##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445728_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48727188
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291429232485157	33.0	31.0	34.0	28.0	34.0
2	31.54713629278176	34.0	31.0	34.0	28.0	34.0
3	31.66049216712444	34.0	31.0	34.0	28.0	34.0
4	35.065141128193154	37.0	35.0	37.0	32.0	37.0
5	34.82063157430714	37.0	35.0	37.0	32.0	37.0
6	34.748144444534745	37.0	35.0	37.0	32.0	37.0
7	34.68684743310039	37.0	35.0	37.0	32.0	37.0
8	34.64808309890569	37.0	35.0	37.0	32.0	37.0
9	36.22733776469925	39.0	37.0	39.0	32.0	39.0
10-11	36.17215091500868	39.0	37.0	39.0	32.0	39.0
12-13	36.10064164794406	39.0	37.0	39.0	32.0	39.0
14-15	37.2957468528658	40.0	38.0	41.0	31.0	41.0
16-17	37.18909452562705	40.0	37.5	41.0	31.0	41.0
18-19	37.143230930953784	40.0	37.5	41.0	31.0	41.0
20-21	37.05848384889356	40.0	37.0	41.0	31.0	41.0
22-23	36.937570397044055	40.0	37.0	41.0	30.5	41.0
24-25	36.79716725085798	40.0	37.0	41.0	30.0	41.0
26-27	36.56315570683044	40.0	36.5	41.0	30.0	41.0
28-29	36.29842315136264	40.0	36.0	41.0	30.0	41.0
30-31	36.039961048029284	39.5	35.0	41.0	29.0	41.0
32-33	35.826283306149335	39.0	35.0	41.0	28.0	41.0
34-35	35.70168678726135	39.0	35.0	41.0	27.0	41.0
36-37	35.497345701541406	39.0	35.0	41.0	26.5	41.0
38-39	35.31247497803486	39.0	35.0	41.0	25.0	41.0
40-41	35.20226741793513	39.0	35.0	41.0	24.5	41.0
42-43	34.90041031097465	39.0	35.0	41.0	23.5	41.0
44-45	34.61683408654733	39.0	35.0	41.0	21.0	41.0
46-47	34.23925025183066	39.0	34.0	41.0	16.5	41.0
48-49	33.8178938316736	39.0	33.5	41.0	8.5	41.0
50-51	33.482325226729685	39.0	33.0	41.0	4.5	41.0
52-53	33.18099131844013	38.0	33.0	41.0	2.0	41.0
54-55	32.852416273231285	38.0	33.0	40.0	2.0	41.0
56-57	32.44058522318177	38.0	32.0	40.0	2.0	41.0
58-59	32.11381220069585	37.0	32.0	40.0	2.0	41.0
60-61	31.683891537102447	37.0	31.0	40.0	2.0	41.0
62-63	31.267721010291012	36.0	30.5	40.0	2.0	41.0
64-65	30.78211026665442	35.5	30.0	39.0	2.0	41.0
66-67	30.30778389058691	35.0	29.0	39.0	2.0	41.0
68-69	29.86464893069553	35.0	29.0	39.0	2.0	40.5
70-71	29.338191237302674	35.0	28.0	38.0	2.0	40.0
72-73	28.83151802234104	35.0	27.5	37.0	2.0	40.0
74-75	28.27134599887028	34.0	26.0	36.5	2.0	39.0
76-77	27.533145971813518	33.5	26.0	36.0	2.0	39.0
78-79	27.502617912201295	34.0	25.5	36.0	2.0	38.5
80-81	27.10167121689846	34.0	25.0	35.0	2.0	37.0
82-83	26.62384139630631	34.0	23.5	35.0	2.0	37.0
84-85	26.09838158524559	33.5	20.5	35.0	2.0	36.5
86-87	25.613641105249087	33.0	18.5	35.0	2.0	36.0
88-89	25.1196668480028	33.0	9.5	35.0	2.0	36.0
90-91	24.59550318191971	33.0	2.0	35.0	2.0	35.0
92-93	23.90336910063433	32.0	2.0	35.0	2.0	35.0
94-95	23.32504033477163	32.0	2.0	35.0	2.0	35.0
96-97	22.77503343718501	31.5	2.0	35.0	2.0	35.0
98-99	22.125213925745108	31.0	2.0	35.0	2.0	35.0
100	21.175723396966802	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18603117443605655
1101	2	0.18124803301181203
1101	3	0.19123063434659215
1101	4	0.1898732923989357
1101	5	0.19473645802774087
1101	6	0.24335375270230486
1101	7	0.20313937955804562
1101	8	0.23689080597466017
1101	9	0.2863208446257204
1101	10-11	0.24737812597604858
1101	12-13	0.25015423806166126
1101	14-15	0.3024433477210877
1101	16-17	0.2916938038884638
1101	18-19	0.2694104314390131
1101	20-21	0.28678409869093
1101	22-23	0.29952214650193554
1101	24-25	0.3202606385744744
1101	26-27	0.30276982896505444
1101	28-29	0.2740851011071044
1101	30-31	0.29102672955876585
1101	32-33	0.28611799120915293
1101	34-35	0.24621783135143716
1101	36-37	0.20825832796005272
1101	38-39	0.24500358771418718
1101	40-41	0.2559324028470442
1101	42-43	0.2289157534971764
1101	44-45	0.24836282631860485
1101	46-47	0.2562668569245332
1101	48-49	0.2776586112539903
1101	50-51	0.31978944023297373
1101	52-53	0.29793342728784467
1101	54-55	0.22205603398667506
1101	56-57	0.2350793762167882
1101	58-59	0.22101437348445074
1101	60-61	0.21605627895837998
1101	62-63	0.3167851976625329
1101	64-65	0.25571636945557685
1101	66-67	0.2501940990187492
1101	68-69	0.22846762442224744
1101	70-71	0.329465920968552
1101	72-73	0.28579641139980083
1101	74-75	0.23220763202203898
1101	76-77	0.2121839681070945
1101	78-79	0.30703883249872277
1101	80-81	0.2988749779540463
1101	82-83	0.2535236481354701
1101	84-85	0.2575270868311179
1101	86-87	0.1599512538994361
1101	88-89	0.09697044973664148
1101	90-91	0.23818724377940015
1101	92-93	0.2403986615041731
1101	94-95	0.3757443365849653
1101	96-97	0.4167994057354143
1101	98-99	0.4371214576719673
1101	100	0.4936731252802957
1102	1	0.13761091477121568
1102	2	0.15741586375633432
1102	3	0.13527210882017826
1102	4	0.14476391219884022
1102	5	0.18038277396631486
1102	6	0.2047310329402663
1102	7	0.18237272393861303
1102	8	0.16659371447445182
1102	9	0.1644329390183401
1102	10-11	0.17426165758055134
1102	12-13	0.20736484870714378
1102	14-15	0.24519537806835245
1102	16-17	0.25369035369959647
1102	18-19	0.22874429802219964
1102	20-21	0.19610327807865247
1102	22-23	0.24380505501496685
1102	24-25	0.25554031572926306
1102	26-27	0.24126274923916924
1102	28-29	0.2882763642966495
1102	30-31	0.2833166570741241
1102	32-33	0.26813186998209204
1102	34-35	0.24488835649873408
1102	36-37	0.20386904314417365
1102	38-39	0.1972801281705827
1102	40-41	0.16905116162368472
1102	42-43	0.19088801951551204
1102	44-45	0.18347257450123067
1102	46-47	0.02738026745394251
1102	48-49	0.16740290500234067
1102	50-51	0.09451804097653849
1102	52-53	0.1564802965368841
1102	54-55	0.0996413178117237
1102	56-57	0.13022849756621468
1102	58-59	0.09804524235708811
1102	60-61	0.1749926465555589
1102	62-63	0.2576006066424803
1102	64-65	0.14091932358612524
1102	66-67	0.1558019055524973
1102	68-69	0.1598547662640435
1102	70-71	0.24578499397190967
1102	72-73	0.17563459557936412
1102	74-75	0.10752517704362674
1102	76-77	0.10671806158951824
1102	78-79	0.1817959258219588
1102	80-81	0.13957442506114148
1102	82-83	0.05333945409821084
1102	84-85	0.15718016200506213
1102	86-87	0.107794575562707
1102	88-89	0.030466403335086767
1102	90-91	0.15344514186086755
1102	92-93	0.1610796157086689
1102	94-95	0.2826089538449139
1102	96-97	0.3108432445771676
1102	98-99	0.292673470336446
1102	100	0.3593020081169662
1103	1	0.045571014808651
1103	2	0.07385401560684457
1103	3	0.05991907584234113
1103	4	0.08298349469095712
1103	5	0.05848393914872929
1103	6	0.04156269507389965
1103	7	0.047528443272959464
1103	8	0.04318781494998092
1103	9	0.09694695240088436
1103	10-11	0.0446286234814508
1103	12-13	0.0708805150548315
1103	14-15	0.06266106850903697
1103	16-17	0.09043224720753074
1103	18-19	0.07947518160993639
1103	20-21	0.07685666185902562
1103	22-23	0.10163938402835981
1103	24-25	0.11959790276016236
1103	26-27	0.038880474829973366
1103	28-29	0.02283219054261565
1103	30-31	0.01076090591837442
1103	32-33	0.001869350426652261
1103	34-35	0.046922267356784175
1103	36-37	0.061847559955054976
1103	38-39	0.01317455564996095
1103	40-41	0.10422924363346198
1103	42-43	0.03217190801502312
1103	44-45	0.05268949667306089
1103	46-47	-0.028496887283161243
1103	48-49	0.018238696283937372
1103	50-51	-0.003166099858233906
1103	52-53	-0.003410992311913219
1103	54-55	-0.0067097250813645815
1103	56-57	0.0796036641203628
1103	58-59	0.11752761723997196
1103	60-61	0.05209663217378235
1103	62-63	0.12532821464471766
1103	64-65	0.014711923491567802
1103	66-67	0.07475081236750825
1103	68-69	0.028943429472722215
1103	70-71	0.15312547119505027
1103	72-73	0.06255607593347179
1103	74-75	-0.022315401954415393
1103	76-77	-0.03251322123209732
1103	78-79	0.09350246094559722
1103	80-81	0.058746040005718925
1103	82-83	-0.04741340051935694
1103	84-85	-0.017561261221011648
1103	86-87	-0.0683266102218596
1103	88-89	-0.09022462578245793
1103	90-91	-0.0227381014498782
1103	92-93	-3.0769184879275713E-4
1103	94-95	0.123096760002813
1103	96-97	0.08507452152550243
1103	98-99	0.046556828944911643
1103	100	0.10076047882919426
1104	1	-0.05262245796744125
1104	2	-0.010222643669269615
1104	3	-0.0528516168916795
1104	4	-0.019797764521257477
1104	5	-0.2261698925972695
1104	6	-0.07221457428462941
1104	7	-0.03835211252212645
1104	8	-0.019377624295103146
1104	9	-0.044383620485007214
1104	10-11	-0.0669181044738636
1104	12-13	-0.06313163517175724
1104	14-15	-0.031132930406364778
1104	16-17	0.006822794888279304
1104	18-19	-0.04356702159451231
1104	20-21	-0.03438151624189345
1104	22-23	-0.05913948877630304
1104	24-25	-0.1016077448607291
1104	26-27	-0.08491200493740791
1104	28-29	-0.047279548959608064
1104	30-31	-0.052626987781728474
1104	32-33	-0.024410962975878192
1104	34-35	-0.0541520157152533
1104	36-37	-0.05567322927780083
1104	38-39	-0.054498609623983896
1104	40-41	-0.018874178058887026
1104	42-43	-0.03679829760724829
1104	44-45	-0.0386174730709854
1104	46-47	-0.06338084571428482
1104	48-49	-0.07198857526686453
1104	50-51	-0.021081675382333742
1104	52-53	-0.030676592132408587
1104	54-55	-0.09702135362274333
1104	56-57	-0.09359714641371397
1104	58-59	-0.11207986617079513
1104	60-61	-0.11958171391426475
1104	62-63	-0.141456211493999
1104	64-65	-0.3679281931922169
1104	66-67	-0.4477345296271551
1104	68-69	-0.3139439556272521
1104	70-71	-0.17590442225295888
1104	72-73	-0.190658033782114
1104	74-75	-0.25592365689842467
1104	76-77	-0.28312927934819143
1104	78-79	-0.13923699644127296
1104	80-81	-0.10765963895806507
1104	82-83	-0.20257443490402594
1104	84-85	-0.18975904646480046
1104	86-87	-0.25424984626707925
1104	88-89	-0.27163410198947346
1104	90-91	-0.2040920811803062
1104	92-93	-0.2164838403227911
1104	94-95	-0.11842496521488854
1104	96-97	-0.11412217832257099
1104	98-99	-0.13283659169698225
1104	100	-0.11572335860279637
1105	1	-0.10654415457468147
1105	2	-0.0671359092767112
1105	3	-0.0856842629425536
1105	4	-0.09429035525956664
1105	5	-0.08994408551911448
1105	6	-0.14212823519861928
1105	7	-0.11561746080840862
1105	8	-0.13537808038322652
1105	9	-0.13454325583602866
1105	10-11	-0.10885619509708988
1105	12-13	-0.11511094160858448
1105	14-15	-0.08026648220080546
1105	16-17	-0.096849460787098
1105	18-19	-0.17164244720886046
1105	20-21	-0.12570488329214413
1105	22-23	-0.11948809650846215
1105	24-25	-0.12700456535745275
1105	26-27	-0.12249754084093212
1105	28-29	-0.12494132805780822
1105	30-31	-0.11741312700037554
1105	32-33	-0.12176105884166333
1105	34-35	-0.15183073478848286
1105	36-37	-0.21473033925499863
1105	38-39	-0.1732699661281174
1105	40-41	-0.11811961982557051
1105	42-43	-0.13805506394406564
1105	44-45	-0.17196722894387761
1105	46-47	-0.13438388260227185
1105	48-49	-0.20192298923727492
1105	50-51	-0.19496402018653214
1105	52-53	-0.16544857974849947
1105	54-55	-0.24856625807469612
1105	56-57	-0.22775973341063604
1105	58-59	-0.19924613670261948
1105	60-61	-0.15943744466962073
1105	62-63	-0.19683285580067889
1105	64-65	-0.27608424787603525
1105	66-67	-0.2077778934925263
1105	68-69	-0.16698318993663364
1105	70-71	-0.12131881237355557
1105	72-73	-0.09547615679355204
1105	74-75	-0.16733037474607926
1105	76-77	-0.20884635123115558
1105	78-79	-0.1455287228153992
1105	80-81	-0.14508773000507347
1105	82-83	-0.18714833887369053
1105	84-85	-0.14091435381381956
1105	86-87	-0.21475565115659379
1105	88-89	-0.2515139554539303
1105	90-91	-0.2241380523460279
1105	92-93	-0.2810422507005441
1105	94-95	-0.1638001051992255
1105	96-97	-0.1947847436008061
1105	98-99	-0.3573638550751248
1105	100	-0.2736533555410965
1106	1	-0.2246588913142311
1106	2	-0.1792739666253098
1106	3	-0.19518905942361542
1106	4	-0.1482826611008221
1106	5	-0.19360798480210661
1106	6	-0.30849041610802175
1106	7	-0.2724252057182994
1106	8	-0.26276652361899266
1106	9	-0.30009307242268335
1106	10-11	-0.30637898241513284
1106	12-13	-0.2899913513042023
1106	14-15	-0.35563096879406686
1106	16-17	-0.3267728503940788
1106	18-19	-0.3421395914510299
1106	20-21	-0.32253604494383126
1106	22-23	-0.2878218240175201
1106	24-25	-0.302804247525998
1106	26-27	-0.25920789173040504
1106	28-29	-0.27387534109757894
1106	30-31	-0.2566825434087221
1106	32-33	-0.2910332110985152
1106	34-35	-0.27473898886562864
1106	36-37	-0.30085765238520423
1106	38-39	-0.3210864494793597
1106	40-41	-0.2799163076328739
1106	42-43	-0.37071801960050266
1106	44-45	-0.3412662543902698
1106	46-47	-0.3104040070455767
1106	48-49	-0.3563782808141127
1106	50-51	-0.37271149283985494
1106	52-53	-0.3294493756749546
1106	54-55	-0.3425688004809686
1106	56-57	-0.35046839059774015
1106	58-59	-0.33930481123100265
1106	60-61	-0.3258377993117705
1106	62-63	-0.28811996737040246
1106	64-65	-0.33063157682011735
1106	66-67	-0.3527701991167973
1106	68-69	-0.40857662690796204
1106	70-71	-0.302388099969523
1106	72-73	-0.39284106782639583
1106	74-75	-0.5288801115823567
1106	76-77	-0.5603086358460949
1106	78-79	-0.4060317516105094
1106	80-81	-0.41320721746686573
1106	82-83	-0.4095161036781114
1106	84-85	-0.4028894265468139
1106	86-87	-0.3250076842946257
1106	88-89	-0.40672937302101175
1106	90-91	-0.31619831610679583
1106	92-93	-0.2696547521762511
1106	94-95	-0.20556000355538373
1106	96-97	-0.22457776228991833
1106	98-99	-0.258936640613058
1106	100	-0.3033291498981612
1107	1	-0.1948337670491469
1107	2	-0.1737858044259113
1107	3	-0.18168931059739535
1107	4	-0.16794076036600103
1107	5	-0.17675780793842222
1107	6	-0.1613698310730456
1107	7	-0.17674913768303924
1107	8	-0.21093675505463239
1107	9	-0.20787446171074464
1107	10-11	-0.21024043539478043
1107	12-13	-0.2179200014452718
1107	14-15	-0.271362622666679
1107	16-17	-0.27220041671674267
1107	18-19	-0.2396934525719132
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2207	58-59	-0.021529042688861466
2207	60-61	0.010522318936438069
2207	62-63	-0.029180133056158297
2207	64-65	0.05104088737697765
2207	66-67	0.11328626526596253
2207	68-69	0.11160875510836021
2207	70-71	0.07691194042414295
2207	72-73	0.008474340475153497
2207	74-75	0.1653903284422391
2207	76-77	0.1592294624646442
2207	78-79	0.02092273611339479
2207	80-81	-0.019264728649854845
2207	82-83	-0.07439895607938851
2207	84-85	0.00870238864935402
2207	86-87	-0.02671606390330794
2207	88-89	0.11336549555990771
2207	90-91	0.06621952991313051
2207	92-93	0.12217546525057088
2207	94-95	0.08717862694946277
2207	96-97	0.12615906740329308
2207	98-99	0.22374872781550792
2207	100	0.1644210003008233
2208	1	-0.4747117205235867
2208	2	-0.9427728896984782
2208	3	-0.5831447411963069
2208	4	-0.5747399842717726
2208	5	-0.6109468045144126
2208	6	-0.650231519453726
2208	7	-0.552436534461954
2208	8	-0.6033562892799722
2208	9	-0.5981199589239523
2208	10-11	-0.591196958139264
2208	12-13	-0.7085000118099316
2208	14-15	-0.6984655086568949
2208	16-17	-1.1257496963881977
2208	18-19	-0.874556010490835
2208	20-21	-0.7689457206401755
2208	22-23	-0.7608195006453542
2208	24-25	-0.7598906757077586
2208	26-27	-0.7539374799390473
2208	28-29	-0.7413524818175858
2208	30-31	-0.7425040108683305
2208	32-33	-0.6823825343915715
2208	34-35	-0.6977075612858314
2208	36-37	-0.7213586630983215
2208	38-39	-0.6443779805802379
2208	40-41	-0.6986036650749625
2208	42-43	-0.6993786019093733
2208	44-45	-0.6799970353628524
2208	46-47	-0.5530196603419384
2208	48-49	-0.5238036199971035
2208	50-51	-0.5852980768694351
2208	52-53	-0.5948504414586466
2208	54-55	-0.49970967368920327
2208	56-57	-0.561688816006022
2208	58-59	-0.6587209888890087
2208	60-61	-1.033517965450656
2208	62-63	-0.6825589040181228
2208	64-65	-1.0370451626252404
2208	66-67	-1.0713400000660442
2208	68-69	-0.6252094167512432
2208	70-71	-1.3088120842376831
2208	72-73	-0.7687926718584919
2208	74-75	-0.3679709686524433
2208	76-77	-0.22437392416489033
2208	78-79	-0.32792593107253865
2208	80-81	-0.2635762372122734
2208	82-83	-0.21044058295759172
2208	84-85	-0.18029771395794825
2208	86-87	-0.11569011069078527
2208	88-89	0.021455544294791906
2208	90-91	-0.1530911875790899
2208	92-93	-0.8203983318750012
2208	94-95	-0.6363650015380884
2208	96-97	-0.35409976590357317
2208	98-99	0.05166876690602962
2208	100	-0.06977891340598319
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	825829.0
3	268314.0
4	165664.0
5	137260.0
6	134930.0
7	150947.0
8	172634.0
9	189241.0
10	206387.0
11	212973.0
12	230736.0
13	281918.0
14	398049.0
15	574565.0
16	657480.0
17	635338.0
18	614312.0
19	503279.0
20	442985.0
21	451106.0
22	477376.0
23	520308.0
24	578145.0
25	647250.0
26	735059.0
27	829364.0
28	943663.0
29	1075982.0
30	1241792.0
31	1435759.0
32	1694950.0
33	2061092.0
34	2613227.0
35	3495937.0
36	5043238.0
37	7559970.0
38	8313851.0
39	2202480.0
40	3798.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.8466131381441	28.098556184680568	27.61739798147495	22.437432695700384
2	23.234336377419943	27.072986856302734	28.429632256850223	21.263044509427104
3	23.31603954831003	27.1545390752369	27.129630984491005	22.399790391962064
4	23.862390399271366	26.215729410960005	27.53372998065675	22.388150209111878
5	24.492867908166442	26.303240418664288	26.203401336787184	23.00049033638209
6	24.62913140091677	26.147584572332555	26.156472924531794	23.06681110221888
7	24.010810828338244	26.724296397574708	25.8584487384032	23.406444035683844
8	24.168543016423392	26.06770825583974	26.302505513097472	23.461243214639396
9	24.03351142247729	26.327057321182785	25.95489539373194	23.684535862607987
10-11	24.425507712163178	26.05850671372697	25.950875781169536	23.565109792940312
12-13	23.872245787351847	26.15572323171349	26.168623642899213	23.803407338035452
14-15	23.443396854189174	26.294816175210194	26.687738026459783	23.57404894414085
16-17	23.239362666682208	26.34378764071517	27.025690766766687	23.391158925835935
18-19	22.803294078287127	26.356462996937935	27.879249089999735	22.960993834775202
20-21	22.24761933063475	26.396199946622907	28.96942980841925	22.386750914323095
22-23	22.037276805840495	26.309876845865443	29.50919446596766	22.143651882326406
24-25	21.771353205894563	26.012644179050643	30.208762124622023	22.007240490432775
26-27	21.74111246118312	26.08325885978215	30.74051064942281	21.43511802961192
28-29	21.749250899981032	26.18620859694243	30.702763622193448	21.36177688088309
30-31	21.70148913181135	26.232383089687	30.635894177773743	21.430233600727906
32-33	21.70638174518682	26.346709079948184	30.538940779600583	21.40796839526442
34-35	21.712006367427758	26.46211478069213	30.500522603189516	21.325356248690593
36-37	21.712397266879858	26.548472026190183	30.357548306501265	21.38158240042869
38-39	21.738654395915248	26.716686522781714	30.14278322279739	21.401875858505647
40-41	21.788666330010567	26.980710327949915	29.77400881930618	21.456614522733336
42-43	21.91283121912148	27.266941571005137	29.20908757812154	21.611139631751843
44-45	22.054174511472596	27.639666414487696	28.627857990410334	21.678301083629375
46-47	22.17723166487821	27.998248406004418	28.037217411473957	21.787302517643415
48-49	22.35590547942726	28.279449965195646	27.40679219930969	21.957852356067402
50-51	22.391652912525693	28.609400603349194	26.91133134314342	22.087615140981693
52-53	22.442582888910053	28.815943428719976	26.660951517007135	22.080522165362833
54-55	22.486231556134307	28.910560270740916	26.49266760457296	22.11054056855182
56-57	22.453328266203457	29.10921311986407	26.352491683840938	22.084966930091536
58-59	22.464727507646238	29.295683137793382	26.21568942873292	22.02389992582746
60-61	22.439777495166993	29.448159824448027	26.0585090530866	22.05355362729838
62-63	22.446632243049606	29.621933914089603	25.966435849593196	21.964997993267595
64-65	22.443999785510076	29.83976730409001	25.80576295425489	21.910469956145022
66-67	22.450573717165952	30.005962923361402	25.6236688345671	21.919794524905544
68-69	22.44159251469051	30.20329426997676	25.45320780119516	21.90190541413757
70-71	22.4240158774988	30.411388901575965	25.29343974907029	21.871155471854948
72-73	22.415408530974382	30.56547876282234	25.124434794136135	21.89467791206714
74-75	22.435920715231898	30.746506244653776	24.989082952516377	21.82849008759795
76-77	22.204288521236048	31.12056895102482	24.725001573049653	21.95014095468948
78-79	22.223036512089525	31.27689136134747	24.543642234844377	21.956429891718624
80-81	22.197301311504543	31.475747807493505	24.390525588649542	21.936425292352414
82-83	22.168866351690248	31.697928841095234	24.227709390046712	21.905495417167806
84-85	22.126981668519864	31.870242110304886	23.985722255917306	22.017053965257947
86-87	22.143477795855514	32.04360473906967	23.805153192008056	22.007764273066755
88-89	22.1575840104546	32.215073536485534	23.56203763112183	22.065304821938035
90-91	22.181904842907365	32.312997493817875	23.358938980331065	22.1461586829437
92-93	22.135108469117284	32.53060295832702	23.093237628790014	22.24105094376568
94-95	22.187933850274653	32.65411129441607	22.861250797551712	22.296704057757562
96-97	22.180480873515805	32.78015785359039	22.62135886556307	22.418002407330732
98-99	22.18995546319643	32.90304210224978	22.43443103856765	22.47257139598614
100	22.632747357589842	32.68484093978917	21.72294311179838	22.959468590822617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13204.0
1	8855.5
2	4161.0
3	3804.5
4	4066.0
5	4636.5
6	5307.5
7	6166.5
8	7246.5
9	8695.0
10	10542.0
11	12916.0
12	16568.5
13	22843.0
14	33661.0
15	46171.5
16	77246.5
17	89965.5
18	83638.5
19	98888.0
20	126313.0
21	154068.5
22	179806.0
23	217185.5
24	260226.0
25	309650.0
26	367868.0
27	430589.0
28	504642.5
29	584314.5
30	651475.5
31	719787.0
32	801942.5
33	891173.0
34	984331.0
35	1101911.0
36	1235385.5
37	1348182.5
38	1453123.5
39	1540988.0
40	1596233.5
41	1647822.0
42	1719292.5
43	1810061.0
44	1904780.0
45	1945611.5
46	1922601.5
47	1869148.5
48	1794623.5
49	1726139.5
50	1652916.5
51	1576625.5
52	1480944.5
53	1360214.0
54	1246624.0
55	1143081.0
56	1034225.5
57	925346.0
58	839009.5
59	758076.5
60	667340.5
61	587251.5
62	512127.0
63	432724.0
64	364156.5
65	306326.0
66	257863.0
67	213951.5
68	174313.5
69	142432.5
70	118599.5
71	102608.5
72	91002.0
73	81299.5
74	71087.0
75	56572.0
76	43483.0
77	36723.5
78	32662.5
79	26072.0
80	16977.5
81	9689.0
82	5542.5
83	3077.0
84	1764.0
85	1126.0
86	754.5
87	485.5
88	304.0
89	199.0
90	118.0
91	72.5
92	49.0
93	39.0
94	28.0
95	18.0
96	12.5
97	6.5
98	2.5
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01103080276251525
2	0.01333547094899053
3	1.0055987634665065E-4
4	0.027811988658159384
5	0.04065286919491434
6	0.0010179122177130353
7	9.994420363432423E-4
8	8.824642210012201E-5
9	5.746278648380038E-4
10-11	0.001004572642279296
12-13	1.2108230009086508E-4
14-15	1.405786026478688E-4
16-17	6.567175598148614E-5
18-19	8.547589489465306E-4
20-21	6.023331368926933E-4
22-23	1.6110102639208318E-4
24-25	2.093287221909871E-4
26-27	3.2322817397137713E-4
28-29	1.0261211872107212E-4
30-31	1.457092085839224E-4
32-33	6.423518631939114E-4
34-35	9.183784625535953E-4
36-37	7.952439200883088E-4
38-39	0.0016520551114092609
40-41	0.0018593315912258266
42-43	0.002603269451953599
44-45	0.0024852655154243663
46-47	0.0026361053299443424
48-49	0.0010794794889456786
50-51	7.131542251114512E-4
52-53	0.0011389945178039004
54-55	0.0033615730093023224
56-57	0.01093126900735581
58-59	0.012981459139402832
60-61	0.014894149032363618
62-63	0.015265604902133897
64-65	0.014264110623416234
66-67	0.002232839703370529
68-69	1.3236963315018302E-4
70-71	0.0017926337140571297
72-73	7.439378607277728E-4
74-75	0.0036704354866527495
76-77	3.735081121447025E-4
78-79	6.998146496777117E-4
80-81	0.0012180058492191259
82-83	0.0029223931411761334
84-85	3.3348938584348435E-4
86-87	5.797584707740573E-4
88-89	0.004567265404274919
90-91	0.009177627898412689
92-93	0.0131507691352926
94-95	0.019176152746593955
96-97	0.025112263814607978
98-99	0.022726532054343048
100	0.015118869572362765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.8727188E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.81868537395351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00423142363535	31.023928095705216
2	12.103091037213613	9.88064530200678
3	4.181826753100155	5.120900115695302
4	2.078796058795747	3.394148891223926
5	1.248023076857504	2.547133065683992
6	0.8021251320040365	1.964501603628821
7	0.560494376420143	1.6015050523474856
8	0.4310843861603797	1.4077038342643533
9	0.32682445579406266	1.2006490170214277
>10	1.86681337186325	14.222810356609505
>50	0.1982955941270072	5.7077413881348775
>100	0.18169565147306393	13.95057311327704
>500	0.011606968454242713	3.2539772657046204
>1k	0.004925364632069177	3.0152824066274317
>5k	3.5286260464578536E-5	0.10752333995822198
>10k+	1.3106320923152154E-4	1.6009771521107146
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	136823	0.28079395839546495	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	123417	0.25328159712397114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79909	0.163992635897643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.104484748842885E-6	0.0	4.104484748842885E-6	0.0
2	0.0	6.156727123264326E-6	0.0	4.104484748842885E-6	0.0
3	0.0	1.4365696620950095E-5	0.0	8.20896949768577E-6	6.156727123264326E-6
4	2.0522423744214423E-6	1.641793899537154E-5	0.0	1.026121187210721E-5	8.20896949768577E-6
5	2.0522423744214423E-6	1.641793899537154E-5	2.0522423744214423E-6	1.641793899537154E-5	1.026121187210721E-5
6	2.0522423744214423E-6	2.052242374421442E-5	2.0522423744214423E-6	2.6679150867478747E-5	1.026121187210721E-5
7	2.0522423744214423E-6	2.2574666118635864E-5	2.0522423744214423E-6	3.0783635616321634E-5	1.4365696620950095E-5
8	4.104484748842885E-6	2.6679150867478747E-5	2.0522423744214423E-6	3.0783635616321634E-5	1.641793899537154E-5
9	4.104484748842885E-6	3.0783635616321634E-5	2.0522423744214423E-6	4.104484748842884E-5	1.641793899537154E-5
10-11	1.94963025570037E-5	3.591424155237524E-5	2.0522423744214423E-6	4.3097089862850286E-5	1.7444060182582257E-5
12-13	9.029866447454344E-5	4.104484748842884E-5	2.0522423744214423E-6	5.438442292216822E-5	2.052242374421442E-5
14-15	2.483213273049945E-4	4.104484748842884E-5	2.0522423744214423E-6	6.567175598148615E-5	3.0783635616321634E-5
16-17	6.125943487648005E-4	4.3097089862850286E-5	2.0522423744214423E-6	7.285460429196119E-5	3.3861999177953795E-5
18-19	0.001199535667849333	4.925381698611461E-5	4.104484748842885E-6	8.208969497685767E-5	4.207096867563956E-5
20-21	0.002037876677800492	5.233218054774677E-5	4.104484748842885E-6	9.33770280361756E-5	4.617545342448245E-5
22-23	0.0035278046416304587	5.6436665296589656E-5	4.104484748842885E-6	1.0363823990828283E-4	4.925381698611461E-5
24-25	0.005514375260070415	5.746278648380038E-5	4.104484748842885E-6	1.118472094059686E-4	5.541054410937894E-5
26-27	0.007747214963440944	5.9515028858221824E-5	4.104484748842885E-6	1.1595169415481147E-4	5.9515028858221824E-5
28-29	0.012000487284429383	6.259339241985399E-5	4.104484748842885E-6	1.2108230009086508E-4	6.464563479427543E-5
30-31	0.015136313632545346	6.36195136070647E-5	4.104484748842885E-6	1.231345424652865E-4	6.567175598148615E-5
32-33	0.01619219233418518	6.464563479427543E-5	4.104484748842885E-6	1.2826514840134012E-4	6.977624073032903E-5
34-35	0.017328108488427446	6.567175598148615E-5	4.104484748842885E-6	1.3955248146065805E-4	7.695908904080409E-5
36-37	0.018577924094450103	6.772399835590759E-5	4.104484748842885E-6	1.4776145095834382E-4	8.516805853848985E-5
38-39	0.019915986122572886	6.772399835590759E-5	4.104484748842885E-6	1.5494429926881889E-4	9.33770280361756E-5
40-41	0.02144285444914244	7.182848310475048E-5	4.104484748842885E-6	1.5802266283045105E-4	1.0055987634665067E-4
42-43	0.02328474198018568	7.79852102280148E-5	4.104484748842885E-6	1.6315326876650463E-4	1.1287333059317931E-4
44-45	0.02564584683195755	7.79852102280148E-5	6.156727123264326E-6	1.7444060182582257E-4	1.169778153420222E-4
46-47	0.028821691906374732	7.901133141522551E-5	6.156727123264326E-6	1.7957120776187618E-4	1.2416066365249725E-4
48-49	0.03280406823393954	8.619417972570057E-5	8.20896949768577E-6	1.8264957132350837E-4	1.313435119629723E-4
50-51	0.03792851744286988	8.927254328733273E-5	8.20896949768577E-6	1.8880629844677266E-4	1.313435119629723E-4
52-53	0.0445151893435755	9.440314922338633E-5	8.20896949768577E-6	1.9598914675724772E-4	1.3339575433739373E-4
54-55	0.05290373004902314	1.026121187210721E-4	8.20896949768577E-6	2.0317199506772276E-4	1.3544799671181518E-4
56-57	0.06381139826907312	1.0363823990828283E-4	8.20896949768577E-6	2.052242374421442E-4	1.3852636027344734E-4
58-59	0.07777998599057265	1.0466436109549355E-4	8.20896949768577E-6	2.0830260100377637E-4	1.467353297711331E-4
60-61	0.09506807575269888	1.077427246571257E-4	8.20896949768577E-6	2.185638128758836E-4	1.5289205689439744E-4
62-63	0.11735953242366459	1.1082108821875788E-4	9.23509068489649E-6	2.195899340630943E-4	1.559704204560296E-4
64-65	0.14498989763168768	1.1595169415481147E-4	1.026121187210721E-5	2.3600787305846585E-4	1.559704204560296E-4
66-67	0.17892475141393344	1.262129060269187E-4	1.026121187210721E-5	2.472952061177838E-4	1.569965416432403E-4
68-69	0.22045495422391292	1.303173907757616E-4	1.026121187210721E-5	2.534519332410481E-4	1.6212714757929392E-4
70-71	0.27189543545997363	1.3442187552460444E-4	1.026121187210721E-5	2.606347815515232E-4	1.6212714757929392E-4
72-73	0.3335817367503333	1.3647411789902592E-4	1.026121187210721E-5	2.6473926630036604E-4	1.6315326876650463E-4
74-75	0.40648149037453174	1.3955248146065805E-4	1.026121187210721E-5	2.7089599342363036E-4	1.6417938995371537E-4
76-77	0.493735653286621	1.4365696620950095E-4	1.026121187210721E-5	2.739743569852625E-4	1.6930999588976895E-4
78-79	0.5962266897076023	1.4468308739671166E-4	1.026121187210721E-5	2.811572052957376E-4	1.7341448063861185E-4
80-81	0.714867847494093	1.5083981451997599E-4	1.026121187210721E-5	2.83209447670159E-4	1.8778017725956195E-4
82-83	0.850587766320519	1.5289205689439744E-4	1.128733305931793E-5	2.883400536062126E-4	1.908585408211941E-4
84-85	1.0050559453584722	1.559704204560296E-4	1.2313454246528652E-5	2.9449678072947694E-4	2.000936315060906E-4
86-87	1.1810367550862981	1.600749052048725E-4	1.2313454246528652E-5	3.0167962903995197E-4	2.0932872219098708E-4
88	1.3258019321779866	1.6212714757929392E-4	1.2313454246528652E-5	3.078363561632163E-4	2.1548544931425143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	41460	0.0	68.07105	2
ATATCTC	45745	0.0	62.277523	1
ATCTCGA	57830	0.0	48.528202	3
TATGGAT	53355	0.0	44.655148	2
ATATGGA	55320	0.0	43.51597	1
TCTCGAG	65150	0.0	42.729538	4
CTCGAGG	75635	0.0	36.61111	5
ATGGATC	75050	0.0	31.755388	3
GGATCCG	76760	0.0	30.329557	5
TGGATCC	81385	0.0	29.173908	4
TCGAGGG	99170	0.0	27.883478	6
GATCCGG	98480	0.0	23.698088	6
CGAGGGC	118820	0.0	23.268164	7
ATCCGGC	104980	0.0	21.921806	7
AGGGCGC	126285	0.0	21.721544	9
GAGGGCG	127055	0.0	21.715664	8
TCCGGCG	115795	0.0	19.748545	8
CCGGCGC	163565	0.0	13.969389	9
GCGCCGT	118850	0.0	13.00126	9
CCGTCGA	117290	0.0	11.968126	16-17
>>END_MODULE