34.67541918848619	37.0	35.0	37.0	32.0	37.0
7	34.61149778963431	37.0	35.0	37.0	32.0	37.0
8	34.56957216225678	37.0	35.0	37.0	32.0	37.0
9	36.14266321045748	39.0	37.0	39.0	32.0	39.0
10-11	36.08660723447423	39.0	37.0	39.0	31.0	39.0
12-13	36.01491020172809	39.0	37.0	39.0	31.0	39.0
14-15	37.204578848885575	40.0	38.0	41.0	31.0	41.0
16-17	37.0999461164726	40.0	37.5	41.0	31.0	41.0
18-19	37.05699245055859	40.0	37.0	41.0	31.0	41.0
20-21	36.97235198596509	40.0	37.0	41.0	30.5	41.0
22-23	36.85205732801604	40.0	37.0	41.0	30.0	41.0
24-25	36.713808518144674	40.0	37.0	41.0	30.0	41.0
26-27	36.48060015515075	40.0	36.5	41.0	30.0	41.0
28-29	36.214805976194555	40.0	36.0	41.0	29.5	41.0
30-31	35.955149735349465	39.5	35.0	41.0	28.5	41.0
32-33	35.7377713637832	39.0	35.0	41.0	27.0	41.0
34-35	35.61123840489883	39.0	35.0	41.0	27.0	41.0
36-37	35.40732854008013	39.0	35.0	41.0	26.0	41.0
38-39	35.22302039527435	39.0	35.0	41.0	25.0	41.0
40-41	35.11091316815363	39.0	35.0	41.0	24.0	41.0
42-43	34.80511444692173	39.0	35.0	41.0	22.5	41.0
44-45	34.516871553672985	39.0	35.0	41.0	19.5	41.0
46-47	34.13454552815746	39.0	34.0	41.0	13.5	41.0
48-49	33.706651112147554	39.0	33.5	41.0	7.5	41.0
50-51	33.367634307756	39.0	33.0	41.0	2.0	41.0
52-53	33.065894296663174	38.0	33.0	41.0	2.0	41.0
54-55	32.73524814399775	38.0	33.0	40.0	2.0	41.0
56-57	32.32227082680744	38.0	32.0	40.0	2.0	41.0
58-59	31.994634950301215	37.0	31.5	40.0	2.0	41.0
60-61	31.561013928236775	37.0	31.0	40.0	2.0	41.0
62-63	31.14334221052121	36.0	30.5	40.0	2.0	41.0
64-65	30.654642055319744	35.5	30.0	39.0	2.0	41.0
66-67	30.179621292200522	35.0	29.0	39.0	2.0	41.0
68-69	29.734153272982656	35.0	29.0	39.0	2.0	40.5
70-71	29.2070981637001	35.0	28.0	38.0	2.0	40.0
72-73	28.6999150203946	35.0	26.5	37.0	2.0	40.0
74-75	28.13870460957306	34.0	26.0	36.5	2.0	39.0
76-77	27.39703672718132	33.5	25.5	36.0	2.0	39.0
78-79	27.3619847699372	34.0	25.5	36.0	2.0	38.5
80-81	26.958665265723127	34.0	24.5	35.0	2.0	37.0
82-83	26.481139675062465	34.0	22.5	35.0	2.0	37.0
84-85	25.956272693417354	33.5	19.5	35.0	2.0	36.0
86-87	25.47263433564865	33.0	16.5	35.0	2.0	36.0
88-89	24.979714352221954	33.0	6.0	35.0	2.0	36.0
90-91	24.45731524458388	33.0	2.0	35.0	2.0	35.0
92-93	23.767179632212596	32.0	2.0	35.0	2.0	35.0
94-95	23.189656644865885	32.0	2.0	35.0	2.0	35.0
96-97	22.64241034245919	31.5	2.0	35.0	2.0	35.0
98-99	21.996201528280526	31.0	2.0	35.0	2.0	35.0
100	21.056356253033325	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19206017184702162
1101	2	0.19338888457533088
1101	3	0.1823390532825222
1101	4	0.1879209306522256
1101	5	0.1876485407718036
1101	6	0.2442560486568155
1101	7	0.19830363702953946
1101	8	0.22110998463502085
1101	9	0.2797340757943303
1101	10-11	0.24840566242997397
1101	12-13	0.24503765886830564
1101	14-15	0.2942210679098807
1101	16-17	0.2841242392606844
1101	18-19	0.2619440333169223
1101	20-21	0.2878109543457086
1101	22-23	0.3079540613409364
1101	24-25	0.3219982669892616
1101	26-27	0.3111551230065501
1101	28-29	0.2845800140488919
1101	30-31	0.2890886881187882
1101	32-33	0.29010549417460396
1101	34-35	0.25442307135281794
1101	36-37	0.21739697640054345
1101	38-39	0.23378352469558905
1101	40-41	0.24737231743775112
1101	42-43	0.23520903474584998
1101	44-45	0.23262954676215486
1101	46-47	0.2536081485163919
1101	48-49	0.2750636752364386
1101	50-51	0.3172397214185452
1101	52-53	0.2930126459306237
1101	54-55	0.21467383730857392
1101	56-57	0.22414090960808153
1101	58-59	0.1803106274033084
1101	60-61	0.19406428894604133
1101	62-63	0.28692812184310057
1101	64-65	0.23653894030385203
1101	66-67	0.21649239633407902
1101	68-69	0.2002773403929332
1101	70-71	0.3144547163864466
1101	72-73	0.2709756749666319
1101	74-75	0.20296004439072846
1101	76-77	0.17207615314009317
1101	78-79	0.25913800014847865
1101	80-81	0.2565167642823134
1101	82-83	0.2054235531056463
1101	84-85	0.22489180119485397
1101	86-87	0.14625019380905613
1101	88-89	0.1040256483628248
1101	90-91	0.25121138033573587
1101	92-93	0.24977256632681488
1101	94-95	0.38866926450620554
1101	96-97	0.4190138787170383
1101	98-99	0.4395650455621656
1101	100	0.5009076811702684
1102	1	0.14434392274759134
1102	2	0.17626605155693653
1102	3	0.14635042028052325
1102	4	0.160614769739432
1102	5	0.1862690614400151
1102	6	0.2154330573004657
1102	7	0.21627115340105973
1102	8	0.19968249760621148
1102	9	0.19056704834021332
1102	10-11	0.20166301247861895
1102	12-13	0.2342073029249505
1102	14-15	0.2679986745855061
1102	16-17	0.28172594622618163
1102	18-19	0.246388318300923
1102	20-21	0.19550191763880775
1102	22-23	0.23945801130138733
1102	24-25	0.2631681823599976
1102	26-27	0.2539334081160831
1102	28-29	0.29748207943061544
1102	30-31	0.30162236377497464
1102	32-33	0.2779970190928367
1102	34-35	0.23952574081239675
1102	36-37	0.22594323443595243
1102	38-39	0.23402162414192418
1102	40-41	0.20615678574346674
1102	42-43	0.22380089434329875
1102	44-45	0.20494611982615396
1102	46-47	0.039594566336489834
1102	48-49	0.19450505753725622
1102	50-51	0.12107949980141086
1102	52-53	0.17441501048502772
1102	54-55	0.11729219541019376
1102	56-57	0.14953705489772773
1102	58-59	0.13674488094145687
1102	60-61	0.21972839739415306
1102	62-63	0.28856271353647855
1102	64-65	0.18303042578715178
1102	66-67	0.18255990787039522
1102	68-69	0.17442937770263356
1102	70-71	0.24997647497546183
1102	72-73	0.18193592589073404
1102	74-75	0.12173271558125265
1102	76-77	0.11382030465330217
1102	78-79	0.21611603534602608
1102	80-81	0.16504285033373023
1102	82-83	0.050210179468781035
1102	84-85	0.16790178539924838
1102	86-87	0.1020797104383
1102	88-89	0.012800258013015764
1102	90-91	0.144825767297192
1102	92-93	0.1942695334507114
1102	94-95	0.30175479426069884
1102	96-97	0.30730571750350677
1102	98-99	0.32575821119275616
1102	100	0.3821661306221955
1103	1	0.03809752739159222
1103	2	0.06344787376035654
1103	3	0.0506330523846259
1103	4	0.07998872811822366
1103	5	0.06105487095666717
1103	6	0.053741607863479146
1103	7	0.0544662545487995
1103	8	0.051764909034275775
1103	9	0.10757034601665794
1103	10-11	0.05787798888833606
1103	12-13	0.08478791518023598
1103	14-15	0.08490490572366127
1103	16-17	0.10213361757774209
1103	18-19	0.08735881276362534
1103	20-21	0.09498083194941387
1103	22-23	0.10224231501162961
1103	24-25	0.14800948877300613
1103	26-27	0.06161741029072232
1103	28-29	0.010541039973695376
1103	30-31	0.022658402028660873
1103	32-33	0.018513367115822632
1103	34-35	0.05777324596165556
1103	36-37	0.06166951135912058
1103	38-39	-0.011499891774164439
1103	40-41	0.08560643948796809
1103	42-43	0.007314614450187662
1103	44-45	0.02646094757312767
1103	46-47	-0.057785923578236975
1103	48-49	-0.009249655538582147
1103	50-51	-0.015025177201437145
1103	52-53	-0.013624864169464956
1103	54-55	-0.022510870931263582
1103	56-57	0.05405953662556584
1103	58-59	0.10096632137589268
1103	60-61	0.04312094776450337
1103	62-63	0.13185242403462283
1103	64-65	0.030022515838101782
1103	66-67	0.09957612926073622
1103	68-69	0.05910523481931662
1103	70-71	0.1779608926662597
1103	72-73	0.12438004827916771
1103	74-75	0.015304940300218561
1103	76-77	0.006110947163989522
1103	78-79	0.11072718861138853
1103	80-81	0.0727119465262227
1103	82-83	-0.009059442616177193
1103	84-85	0.01822973431336905
1103	86-87	-0.01802734977905729
1103	88-89	-0.0554257065904018
1103	90-91	0.045763954773892834
1103	92-93	0.044659774618676096
1103	94-95	0.1551692246558467
1103	96-97	0.10981557560966237
1103	98-99	0.08849060674595677
1103	100	0.13549868620014394
1104	1	-0.017119806707516005
1104	2	0.027673933566656927
1104	3	-0.01835452407172511
1104	4	0.02434149611927694
1104	5	-0.1643743360041583
1104	6	-0.027408380261505272
1104	7	0.018161888897367362
1104	8	0.03770567188261964
1104	9	0.014862758113629582
1104	10-11	0.00891420001488541
1104	12-13	0.010543704877399307
1104	14-15	0.052222508739035334
1104	16-17	0.08019428469594203
1104	18-19	0.02392689665114034
1104	20-21	0.05317281951400332
1104	22-23	0.04640880028757266
1104	24-25	-0.021239908549169684
1104	26-27	-0.01680359694297806
1104	28-29	0.0015718368209789446
1104	30-31	0.02987703652892293
1104	32-33	0.051072266781396536
1104	34-35	0.010660381643120331
1104	36-37	-0.0013129426203875028
1104	38-39	0.0029928279940136804
1104	40-41	0.0184188251048667
1104	42-43	-0.014111033717277621
1104	44-45	-0.006516907805512062
1104	46-47	-0.018093653852687908
1104	48-49	-0.03230381663878745
1104	50-51	0.039656611182905976
1104	52-53	0.02882932009701733
1104	54-55	-0.0394584973300951
1104	56-57	-0.01873509676571672
1104	58-59	-0.054981556244001695
1104	60-61	-0.04714020032650623
1104	62-63	-0.08959439680690906
1104	64-65	-0.2964642580069885
1104	66-67	-0.37660905816109036
1104	68-69	-0.23102431880031915
1104	70-71	-0.08571887168687908
1104	72-73	-0.10245756686051877
1104	74-75	-0.14747153295448356
1104	76-77	-0.18565587939331607
1104	78-79	-0.057448183484691384
1104	80-81	-0.01711735862345165
1104	82-83	-0.13786169954893523
1104	84-85	-0.14001417416634254
1104	86-87	-0.18696277326292687
1104	88-89	-0.20332366483009423
1104	90-91	-0.1433297425913196
1104	92-93	-0.1552774836401376
1104	94-95	-0.040988352978942544
1104	96-97	-0.026343668321217706
1104	98-99	-0.06165770742218868
1104	100	-0.045204977703246385
1105	1	-0.14149766428425892
1105	2	-0.10461665203395754
1105	3	-0.11809936473412108
1105	4	-0.13326344376827848
1105	5	-0.13279620790380875
1105	6	-0.1828949023815838
1105	7	-0.15431646247114372
1105	8	-0.17915918884778392
1105	9	-0.1802736898440287
1105	10-11	-0.14788164561575456
1105	12-13	-0.14232511518626012
1105	14-15	-0.11656374905405897
1105	16-17	-0.1308490840403067
1105	18-19	-0.20054004825997396
1105	20-21	-0.1509502130969409
1105	22-23	-0.1418739023935487
1105	24-25	-0.15724833739421484
1105	26-27	-0.1493961129986161
1105	28-29	-0.15921169654465217
1105	30-31	-0.16074616627381744
1105	32-33	-0.15100265116731038
1105	34-35	-0.17516680613724134
1105	36-37	-0.24533860269497865
1105	38-39	-0.17158439004411008
1105	40-41	-0.11934961429783897
1105	42-43	-0.14220037786302697
1105	44-45	-0.1480313288939712
1105	46-47	-0.11669312643211072
1105	48-49	-0.18786459816173817
1105	50-51	-0.17968992296217579
1105	52-53	-0.12917153387987668
1105	54-55	-0.19771348996712135
1105	56-57	-0.18387308932171464
1105	58-59	-0.18422477238419788
1105	60-61	-0.1463809803114131
1105	62-63	-0.1560531190619372
1105	64-65	-0.22084972060264718
1105	66-67	-0.1631333312058203
1105	68-69	-0.1348978815718489
1105	70-71	-0.08494369621533693
1105	72-73	-0.05354896200197956
1105	74-75	-0.15754356390191404
1105	76-77	-0.19759328077818594
1105	78-79	-0.12087746981341496
1105	80-81	-0.12402262239964656
1105	82-83	-0.18288179624159184
1105	84-85	-0.13675941961713178
1105	86-87	-0.17964097026352377
1105	88-89	-0.2164641264562306
1105	90-91	-0.18956373231409174
1105	92-93	-0.2764791416098298
1105	94-95	-0.17072935158787317
1105	96-97	-0.21028241032449202
1105	98-99	-0.38230545914387903
1105	100	-0.28171418948261007
1106	1	-0.24311589929054378
1106	2	-0.18511634438039692
1106	3	-0.21326986569466655
1106	4	-0.1641129365900369
1106	5	-0.22435926269067608
1106	6	-0.34265612460606576
1106	7	-0.325691089048469
1106	8	-0.2986643530772142
1106	9	-0.3399414757879313
1106	10-11	-0.3496064793111202
1106	12-13	-0.33291409925261206
1106	14-15	-0.4089411021112923
1106	16-17	-0.37798666670127545
1106	18-19	-0.39244496017857244
1106	20-21	-0.3739943254370104
1106	22-23	-0.3385596843830925
1106	24-25	-0.36441735502653216
1106	26-27	-0.3204340156980905
1106	28-29	-0.34009569197470313
1106	30-31	-0.33056392379982213
1106	32-33	-0.34962657425858623
1106	34-35	-0.3405762854893055
1106	36-37	-0.35539498424066807
1106	38-39	-0.36569716613410463
1106	40-41	-0.3367643332760295
1106	42-43	-0.42611599174161796
1106	44-45	-0.3864694484554718
1106	46-47	-0.34641121022484356
1106	48-49	-0.39438302492436605
1106	50-51	-0.4265408611050958
1106	52-53	-0.3824926683661758
1106	54-55	-0.39658061575998005
1106	56-57	-0.39955299782696585
1106	58-59	-0.3936152305483631
1106	60-61	-0.3877143276302064
1106	62-63	-0.32805278333019317
1106	64-65	-0.3776636496311703
1106	66-67	-0.3949062308878162
1106	68-69	-0.48013025507590257
1106	70-71	-0.3547593943668552
1106	72-73	-0.4358374825162059
1106	74-75	-0.5664632792311082
1106	76-77	-0.6277187274030922
1106	78-79	-0.4685017688020068
1106	80-81	-0.48350735829768965
1106	82-83	-0.47169516491539554
1106	84-85	-0.45447992073757604
1106	86-87	-0.39115222021804286
1106	88-89	-0.4739354979761856
1106	90-91	-0.3815034238354258
1106	92-93	-0.3287236006769838
1106	94-95	-0.2799144278795289
1106	96-97	-0.26868377015755485
1106	98-99	-0.2974949743013262
1106	100	-0.3483018780632321
1107	1	-0.19870045174232231
1107	2	-0.18445071575078842
1107	3	-0.20257654413272164
1107	4	-0.17912797156201776
1107	5	-0.1750393432731272
1107	6	-0.16520112760200334
1107	7	-0.17827844901263035
1107	8	-0.20470485955799944
1107	9	-0.20966964178219172
1107	10-11	-0.21124966196178718
1107	12-13	-0.21106124989729125
1107	14-15	-0.2765871892257721
1107	16-17	-0.26079441698117023
1107	18-19	-0.2184853111560372
1107	20-21	-0.27468283963466433
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2207	66-67	0.12327553648875877
2207	68-69	0.11537009659469177
2207	70-71	0.0848905061296783
2207	72-73	0.03779294307530634
2207	74-75	0.16921008661779524
2207	76-77	0.16192460913599405
2207	78-79	0.0197525283081319
2207	80-81	-0.013704066653591696
2207	82-83	-0.07634604253448529
2207	84-85	0.010206878513461248
2207	86-87	-0.0274791318276435
2207	88-89	0.09946541223835936
2207	90-91	0.05976182908982963
2207	92-93	0.0919527771592108
2207	94-95	0.0699140558184439
2207	96-97	0.1076270943018578
2207	98-99	0.21993829186757807
2207	100	0.15297363046412826
2208	1	-0.5133784596315216
2208	2	-0.9657707810116101
2208	3	-0.6163488073476273
2208	4	-0.6015078097869591
2208	5	-0.6475960712954887
2208	6	-0.6895393672478534
2208	7	-0.6079433572292317
2208	8	-0.6534493253314153
2208	9	-0.6417864908286504
2208	10-11	-0.6489820890410556
2208	12-13	-0.7592324361122849
2208	14-15	-0.7392713126123098
2208	16-17	-1.1587302622999474
2208	18-19	-0.9026242501186701
2208	20-21	-0.8103598946311621
2208	22-23	-0.7840021603436966
2208	24-25	-0.7752734490404407
2208	26-27	-0.758062117885558
2208	28-29	-0.7598689981818936
2208	30-31	-0.7843455772697467
2208	32-33	-0.7200223701020789
2208	34-35	-0.7250684290664893
2208	36-37	-0.7466768201360665
2208	38-39	-0.6691566590098006
2208	40-41	-0.7302913865201432
2208	42-43	-0.734110216711521
2208	44-45	-0.7278591319449461
2208	46-47	-0.5912828392473415
2208	48-49	-0.5562521237887097
2208	50-51	-0.6087855845775181
2208	52-53	-0.6042984850547484
2208	54-55	-0.5335544928328702
2208	56-57	-0.5794180817393908
2208	58-59	-0.6585391921384343
2208	60-61	-1.0226525934120794
2208	62-63	-0.6869374441476346
2208	64-65	-1.042440600314972
2208	66-67	-1.0863907803286246
2208	68-69	-0.6383460273390789
2208	70-71	-1.3280594709242841
2208	72-73	-0.7992831510313358
2208	74-75	-0.40492099837987894
2208	76-77	-0.26756838151404594
2208	78-79	-0.3515925062090801
2208	80-81	-0.27430475057186143
2208	82-83	-0.20877431559792825
2208	84-85	-0.1797923286906773
2208	86-87	-0.13524351350580943
2208	88-89	-8.325736001388861E-4
2208	90-91	-0.17205063168015755
2208	92-93	-0.8276070614640609
2208	94-95	-0.6213062456803549
2208	96-97	-0.36218862526621365
2208	98-99	0.02531413783429315
2208	100	-0.08812740245145889
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1092160.0
3	351488.0
4	211837.0
5	172675.0
6	167740.0
7	187738.0
8	213170.0
9	234684.0
10	255035.0
11	263713.0
12	282163.0
13	343644.0
14	485569.0
15	705199.0
16	809720.0
17	780426.0
18	758738.0
19	616838.0
20	540764.0
21	547074.0
22	579733.0
23	631646.0
24	700563.0
25	786836.0
26	890125.0
27	1007875.0
28	1147207.0
29	1304327.0
30	1506180.0
31	1738564.0
32	2054913.0
33	2488842.0
34	3160045.0
35	4217118.0
36	6091237.0
37	9176719.0
38	1.0053813E7
39	2595695.0
40	3557.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.637969788352105	28.177076051193676	27.456617397065802	22.728336763388416
2	23.363555359646014	27.070340563458334	28.42568319775725	21.1404208791384
3	23.258119787808706	27.109872585892642	27.076577176463406	22.55543044983525
4	23.812010041219647	26.134663924405526	27.502278486774667	22.551047547600163
5	24.559438632241502	26.212456241336145	26.144798486449666	23.083306639972687
6	24.704073119696883	26.04592521940468	26.124904532149763	23.12509712874867
7	24.113374060172937	26.663640255123315	25.743144685924374	23.479840998779373
8	24.248902211264998	25.95850784579162	26.26058400873092	23.532005934212467
9	24.09767152616045	26.245815500710535	25.88987219948729	23.76664077364173
10-11	24.556602733779236	25.94252041341066	25.840788920718776	23.660087932091326
12-13	23.97423645350384	26.081883212891157	26.049573336956566	23.89430699664844
14-15	23.54541327194957	26.213383378350734	26.576222545706113	23.664980803993586
16-17	23.326625307981317	26.271810679394886	26.916677575198904	23.484886437424894
18-19	22.87632016220975	26.271144400536556	27.803979917308265	23.04855551994543
20-21	22.309298909837548	26.356743295087163	28.90945465336837	22.42450314170692
22-23	22.07782486508318	26.238906404335687	29.477495786912083	22.205772943669043
24-25	21.800441530039606	25.930219888424098	30.204826293699018	22.064512287837278
26-27	21.770995056711122	25.994373676774153	30.774368237180223	21.460263029334502
28-29	21.768193115491393	26.111993393657624	30.734628484103908	21.385185006747072
30-31	21.722886186914184	26.156941190718243	30.66390434229223	21.456268280075342
32-33	21.721093711712648	26.265818649486334	30.581233848362793	21.43185379043823
34-35	21.718813596408797	26.387926715433707	30.540735203425207	21.35252448473229
36-37	21.72624058452835	26.472264029599945	30.396363580700523	21.40513180517118
38-39	21.749689143648705	26.652196847680308	30.18026756480704	21.417846443863947
40-41	21.79681381815521	26.924594771526017	29.80842704413314	21.470164366185628
42-43	21.920191408200996	27.231842187707585	29.22193496449375	21.62603143959767
44-45	22.059924620016886	27.619240216524936	28.620123596041857	21.700711567416324
46-47	22.182520576587372	27.993768784176677	28.01776536384627	21.805945275389682
48-49	22.361222918023525	28.28931827457885	27.365978264795395	21.98348054260223
50-51	22.401168503734283	28.633906598393782	26.856626990615602	22.108297907256336
52-53	22.46200420456633	28.837648960467142	26.58686049862374	22.11348633634279
54-55	22.49455575247904	28.946591313932952	26.41507918870138	22.14377374488663
56-57	22.46126871417145	29.163799568077398	26.26502774786193	22.109903969889224
58-59	22.474608454369438	29.34591050462876	26.135050358729444	22.044430682272363
60-61	22.43728505433226	29.50701848524817	25.97135628721824	22.084340173201326
62-63	22.441801223896913	29.712424067129405	25.876214835264378	21.9695598737093
64-65	22.43007140973453	29.93042333049148	25.71004894631899	21.929456313455
66-67	22.43679585604046	30.107326121063306	25.522536262414143	21.93334176048209
68-69	22.43219438486602	30.321749698431105	25.34683560694916	21.899220309753716
70-71	22.406382555389072	30.540018652820468	25.17782006417614	21.875778727614325
72-73	22.38071829353803	30.71630257343606	25.00892848939494	21.894050643630973
74-75	22.412584600404948	30.903602504244258	24.862532279668926	21.821280615681868
76-77	22.15620902481757	31.3035257718015	24.598649713839926	21.941615489541004
78-79	22.17560886566877	31.483058072105614	24.39705692372955	21.94427613849606
80-81	22.14980524010689	31.686521493591215	24.235504089764195	21.9281691765377
82-83	22.11897220633797	31.921689995520662	24.06880130007822	21.890536498063145
84-85	22.07146191610783	32.106243501696774	23.828490691340434	21.993803890854963
86-87	22.077185809976164	32.29890206912258	23.637268179071427	21.986643941829826
88-89	22.08656605759638	32.47759480209349	23.39533651895024	22.040502621359888
90-91	22.11256836864699	32.5961269262917	23.167200395199185	22.124104309862126
92-93	22.067850781304543	32.80302874234427	22.91147373045445	22.217646745896733
94-95	22.1140237485219	32.93447163097463	22.685091616370087	22.26641300413338
96-97	22.104918179756137	33.07328019363857	22.443731226893235	22.378070399712062
98-99	22.117036740699508	33.18885873723428	22.261230091693232	22.432874430372983
100	22.567256616610173	32.96132067199927	21.544994457310267	22.926428254080292
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13633.0
1	9129.5
2	4089.5
3	3509.5
4	3665.0
5	4183.5
6	4971.0
7	5916.5
8	7122.0
9	8661.5
10	10636.0
11	13418.0
12	17467.0
13	24477.0
14	37053.5
15	51723.5
16	88522.0
17	104336.5
18	98705.0
19	120378.5
20	154165.5
21	186655.5
22	218179.0
23	261699.5
24	311915.5
25	370711.0
26	438341.5
27	512635.0
28	599872.0
29	692403.0
30	773891.5
31	858253.5
32	957649.0
33	1066772.5
34	1182394.0
35	1327138.0
36	1494010.5
37	1636662.0
38	1767114.0
39	1878420.5
40	1954271.5
41	2022351.5
42	2112668.0
43	2230105.5
44	2345460.5
45	2391901.5
46	2365021.5
47	2300105.5
48	2208753.5
49	2119408.0
50	2022303.5
51	1925601.0
52	1805734.0
53	1653951.5
54	1510626.0
55	1383161.0
56	1251804.0
57	1118916.0
58	1012412.0
59	911781.5
60	799327.0
61	702281.5
62	611699.5
63	515517.5
64	432576.5
65	363335.5
66	307034.5
67	255697.0
68	207705.5
69	169669.5
70	141225.0
71	121809.0
72	108429.5
73	97234.5
74	84399.0
75	66880.5
76	51512.5
77	43438.0
78	38540.0
79	30623.0
80	19778.5
81	11121.0
82	6344.0
83	3519.0
84	2076.0
85	1278.0
86	819.5
87	561.5
88	360.0
89	200.5
90	113.5
91	74.5
92	61.0
93	46.0
94	27.0
95	14.5
96	14.0
97	11.5
98	6.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011258487606450606
2	0.01333944830367894
3	1.1495152511090709E-4
4	0.027620484835104573
5	0.04046462730264387
6	0.0010802062433216122
7	0.0010210400171615864
8	1.521417244114947E-4
9	7.488753768254683E-4
10-11	0.0010649920708804626
12-13	1.3946324737720345E-4
14-15	1.6397496964349982E-4
16-17	0.0010218852489638725
18-19	0.0014360488320840526
20-21	7.184470319431693E-4
22-23	2.1046271876923428E-4
24-25	6.618165011900019E-4
26-27	3.702115294013037E-4
28-29	1.605940424343555E-4
30-31	1.960937781303709E-4
32-33	0.0025982425602274145
34-35	0.0030825603829373396
36-37	0.0013616684334828773
38-39	0.0024334223587816287
40-41	0.004756964583266067
42-43	0.006561534481146851
44-45	0.006969781441651029
46-47	0.0053925788985852005
48-49	0.002416517722735907
50-51	0.0018383791699722273
52-53	0.002684456204060595
54-55	0.0035634972784381195
56-57	0.011120714822677975
58-59	0.01306728366334282
60-61	0.015015542967612238
62-63	0.015434777941546136
64-65	0.014423035474209697
66-67	0.002227185799023825
68-69	2.4934338167439404E-4
70-71	0.004032600928706895
72-73	0.0010590754482644603
74-75	0.005734897778511063
76-77	4.724845774779196E-4
78-79	8.993266376323907E-4
80-81	0.0012458716765696843
82-83	0.004431550339385926
84-85	8.536841203089423E-4
86-87	7.040780913043059E-4
88-89	0.005490625787650386
90-91	0.00921302664491829
92-93	0.013380864661990958
94-95	0.019332987013689542
96-97	0.025501488706773368
98-99	0.02299030502218142
100	0.01520741058673118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.915537E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.697823192951354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96152118372991	29.00847693042658
2	12.161965209497316	9.412831587119054
3	4.609759462991797	5.351629699826728
4	2.2109717434816547	3.4223917445545786
5	1.299975379992561	2.5153108705070943
6	0.8777302480998092	2.037974997124304
7	0.5949673784675402	1.611675969225745
8	0.4453794542198854	1.3788172298539438
9	0.3650748102271089	1.2714840412532036
>10	2.0582266941657994	14.737844565386968
>50	0.2021821748640541	5.480369533546578
>100	0.19223797065616646	14.561253144334586
>500	0.013530820546340638	3.575612210503353
>1k	0.006315674672856922	3.8795087013381737
>5k	3.935604091255255E-5	0.10965825531544172
>10k+	1.2243834675648203E-4	1.645160519683768
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	173331	0.2930097470440976	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	152234	0.25734603637843867	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92971	0.15716409178067856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	5.071390813716489E-6	0.0	3.3809272091443264E-6	1.6904636045721632E-6
2	0.0	6.761854418288653E-6	0.0	3.3809272091443264E-6	6.761854418288653E-6
3	0.0	6.761854418288653E-6	0.0	1.0142781627432978E-5	1.0142781627432978E-5
4	1.6904636045721632E-6	6.761854418288653E-6	0.0	1.1833245232005142E-5	1.3523708836577306E-5
5	5.071390813716489E-6	1.0142781627432978E-5	0.0	1.1833245232005142E-5	1.8595099650293795E-5
6	5.071390813716489E-6	1.3523708836577306E-5	0.0	2.0285563254865956E-5	2.0285563254865956E-5
7	5.071390813716489E-6	1.3523708836577306E-5	0.0	2.5356954068582447E-5	2.197602685943812E-5
8	6.761854418288653E-6	1.3523708836577306E-5	0.0	3.21188084868711E-5	2.704741767315461E-5
9	1.1833245232005142E-5	1.3523708836577306E-5	0.0	3.380927209144326E-5	2.873788127772677E-5
10-11	2.7047417673154608E-5	1.5214172441149468E-5	0.0	4.395205371887624E-5	3.8035431102873666E-5
12-13	9.889212086747155E-5	1.7749867848007713E-5	0.0	5.4940067148595304E-5	4.56425173234484E-5
14-15	3.4147364812357696E-4	2.0285563254865956E-5	8.452318022860816E-7	6.254715336917003E-5	5.1559139939450976E-5
16-17	9.339811415261202E-4	2.4511722266296365E-5	2.535695406858245E-6	7.268993499660302E-5	5.4094835346309215E-5
18-19	0.0019161404957825467	2.5356954068582447E-5	3.3809272091443264E-6	8.452318022860815E-5	6.170192156688395E-5
20-21	0.003443474362513496	2.620218587086853E-5	3.3809272091443264E-6	9.043980284461072E-5	6.592808057831436E-5
22-23	0.005391733666782915	3.21188084868711E-5	3.3809272091443264E-6	9.720165726289937E-5	7.184470319431693E-5
24-25	0.00777190642202052	4.057112650973192E-5	5.071390813716489E-6	1.0649920708804628E-4	8.114225301946382E-5
26-27	0.01064315885438634	4.56425173234484E-5	5.071390813716489E-6	1.1664198871547925E-4	8.452318022860815E-5
28-29	0.015726382913334835	4.733298092802057E-5	5.071390813716489E-6	1.284752339474844E-4	8.536841203089424E-5
30-31	0.0198274476180269	4.817821273030665E-5	5.071390813716489E-6	1.3946324737720345E-4	9.21302664491829E-5
32-33	0.02147057824167104	5.1559139939450976E-5	5.071390813716489E-6	1.4791556540006426E-4	9.889212086747155E-5
34-35	0.023019042903459144	5.409483534630922E-5	5.071390813716489E-6	1.5805834702749727E-4	1.0818967069261844E-4
36-37	0.024725565912274743	5.663053075316746E-5	5.071390813716489E-6	1.6651066505035808E-4	1.1748722051776534E-4
38-39	0.026605361440558986	5.8320994357739625E-5	5.071390813716489E-6	1.7158205586407455E-4	1.2002291592462358E-4
40-41	0.028627155911627296	6.254715336917003E-5	5.071390813716489E-6	1.7665344667779103E-4	1.2086814772690967E-4
42-43	0.030825603829373393	6.42376169737422E-5	5.071390813716489E-6	1.8595099650293794E-4	1.234038431337679E-4
44-45	0.033567535795989444	6.592808057831436E-5	5.071390813716489E-6	1.9440331452579875E-4	1.2678477034291222E-4
46-47	0.03705411698041953	6.930900778745869E-5	5.071390813716489E-6	2.0285563254865955E-4	1.2932046574977047E-4
48-49	0.041544833545965484	7.015423958974478E-5	5.071390813716489E-6	2.1215318237380646E-4	1.377727837726313E-4
50-51	0.04697122171664213	7.099947139203085E-5	5.071390813716489E-6	2.146888777806647E-4	1.4707033359777819E-4
52-53	0.053817599315159385	7.268993499660302E-5	5.071390813716489E-6	2.163793413852369E-4	1.5721311522521117E-4
54-55	0.06238824979034025	7.607086220574735E-5	5.071390813716489E-6	2.1722457318752296E-4	1.6651066505035808E-4
56-57	0.07366364203283657	8.283271662403599E-5	5.071390813716489E-6	2.206055003966673E-4	1.7073682406178848E-4
58-59	0.08764124034724152	8.367794842632206E-5	5.071390813716489E-6	2.2736735481495592E-4	1.800343738869354E-4
60-61	0.10534123951891435	8.536841203089424E-5	5.071390813716489E-6	2.2821258661724202E-4	1.884866919097962E-4
62-63	0.12729782604689988	8.874933924003857E-5	5.916622616002571E-6	2.3328397743095853E-4	1.9524854632808485E-4
64-65	0.1542843870302899	9.63564254606133E-5	6.761854418288653E-6	2.451172226629637E-4	1.9778424173494307E-4
66-67	0.18815536104330005	1.0396351168118803E-4	6.761854418288653E-6	2.476529180698219E-4	1.9862947353722915E-4
68-69	0.2292412337206242	1.0988013429719061E-4	6.761854418288653E-6	2.5441477248811054E-4	1.9947470533951525E-4
70-71	0.2793795390004322	1.1495152511090709E-4	6.761854418288653E-6	2.654027859178296E-4	2.062365597578039E-4
72-73	0.3397214825974379	1.1917768412233749E-4	8.452318022860816E-6	2.738551039406904E-4	2.0792702336237606E-4
74-75	0.41231844209578944	1.2593953854062614E-4	9.297549825146898E-6	2.789264947544069E-4	2.1299841417609256E-4
76-77	0.49832500413740966	1.3185616115662872E-4	1.1833245232005142E-5	2.8146219016126516E-4	2.1976026859438122E-4
78-79	0.5996818209403474	1.3861801557491738E-4	1.1833245232005142E-5	2.932954353932703E-4	2.2229596400123944E-4
80-81	0.7172670883471779	1.3861801557491738E-4	1.1833245232005142E-5	3.000572898115589E-4	2.2567689121038377E-4
82-83	0.8516935994145587	1.3861801557491738E-4	1.3523708836577306E-5	3.1020007143899193E-4	2.3328397743095853E-4
84-85	1.0054378833231876	1.4030847917948953E-4	1.3523708836577306E-5	3.169619258572806E-4	2.3751013644238893E-4
86-87	1.1786317962342219	1.419989427840617E-4	1.3523708836577306E-5	3.2794993928699965E-4	2.4089106365153323E-4
88	1.3216822073803274	1.419989427840617E-4	1.3523708836577306E-5	3.380927209144326E-4	2.4173629545381933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	53715	0.0	67.76326	2
ATATCTC	57775	0.0	63.7657	1
ATCTCGA	73830	0.0	49.034718	3
TATGGAT	65490	0.0	45.00356	2
ATATGGA	67930	0.0	43.759037	1
TCTCGAG	82460	0.0	43.60086	4
CTCGAGG	94755	0.0	37.633633	5
GGATCCG	89300	0.0	32.267166	5
ATGGATC	92280	0.0	31.970686	3
TGGATCC	100350	0.0	29.202972	4
TCGAGGG	122455	0.0	29.08182	6
GATCCGG	116495	0.0	24.740541	6
CGAGGGC	145940	0.0	24.35016	7
ATCCGGC	126545	0.0	22.452393	7
AGGGCGC	157815	0.0	22.374947	9
GAGGGCG	159310	0.0	22.303617	8
TCCGGCG	141475	0.0	19.943441	8
CCGGCGC	202245	0.0	13.878861	9
GCGCCGT	145970	0.0	13.498961	9
CGTCGAC	142580	0.0	12.341801	12-13
>>END_MODULE