FastQCFastQC Report
Wed 25 May 2016
SRR445725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51954904
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1720200.3310948279300064No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1538450.29611256716016643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610600.11752499821768508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG493950.072.730092
ATATCTC550000.065.958181
ATCTCGA699850.051.1002433
ATATGGA601000.050.941141
TATGGAT602550.050.532512
TCTCGAG777400.045.549784
CTCGAGG896350.039.3582275
ATGGATC906950.033.606023
GGATCCG919700.032.552745
TGGATCC1011500.030.025954
TCGAGGG1220300.028.9529236
CGAGGGC1317550.026.7443547
GATCCGG1153900.025.9516096
GAGGGCG1430200.024.6016128
ATCCGGC1210150.024.512167
AGGGCGC1452150.024.119769
TCCGGCG1331300.022.2567448
CCGGCGC2063000.014.4903729
CCGTCGA1354550.013.08876316-17
CGCCGTC1398750.012.82800814-15
Produced by FastQC (version 0.11.5)