FastQCFastQC Report
Wed 25 May 2016
SRR445726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49180773
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1275750.2594001521692227No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1242220.25258244720960366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA632250.12855633643659892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG420950.065.811972
ATATCTC454650.061.6966441
ATCTCGA593300.046.3104633
TATGGAT539700.044.275822
ATATGGA576650.041.9089051
TCTCGAG683800.039.8579524
CTCGAGG782900.034.6944925
ATGGATC731400.032.8295443
GGATCCG722050.032.475845
TGGATCC824250.028.9999894
TCGAGGG1060150.025.5636356
GATCCGG948850.024.7486656
CGAGGGC1220350.022.1541337
ATCCGGC1050500.022.0678427
AGGGCGC1317050.020.4636469
GAGGGCG1361300.019.8500448
TCCGGCG1174400.019.6677638
GCGCCGT1239000.013.2602599
CCGGCGC1770000.012.9674419
CGTCGAC1217850.012.2117912-13
Produced by FastQC (version 0.11.5)