FastQCFastQC Report
Wed 25 May 2016
SRR445727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52518698
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1274810.2427345019101578No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1198540.22821205506655934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG467050.062.7816282
ATATCTC516150.057.5348051
ATCTCGA619600.046.9943963
TATGGAT570600.045.1511652
ATATGGA592100.043.7702061
TCTCGAG710750.040.5972564
CTCGAGG832600.034.5730675
ATGGATC871850.029.6394563
GGATCCG881400.028.627185
TGGATCC989950.026.0227994
TCGAGGG1173900.024.58026
GATCCGG1127650.022.4668986
CGAGGGC1297600.022.1286817
ATCCGGC1212300.020.6930077
GAGGGCG1434700.019.9518838
AGGGCGC1431500.019.8555439
TCCGGCG1350300.018.4877578
GCGCCGT1411550.012.9579989
CCGGCGC1999150.012.5133179
CGTCGAC1385050.012.27989412-13
Produced by FastQC (version 0.11.5)