##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445725_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51954904
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4364695197974	33.0	31.0	34.0	29.0	34.0
2	31.703716149682425	34.0	31.0	34.0	30.0	34.0
3	31.80624968530401	34.0	31.0	34.0	30.0	34.0
4	35.18433081889633	37.0	35.0	37.0	32.0	37.0
5	34.940393711438674	37.0	35.0	37.0	32.0	37.0
6	34.88377884405291	37.0	35.0	37.0	32.0	37.0
7	34.83711847489892	37.0	35.0	37.0	32.0	37.0
8	34.79826739743374	37.0	35.0	37.0	32.0	37.0
9	36.39993258384233	39.0	37.0	39.0	32.0	39.0
10-11	36.33485052729575	39.0	37.0	39.0	32.0	39.0
12-13	36.242919503806604	39.0	37.0	39.0	32.0	39.0
14-15	37.48333242998582	40.0	38.0	41.0	32.0	41.0
16-17	37.41603814723631	40.0	38.0	41.0	32.0	41.0
18-19	37.33677859360495	40.0	38.0	41.0	31.5	41.0
20-21	37.24773468929901	40.0	38.0	41.0	31.0	41.0
22-23	37.12318985326198	40.0	37.5	41.0	31.0	41.0
24-25	36.96607368382396	40.0	37.0	41.0	31.0	41.0
26-27	36.729191001873474	40.0	37.0	41.0	30.0	41.0
28-29	36.45222824394017	40.0	36.0	41.0	30.0	41.0
30-31	36.20285768404076	39.5	35.5	41.0	30.0	41.0
32-33	35.968150071069324	39.0	35.0	41.0	29.0	41.0
34-35	35.864646646253064	39.0	35.0	41.0	28.5	41.0
36-37	35.66311204232039	39.0	35.0	41.0	27.0	41.0
38-39	35.454592063147686	39.0	35.0	41.0	26.0	41.0
40-41	35.33752813786356	39.0	35.0	41.0	25.0	41.0
42-43	35.03993137009742	39.0	35.0	41.0	23.5	41.0
44-45	34.685260769608966	39.0	35.0	41.0	21.0	41.0
46-47	34.30153976417702	39.0	34.5	41.0	16.5	41.0
48-49	33.854319392063545	39.0	34.0	41.0	8.0	41.0
50-51	33.51017050286533	39.0	33.0	41.0	2.0	41.0
52-53	33.177314830569216	38.5	33.0	41.0	2.0	41.0
54-55	32.83778894096311	38.0	33.0	40.5	2.0	41.0
56-57	32.431724414311304	38.0	32.0	40.0	2.0	41.0
58-59	32.044473799816856	37.0	31.5	40.0	2.0	41.0
60-61	31.67093907054472	37.0	31.0	40.0	2.0	41.0
62-63	31.259928985721928	36.0	31.0	40.0	2.0	41.0
64-65	30.792528930474013	35.5	30.0	39.5	2.0	41.0
66-67	30.325559171469166	35.0	29.5	39.0	2.0	41.0
68-69	29.850440961261327	35.0	29.0	39.0	2.0	41.0
70-71	29.33152306469472	35.0	28.5	38.0	2.0	40.0
72-73	28.796997575050856	35.0	27.5	37.0	2.0	40.0
74-75	28.23622702680771	34.0	26.0	37.0	2.0	39.0
76-77	27.485048013946866	33.5	25.5	36.0	2.0	39.0
78-79	27.438221365975387	34.0	25.5	36.0	2.0	38.5
80-81	27.063719057203915	34.0	24.5	35.0	2.0	37.0
82-83	26.571007079524197	34.0	23.5	35.0	2.0	37.0
84-85	26.07295906080396	34.0	20.0	35.0	2.0	36.5
86-87	25.60525429899745	33.0	17.5	35.0	2.0	36.0
88-89	25.0924427461169	33.0	8.5	35.0	2.0	36.0
90-91	24.587979538947852	33.0	2.0	35.0	2.0	35.0
92-93	23.959382727374496	32.5	2.0	35.0	2.0	35.0
94-95	23.31169933448438	32.0	2.0	35.0	2.0	35.0
96-97	22.72962877575522	32.0	2.0	35.0	2.0	35.0
98-99	22.03078014541226	31.0	2.0	35.0	2.0	35.0
100	20.92884365641403	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12761740523675158
1101	2	0.11245633978291636
1101	3	0.14170589305543402
1101	4	0.13460591761836582
1101	5	0.19718808180756042
1101	6	0.1906970531995853
1101	7	0.21069942741550562
1101	8	0.20865277410479166
1101	9	0.23707229807760655
1101	10-11	0.22391717283188228
1101	12-13	0.2607643023809203
1101	14-15	0.2550239535297507
1101	16-17	0.25006421934949685
1101	18-19	0.2225310051002083
1101	20-21	0.20839912992632748
1101	22-23	0.23635079889198352
1101	24-25	0.2238234651600095
1101	26-27	0.24467604861614234
1101	28-29	0.22261686611813758
1101	30-31	0.18590530362168067
1101	32-33	0.15550457603846723
1101	34-35	0.17079204921643054
1101	36-37	0.16883150492994048
1101	38-39	0.12790109764220148
1101	40-41	0.11472404606051612
1101	42-43	0.13081372507819822
1101	44-45	0.0731184231776254
1101	46-47	0.0700403921185142
1101	48-49	0.05479817036673751
1101	50-51	0.036091435956066675
1101	52-53	0.01211204627418283
1101	54-55	-0.003502582506314411
1101	56-57	0.006644294041883825
1101	58-59	0.008711756304307983
1101	60-61	-0.03320852771312488
1101	62-63	-0.05434186091879312
1101	64-65	-0.10052922573233047
1101	66-67	-0.09418494921186493
1101	68-69	-0.02236165155601455
1101	70-71	-0.07572885539112661
1101	72-73	-0.07568060361131401
1101	74-75	-0.20726814315595732
1101	76-77	-0.09471422968222853
1101	78-79	-0.08972644755339942
1101	80-81	-0.12736383657451356
1101	82-83	-0.21187727478712048
1101	84-85	-0.08405883730677388
1101	86-87	-0.24062496560484448
1101	88-89	-0.2404896253012474
1101	90-91	-0.28311705542412824
1101	92-93	-0.36424932573907043
1101	94-95	-0.1488265881387747
1101	96-97	-0.3306610130705998
1101	98-99	-0.2260970712820054
1101	100	-0.18974041459991042
1102	1	0.09455188926600755
1102	2	0.09023121897205755
1102	3	0.10490361159770956
1102	4	0.10602753238759988
1102	5	0.1230102783119591
1102	6	0.17084357880316503
1102	7	0.16846076965402546
1102	8	0.16353032359543818
1102	9	0.17110037338527206
1102	10-11	0.1948235324965637
1102	12-13	0.17791473056088591
1102	14-15	0.17135729566646063
1102	16-17	0.10331684805802865
1102	18-19	0.14912355177658299
1102	20-21	0.1622149421189576
1102	22-23	0.17904401267583125
1102	24-25	0.1843170805733081
1102	26-27	0.18200996376392453
1102	28-29	0.16823417147528374
1102	30-31	0.17859240045942215
1102	32-33	0.09921888548795721
1102	34-35	0.10777956908157194
1102	36-37	0.13924885963838562
1102	38-39	0.04939909442104096
1102	40-41	0.09065830870731162
1102	42-43	0.12149871267482837
1102	44-45	0.1064858357461489
1102	46-47	0.09810780546728637
1102	48-49	0.0914787590894619
1102	50-51	0.06612812038721927
1102	52-53	0.02929463431728152
1102	54-55	-0.028747538647166948
1102	56-57	0.041514260367101485
1102	58-59	-0.03314664539959722
1102	60-61	-0.014379362660658757
1102	62-63	-0.027302343426928388
1102	64-65	-0.029610098338743285
1102	66-67	-0.09370091961110916
1102	68-69	-0.015170504912497051
1102	70-71	-0.026373790511804884
1102	72-73	0.005446290938866838
1102	74-75	-0.1604181289881268
1102	76-77	-0.024820604056007056
1102	78-79	-0.030415931493358528
1102	80-81	-0.055056377602365814
1102	82-83	-0.15390851540067985
1102	84-85	-0.029818883521723905
1102	86-87	-0.17762515836494686
1102	88-89	-0.2228884439765082
1102	90-91	-0.2946787389546799
1102	92-93	-0.36637142685248847
1102	94-95	-0.1482528842613533
1102	96-97	-0.30109684919210267
1102	98-99	-0.17423179704397285
1102	100	-0.1401203384350289
1103	1	0.06887484603598182
1103	2	0.07164938754914374
1103	3	0.09249604915732235
1103	4	0.11177275633581729
1103	5	0.12096044428649577
1103	6	0.1388515889021278
1103	7	0.09748690816201844
1103	8	0.11901269752105037
1103	9	0.16289471909352926
1103	10-11	0.10710906574802692
1103	12-13	0.13975214637056865
1103	14-15	0.10085433980520975
1103	16-17	0.10711483567898483
1103	18-19	0.11230151875713545
1103	20-21	0.06076349525913116
1103	22-23	0.060211792008416865
1103	24-25	0.12538801577731817
1103	26-27	-0.01988552996996873
1103	28-29	0.0791428885556158
1103	30-31	0.10043137397326518
1103	32-33	0.04832875678779658
1103	34-35	0.06743491815241498
1103	36-37	0.07805118955975843
1103	38-39	0.052157167965354745
1103	40-41	-0.01437277755892552
1103	42-43	-0.024029645040833714
1103	44-45	0.0057121736832144165
1103	46-47	-0.06215321683894359
1103	48-49	0.013536326267832521
1103	50-51	-0.02677160512180876
1103	52-53	-0.013449714600085372
1103	54-55	0.04277646709255123
1103	56-57	0.0498070759319873
1103	58-59	-0.13925862335319295
1103	60-61	-0.13491008827254092
1103	62-63	-0.15751757945064426
1103	64-65	-0.22738526129904812
1103	66-67	-0.08071308291247803
1103	68-69	-0.04396256459372694
1103	70-71	-0.05420394343001078
1103	72-73	-0.034707673803083594
1103	74-75	-0.11487039875107286
1103	76-77	-0.031785498403170465
1103	78-79	-0.05755523275758634
1103	80-81	-0.08101953605324397
1103	82-83	-0.07895353312207476
1103	84-85	0.009009807195489827
1103	86-87	-0.1903696726954962
1103	88-89	-0.20719972895852834
1103	90-91	-0.27356331441945514
1103	92-93	-0.28006205412211216
1103	94-95	-0.20675160135499482
1103	96-97	-0.31947905668353727
1103	98-99	-0.18270385375290488
1103	100	-0.17233764794367445
1104	1	0.005584201114256615
1104	2	-0.012514759277198806
1104	3	-0.008918336840277874
1104	4	-0.020367277865148026
1104	5	0.0017429513981070954
1104	6	0.055140880476152176
1104	7	0.04814158950053837
1104	8	0.03406043967687822
1104	9	0.07017808430228456
1104	10-11	0.07152436518914129
1104	12-13	0.05325712772071256
1104	14-15	0.06947991910784879
1104	16-17	0.05938411253444542
1104	18-19	0.049356854059539046
1104	20-21	0.07515075191506781
1104	22-23	-0.004279265262418619
1104	24-25	0.04454539029370608
1104	26-27	0.07047430768545127
1104	28-29	0.03838651410674032
1104	30-31	0.032809422520685416
1104	32-33	0.004009850679608462
1104	34-35	0.04673810419923541
1104	36-37	0.06680521246454418
1104	38-39	0.06568843669344204
1104	40-41	0.008410975423558398
1104	42-43	0.07530781079628213
1104	44-45	0.0677120707207024
1104	46-47	0.04716212477193693
1104	48-49	0.06860917774701392
1104	50-51	0.036125978106419154
1104	52-53	0.05052190919688826
1104	54-55	0.0036441878994324384
1104	56-57	0.004698323478230293
1104	58-59	-0.06811918527004224
1104	60-61	-0.08668798602668915
1104	62-63	-0.10244482686094258
1104	64-65	-0.07176808302395443
1104	66-67	-0.09093951023791647
1104	68-69	-0.008007576285763207
1104	70-71	-0.05411251352331803
1104	72-73	0.06651443049992167
1104	74-75	-0.10756371705498324
1104	76-77	-0.03280347586204613
1104	78-79	0.008277958019345277
1104	80-81	-0.010953211469875157
1104	82-83	-0.07668001005098546
1104	84-85	0.05606255481589173
1104	86-87	-0.07777008226427284
1104	88-89	-0.10716910734855745
1104	90-91	-0.137367932147761
1104	92-93	-0.169034276255978
1104	94-95	-0.061626763964689246
1104	96-97	-0.15764556602863777
1104	98-99	-0.07773977506093388
1104	100	-0.15710665279752334
1105	1	-0.03148202736041483
1105	2	-0.027934337242736973
1105	3	-0.013832754215247434
1105	4	-0.01702318391197366
1105	5	0.01606773959291985
1105	6	0.00540069914789143
1105	7	0.029388100103084014
1105	8	0.01630433835337186
1105	9	0.050546516200824954
1105	10-11	0.008388456705134217
1105	12-13	0.05799725444010306
1105	14-15	0.059523317600373105
1105	16-17	0.06305540195511838
1105	18-19	0.02789293877272314
1105	20-21	0.047949406410872086
1105	22-23	0.06057317353550218
1105	24-25	0.04661817848430161
1105	26-27	0.04726366668720061
1105	28-29	-0.052288168888487974
1105	30-31	0.015192860091154614
1105	32-33	0.058685557618147755
1105	34-35	0.05195972944732574
1105	36-37	0.057712436699269176
1105	38-39	0.03985813505473601
1105	40-41	0.048579069047498535
1105	42-43	0.04659901712673076
1105	44-45	0.08079023884145187
1105	46-47	0.008262518671465102
1105	48-49	0.049732396542758295
1105	50-51	0.09702765268487923
1105	52-53	0.13345060880838844
1105	54-55	0.05229915447438316
1105	56-57	0.08511287540851953
1105	58-59	0.11632582016252258
1105	60-61	0.11404741818552466
1105	62-63	0.03369272468889406
1105	64-65	-0.038711774387387266
1105	66-67	-0.04112067614630632
1105	68-69	0.028756581008057225
1105	70-71	0.07014081417498375
1105	72-73	0.12126216030638304
1105	74-75	-0.010313036617532134
1105	76-77	0.05801309631466367
1105	78-79	0.08229053333803549
1105	80-81	0.11961679482275045
1105	82-83	0.06735707700548588
1105	84-85	0.1190159512020692
1105	86-87	0.0055139558722459014
1105	88-89	0.008021643068833129
1105	90-91	-0.05244170693112693
1105	92-93	-0.14091785159966008
1105	94-95	-0.052500572344062135
1105	96-97	-0.08929962316091178
1105	98-99	0.027685348485469774
1105	100	-0.024622615930294245
1106	1	-0.18196326181397993
1106	2	-0.15747333559058063
1106	3	-0.11254712026800107
1106	4	-0.10086061732535256
1106	5	-0.13328855422369656
1106	6	-0.1886062862907778
1106	7	-0.19552769501097345
1106	8	-0.3009862250189599
1106	9	-0.21919045090688627
1106	10-11	-0.19082889743021525
1106	12-13	-0.16348849536785792
1106	14-15	-0.16510910831063086
1106	16-17	-0.1542071355345911
1106	18-19	-0.16824772532666543
1106	20-21	-0.31812621598443513
1106	22-23	-0.1875966359701522
1106	24-25	-0.1542245191028826
1106	26-27	-0.17679186394536828
1106	28-29	-0.2324346767965224
1106	30-31	-0.17460879872704993
1106	32-33	-0.1928611324740217
1106	34-35	-0.16677204137840818
1106	36-37	-0.1725572458198954
1106	38-39	-0.19404749919271325
1106	40-41	-0.22080395709209455
1106	42-43	-0.17303654102834543
1106	44-45	-0.19325886917558677
1106	46-47	-0.19738944233497335
1106	48-49	-0.14780194585122075
1106	50-51	-0.19673572643809223
1106	52-53	-0.15677963701796926
1106	54-55	-0.18667337305211618
1106	56-57	-0.1590877396738577
1106	58-59	-0.15811127229373056
1106	60-61	-0.32554201892417467
1106	62-63	-0.2286983076588598
1106	64-65	-0.2799547455728515
1106	66-67	-0.2724792394287192
1106	68-69	-0.2775094364997308
1106	70-71	-0.16180027131756702
1106	72-73	-0.2061947965384583
1106	74-75	-0.30193655198391767
1106	76-77	-0.2489881318374536
1106	78-79	-0.21886677299523072
1106	80-81	-0.1919492678931647
1106	82-83	-0.20293812840873926
1106	84-85	-0.15056788009682975
1106	86-87	-0.2522875886368787
1106	88-89	-0.14958172190256036
1106	90-91	-0.26930924323712446
1106	92-93	-0.3581841466654154
1106	94-95	-0.3295890220132307
1106	96-97	-0.3073392861312776
1106	98-99	-0.10346273850917242
1106	100	-0.2128259296897781
1107	1	-0.154160496030034
1107	2	-0.1698881985052232
1107	3	-0.13863782860520857
1107	4	-0.10145625795757951
1107	5	-0.15466673181929735
1107	6	-0.20958069617304176
1107	7	-0.17305870732956663
1107	8	-0.18065079264028583
1107	9	-0.20212018696729928
1107	10-11	-0.11901573356158934
1107	12-13	-0.1367348327567015
1107	14-15	-0.21984740602344743
1107	16-17	-0.19418778387282032
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2207	62-63	0.10519050543130248
2207	64-65	0.08868140490771736
2207	66-67	0.04840524985943162
2207	68-69	0.02501432280028837
2207	70-71	0.04394205015617558
2207	72-73	0.04139477204977737
2207	74-75	0.1400807348553279
2207	76-77	0.0857216917722674
2207	78-79	0.12098127654273227
2207	80-81	0.05213768293556953
2207	82-83	0.13010013416432997
2207	84-85	0.15711396473080796
2207	86-87	0.1762076765348688
2207	88-89	0.16277272719284142
2207	90-91	0.19359782218459287
2207	92-93	0.3000394507492672
2207	94-95	0.331304348063469
2207	96-97	0.4496840662543171
2207	98-99	0.4194658020209161
2207	100	0.323971513997229
2208	1	-0.27335982438990314
2208	2	-0.2550055499926067
2208	3	-0.25479366424282546
2208	4	-0.30635408904453953
2208	5	-0.3260047137163582
2208	6	-0.3423837696049574
2208	7	-0.3276486848522637
2208	8	-0.29364369169618243
2208	9	-0.32615869559195687
2208	10-11	-0.34364196511800316
2208	12-13	-0.309836798399985
2208	14-15	-0.3453253712658153
2208	16-17	-0.309828377595025
2208	18-19	-0.38709766044767946
2208	20-21	-0.35316366536579125
2208	22-23	-0.3766961207878978
2208	24-25	-0.38747895980760916
2208	26-27	-0.34756927527119785
2208	28-29	-0.34188649393066584
2208	30-31	-0.3215323604460991
2208	32-33	-0.26410733291767485
2208	34-35	-0.33416066172638637
2208	36-37	-0.26899166967058363
2208	38-39	-0.2265316410520981
2208	40-41	-0.32816700607045846
2208	42-43	-0.22815640888807565
2208	44-45	-0.21801232567678852
2208	46-47	-0.18842159745207
2208	48-49	-0.22009488020264456
2208	50-51	-0.14934690191613242
2208	52-53	-0.13495350930062955
2208	54-55	-0.1522118231490026
2208	56-57	-1.3293356841637731
2208	58-59	-0.8436775460956198
2208	60-61	-0.38638682267322366
2208	62-63	-0.018090123913445666
2208	64-65	0.1448857929682461
2208	66-67	0.05646271350637733
2208	68-69	0.11558061041480627
2208	70-71	0.15386701611172526
2208	72-73	0.18062577531642532
2208	74-75	0.22806872832208214
2208	76-77	0.1749307225363559
2208	78-79	0.2918220771441362
2208	80-81	0.3027278076959625
2208	82-83	0.4352177634930321
2208	84-85	0.41200681829372954
2208	86-87	0.4873186145959423
2208	88-89	0.4591213935660967
2208	90-91	0.487928503604536
2208	92-93	0.6423214901910548
2208	94-95	-0.030039881573841853
2208	96-97	0.6635557136805517
2208	98-99	0.6712749721152633
2208	100	-0.18348179790508112
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	859517.0
3	278934.0
4	169839.0
5	141029.0
6	139189.0
7	155145.0
8	175536.0
9	194408.0
10	211610.0
11	220446.0
12	237138.0
13	291513.0
14	419434.0
15	625694.0
16	720056.0
17	707193.0
18	696855.0
19	558511.0
20	475667.0
21	480202.0
22	507455.0
23	551198.0
24	608327.0
25	681491.0
26	770001.0
27	868545.0
28	985719.0
29	1121761.0
30	1293859.0
31	1499573.0
32	1764843.0
33	2151002.0
34	2739409.0
35	3684837.0
36	5348079.0
37	8021552.0
38	9135332.0
39	2460455.0
40	3550.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.98427477737169	28.320001802030927	27.033721370017112	22.662002050580274
2	23.404363729418122	26.914072187046422	28.293519387569738	21.388044695965718
3	23.424041873854367	27.129980920760854	26.84839247699861	22.59758472838617
4	23.941269894164673	26.002431073993094	27.441454695599532	22.6148443362427
5	24.756958218489682	26.13827404973567	25.802239594378307	23.30252813739634
6	24.889261803423544	25.913175910401186	25.864418554089337	23.333143732085933
7	24.123607339084703	26.60192902117531	25.50433085147122	23.770132788268768
8	24.375162058184706	25.819007490858382	26.03879741513791	23.767033035819
9	24.20168125781201	26.16137463356599	25.603011746060638	24.03393236256136
10-11	24.744476691297347	25.8068128333509	25.566804804402953	23.881905670948797
12-13	24.11288552613156	25.928287873011456	25.816519129615518	24.142307471241466
14-15	23.684274833711065	26.044424119041203	26.342520479976685	23.928780567271048
16-17	23.50111968656091	26.105334615717616	26.679218009935717	23.71432768778575
18-19	23.027983938287825	26.110116460069317	27.582892100463514	23.279007501179343
20-21	22.43227674004436	26.22647968858476	28.709965838963384	22.631277732407497
22-23	22.165991768399007	26.117370449821752	29.304907431784805	22.411730349994443
24-25	21.87410761758395	25.78539878423054	30.070172157894792	22.270321440290722
26-27	21.827281382759363	25.844354304774065	30.715295637712074	21.613068674754498
28-29	21.814478946656454	25.970002749353714	30.679411421189517	21.53610688280031
30-31	21.766527374428865	26.032704479556983	30.5992065926351	21.60156155337905
32-33	21.790063289598795	26.1266696823773	30.506665852605085	21.576601175418823
34-35	21.78517692870771	26.254734006817483	30.450806086930932	21.50928297754388
36-37	21.782250179916602	26.34084604539141	30.3148574429277	21.562046331764297
38-39	21.86279689024742	26.484676919869322	30.082697286482833	21.569828903400424
40-41	21.906141568223404	26.79845561742124	29.660193891175595	21.635208923179757
42-43	22.0451749433431	27.094230401167174	29.068900283247697	21.791694372242034
44-45	22.23082942544363	27.512775436037177	28.403549266820853	21.85284587169833
46-47	22.29961688980274	27.87963625801258	27.792627529998875	22.028119322185802
48-49	22.53919998061184	28.165080449654802	27.112523913613078	22.183195656120283
50-51	22.577481041468143	28.552147472258472	26.547329485338288	22.323042000935096
52-53	22.615067261005635	28.767071500584496	26.26352619097086	22.354335047439015
54-55	22.680078655680582	28.872150408473686	26.08444518111143	22.363325754734305
56-57	22.638708944998974	29.11405058555553	25.90593105521048	22.341309414235017
58-59	22.640490385663988	29.282788335286597	25.773398135050584	22.30332314399883
60-61	22.623817287100174	29.480566895995047	25.598348696242283	22.297267120662497
62-63	22.63891409426473	29.643173895838	25.519733566309945	22.198178443587327
64-65	22.652169120645045	29.873392737764114	25.336382360459037	22.1380557811318
66-67	22.646315491823326	30.040351919793174	25.166748769300177	22.146583819083325
68-69	22.62419683733068	30.25845803449941	24.995435177279834	22.121909950890082
70-71	22.62639797470397	30.470753287006648	24.822401198905894	22.080447539383492
72-73	22.60078755544785	30.653618281264727	24.654817356593732	22.090776806693686
74-75	22.65078250547847	30.815686284624537	24.526125351897882	22.007405857999107
76-77	22.382270923232447	31.20970810261009	24.255264370357693	22.15275660379977
78-79	22.378369249756663	31.40438482518551	24.05414510877835	22.163100816279478
80-81	22.388687100139755	31.6067055920986	23.8944073836413	22.110199924120344
82-83	22.35208900872565	31.82285312639261	23.71424090817248	22.110816956709257
84-85	22.307894393094614	32.029772003867215	23.462633303034046	22.199700300004128
86-87	22.357291380376545	32.18801468459229	23.26664440958477	22.18804952544639
88-89	22.353466934370744	32.34599136447235	23.042365810276937	22.25817589087997
90-91	22.380487935198712	32.47510475484472	22.81594961766375	22.328457692292815
92-93	22.37278417636488	32.66895858381237	22.527900360379903	22.430356879442847
94-95	22.41348724090484	32.78459009891644	22.326491616585802	22.475431043592923
96-97	22.393528358097466	32.91642852608374	22.05879659106699	22.631246524751806
98-99	22.415520879277253	33.05373251461787	21.87947183181015	22.651274774294734
100	22.911383025820086	32.77892671422224	21.141731712764088	23.167958547193592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7818.0
1	5389.0
2	2710.5
3	2479.0
4	2637.5
5	2939.0
6	3421.5
7	4143.5
8	4972.5
9	6076.0
10	7504.5
11	9327.5
12	12291.5
13	17503.5
14	26395.0
15	37119.5
16	67750.5
17	80923.0
18	75412.5
19	91232.5
20	119399.5
21	148443.0
22	176135.5
23	215573.5
24	262242.0
25	316049.0
26	376874.5
27	443227.5
28	521503.0
29	604999.5
30	679986.5
31	759548.5
32	851861.5
33	952195.0
34	1059092.5
35	1178181.0
36	1308107.5
37	1426769.5
38	1540508.5
39	1633323.5
40	1689705.0
41	1735644.5
42	1787942.0
43	1866321.5
44	1959160.5
45	1996963.0
46	2006892.5
47	1992880.0
48	1917913.0
49	1832775.5
50	1751359.5
51	1685987.5
52	1609518.0
53	1490915.5
54	1359712.5
55	1237960.0
56	1116778.0
57	1006478.5
58	930002.0
59	855976.0
60	752768.5
61	658019.5
62	574677.0
63	490252.0
64	416750.0
65	352564.5
66	299663.0
67	249265.5
68	202762.0
69	167222.5
70	141734.5
71	125011.0
72	113447.5
73	104710.0
74	95313.0
75	78439.5
76	62901.0
77	54961.0
78	48446.5
79	38318.5
80	25339.5
81	14812.0
82	8145.5
83	4166.0
84	2294.5
85	1381.5
86	896.5
87	593.5
88	368.0
89	220.0
90	133.5
91	91.0
92	60.5
93	45.0
94	30.5
95	23.0
96	15.0
97	7.5
98	5.0
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026001395363948705
2	0.009413933283372056
3	3.849492244273995E-6
4	0.019172396122606634
5	0.024846547690666507
6	0.0072543681343343455
7	0.005098652477540907
8	0.004066988556075476
9	0.00548167695584617
10-11	0.005566365785220198
12-13	0.007479563430624374
14-15	0.008831697581425615
16-17	0.007233195926990838
18-19	0.006556647665059683
20-21	0.004134354670350272
22-23	0.01076510506111223
24-25	0.005834867869258309
26-27	9.046306774043889E-4
28-29	0.00168607760299201
30-31	0.003889911912838873
32-33	0.0027379513587398796
34-35	0.005537494593388143
36-37	0.004543363221304384
38-39	0.002783182892610099
40-41	0.00305168497664821
42-43	0.003103653121945909
44-45	0.008143600842761637
46-47	0.00730248678738777
48-49	0.010923896616188531
50-51	0.00760948379386862
52-53	0.00677029448461689
54-55	0.011949786299287552
56-57	0.020154016644896505
58-59	0.025355643039971745
60-61	0.037914611486915654
62-63	0.028728760619016833
64-65	0.0041526397585105726
66-67	0.0011981544610302811
68-69	0.0010230025639158143
70-71	0.004239253334006738
72-73	0.00456453542864789
74-75	0.003805223083464845
76-77	0.0020498546200759028
78-79	0.001348284658556967
80-81	5.148695876716469E-4
82-83	0.0017255348984958187
84-85	0.008249461879479173
86-87	0.008465033445158518
88-89	0.005797335319876638
90-91	9.719967916791839E-4
92-93	0.006160149963899461
94-95	0.00883458470060882
96-97	0.022464674364521972
98-99	0.014458692869493127
100	0.016928142144194896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.1954904E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.91849365480761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76484207817315	31.75948051911402
2	11.835511666722939	9.922536414058541
3	4.324171174151858	5.4378822577795995
4	2.123210215767061	3.5600709582981698
5	1.3067243851771722	2.738795892431584
6	0.8842647416174589	2.224022757639693
7	0.5858434483646574	1.7190372411089152
8	0.457888595300434	1.5355200141368013
9	0.3454857113099766	1.3034016537636552
>10	2.0375913727779134	15.745480431408469
>50	0.210561471433173	6.244171727148451
>100	0.10896094826357593	7.842308521362576
>500	0.006929823884793038	2.012895634380946
>1k	0.007798204569852801	6.004949616289984
>5k	8.738506845768179E-5	0.2419230655685451
>10k+	1.2877741747026737E-4	1.70752329551014
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	172020	0.3310948279300064	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	153845	0.29611256716016643	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61060	0.11752499821768508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.924746122136998E-6	0.0	1.924746122136998E-6	1.924746122136998E-6
2	0.0	7.698984488547992E-6	0.0	9.62373061068499E-6	5.774238366410993E-6
3	0.0	1.732271509923298E-5	0.0	1.3473222854958985E-5	5.774238366410993E-6
4	0.0	2.1172207343506978E-5	0.0	1.5397968977095984E-5	9.62373061068499E-6
5	5.774238366410993E-6	2.694644570991797E-5	0.0	1.924746122136998E-5	1.5397968977095984E-5
6	7.698984488547992E-6	2.887119183205497E-5	0.0	1.924746122136998E-5	1.924746122136998E-5
7	1.1548476732821987E-5	2.887119183205497E-5	0.0	2.5021699587780972E-5	2.3096953465643973E-5
8	1.1548476732821987E-5	3.657017632060296E-5	0.0	2.887119183205497E-5	2.3096953465643973E-5
9	3.079593795419197E-5	3.657017632060296E-5	1.924746122136998E-6	3.464543019846596E-5	2.694644570991797E-5
10-11	9.33501869236444E-5	3.7532549381671455E-5	1.924746122136998E-6	4.138204162594545E-5	2.887119183205497E-5
12-13	2.935237836258922E-4	4.0419668564876956E-5	1.924746122136998E-6	4.330678774808246E-5	3.079593795419197E-5
14-15	6.342038472441408E-4	4.6193906931287946E-5	1.924746122136998E-6	4.8118653053424945E-5	3.1758311015260465E-5
16-17	0.0011404120773661711	5.2930518358767446E-5	1.924746122136998E-6	5.100577223663044E-5	3.368305713739746E-5
18-19	0.0019064610339766964	5.8704756725178436E-5	1.924746122136998E-6	5.774238366410994E-5	3.657017632060296E-5
20-21	0.0028851944370833597	6.159187590838394E-5	1.924746122136998E-6	6.255424896945243E-5	3.7532549381671455E-5
22-23	0.004296995717670848	6.544136815265792E-5	1.924746122136998E-6	6.929086039693192E-5	4.330678774808246E-5
24-25	0.006206343870830749	7.217797958013742E-5	1.924746122136998E-6	7.795221794654842E-5	4.908102611449345E-5
26-27	0.008512189725150873	7.602747182441142E-5	1.924746122136998E-6	8.66135754961649E-5	5.389289141983594E-5
28-29	0.013439539797821587	7.987696406868542E-5	1.924746122136998E-6	9.335018692364439E-5	5.6780010603041443E-5
30-31	0.017478619535126076	8.757594855723341E-5	1.924746122136998E-6	9.912442529005539E-5	6.159187590838394E-5
32-33	0.018671962130851018	9.335018692364439E-5	1.924746122136998E-6	1.0586103671753489E-4	6.544136815265792E-5
34-35	0.020025058654713324	9.623730610684989E-5	1.924746122136998E-6	1.1933425957249386E-4	6.929086039693192E-5
36-37	0.02147727960386569	1.0104917141219238E-4	1.924746122136998E-6	1.2703324406104186E-4	6.929086039693192E-5
38-39	0.023034399216674523	1.020115444732609E-4	1.924746122136998E-6	1.3184510936638434E-4	6.929086039693192E-5
40-41	0.024854246675155053	1.0586103671753489E-4	1.924746122136998E-6	1.4146883997706935E-4	7.217797958013742E-5
42-43	0.027078290819284354	1.0874815590074038E-4	1.924746122136998E-6	1.5013019752668585E-4	8.37264563129594E-5
44-45	0.029734440467833412	1.1548476732821988E-4	1.924746122136998E-6	1.6167867425950783E-4	9.431255998471288E-5
46-47	0.033163375684420474	1.1740951345035687E-4	1.924746122136998E-6	1.684152856869873E-4	9.912442529005539E-5
48-49	0.03739108054169439	1.1837188651142537E-4	1.924746122136998E-6	1.799637624198093E-4	1.020115444732609E-4
50-51	0.04280154189102149	1.2222137875569938E-4	1.924746122136998E-6	1.905498660915628E-4	1.020115444732609E-4
52-53	0.049547777049111674	1.2895799018317885E-4	1.924746122136998E-6	1.972864775190423E-4	1.020115444732609E-4
54-55	0.058384286495842624	1.3280748242745283E-4	1.924746122136998E-6	2.040230889465218E-4	1.0297391753432939E-4
56-57	0.069615180118512	1.3954409385493236E-4	1.924746122136998E-6	2.040230889465218E-4	1.0393629059539789E-4
58-59	0.08367063867541744	1.4628070528241183E-4	5.774238366410993E-6	2.0787258119079577E-4	1.0393629059539789E-4
60-61	0.1009846924171008	1.5013019752668582E-4	5.774238366410993E-6	2.1172207343506978E-4	1.0778578283967188E-4
62-63	0.12270352765929468	1.5494206283202833E-4	5.774238366410993E-6	2.1653393874041226E-4	1.0874815590074038E-4
64-65	0.14938917026966309	1.645657934427133E-4	5.774238366410993E-6	2.1845868486254925E-4	1.1259764814501438E-4
66-67	0.1824688194977706	1.722647779312613E-4	5.774238366410993E-6	2.2423292322896023E-4	1.1452239426715138E-4
68-69	0.2235140305523421	1.809261354808778E-4	5.774238366410993E-6	2.3770614608391923E-4	1.1644714038928837E-4
70-71	0.2729318872382095	1.876627469083573E-4	5.774238366410993E-6	2.4444275751139876E-4	1.1933425957249386E-4
72-73	0.33247679564570076	1.9921122364117928E-4	5.774238366410993E-6	2.4925462281674124E-4	1.2222137875569938E-4
74-75	0.4040311574822658	2.0594783506865878E-4	5.774238366410993E-6	2.559912342442207E-4	1.2895799018317885E-4
76-77	0.48942540631005693	2.1364681955720677E-4	7.698984488547992E-6	2.6272784567170024E-4	1.3473222854958985E-4
78-79	0.5898105403101119	2.2615766935109725E-4	7.698984488547992E-6	2.713892032213167E-4	1.3858172079386387E-4
80-81	0.7054723842815684	2.3289428077857675E-4	7.698984488547992E-6	2.790881877098647E-4	1.4820545140454883E-4
82-83	0.8384887016632732	2.3578139996178224E-4	7.698984488547992E-6	2.848624260762757E-4	1.5782918201523384E-4
84-85	0.9893291305090276	2.4636750363353575E-4	7.698984488547992E-6	2.867871721984127E-4	1.703400318091243E-4
86-87	1.1605670563841288	2.5021699587780973E-4	7.698984488547992E-6	2.896742913816182E-4	1.9054986609156282E-4
88	1.3040231967323046	2.5021699587780973E-4	7.698984488547992E-6	2.9448615668696066E-4	1.963241044579738E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	49395	0.0	72.73009	2
ATATCTC	55000	0.0	65.95818	1
ATCTCGA	69985	0.0	51.100243	3
ATATGGA	60100	0.0	50.94114	1
TATGGAT	60255	0.0	50.53251	2
TCTCGAG	77740	0.0	45.54978	4
CTCGAGG	89635	0.0	39.358227	5
ATGGATC	90695	0.0	33.60602	3
GGATCCG	91970	0.0	32.55274	5
TGGATCC	101150	0.0	30.02595	4
TCGAGGG	122030	0.0	28.952923	6
CGAGGGC	131755	0.0	26.744354	7
GATCCGG	115390	0.0	25.951609	6
GAGGGCG	143020	0.0	24.601612	8
ATCCGGC	121015	0.0	24.51216	7
AGGGCGC	145215	0.0	24.11976	9
TCCGGCG	133130	0.0	22.256744	8
CCGGCGC	206300	0.0	14.490372	9
CCGTCGA	135455	0.0	13.088763	16-17
CGCCGTC	139875	0.0	12.828008	14-15
>>END_MODULE