##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445724_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50896528
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432154154012235	33.0	31.0	34.0	29.0	34.0
2	31.700802557691166	34.0	31.0	34.0	29.0	34.0
3	31.805200170039104	34.0	31.0	34.0	30.0	34.0
4	35.18853060075139	37.0	35.0	37.0	32.0	37.0
5	34.94534228346578	37.0	35.0	37.0	32.0	37.0
6	34.88889813859208	37.0	35.0	37.0	32.0	37.0
7	34.84373940006281	37.0	35.0	37.0	32.0	37.0
8	34.804841009980095	37.0	35.0	37.0	32.0	37.0
9	36.401770529416076	39.0	37.0	39.0	32.0	39.0
10-11	36.33904650627642	39.0	37.0	39.0	32.0	39.0
12-13	36.24647527037601	39.0	37.0	39.0	32.0	39.0
14-15	37.47956884210255	40.0	38.0	41.0	32.0	41.0
16-17	37.40903218388492	40.0	38.0	41.0	32.0	41.0
18-19	37.32348370600053	40.0	38.0	41.0	31.5	41.0
20-21	37.235379385800144	40.0	38.0	41.0	31.0	41.0
22-23	37.10482784798209	40.0	37.5	41.0	31.0	41.0
24-25	36.937105159707556	40.0	37.0	41.0	30.5	41.0
26-27	36.69255645493146	40.0	37.0	41.0	30.0	41.0
28-29	36.40721372978526	40.0	36.0	41.0	30.0	41.0
30-31	36.152081375766926	39.5	35.5	41.0	29.5	41.0
32-33	35.920046412596164	39.0	35.0	41.0	29.0	41.0
34-35	35.811893072549076	39.0	35.0	41.0	28.0	41.0
36-37	35.60232025060727	39.0	35.0	41.0	27.0	41.0
38-39	35.3863873288174	39.0	35.0	41.0	25.5	41.0
40-41	35.26360051514712	39.0	35.0	41.0	25.0	41.0
42-43	34.95876161729539	39.0	35.0	41.0	23.5	41.0
44-45	34.59157066666708	39.0	35.0	41.0	20.5	41.0
46-47	34.195142092993066	39.0	34.0	41.0	15.5	41.0
48-49	33.74791193025976	39.0	33.5	41.0	8.0	41.0
50-51	33.39518841049433	39.0	33.0	41.0	2.0	41.0
52-53	33.04521389946285	38.0	33.0	41.0	2.0	41.0
54-55	32.687686446902624	38.0	33.0	40.0	2.0	41.0
56-57	32.26323184559858	37.5	32.0	40.0	2.0	41.0
58-59	31.857329108971832	37.0	31.5	40.0	2.0	41.0
60-61	31.46753014272408	36.5	31.0	40.0	2.0	41.0
62-63	31.03783989941318	36.0	30.5	40.0	2.0	41.0
64-65	30.555825811929648	35.0	30.0	39.0	2.0	41.0
66-67	30.072192891035712	35.0	29.0	39.0	2.0	41.0
68-69	29.58394456690641	35.0	28.5	39.0	2.0	40.5
70-71	29.05255022503696	35.0	27.5	38.0	2.0	40.0
72-73	28.505712138163922	34.5	26.0	37.0	2.0	40.0
74-75	27.932972412184974	34.0	25.5	36.5	2.0	39.0
76-77	27.17219232518179	33.5	24.5	36.0	2.0	39.0
78-79	27.115752699280392	34.0	24.0	35.5	2.0	38.5
80-81	26.733130195049846	34.0	23.5	35.0	2.0	37.0
82-83	26.23056446993791	34.0	20.0	35.0	2.0	37.0
84-85	25.72090633569347	33.5	17.5	35.0	2.0	36.5
86-87	25.243453060295195	33.0	9.5	35.0	2.0	36.0
88-89	24.720317759199606	33.0	2.0	35.0	2.0	36.0
90-91	24.207862538285518	33.0	2.0	35.0	2.0	35.0
92-93	23.574008623928137	32.0	2.0	35.0	2.0	35.0
94-95	22.92008850780548	32.0	2.0	35.0	2.0	35.0
96-97	22.33014701906582	31.0	2.0	35.0	2.0	35.0
98-99	21.626509936001924	31.0	2.0	35.0	2.0	35.0
100	20.522397087675607	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09303821341095286
1101	2	0.0729883658632744
1101	3	0.10467312891370995
1101	4	0.09907109141099824
1101	5	0.15640796716552785
1101	6	0.15637603424571722
1101	7	0.17074565256126562
1101	8	0.15863573435923684
1101	9	0.18731527655479852
1101	10-11	0.17939108673070336
1101	12-13	0.20123947810186849
1101	14-15	0.2228885373063747
1101	16-17	0.19865717268892524
1101	18-19	0.17931592561485843
1101	20-21	0.1734205969461371
1101	22-23	0.17116207588623666
1101	24-25	0.15442458027705186
1101	26-27	0.2011928110655532
1101	28-29	0.17230779626695636
1101	30-31	0.13245579305654331
1101	32-33	0.07786672097837055
1101	34-35	0.09481309914233549
1101	36-37	0.1007752363300014
1101	38-39	0.06001331729983406
1101	40-41	0.05206200172813169
1101	42-43	0.07044233370525177
1101	44-45	0.036426915162387274
1101	46-47	0.019425877822399684
1101	48-49	-0.001911197456820446
1101	50-51	-6.088383531022146E-4
1101	52-53	-0.02989614139121244
1101	54-55	-0.04030486775557307
1101	56-57	-0.03524679045784751
1101	58-59	-0.04469517217190955
1101	60-61	-0.04821477799264784
1101	62-63	-0.10110558064771524
1101	64-65	-0.16294118323727957
1101	66-67	-0.17192627949939876
1101	68-69	-0.1014736547857602
1101	70-71	-0.14525793669944065
1101	72-73	-0.13755736255866324
1101	74-75	-0.23678525034347686
1101	76-77	-0.11373992297172464
1101	78-79	-0.09341702747239111
1101	80-81	-0.10817045130928093
1101	82-83	-0.19692008517064963
1101	84-85	-0.08951926290803058
1101	86-87	-0.2534337342377313
1101	88-89	-0.28307875056565734
1101	90-91	-0.33432908871824907
1101	92-93	-0.42915857445877137
1101	94-95	-0.22200036458901096
1101	96-97	-0.3630652413123805
1101	98-99	-0.24803215206221196
1101	100	-0.2055493262760244
1102	1	0.10004130470434447
1102	2	0.11024387791157864
1102	3	0.12156831517127742
1102	4	0.1219590874735701
1102	5	0.12665479635320764
1102	6	0.17155330371844002
1102	7	0.1641811773440267
1102	8	0.16467329184941804
1102	9	0.18151341224704254
1102	10-11	0.20301842318471586
1102	12-13	0.18716588188127048
1102	14-15	0.18449020858312082
1102	16-17	0.12506568308679533
1102	18-19	0.16962881719999245
1102	20-21	0.17408244514806626
1102	22-23	0.18952535138606663
1102	24-25	0.21417791391223062
1102	26-27	0.19919407848041004
1102	28-29	0.21135148523651992
1102	30-31	0.20997982991893593
1102	32-33	0.13067014215834405
1102	34-35	0.13073319877410938
1102	36-37	0.1595569082935171
1102	38-39	0.09517723680416879
1102	40-41	0.14178515961461358
1102	42-43	0.19294886840983594
1102	44-45	0.1636584684916187
1102	46-47	0.13846728708551836
1102	48-49	0.13143170970357687
1102	50-51	0.12474899011090201
1102	52-53	0.09440387961915775
1102	54-55	0.03473345487041968
1102	56-57	0.10310823414455683
1102	58-59	0.024219161481209284
1102	60-61	0.03350635769519528
1102	62-63	0.014949037265392207
1102	64-65	-0.003343113705124523
1102	66-67	-0.0446223062983222
1102	68-69	0.012830273561561967
1102	70-71	-0.0023895399978499654
1102	72-73	0.057818132792707644
1102	74-75	-0.12385126935643598
1102	76-77	0.029415803185461442
1102	78-79	0.03321520931398325
1102	80-81	-0.03199064364021709
1102	82-83	-0.12892972077870013
1102	84-85	5.368498116204989E-4
1102	86-87	-0.16016347287402866
1102	88-89	-0.21774498850935942
1102	90-91	-0.2728222124463109
1102	92-93	-0.3064363102889942
1102	94-95	-0.08390334715094738
1102	96-97	-0.2541065121588204
1102	98-99	-0.16531112406496007
1102	100	-0.16291044876075134
1103	1	0.06611809088151688
1103	2	0.07243135900254671
1103	3	0.10398170280107877
1103	4	0.12075080585972131
1103	5	0.13130671624721657
1103	6	0.14124820032920837
1103	7	0.11439660739048207
1103	8	0.13515185827241538
1103	9	0.16328276717104728
1103	10-11	0.11878818136026581
1103	12-13	0.1689317882504895
1103	14-15	0.11919594730694172
1103	16-17	0.11926471515549508
1103	18-19	0.1315866533426444
1103	20-21	0.08277174657756348
1103	22-23	0.08866950987310673
1103	24-25	0.15528113994178483
1103	26-27	1.5690634085530064E-4
1103	28-29	0.09995479238239824
1103	30-31	0.12094370314593306
1103	32-33	0.07807472961831508
1103	34-35	0.09407199457707094
1103	36-37	0.10088296419291964
1103	38-39	0.09042578926720068
1103	40-41	0.020560006357683847
1103	42-43	-0.002564098164675954
1103	44-45	0.025754320948045972
1103	46-47	-0.026510406749210347
1103	48-49	0.06032423077932947
1103	50-51	-0.007497302659928096
1103	52-53	-0.003910445458750189
1103	54-55	0.05448470123386073
1103	56-57	0.06433782131632881
1103	58-59	-0.1287961722167168
1103	60-61	-0.12124981936138823
1103	62-63	-0.12247480966251345
1103	64-65	-0.18592861668039617
1103	66-67	-0.03467699594923346
1103	68-69	-0.010864710252278087
1103	70-71	0.003846758248212012
1103	72-73	0.010226620811874199
1103	74-75	-0.08336414440669415
1103	76-77	-8.638292567226813E-4
1103	78-79	0.0055200266992052605
1103	80-81	-0.009101069837679887
1103	82-83	-0.03254752857936083
1103	84-85	0.041829051271623285
1103	86-87	-0.1389907376850701
1103	88-89	-0.17155879278111996
1103	90-91	-0.24299950745726306
1103	92-93	-0.2513716552704537
1103	94-95	-0.17704851212853967
1103	96-97	-0.2985278356436254
1103	98-99	-0.13335339642853938
1103	100	-0.14003449201386786
1104	1	0.04111969022566342
1104	2	0.02354780466460582
1104	3	0.019365116464292953
1104	4	0.011443140074078428
1104	5	0.029122586023262897
1104	6	0.09191629881859598
1104	7	0.0907021665108303
1104	8	0.08396658043392335
1104	9	0.11897702269061483
1104	10-11	0.11714903983121872
1104	12-13	0.10294148840020512
1104	14-15	0.12123048052889374
1104	16-17	0.10625662304596517
1104	18-19	0.10262903848439464
1104	20-21	0.11251727699646352
1104	22-23	0.038469960821863936
1104	24-25	0.08411609848718626
1104	26-27	0.10824270358082089
1104	28-29	0.07038227288123267
1104	30-31	0.07314288279971493
1104	32-33	0.03732080478814481
1104	34-35	0.09713231152434076
1104	36-37	0.09106424496243193
1104	38-39	0.0957069775628625
1104	40-41	0.03356046467816043
1104	42-43	0.08668204682033576
1104	44-45	0.08447867275891241
1104	46-47	0.07057372577051524
1104	48-49	0.08858361292811878
1104	50-51	0.08426157271727419
1104	52-53	0.09594720529744194
1104	54-55	0.03251052940692034
1104	56-57	0.03282798239737872
1104	58-59	-0.04785878930103493
1104	60-61	-0.05207745764908367
1104	62-63	-0.07576890954063131
1104	64-65	-0.026674950051852164
1104	66-67	-0.057322101209333454
1104	68-69	0.027756735739998817
1104	70-71	-0.02756918147750298
1104	72-73	0.079397031316784
1104	74-75	-0.09583466000513852
1104	76-77	-0.015471783681448414
1104	78-79	0.01664499556853727
1104	80-81	7.573410721910534E-4
1104	82-83	-0.048453825724699584
1104	84-85	0.10021866699423754
1104	86-87	-0.04229633981431746
1104	88-89	-0.09224238148410535
1104	90-91	-0.12040106329836675
1104	92-93	-0.14989235938458734
1104	94-95	-0.05809948156257505
1104	96-97	-0.1572723275449377
1104	98-99	-0.06492215798647294
1104	100	-0.11187640216982686
1105	1	-0.0620057057275325
1105	2	-0.0472465466098555
1105	3	-0.027835159137449494
1105	4	-0.01943606391355246
1105	5	-0.018802470643130675
1105	6	-0.021814863861472134
1105	7	-0.011782761324283797
1105	8	-0.012724710377554516
1105	9	0.025221254255562542
1105	10-11	-0.03415085483641178
1105	12-13	0.011476686943666436
1105	14-15	0.018690713162200723
1105	16-17	0.012028821913524723
1105	18-19	-0.00787204724420576
1105	20-21	0.011681006788435866
1105	22-23	0.017074724067086322
1105	24-25	0.012612061027809318
1105	26-27	0.004437496413345343
1105	28-29	-0.0912208210669263
1105	30-31	-0.023881110314604825
1105	32-33	0.017863472593695917
1105	34-35	0.007155075649151854
1105	36-37	-0.006727023103159979
1105	38-39	-0.017782669055186773
1105	40-41	-0.01028592234135317
1105	42-43	-7.810510073156252E-4
1105	44-45	0.007598919157686623
1105	46-47	-0.06460147564676788
1105	48-49	-0.01468319623785419
1105	50-51	0.014571798345180298
1105	52-53	0.04575192496333358
1105	54-55	-0.05197250890273608
1105	56-57	-0.003256380403684034
1105	58-59	0.040113923575656685
1105	60-61	0.03570885019143333
1105	62-63	-0.014547110512065586
1105	64-65	-0.073307750047789
1105	66-67	-0.08568039519170867
1105	68-69	-0.01094621453125555
1105	70-71	0.016249465982738798
1105	72-73	0.085988095264657
1105	74-75	-0.04144248665032535
1105	76-77	-0.0011115232455054525
1105	78-79	0.037718084537690544
1105	80-81	0.05220342444431836
1105	82-83	0.015936904049386413
1105	84-85	0.09482196252156072
1105	86-87	-0.013732149473906219
1105	88-89	0.004852565981039447
1105	90-91	-0.0786785718229126
1105	92-93	-0.15964017035828704
1105	94-95	-0.09173196023522223
1105	96-97	-0.11433162258938623
1105	98-99	0.03935844114609566
1105	100	-0.02858091637478921
1106	1	-0.1931431425586041
1106	2	-0.1739659455173843
1106	3	-0.1311651026739611
1106	4	-0.12011336984640764
1106	5	-0.1640839734509285
1106	6	-0.1916783184292683
1106	7	-0.1968224044672624
1106	8	-0.3230097033172896
1106	9	-0.21464224560138234
1106	10-11	-0.18999205935293162
1106	12-13	-0.1644021859854945
1106	14-15	-0.18381351256768852
1106	16-17	-0.15919772162351364
1106	18-19	-0.18570204075660968
1106	20-21	-0.32060281718998596
1106	22-23	-0.1826554202065509
1106	24-25	-0.16246043139944533
1106	26-27	-0.18907359730014406
1106	28-29	-0.24596472008979475
1106	30-31	-0.17601520303366414
1106	32-33	-0.18277543115850392
1106	34-35	-0.16652503172557687
1106	36-37	-0.16847421197144286
1106	38-39	-0.21093261953743792
1106	40-41	-0.21446182527449764
1106	42-43	-0.1692807863607797
1106	44-45	-0.1895581526936425
1106	46-47	-0.2277046934274125
1106	48-49	-0.18557793486571228
1106	50-51	-0.22798087859994354
1106	52-53	-0.18683284449421222
1106	54-55	-0.22884183291492377
1106	56-57	-0.20154064970587626
1106	58-59	-0.19800347862956258
1106	60-61	-0.374069787287862
1106	62-63	-0.260181875018187
1106	64-65	-0.33050810179075185
1106	66-67	-0.31381232505992784
1106	68-69	-0.28698880719564457
1106	70-71	-0.18117358622466284
1106	72-73	-0.24499126723012665
1106	74-75	-0.3434141397744668
1106	76-77	-0.30863254086932557
1106	78-79	-0.2850064579447569
1106	80-81	-0.2514211929085093
1106	82-83	-0.26845282288722316
1106	84-85	-0.1965060976688271
1106	86-87	-0.3036960284395924
1106	88-89	-0.19944503948880765
1106	90-91	-0.3174999917436594
1106	92-93	-0.41382872990154596
1106	94-95	-0.37277879145801407
1106	96-97	-0.33931015523581465
1106	98-99	-0.1529360842627021
1106	100	-0.24188900163951743
1107	1	-0.14681073522819688
1107	2	-0.1820617455232707
1107	3	-0.1393603234569447
1107	4	-0.09981729531790506
1107	5	-0.1520203042263688
1107	6	-0.2176908317101649
1107	7	-0.18998771984068696
1107	8	-0.17685203795882387
1107	9	-0.20353308885085397
1107	10-11	-0.13714023097952577
1107	12-13	-0.1490196738744416
1107	14-15	-0.22603590705688248
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2207	52-53	-0.03227273237110495
2207	54-55	-6.039146051364241E-4
2207	56-57	0.012516405877818215
2207	58-59	0.0823322330422549
2207	60-61	0.04664686565295639
2207	62-63	0.16428654669038778
2207	64-65	0.15022664949499998
2207	66-67	0.12766975063281194
2207	68-69	0.1091638015551375
2207	70-71	0.13541857629716603
2207	72-73	0.08834450519408321
2207	74-75	0.1805106834154806
2207	76-77	0.11179271191605267
2207	78-79	0.14834947176476376
2207	80-81	0.08206456200152346
2207	82-83	0.15916363788339183
2207	84-85	0.19461937008943053
2207	86-87	0.21477506724381712
2207	88-89	0.2036633248955404
2207	90-91	0.21975422321779803
2207	92-93	0.3227695440179055
2207	94-95	0.3720723708514164
2207	96-97	0.47514827988435826
2207	98-99	0.4344730662011784
2207	100	0.3446302857947998
2208	1	-0.2843163895282643
2208	2	-0.2737755201076375
2208	3	-0.2704227436083677
2208	4	-0.32577259898724975
2208	5	-0.3348151595639024
2208	6	-0.360773241802427
2208	7	-0.35308132026760575
2208	8	-0.32165813339561566
2208	9	-0.3756091814838882
2208	10-11	-0.377092114543089
2208	12-13	-0.34920278154499584
2208	14-15	-0.3840766573155321
2208	16-17	-0.36665858495665304
2208	18-19	-0.44471264316555903
2208	20-21	-0.4113566944456366
2208	22-23	-0.42752108662831745
2208	24-25	-0.4639886561181257
2208	26-27	-0.4181336333706511
2208	28-29	-0.4124597638240459
2208	30-31	-0.38233716581452626
2208	32-33	-0.31374019797852526
2208	34-35	-0.3875370865831371
2208	36-37	-0.3334271466997265
2208	38-39	-0.28781237241540225
2208	40-41	-0.41660181713201894
2208	42-43	-0.30098814772508575
2208	44-45	-0.3113376466630271
2208	46-47	-0.25872933173630486
2208	48-49	-0.3268713188282959
2208	50-51	-0.2544939779761535
2208	52-53	-0.23501406874547826
2208	54-55	-0.23810311607571322
2208	56-57	-1.4296657795279053
2208	58-59	-0.9470667192838498
2208	60-61	-0.4724753239622288
2208	62-63	-0.11384499417989602
2208	64-65	0.04047551341151845
2208	66-67	-0.035378486067436654
2208	68-69	0.004414344896179756
2208	70-71	0.03703607722948732
2208	72-73	0.05555699375547718
2208	74-75	0.14324931862793022
2208	76-77	0.1003693542847941
2208	78-79	0.19675755890215996
2208	80-81	0.207684573573804
2208	82-83	0.3095446571702709
2208	84-85	0.31577316171238934
2208	86-87	0.4027247018926552
2208	88-89	0.3800077800913044
2208	90-91	0.39295314998953046
2208	92-93	0.5675027759255897
2208	94-95	-0.10220037658715952
2208	96-97	0.5816224777456611
2208	98-99	0.5889640110441867
2208	100	-0.27263256251970347
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	807643.0
3	266450.0
4	168026.0
5	140682.0
6	141813.0
7	158705.0
8	177540.0
9	197215.0
10	216594.0
11	226464.0
12	244107.0
13	298317.0
14	424006.0
15	621485.0
16	718128.0
17	709577.0
18	690206.0
19	562438.0
20	493537.0
21	503439.0
22	534195.0
23	580111.0
24	636959.0
25	710806.0
26	797795.0
27	895382.0
28	1007652.0
29	1142403.0
30	1312905.0
31	1508165.0
32	1767507.0
33	2136355.0
34	2705374.0
35	3631837.0
36	5211008.0
37	7671485.0
38	8589124.0
39	2288815.0
40	2278.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.108871739826867	27.953575960432975	26.976028852171947	22.961523447568215
2	23.555664850785156	26.803688301358257	27.995589459346238	21.64505738851035
3	23.57171836105831	26.84063492190734	26.76770263715636	22.819944079877985
4	24.11032956838454	25.941062629190398	27.15863638687854	22.789971415546518
5	24.793272139052604	26.118112535869948	25.76847922065817	23.32013610441928
6	24.918664710683338	25.884486332635596	25.731835849013486	23.465013107667577
7	24.31984796903327	26.488279269621657	25.464334172295345	23.727538589049733
8	24.488193159812724	25.796850010755605	25.909901750283076	23.805055079148595
9	24.352514512451613	26.010181341639456	25.596308412865813	24.04099573304312
10-11	24.734647584212382	25.784312186335402	25.51926874768648	23.96177148176574
12-13	24.203940014015934	25.90586581829762	25.737411696354584	24.152782471331864
14-15	23.798462669327634	26.053694552523027	26.229878587184974	23.917964190964362
16-17	23.61502715400709	26.119255792615455	26.52762253060385	23.738094522773597
18-19	23.13321730419277	26.163887413005533	27.403922203867136	23.298973078934566
20-21	22.589121018600423	26.19119174686621	28.447933376797792	22.77175385773558
22-23	22.363651474301303	26.11694906007194	29.00614356603459	22.513255899592167
24-25	22.129891654615193	25.866666797660965	29.684506452779175	22.318935094944667
26-27	22.075918917225213	25.942479551703467	30.19463142836229	21.78697010270903
28-29	22.066227732058575	26.086980697552104	30.148040936723003	21.698750633666318
30-31	22.004159969649074	26.16131880186539	30.063819198828185	21.77070202965735
32-33	22.016713660910828	26.286169757419785	29.960719867043334	21.73639671462605
34-35	21.997995344412384	26.432963753205712	29.905995171888605	21.663045730493295
36-37	22.004500910570616	26.540673827559896	29.747709170644587	21.707116091224904
38-39	22.074601263325384	26.703214714278158	29.511849944534934	21.71033407786153
40-41	22.119668068863827	27.02286746136295	29.09508808157564	21.762376388197588
42-43	22.239188748434774	27.348997018872762	28.49509471189469	21.91671952079777
44-45	22.43688214998125	27.754552011815427	27.83782069437068	21.970745143832644
46-47	22.497665037503335	28.148570994699035	27.21595658477895	22.13780738301868
48-49	22.745754199493884	28.426169842552962	26.544760150656384	22.283315807296773
50-51	22.759485657153515	28.791488767518477	26.026780720193877	22.422244855134135
52-53	22.803984068293328	29.00176324141287	25.74931767004945	22.44493502024435
54-55	22.870360835501735	29.12049500914336	25.549070473012282	22.460073682342625
56-57	22.83477991949483	29.353990458669525	25.38974562298443	22.421483998851205
58-59	22.8422974031176	29.52007909253981	25.25904446801432	22.378579036328276
60-61	22.836118899789895	29.70701117104096	25.06955562704787	22.38731430212128
62-63	22.86016251000626	29.878535620309716	24.98392503442235	22.277376835261677
64-65	22.870946573529636	30.080154874187514	24.814537814637312	22.234360737645535
66-67	22.88814532344257	30.2152734058932	24.652995451006714	22.243585819657515
68-69	22.887108887228976	30.404374187048806	24.4757889893903	22.23272793633192
70-71	22.889458060890533	30.606317117590066	24.304604955690753	22.199619865828655
72-73	22.881264524662605	30.767125196813723	24.13227901786045	22.219331260663225
74-75	22.956167611984238	30.889430632887034	24.001689974053562	22.15271178107517
76-77	22.673363967073364	31.2727565560991	23.730510837838164	22.323368638989376
78-79	22.675669160237653	31.45124463780134	23.525834440735874	22.347251761225134
80-81	22.72173075389604	31.60454539573603	23.370720043283306	22.30300380708462
82-83	22.688271544720525	31.79075425131038	23.20109149832629	22.31988270564281
84-85	22.642498129162288	31.97904060492756	22.937152674062723	22.44130859184743
86-87	22.714915408777465	32.102224996748774	22.74617648208597	22.436683112387787
88-89	22.706255325627613	32.21277538762474	22.549156157488763	22.531813129258886
90-91	22.750158541255214	32.31189503603702	22.314596325764597	22.623350096943167
92-93	22.743468208273075	32.46530338788195	22.041219073068316	22.750009330776656
94-95	22.798565920522822	32.535729227012986	21.841162363788317	22.824542488675878
96-97	22.761215023917742	32.639351464968385	21.58505165655521	23.014381854558657
98-99	22.783308709505413	32.72544852719312	21.44475663774853	23.04648612555293
100	23.30492975252491	32.38237343025215	20.679078249369446	23.63361856785349
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6219.0
1	4856.5
2	3239.0
3	2994.0
4	3149.5
5	3589.5
6	4203.0
7	4917.0
8	5855.5
9	7101.5
10	8579.5
11	10699.0
12	13775.5
13	18418.0
14	26998.0
15	36502.0
16	56860.0
17	68242.5
18	69930.5
19	85781.5
20	106283.5
21	132279.5
22	164198.5
23	201815.0
24	245498.0
25	296096.0
26	353986.0
27	417246.5
28	491826.0
29	572445.5
30	645478.0
31	721654.5
32	808288.5
33	903024.5
34	1007183.0
35	1123681.5
36	1251097.0
37	1368999.0
38	1476372.0
39	1561734.0
40	1615347.5
41	1659245.0
42	1714187.0
43	1795719.0
44	1890440.0
45	1933729.5
46	1948449.5
47	1939407.0
48	1872163.5
49	1793005.5
50	1714263.0
51	1654207.5
52	1589118.0
53	1479411.5
54	1355019.0
55	1239607.0
56	1123941.5
57	1019146.0
58	954356.5
59	888209.0
60	778054.5
61	679933.5
62	598685.0
63	514643.5
64	438708.5
65	372033.0
66	319331.0
67	267616.5
68	217915.0
69	181802.0
70	155563.0
71	138546.5
72	128738.0
73	121386.5
74	111620.0
75	92528.5
76	74316.0
77	64642.5
78	57351.5
79	45433.5
80	30315.0
81	18003.5
82	9851.0
83	4999.0
84	2700.0
85	1703.0
86	1137.5
87	746.0
88	484.0
89	291.0
90	175.0
91	106.0
92	68.0
93	48.5
94	35.5
95	27.5
96	16.5
97	8.0
98	2.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02583476813978352
2	0.00944661686942575
3	1.9647705635244903E-6
4	0.018519927331781844
5	0.024286528935726227
6	0.00746023382970249
7	0.005277373733626781
8	0.004188890841434213
9	0.005198782911085801
10-11	0.0054915337250509505
12-13	0.007347259522299832
14-15	0.008426900946956539
16-17	0.007212672738698404
18-19	0.006441500292515041
20-21	0.004060198369523359
22-23	0.010451597012668526
24-25	0.0073246646608193
26-27	0.001301660498334975
28-29	0.002451051277996802
30-31	0.007562401899005764
32-33	0.004621140365409601
34-35	0.00981009942367778
36-37	0.0072991226434934815
38-39	0.0036947510447078043
40-41	0.004157454512417821
42-43	0.004960063287617576
44-45	0.012666875823042389
46-47	0.011767010904948173
48-49	0.014694519044599664
50-51	0.011861319891997348
52-53	0.00820782902912356
54-55	0.012410473264502443
56-57	0.02009665570901025
58-59	0.02523060119149974
60-61	0.03784934013573578
62-63	0.028715121785910427
64-65	0.004039568278606353
66-67	0.0014961727841238995
68-69	0.0018567081825306433
70-71	0.004183978915025402
72-73	0.004466905876172929
74-75	0.004431540006029488
76-77	0.003912840577259023
78-79	0.001527609113140291
80-81	4.5877392658296844E-4
82-83	0.001935299005071623
84-85	0.008290349392791586
86-87	0.008176392700107166
88-89	0.005655592067105245
90-91	9.224597795747482E-4
92-93	0.0061349960846052215
94-95	0.008859150470931927
96-97	0.02260468533334926
98-99	0.014682730421218516
100	0.016853801893913077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.0896528E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.826708630978665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84371865517616	32.90963548966129
2	11.401057897402685	9.76539569313966
3	4.061795973821884	5.218600580680562
4	2.056252259545327	3.52250065565357
5	1.1993217535640759	2.568150164734154
6	0.8224783231247793	2.1134423699856506
7	0.5846122214796676	1.7525912089993225
8	0.41759558459003127	1.4307395541456378
9	0.34999206342379763	1.349010731106539
>10	1.9375555078902083	15.37339750525163
>50	0.2116622077941668	6.31170034868394
>100	0.10033413051397297	7.372863294815916
>500	0.004499737122550723	1.3315732727395497
>1k	0.008916970554103626	7.338261929727266
>5k	8.727937066205856E-5	0.24103074613174752
>10k+	1.1943462569146376E-4	1.4011064545436205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	125920	0.2474039093590038	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	122858	0.2413877818934918	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51924	0.10201874674044564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.9647705635244903E-6	0.0	5.894311690573471E-6	1.9647705635244903E-6
2	0.0	3.9295411270489806E-6	0.0	5.894311690573471E-6	1.9647705635244903E-6
3	1.9647705635244903E-6	3.9295411270489806E-6	0.0	1.1788623381146943E-5	7.859082254097961E-6
4	3.9295411270489806E-6	1.1788623381146943E-5	0.0	1.1788623381146943E-5	1.1788623381146943E-5
5	5.894311690573471E-6	1.3753393944671432E-5	0.0	1.5718164508195922E-5	1.3753393944671432E-5
6	5.894311690573471E-6	1.9647705635244904E-5	0.0	1.9647705635244904E-5	1.5718164508195922E-5
7	5.894311690573471E-6	2.5542017325818376E-5	0.0	2.5542017325818376E-5	1.7682935071720413E-5
8	7.859082254097961E-6	2.7506787889342863E-5	0.0	2.7506787889342863E-5	1.9647705635244904E-5
9	1.3753393944671432E-5	2.7506787889342863E-5	0.0	3.1436329016391844E-5	2.1612476198769394E-5
10-11	2.8489173171105112E-5	4.322495239753879E-5	0.0	4.12601818340143E-5	2.3577246762293885E-5
12-13	7.662605197745513E-5	4.813687880635001E-5	0.0	4.813687880635001E-5	2.7506787889342863E-5
14-15	1.7781173599896636E-4	5.5013575778685726E-5	0.0	5.697834634221022E-5	3.8313025988727565E-5
16-17	2.9864512565572253E-4	6.09078874692592E-5	0.0	6.97493550051194E-5	4.322495239753879E-5
18-19	5.196818140522277E-4	7.17141255686439E-5	0.0	8.25203636680286E-5	4.617210824282553E-5
20-21	8.998649180942165E-4	7.85908225409796E-5	0.0	9.725614289446227E-5	5.5013575778685726E-5
22-23	0.0016317419530070892	9.037944592212656E-5	0.0	1.0609761043032248E-4	5.796073162397246E-5
24-25	0.002643598793222202	9.332660176741329E-5	0.0	1.1690384852970718E-4	6.189027275102144E-5
26-27	0.0038961400274690645	9.430898704917554E-5	0.0	1.2083338965675615E-4	6.97493550051194E-5
28-29	0.0068845560545898144	9.823852817622452E-5	0.0	1.3163962775614087E-4	7.466128141393063E-5
30-31	0.009111623488344824	1.0806238099384697E-4	0.0	1.4637540698257453E-4	8.153797838626635E-5
32-33	0.009547802553447261	1.0806238099384697E-4	0.0	1.5619925980019697E-4	8.644990479507758E-5
34-35	0.010113656475742314	1.110095368391337E-4	9.823852817622451E-7	1.6012880092724596E-4	9.627375761270002E-5
36-37	0.011001732770455384	1.1985100437499391E-4	1.9647705635244903E-6	1.6700549789958166E-4	1.110095368391337E-4
38-39	0.012398684641121295	1.2574531606556738E-4	1.9647705635244903E-6	1.7682935071720412E-4	1.1690384852970718E-4
40-41	0.014391944377816893	1.306572424743786E-4	1.9647705635244903E-6	1.7977650656249088E-4	1.2378054550204288E-4
42-43	0.01722612591570097	1.473577922643368E-4	1.9647705635244903E-6	1.8370604768953982E-4	1.2476293078380512E-4
44-45	0.02115075511634114	1.5325210395491023E-4	1.9647705635244903E-6	1.8567081825306435E-4	1.306572424743786E-4
46-47	0.026110818403958715	1.581640303637215E-4	1.9647705635244903E-6	1.8763558881658882E-4	1.3655155416495207E-4
48-49	0.03273013043247272	1.5914641564548373E-4	1.9647705635244903E-6	1.9549467107068678E-4	1.4441063641905004E-4
50-51	0.04162661154411161	1.611111862090082E-4	1.9647705635244903E-6	2.063009091700715E-4	1.52269718673148E-4
52-53	0.05320303970439791	1.6602311261781943E-4	1.9647705635244903E-6	2.1023045029712047E-4	1.5718164508195923E-4
54-55	0.06792408315160516	1.6798788318134392E-4	1.9647705635244903E-6	2.171071472694562E-4	1.6209357149077043E-4
56-57	0.08678096863503144	1.6897026846310616E-4	1.9647705635244903E-6	2.239838442417919E-4	1.6602311261781943E-4
58-59	0.11101936069195133	1.7682935071720412E-4	1.9647705635244903E-6	2.2987815593236536E-4	1.7093503902663066E-4
60-61	0.14112554003683708	1.8665320353482658E-4	1.9647705635244903E-6	2.3184292649588986E-4	1.7289980959015515E-4
62-63	0.17864774587374604	1.9254751522540005E-4	1.9647705635244903E-6	2.347900823411766E-4	1.7486458015367965E-4
64-65	0.22470000311219657	1.9844182691597351E-4	1.9647705635244903E-6	2.3675485290470107E-4	1.8174127712601535E-4
66-67	0.2807588368306773	2.0335375332478474E-4	1.9647705635244903E-6	2.416667793135123E-4	1.8567081825306435E-4
68-69	0.3494187265583224	2.0531852388830924E-4	1.9647705635244903E-6	2.455963204405613E-4	1.9254751522540005E-4
70-71	0.4315903434513254	2.0826567973359597E-4	1.9647705635244903E-6	2.5443778797642146E-4	1.954946710706868E-4
72-73	0.5290301923934773	2.1514237670593167E-4	1.9647705635244903E-6	2.56402558539946E-4	1.9942421219773575E-4
74-75	0.6448799415158535	2.1808953255121843E-4	1.9647705635244903E-6	2.652440260758062E-4	2.0138898276126027E-4
76-77	0.7789804444028088	2.2300145896002966E-4	1.9647705635244903E-6	2.672087966393307E-4	2.063009091700715E-4
78-79	0.9339507402155212	2.2987815593236536E-4	1.9647705635244903E-6	2.6917356720285515E-4	2.1219522086064497E-4
80-81	1.110104209858873	2.3577246762293883E-4	3.9295411270489806E-6	2.7310310832990415E-4	2.1808953255121843E-4
82-83	1.3082906166015882	2.387196234682256E-4	3.9295411270489806E-6	2.789974200204776E-4	2.1907191783298067E-4
84-85	1.5300464110243435	2.3970200874998783E-4	3.9295411270489806E-6	2.8194457586576437E-4	2.239838442417919E-4
86-87	1.7768716954523893	2.416667793135123E-4	3.9295411270489806E-6	2.8882127283810007E-4	2.259486148053164E-4
88	1.9777262606203707	2.495258615676103E-4	3.9295411270489806E-6	2.9275081396514907E-4	2.259486148053164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	45390	0.0	64.08661	2
ATATCTC	47055	0.0	62.521854	1
ATATGGA	51755	0.0	46.941574	1
TATGGAT	52005	0.0	46.380356	2
ATCTCGA	63010	0.0	45.893085	3
TCTCGAG	71035	0.0	40.471058	4
CTCGAGG	84315	0.0	33.94612	5
ATGGATC	82715	0.0	29.40807	3
GGATCCG	88380	0.0	26.779007	5
TGGATCC	91085	0.0	26.649485	4
TCGAGGG	120730	0.0	23.728994	6
CGAGGGC	124135	0.0	22.964378	7
GATCCGG	111500	0.0	21.263556	6
GAGGGCG	135030	0.0	21.1045	8
ATCCGGC	113765	0.0	20.670702	7
AGGGCGC	137620	0.0	20.625402	9
TCCGGCG	123730	0.0	19.017296	8
GCGCCGT	122725	0.0	12.709287	9
CCGTCGA	120180	0.0	12.002951	16-17
CGTCGAC	118850	0.0	11.807098	12-13
>>END_MODULE