FastQCFastQC Report
Wed 25 May 2016
SRR445728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48727188
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1368230.28079395839546495No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1234170.25328159712397114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA799090.163992635897643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG414600.068.071052
ATATCTC457450.062.2775231
ATCTCGA578300.048.5282023
TATGGAT533550.044.6551482
ATATGGA553200.043.515971
TCTCGAG651500.042.7295384
CTCGAGG756350.036.611115
ATGGATC750500.031.7553883
GGATCCG767600.030.3295575
TGGATCC813850.029.1739084
TCGAGGG991700.027.8834786
GATCCGG984800.023.6980886
CGAGGGC1188200.023.2681647
ATCCGGC1049800.021.9218067
AGGGCGC1262850.021.7215449
GAGGGCG1270550.021.7156648
TCCGGCG1157950.019.7485458
CCGGCGC1635650.013.9693899
GCGCCGT1188500.013.001269
CCGTCGA1172900.011.96812616-17
Produced by FastQC (version 0.11.5)