FastQCFastQC Report
Wed 25 May 2016
SRR445723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41057289
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT2637500.642395068997371No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2211210.5385669764995931No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT820890.19993770168312863No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT655650.1596914983841237No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448840.10932041811138578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG522050.073.796942
ATATCTC545450.071.246721
ATATGGA738100.059.4927371
TATGGAT741500.058.7204062
ATCTCGA761700.050.3907973
GGATCCG898800.047.6432845
ATGGATC963000.045.3142363
TCTCGAG841900.045.1946684
TGGATCC1065700.040.7632264
CTCGAGG951700.039.887475
GATCCGG1163450.036.7326056
ATCCGGC1321100.031.8795667
TCGAGGG1205400.031.4650636
CGAGGGC1315300.028.7359317
TCCGGCG1492200.028.0950098
GAGGGCG1498800.025.1299448
AGGGCGC1507850.024.8731259
CCGGCGC2028100.020.2981119
CCGTCGA1761850.013.55059116-17
GTCGACT1782950.013.361793518-19
Produced by FastQC (version 0.11.5)