FastQCFastQC Report
Wed 25 May 2016
SRR445724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50896528
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1259200.2474039093590038No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1228580.2413877818934918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA519240.10201874674044564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG453900.064.086612
ATATCTC470550.062.5218541
ATATGGA517550.046.9415741
TATGGAT520050.046.3803562
ATCTCGA630100.045.8930853
TCTCGAG710350.040.4710584
CTCGAGG843150.033.946125
ATGGATC827150.029.408073
GGATCCG883800.026.7790075
TGGATCC910850.026.6494854
TCGAGGG1207300.023.7289946
CGAGGGC1241350.022.9643787
GATCCGG1115000.021.2635566
GAGGGCG1350300.021.10458
ATCCGGC1137650.020.6707027
AGGGCGC1376200.020.6254029
TCCGGCG1237300.019.0172968
GCGCCGT1227250.012.7092879
CCGTCGA1201800.012.00295116-17
CGTCGAC1188500.011.80709812-13
Produced by FastQC (version 0.11.5)