##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445726_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49180773
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.500664151822097	33.0	31.0	34.0	30.0	34.0
2	31.780573030033505	34.0	31.0	34.0	30.0	34.0
3	31.877172223380875	34.0	31.0	34.0	30.0	34.0
4	35.25415277632989	37.0	35.0	37.0	33.0	37.0
5	35.03068390161334	37.0	35.0	37.0	32.0	37.0
6	34.97257944685009	37.0	35.0	37.0	32.0	37.0
7	34.92133779597161	37.0	35.0	37.0	32.0	37.0
8	34.87078993248032	37.0	35.0	37.0	32.0	37.0
9	36.49087644474397	39.0	37.0	39.0	32.0	39.0
10-11	36.43898654256614	39.0	37.0	39.0	32.0	39.0
12-13	36.370553152956745	39.0	37.0	39.0	32.0	39.0
14-15	37.617475085233	40.0	38.0	41.0	32.0	41.0
16-17	37.543806184176894	40.0	38.0	41.0	32.0	41.0
18-19	37.48128941568283	40.0	38.0	41.0	32.0	41.0
20-21	37.396301304576895	40.0	38.0	41.0	32.0	41.0
22-23	37.28164388550786	40.0	38.0	41.0	31.5	41.0
24-25	37.145513949119916	40.0	37.5	41.0	31.0	41.0
26-27	36.8987297129307	40.0	37.0	41.0	31.0	41.0
28-29	36.62677810086474	40.0	36.0	41.0	30.0	41.0
30-31	36.36907984345834	40.0	36.0	41.0	30.0	41.0
32-33	36.16171680546786	40.0	35.0	41.0	30.0	41.0
34-35	36.06065457734876	40.0	35.0	41.0	29.5	41.0
36-37	35.85036272203367	39.0	35.0	41.0	28.0	41.0
38-39	35.65808490850682	39.0	35.0	41.0	27.0	41.0
40-41	35.52791292036016	40.0	35.0	41.0	26.0	41.0
42-43	35.26112607054794	40.0	35.0	41.0	24.5	41.0
44-45	34.919288773683974	39.5	35.0	41.0	22.5	41.0
46-47	34.51672984074488	39.0	35.0	41.0	19.0	41.0
48-49	34.10618326393528	39.0	34.0	41.0	11.5	41.0
50-51	33.77547972863297	39.0	34.0	41.0	7.5	41.0
52-53	33.442293292136746	39.0	33.0	41.0	2.0	41.0
54-55	33.134411510774754	38.0	33.0	41.0	2.0	41.0
56-57	32.73224445862208	38.0	33.0	40.0	2.0	41.0
58-59	32.35520979916277	37.5	32.0	40.0	2.0	41.0
60-61	31.9598399663218	37.0	31.5	40.0	2.0	41.0
62-63	31.54951394318263	36.0	31.0	40.0	2.0	41.0
64-65	31.040435395352567	35.5	30.5	39.5	2.0	41.0
66-67	30.612625313961615	35.0	30.0	39.0	2.0	41.0
68-69	30.124630513635886	35.0	29.5	39.0	2.0	41.0
70-71	29.610623444247206	35.0	29.0	38.0	2.0	40.0
72-73	29.06955654194374	35.0	28.0	37.0	2.0	40.0
74-75	28.534332166759558	34.5	27.0	36.5	2.0	39.0
76-77	27.7936795747395	34.0	26.0	36.0	2.0	39.0
78-79	27.737414049998765	34.0	26.0	36.0	2.0	38.5
80-81	27.33017883838467	34.0	25.5	35.0	2.0	37.0
82-83	26.852949789951452	34.0	24.5	35.0	2.0	37.0
84-85	26.366298227968073	34.0	22.5	35.0	2.0	36.5
86-87	25.878196251612394	33.5	19.5	35.0	2.0	36.0
88-89	25.365640887750992	33.0	15.5	35.0	2.0	36.0
90-91	24.846891782282476	33.0	4.5	35.0	2.0	35.0
92-93	24.318067204840396	33.0	2.0	35.0	2.0	35.0
94-95	23.69665113844388	32.5	2.0	35.0	2.0	35.0
96-97	23.05431028910424	32.0	2.0	35.0	2.0	35.0
98-99	22.452761936051715	31.5	2.0	35.0	2.0	35.0
100	21.368922952878354	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3007681769045547
1101	2	0.29967162640235756
1101	3	0.2950953938790519
1101	4	0.2805491875028423
1101	5	0.3202398031580742
1101	6	0.34155683515511726
1101	7	0.36632401492470734
1101	8	0.37211729138456207
1101	9	0.454806944697296
1101	10-11	0.4414359386634672
1101	12-13	0.43687513282263524
1101	14-15	0.4729344851771913
1101	16-17	0.4722280583288452
1101	18-19	0.48410558856931374
1101	20-21	0.44998400543421724
1101	22-23	0.46109652494927644
1101	24-25	0.5029976341957578
1101	26-27	0.47936251647951167
1101	28-29	0.4792127883200976
1101	30-31	0.4650607414996841
1101	32-33	0.4751737882436444
1101	34-35	0.446031784889243
1101	36-37	0.4359730512945461
1101	38-39	0.45425982357846806
1101	40-41	0.4654477547773439
1101	42-43	0.4028129378980836
1101	44-45	0.383249131709988
1101	46-47	0.40205660228779294
1101	48-49	0.4280453703059024
1101	50-51	0.3803978431896269
1101	52-53	0.3426704668524181
1101	54-55	0.30166725539659467
1101	56-57	0.2846949322235801
1101	58-59	0.30557272798992585
1101	60-61	0.22451370006226767
1101	62-63	0.28293724726539793
1101	64-65	0.3034975912287088
1101	66-67	0.22322760662143537
1101	68-69	0.23350553561638066
1101	70-71	0.16163855308954211
1101	72-73	0.18256613003243416
1101	74-75	0.09996148506953517
1101	76-77	0.21692660539750364
1101	78-79	0.2753469912407063
1101	80-81	0.2885667819409896
1101	82-83	0.22947217779382
1101	84-85	0.19613555029591012
1101	86-87	0.24570590529463132
1101	88-89	0.1879571035864167
1101	90-91	0.18435490651950914
1101	92-93	0.039405026662940656
1101	94-95	-0.013014633580048951
1101	96-97	-0.014185510261174983
1101	98-99	0.12167524910954697
1101	100	0.14912510188679562
1102	1	0.09109431305710913
1102	2	0.08793440324421908
1102	3	0.07916496769394854
1102	4	0.07976686837287161
1102	5	0.1595993658738024
1102	6	0.1433644635134712
1102	7	0.15426961696056907
1102	8	0.1532705487600694
1102	9	0.18855581220041273
1102	10-11	0.19178686266671008
1102	12-13	0.16770695864328644
1102	14-15	0.1862112723861813
1102	16-17	0.15940554344216196
1102	18-19	0.16004510655461957
1102	20-21	0.14934504456013542
1102	22-23	0.18382363340142405
1102	24-25	0.19388265307028973
1102	26-27	0.18240460160926375
1102	28-29	0.21475705403840095
1102	30-31	0.20429676395824004
1102	32-33	0.1922379787459505
1102	34-35	0.15476329717944282
1102	36-37	0.1417609345929165
1102	38-39	0.14253350001274612
1102	40-41	0.14177151115514164
1102	42-43	0.14625518515660474
1102	44-45	0.10717487403663739
1102	46-47	0.13274519474300916
1102	48-49	0.1301813642297489
1102	50-51	0.15520176263468244
1102	52-53	0.10610347778014528
1102	54-55	0.09454876155409409
1102	56-57	0.12238449141879926
1102	58-59	0.11655265932287762
1102	60-61	0.0574598112773117
1102	62-63	0.07590746787976599
1102	64-65	0.10437341468561456
1102	66-67	0.038179080855780256
1102	68-69	0.0047705811356664185
1102	70-71	-0.007574601531764813
1102	72-73	-0.04678066466653874
1102	74-75	-0.11156530346437421
1102	76-77	-0.016402417544522763
1102	78-79	-0.04184726743834588
1102	80-81	0.06928623382642485
1102	82-83	0.03088732577767317
1102	84-85	-0.03072434770396626
1102	86-87	-0.03118309938964714
1102	88-89	-0.021293190101552284
1102	90-91	-0.06375242648766388
1102	92-93	-0.1551543017321606
1102	94-95	-0.19811906652039113
1102	96-97	-0.21906399911923202
1102	98-99	-0.14849959898620568
1102	100	-0.27096461872094935
1103	1	0.049916157707755815
1103	2	0.06284299138359017
1103	3	0.06435222171421984
1103	4	0.04948163254941562
1103	5	0.09119230448532534
1103	6	0.07578937255907192
1103	7	0.10019893162925797
1103	8	0.11759891657523269
1103	9	0.035795974199118064
1103	10-11	0.06558049138050848
1103	12-13	0.0356395903446014
1103	14-15	0.10208949241030041
1103	16-17	0.09342784900263723
1103	18-19	0.142031666396349
1103	20-21	0.12463488041471038
1103	22-23	0.1547449955406961
1103	24-25	0.10837536407692028
1103	26-27	0.11948433907420508
1103	28-29	0.010678907978956431
1103	30-31	0.03546600549796608
1103	32-33	-0.05317628410274011
1103	34-35	-0.06213258615190398
1103	36-37	-0.012003378882191384
1103	38-39	0.049680002590960726
1103	40-41	0.01324121399551359
1103	42-43	0.039056294916377965
1103	44-45	0.014098198591824485
1103	46-47	-0.1129663977239872
1103	48-49	0.02159329689968814
1103	50-51	0.04690686448937953
1103	52-53	-0.04708170179430482
1103	54-55	0.036143781016974685
1103	56-57	0.08344887396295775
1103	58-59	0.11095888911737006
1103	60-61	-0.07963240823487894
1103	62-63	0.007843208877581276
1103	64-65	0.029812186913247274
1103	66-67	0.01780374309888444
1103	68-69	0.08357205976319548
1103	70-71	0.004163304084514152
1103	72-73	0.03807819114580724
1103	74-75	-0.003453000404054052
1103	76-77	0.052841831011164686
1103	78-79	-0.06350183875479942
1103	80-81	0.004259196588879632
1103	82-83	0.02519580386106668
1103	84-85	0.004057351205972282
1103	86-87	0.014695636456558248
1103	88-89	0.06279346079788439
1103	90-91	0.07667724426196187
1103	92-93	-0.09754545284702942
1103	94-95	-0.16683180013808396
1103	96-97	-0.2267032031554841
1103	98-99	-0.050429362461368754
1103	100	-0.1728290553076306
1104	1	0.020437867614550242
1104	2	0.02396108461998736
1104	3	-0.00828590328331913
1104	4	-0.029712780522345383
1104	5	-0.0049376653253787595
1104	6	0.022707835938689414
1104	7	0.032241013433043975
1104	8	0.039262701304387804
1104	9	0.051793175103199474
1104	10-11	0.01742977902519982
1104	12-13	0.02908948384278176
1104	14-15	0.041503037927910214
1104	16-17	0.03275029636058946
1104	18-19	0.03981191477637225
1104	20-21	0.024354759338891085
1104	22-23	-0.027936833229226465
1104	24-25	0.012501628729737035
1104	26-27	0.039894179624269555
1104	28-29	0.03531356137385444
1104	30-31	0.04065666200021667
1104	32-33	0.013510201028175572
1104	34-35	0.03306770939477843
1104	36-37	0.04847843457070411
1104	38-39	0.0023590708324903176
1104	40-41	0.07266200400123068
1104	42-43	0.09287035822013934
1104	44-45	0.05651688771766317
1104	46-47	0.007474270060022548
1104	48-49	0.04924252518238603
1104	50-51	0.015566413491036712
1104	52-53	0.05127849079757851
1104	54-55	0.009286700208058107
1104	56-57	0.029256829593684586
1104	58-59	0.013220664961252737
1104	60-61	-0.013329777328550563
1104	62-63	-0.007285305881335802
1104	64-65	0.01384872466494258
1104	66-67	-0.06021747668626176
1104	68-69	-0.04893057187486605
1104	70-71	-0.057740527532352814
1104	72-73	-0.05400095288151263
1104	74-75	-0.1419568854797042
1104	76-77	-0.10615245843231946
1104	78-79	-0.08141026965327924
1104	80-81	0.010052591955869161
1104	82-83	-0.06972448727815461
1104	84-85	-0.0857192319583362
1104	86-87	-0.053549711594527594
1104	88-89	-0.08564258596401686
1104	90-91	-0.08287995246489643
1104	92-93	-0.2260576902666962
1104	94-95	-0.26861100887263945
1104	96-97	-0.2672801491315866
1104	98-99	-0.18699083783628723
1104	100	-0.22285263800061372
1105	1	-0.060039696762000716
1105	2	-0.053272884159333955
1105	3	-0.0288579390687822
1105	4	-0.05669266236581194
1105	5	-0.08019367480208217
1105	6	-0.05900387637861826
1105	7	-0.058634037437443
1105	8	-0.02924809943563389
1105	9	-0.02886745790312517
1105	10-11	-0.012113305780225403
1105	12-13	-0.01266316061519035
1105	14-15	-0.03306305466296067
1105	16-17	-0.07693624117001008
1105	18-19	-0.04237069552374351
1105	20-21	-0.06129601112597527
1105	22-23	-0.03572844725167812
1105	24-25	-0.028613472547121432
1105	26-27	-0.01782096339135819
1105	28-29	-0.07054579759144985
1105	30-31	-0.03526133329525294
1105	32-33	-0.06322573698972889
1105	34-35	-0.07211695275469765
1105	36-37	-0.09553470186353508
1105	38-39	-0.03866070651648812
1105	40-41	-0.07811013524614907
1105	42-43	-0.04385480136188846
1105	44-45	-0.024189448186113793
1105	46-47	-0.05239970015483664
1105	48-49	-0.07084806355490514
1105	50-51	-0.062234108921174425
1105	52-53	-0.03045903922556903
1105	54-55	-0.007540181846451333
1105	56-57	-0.03842627542525889
1105	58-59	-0.00759735612466983
1105	60-61	-0.02955340840509635
1105	62-63	-0.04499908204674696
1105	64-65	-0.07542209250010856
1105	66-67	-0.1752556296797536
1105	68-69	-0.06300047977224565
1105	70-71	-0.08335617873779455
1105	72-73	-0.06628104808094548
1105	74-75	-0.17322958615979545
1105	76-77	-0.07241177890609052
1105	78-79	-0.20291363193069856
1105	80-81	-0.06341264622770382
1105	82-83	-0.08163738822539912
1105	84-85	-0.04668305701700959
1105	86-87	0.034871678363579406
1105	88-89	0.030633749307916958
1105	90-91	-0.07240255474744117
1105	92-93	-0.13121291606344343
1105	94-95	-0.1096912184166321
1105	96-97	-0.18375067199679762
1105	98-99	-0.09806383431269339
1105	100	-0.12127013289359923
1106	1	-0.15501574213173797
1106	2	-0.14008923262207418
1106	3	-0.12519364316726111
1106	4	-0.13078750283521146
1106	5	-0.13489023580365256
1106	6	-0.15510804661776945
1106	7	-0.21202922697511184
1106	8	-0.16229381986611457
1106	9	-0.2303220079327133
1106	10-11	-0.19623192553366664
1106	12-13	-0.17772790558332474
1106	14-15	-0.17473889298781842
1106	16-17	-0.18805537015212792
1106	18-19	-0.20041245349845838
1106	20-21	-0.22160508841420778
1106	22-23	-0.19293929037805668
1106	24-25	-0.15680151147658705
1106	26-27	-0.165738884855827
1106	28-29	-0.12712197336152542
1106	30-31	-0.15976125321618895
1106	32-33	-0.1500458085430907
1106	34-35	-0.1518308764093632
1106	36-37	-0.15720694734659446
1106	38-39	-0.18838358392792998
1106	40-41	-0.10551462441048187
1106	42-43	-0.10706570265261206
1106	44-45	-0.1499138637686812
1106	46-47	-0.1618884682637045
1106	48-49	-0.10294698321599327
1106	50-51	-0.15678292730987664
1106	52-53	-0.10140770892049034
1106	54-55	-0.12285439781077656
1106	56-57	-0.10830633227569564
1106	58-59	-0.10016932427164704
1106	60-61	-0.18976478215756032
1106	62-63	-0.1639643773348496
1106	64-65	-0.19626829003667368
1106	66-67	-0.21058648335374386
1106	68-69	-0.19375516783819435
1106	70-71	-0.18526933878023755
1106	72-73	-0.23155125917370256
1106	74-75	-0.29173672751593216
1106	76-77	-0.21330825556492528
1106	78-79	-0.15261658662993938
1106	80-81	-0.09620253263523892
1106	82-83	-0.17898602238352268
1106	84-85	-0.10914949288585163
1106	86-87	-0.08468350843854111
1106	88-89	-0.07290785344877548
1106	90-91	-0.0894138931771522
1106	92-93	-0.2042664988639089
1106	94-95	-0.19173277493678498
1106	96-97	-0.17610555588899857
1106	98-99	-0.04597427403809107
1106	100	-0.04931738061419466
1107	1	-0.224135235458089
1107	2	-0.21262939536497782
1107	3	-0.18739136706106763
1107	4	-0.1788361092057471
1107	5	-0.20354859330819863
1107	6	-0.2461946215503943
1107	7	-0.23756719713715313
1107	8	-0.30991045760970337
1107	9	-0.2277660978070486
1107	10-11	-0.2650811347269979
1107	12-13	-0.23987276839520888
1107	14-15	-0.32269985975355553
1107	16-17	-0.27515418939616154
1107	18-19	-0.26947692998883355
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2207	56-57	-0.010085787121433043
2207	58-59	0.03422458712620369
2207	60-61	0.11789654645734515
2207	62-63	0.07221844745759398
2207	64-65	0.12057763063485183
2207	66-67	0.12950683593393109
2207	68-69	0.07980550126034203
2207	70-71	-0.030086238559711376
2207	72-73	0.0733838950976704
2207	74-75	0.09723804175625617
2207	76-77	0.0227772464595688
2207	78-79	0.042262388574730636
2207	80-81	0.05237435091896003
2207	82-83	0.06965018899612474
2207	84-85	0.012268800613753683
2207	86-87	-0.015613838106613542
2207	88-89	0.06383592543709327
2207	90-91	0.07250589988647604
2207	92-93	0.21953163824516508
2207	94-95	0.20622622557028336
2207	96-97	0.28228292752534045
2207	98-99	0.27933383314626425
2207	100	0.31718321744041234
2208	1	-0.19641183294086773
2208	2	-0.19404122111625455
2208	3	-0.203600675585065
2208	4	-0.17520538587831425
2208	5	-0.17289881081385516
2208	6	-0.2725912597810307
2208	7	-0.21022189589127294
2208	8	-0.23935698247822046
2208	9	-0.22611826007426572
2208	10-11	-0.25732760751004236
2208	12-13	-0.2494861512703892
2208	14-15	-0.2748339860851914
2208	16-17	-0.27841924936617346
2208	18-19	-0.2981172173256823
2208	20-21	-0.27746978854526105
2208	22-23	-0.2632584594165195
2208	24-25	-0.27802158863709536
2208	26-27	-0.367981975986055
2208	28-29	-0.26931010404605615
2208	30-31	-0.2674896113017269
2208	32-33	-0.19477287028963275
2208	34-35	-0.17284369803445543
2208	36-37	-0.14264416844647343
2208	38-39	-0.17251744701273708
2208	40-41	-0.2967389560584337
2208	42-43	-0.20607591223686939
2208	44-45	-0.2053037978751604
2208	46-47	-0.09295279461844785
2208	48-49	-0.06583284176547721
2208	50-51	-0.03767681275128609
2208	52-53	-0.043311965367685445
2208	54-55	-0.042492595402777056
2208	56-57	4.895788842418369E-5
2208	58-59	0.07813701181873256
2208	60-61	0.11646648944124394
2208	62-63	-0.06006264818079643
2208	64-65	0.11304699036822186
2208	66-67	0.09621733413455757
2208	68-69	0.05676224875040603
2208	70-71	0.09589860024674834
2208	72-73	0.11524663252951228
2208	74-75	0.2846395024079271
2208	76-77	0.23761078061280472
2208	78-79	0.25206724299535566
2208	80-81	0.3029104345612339
2208	82-83	0.37839988884719133
2208	84-85	0.3504502748396874
2208	86-87	0.34437973176445524
2208	88-89	0.45369770517047314
2208	90-91	0.5087044103476934
2208	92-93	0.6795882748812403
2208	94-95	0.26035188846481105
2208	96-97	0.49140155677116937
2208	98-99	0.7566646620198405
2208	100	0.7797266680465818
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	744667.0
3	241031.0
4	152117.0
5	126955.0
6	124327.0
7	139416.0
8	157489.0
9	174731.0
10	190863.0
11	197277.0
12	213700.0
13	262673.0
14	381721.0
15	581890.0
16	675629.0
17	655771.0
18	626582.0
19	499753.0
20	428406.0
21	434411.0
22	462373.0
23	501720.0
24	555543.0
25	626549.0
26	705845.0
27	799059.0
28	908970.0
29	1033277.0
30	1198849.0
31	1390920.0
32	1645433.0
33	2011952.0
34	2581025.0
35	3502706.0
36	5155929.0
37	7909273.0
38	8824265.0
39	2353215.0
40	4461.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.932739936473393	27.927828497882878	27.63845128515936	22.500980280484374
2	23.382733356800024	26.903517542773326	28.412618815363324	21.30113028506333
3	23.460568358168267	26.90063298889072	27.12555423077106	22.513244422169954
4	24.004249914672194	26.024584578671835	27.451330605284348	22.51983490137163
5	24.689264599121476	26.15758841279185	26.124808134292493	23.02833885379418
6	24.825959710034322	25.987129129991114	26.04131458255444	23.14559657742013
7	24.22785608575608	26.549176230976613	25.765836166939355	23.457131516327955
8	24.32067763348437	25.896475540571917	26.216837501232014	23.566009324711693
9	24.21582655019562	26.125364654931676	25.87853367382062	23.78027512105209
10-11	24.608406595975254	25.868125678762137	25.846690790625676	23.67677693463693
12-13	24.0502287258792	26.00385919159429	26.074741078274393	23.87117100425211
14-15	23.62446703673257	26.148740537801153	26.588858581257142	23.63793384420914
16-17	23.397055280209123	26.198554776752626	26.94210503401801	23.462284909020244
18-19	22.927614328652563	26.20413169187651	27.862762759266595	23.00549122020433
20-21	22.36032122952599	26.24050324671198	28.973008036838603	22.426167486923422
22-23	22.171753489234472	26.12900543413873	29.532663499840567	22.166577576786224
24-25	21.899853902532563	25.843402762630003	30.2646605831237	21.99208275171374
26-27	21.86884745491618	25.913648338083732	30.773582111497504	21.443922095502575
28-29	21.877688180037016	26.034860005838755	30.719854143953544	21.36759767017068
30-31	21.82932255564358	26.070980154112295	30.65825814592984	21.441439144314288
32-33	21.828463990408938	26.193750518512736	30.556148625676915	21.421636865401414
34-35	21.82842715649997	26.309527107734144	30.513513339026492	21.348532396739394
36-37	21.831199336672082	26.395948546253557	30.372892382479545	21.399959734594816
38-39	21.877788274105313	26.558445885417125	30.142260427706695	21.421505412770863
40-41	21.935376933219892	26.847402823586574	29.74559830540396	21.471621937789575
42-43	22.076528687953562	27.14372611580757	29.13498544590862	21.644759750330252
44-45	22.23837528939208	27.535772487256573	28.49577342911874	21.73007879423261
46-47	22.36496146524378	27.89510573098631	27.8696236689641	21.87030913480581
48-49	22.55203300671762	28.161365461057702	27.242373487952694	22.044228044271986
50-51	22.583692363999116	28.50477963720084	26.734361947544556	22.177166051255483
52-53	22.645092783192602	28.691865988634046	26.462516053392566	22.20052517478079
54-55	22.70128073901852	28.78950404967936	26.296544329059053	22.21267088224307
56-57	22.651779342954168	29.006526797339284	26.142302408649577	22.19939145105697
58-59	22.6613741614823	29.185894578245065	26.010685381936767	22.14204587833587
60-61	22.635387813278772	29.353469639009322	25.85051523615489	22.160627311557015
62-63	22.663856817526742	29.514727078382496	25.7528441597417	22.06857194434906
64-65	22.651414593207488	29.73178891698399	25.598274289274332	22.018522200534186
66-67	22.66058154460607	29.877027113657967	25.425935412887807	22.03645592884816
68-69	22.65625535377349	30.08123470835199	25.253015331651657	22.009494606222862
70-71	22.63533201977547	30.28418897087606	25.10243968081524	21.97803932853323
72-73	22.608940778701413	30.44346110095172	24.93253306975097	22.015065050595904
74-75	22.646145885643982	30.621356149031808	24.80841695309924	21.92408101222497
76-77	22.42777111985939	30.991776297629333	24.542604327836965	22.037848254674316
78-79	22.44669002053875	31.150195281219677	24.359350548924176	22.043764149317397
80-81	22.422761858321046	31.358226685538494	24.175890987452128	22.043120468688336
82-83	22.395254512539417	31.55495659289166	24.017988576631012	22.031800317937908
84-85	22.362335555752292	31.73477876711156	23.792682345097777	22.110203332038374
86-87	22.400830127702335	31.890517937181663	23.604617979302116	22.104033955813886
88-89	22.44667896593806	32.0162267665132	23.362390838137443	22.1747034294113
90-91	22.43803227014685	32.15898905562524	23.157635774609663	22.245342899618247
92-93	22.418358445696178	32.36428385087177	22.87952212812956	22.33783557530249
94-95	22.498952050095923	32.4462711217916	22.66271780389647	22.392059024216007
96-97	22.4760852179026	32.62083945382148	22.379272119033775	22.523803209242146
98-99	22.48366493340895	32.74852494661888	22.203154226962365	22.564655893009807
100	22.988027988753075	32.441196252241724	21.53000606483856	23.04076969416664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7946.0
1	5576.5
2	2963.0
3	2676.5
4	2742.5
5	3045.0
6	3536.5
7	4252.0
8	5052.0
9	6142.0
10	7646.5
11	9614.0
12	12466.5
13	17246.0
14	25825.5
15	35548.0
16	59157.0
17	70138.0
18	68132.0
19	83211.5
20	108516.0
21	133555.0
22	157928.0
23	192762.5
24	233527.0
25	281599.0
26	336704.0
27	396769.5
28	467836.5
29	542600.5
30	610733.0
31	686876.5
32	778645.0
33	876993.0
34	976128.0
35	1092478.5
36	1223403.5
37	1347926.5
38	1467524.0
39	1561044.5
40	1622352.5
41	1686007.0
42	1779072.5
43	1885167.0
44	1986775.0
45	2037105.5
46	2027219.5
47	1975937.0
48	1890503.5
49	1814449.0
50	1737139.5
51	1647930.5
52	1534408.0
53	1391842.0
54	1255862.5
55	1137587.0
56	1013661.5
57	899978.5
58	822674.0
59	749976.5
60	659316.0
61	576058.0
62	501004.5
63	427322.0
64	361973.0
65	304528.0
66	258050.5
67	215215.5
68	175304.5
69	144584.5
70	122113.5
71	106975.0
72	96012.0
73	87281.0
74	77909.5
75	62804.5
76	49208.5
77	42438.5
78	37839.0
79	30224.5
80	20009.5
81	11599.0
82	6375.0
83	3294.0
84	1803.5
85	1131.0
86	796.0
87	540.0
88	330.5
89	204.5
90	130.5
91	84.5
92	59.5
93	37.5
94	23.5
95	19.5
96	11.5
97	7.5
98	8.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01753124132473477
2	0.009522013816252948
3	9.088917736205569E-4
4	0.03544677917120172
5	0.022295298205255946
6	0.006846171368636276
7	0.006411041973659096
8	0.006657073080164885
9	0.007730663363099234
10-11	0.008003127563692421
12-13	0.007916711679175925
14-15	0.008757487402648185
16-17	0.00759239794787284
18-19	0.012943066185641287
20-21	0.008332524582320006
22-23	0.0021115975545972
24-25	0.00189809948696821
26-27	0.002907640349613862
28-29	0.0015768357280598253
30-31	0.0013419878536679365
32-33	0.003361069578959241
34-35	0.0037931490015417207
36-37	0.004229295053983799
38-39	0.005542816498634538
40-41	0.004731523841644375
42-43	0.0057929142349999255
44-45	0.003258387175004346
46-47	0.002194963466718996
48-49	0.005167669894086456
50-51	0.004893172378563468
52-53	0.003103855240339553
54-55	0.005453350641723342
56-57	0.022411197156254538
58-59	0.034127157781761584
60-61	0.027574800420481393
62-63	0.02500367369174942
64-65	0.026142330052437362
66-67	0.01036075622479541
68-69	0.008153592868497614
70-71	0.0017405175799087174
72-73	0.0060511452310845135
74-75	0.006923437335968672
76-77	0.0042130285345454
78-79	0.007094235790071864
80-81	0.004054429970021008
82-83	0.0034027525350201386
84-85	8.682254750245588E-4
86-87	7.889261927623627E-4
88-89	0.0023708452081466877
90-91	0.009625712877672743
92-93	0.02088316098650991
94-95	0.031988110475612085
96-97	0.040808630641084066
98-99	0.02379995125330787
100	0.014566668157086508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.9180773E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.812902477057975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95379997983123	31.407079338158777
2	11.760055059738685	9.599239605558942
3	4.0131069909335055	4.913596327529063
4	2.0166962373997155	3.292289074513773
5	1.1520860543467286	2.350998789061577
6	0.7763988466328722	1.9012254246556621
7	0.5444243197367689	1.5553675667288764
8	0.3990794927750919	1.3030073935378872
9	0.29835856856308157	1.0959191245763695
>10	1.7276935840846317	13.03463963173989
>50	0.16820052729888169	4.744910719644486
>100	0.1700893181069087	15.443827244883229
>500	0.01341334910430209	3.642255145199892
>1k	0.0064230339669065295	4.03933337151091
>5k	4.490685315695708E-5	0.1276570619685901
>10k+	1.2973062753356143E-4	1.5486541807320686
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	127575	0.2594001521692227	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	124222	0.25258244720960366	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63225	0.12855633643659892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0333149297999035E-6	0.0	0.0	2.0333149297999035E-6	0.0
2	2.0333149297999035E-6	0.0	0.0	1.6266519438399228E-5	0.0
3	2.0333149297999035E-6	2.0333149297999035E-6	0.0	1.6266519438399228E-5	2.0333149297999035E-6
4	2.0333149297999035E-6	8.133259719199614E-6	0.0	1.8299834368199134E-5	4.066629859599807E-6
5	2.0333149297999035E-6	1.4233204508599326E-5	0.0	2.2366464227798942E-5	1.2199889578799422E-5
6	2.0333149297999035E-6	2.0333149297999036E-5	0.0	2.4399779157598844E-5	1.2199889578799422E-5
7	2.0333149297999035E-6	2.2366464227798942E-5	0.0	2.8466409017198652E-5	2.0333149297999036E-5
8	2.0333149297999035E-6	2.4399779157598844E-5	0.0	3.2533038876798457E-5	2.2366464227798942E-5
9	8.133259719199614E-6	2.8466409017198652E-5	0.0	4.269961352579798E-5	2.4399779157598844E-5
10-11	3.2533038876798457E-5	3.2533038876798457E-5	0.0	4.778290085029774E-5	2.8466409017198652E-5
12-13	1.2708218311249399E-4	3.863298366619817E-5	0.0	5.7949475499297255E-5	3.2533038876798457E-5
14-15	2.8873072003158634E-4	4.2699613525797975E-5	0.0	7.116602254299663E-5	3.659966873639827E-5
16-17	6.659106395094684E-4	5.08328732449976E-5	0.0	8.23492546568961E-5	4.1682956060898023E-5
18-19	0.0012809884057739395	6.404942028869696E-5	0.0	9.048251437609572E-5	4.981621578009764E-5
20-21	0.002183780234605097	6.811605014829678E-5	0.0	1.0471571888469503E-4	5.591616056949735E-5
22-23	0.0036355670944822277	6.913270761319673E-5	0.0	1.118323211389947E-4	5.998279042909716E-5
24-25	0.005410651028197544	7.421599493769649E-5	0.0	1.1589895099859451E-4	6.709939268339683E-5
26-27	0.007493782173777545	7.828262479729629E-5	0.0	1.1996558085819431E-4	7.319933747279654E-5
28-29	0.011512629132527055	8.438256958669601E-5	0.0	1.2809884057739392E-4	7.624930986749639E-5
30-31	0.014636817522164607	9.048251437609572E-5	0.0	1.3216547043699373E-4	8.336591212179605E-5
32-33	0.01564432506988046	9.658245916549543E-5	0.0	1.4334870255089323E-4	9.251582930589562E-5
34-35	0.01671181540802541	1.0064908902509523E-4	0.0	1.4639867494559307E-4	9.353248677079557E-5
36-37	0.018044653344509245	1.0878234874429485E-4	0.0	1.5351527719989272E-4	9.759911663039538E-5
38-39	0.01944967396100098	1.118323211389947E-4	0.0	1.6164853691909232E-4	1.0268240395489513E-4
40-41	0.021169858391611698	1.1996558085819431E-4	0.0	1.6774848170849206E-4	1.0471571888469503E-4
42-43	0.023403454841996893	1.2708218311249396E-4	0.0	1.7486508396279173E-4	1.05732376349595E-4
44-45	0.02615656325694596	1.331821279018937E-4	0.0	1.779150563574916E-4	1.077656912793949E-4
46-47	0.029656914908596496	1.4334870255089323E-4	0.0	1.8096502875219144E-4	1.1284897860389466E-4
48-49	0.03432235601502237	1.5148196227009284E-4	2.0333149297999035E-6	1.8503165861179124E-4	1.1691560846349446E-4
50-51	0.040191519559889796	1.5453193466479268E-4	2.0333149297999035E-6	1.962148907256907E-4	1.2301555325289415E-4
52-53	0.047626335600703146	1.5656524959459258E-4	2.0333149297999035E-6	1.9824820565549062E-4	1.2606552564759402E-4
54-55	0.05738014731895329	1.6368185184889225E-4	2.0333149297999035E-6	2.0638146537469023E-4	1.2809884057739392E-4
56-57	0.0696674694397341	1.6978179663829196E-4	2.0333149297999035E-6	2.1654804002368977E-4	1.3623210029659356E-4
58-59	0.08545310989723565	1.7689839889259163E-4	2.0333149297999035E-6	2.216313273481895E-4	1.413153876210933E-4
60-61	0.1057710093332612	1.8096502875219144E-4	2.0333149297999035E-6	2.2671461467268925E-4	1.453820174806931E-4
62-63	0.131268778552952	1.860483160766912E-4	2.0333149297999035E-6	2.3281455946208899E-4	1.5148196227009284E-4
64-65	0.1624862626701699	1.9113160340119095E-4	6.099944789399711E-6	2.3586453185678882E-4	1.5554859212969264E-4
66-67	0.20158792542768694	1.9519823326079075E-4	6.099944789399711E-6	2.3789784678658873E-4	1.6368185184889225E-4
68-69	0.25003877023242393	2.0129817805019046E-4	6.099944789399711E-6	2.4298113411108847E-4	1.7588174142769167E-4
70-71	0.30836542565119907	2.0943143776939007E-4	6.099944789399711E-6	2.5009773636538817E-4	1.7893171382239153E-4
72-73	0.3786581394318467	2.155313825587898E-4	6.099944789399711E-6	2.622976259441876E-4	1.819816862170914E-4
74-75	0.4631362748202432	2.2976458706738912E-4	6.099944789399711E-6	2.7348085805808703E-4	1.860483160766912E-4
76-77	0.5625928246390108	2.3383121692698892E-4	6.099944789399711E-6	2.7856414538258685E-4	1.9214826086609089E-4
78-79	0.6786676573790331	2.3789784678658873E-4	6.099944789399711E-6	2.8466409017198653E-4	2.0129817805019046E-4
80-81	0.8121446159457477	2.409478191812886E-4	6.099944789399711E-6	2.877140625666864E-4	2.0841478030449013E-4
82-83	0.965736142455508	2.450144490408884E-4	6.099944789399711E-6	2.907640349613862E-4	2.1146475269919E-4
84-85	1.1406937422476058	2.4806442143558824E-4	6.099944789399711E-6	2.94830664820986E-4	2.1654804002368977E-4
86-87	1.3374017118437727	2.4806442143558824E-4	8.133259719199614E-6	2.999139521454858E-4	2.236646422779894E-4
88	1.499244430338661	2.4806442143558824E-4	8.133259719199614E-6	3.0499723946998556E-4	2.2976458706738912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	42095	0.0	65.81197	2
ATATCTC	45465	0.0	61.696644	1
ATCTCGA	59330	0.0	46.310463	3
TATGGAT	53970	0.0	44.27582	2
ATATGGA	57665	0.0	41.908905	1
TCTCGAG	68380	0.0	39.857952	4
CTCGAGG	78290	0.0	34.694492	5
ATGGATC	73140	0.0	32.829544	3
GGATCCG	72205	0.0	32.47584	5
TGGATCC	82425	0.0	28.999989	4
TCGAGGG	106015	0.0	25.563635	6
GATCCGG	94885	0.0	24.748665	6
CGAGGGC	122035	0.0	22.154133	7
ATCCGGC	105050	0.0	22.067842	7
AGGGCGC	131705	0.0	20.463646	9
GAGGGCG	136130	0.0	19.850044	8
TCCGGCG	117440	0.0	19.667763	8
GCGCCGT	123900	0.0	13.260259	9
CCGGCGC	177000	0.0	12.967441	9
CGTCGAC	121785	0.0	12.21179	12-13
>>END_MODULE