##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445723_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41057289
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.555249495406283	33.0	31.0	34.0	30.0	34.0
2	31.822057369642696	34.0	31.0	34.0	30.0	34.0
3	31.90164089986555	34.0	31.0	34.0	30.0	34.0
4	35.283939010196214	37.0	35.0	37.0	33.0	37.0
5	35.067910718605894	37.0	35.0	37.0	32.0	37.0
6	34.996989182602874	37.0	35.0	37.0	32.0	37.0
7	34.96158414161247	37.0	35.0	37.0	32.0	37.0
8	34.910741476379506	37.0	35.0	37.0	32.0	37.0
9	36.54077106747111	39.0	37.0	39.0	32.0	39.0
10-11	36.45261393171867	39.0	37.0	39.0	32.0	39.0
12-13	36.3959478790721	39.0	37.0	39.0	32.0	39.0
14-15	37.66904095884168	40.0	38.0	41.0	32.0	41.0
16-17	37.62677435911563	40.0	38.0	41.0	32.0	41.0
18-19	37.606134041144315	40.0	38.0	41.0	32.0	41.0
20-21	37.52377314780818	40.0	38.0	41.0	32.0	41.0
22-23	37.43029531248398	40.0	38.0	41.0	32.0	41.0
24-25	37.342350830811064	40.0	38.0	41.0	32.0	41.0
26-27	37.07336037213758	40.0	37.0	41.0	31.0	41.0
28-29	36.81568110841415	40.0	36.0	41.0	31.0	41.0
30-31	36.530936346040775	40.0	35.5	41.0	30.0	41.0
32-33	36.22019688148431	40.0	35.0	41.0	30.0	41.0
34-35	35.969587653485846	40.0	35.0	41.0	29.5	41.0
36-37	35.64790287298317	39.0	35.0	41.0	27.5	41.0
38-39	35.26131994491892	39.0	35.0	41.0	25.5	41.0
40-41	34.83229409033801	39.0	35.0	41.0	23.5	41.0
42-43	34.56456501548361	39.0	35.0	41.0	20.5	41.0
44-45	34.45095302079005	39.0	35.0	41.0	16.5	41.0
46-47	34.19913034199603	39.0	35.0	41.0	11.5	41.0
48-49	33.84390581657742	39.0	34.0	41.0	4.5	41.0
50-51	33.53324884650811	39.0	34.0	41.0	2.0	41.0
52-53	33.25590458493253	39.0	33.0	41.0	2.0	41.0
54-55	32.96916310036934	38.5	33.0	41.0	2.0	41.0
56-57	32.66579438549876	38.0	33.0	41.0	2.0	41.0
58-59	32.35984836943325	37.5	32.5	40.5	2.0	41.0
60-61	32.04732357024352	37.0	32.0	40.0	2.0	41.0
62-63	31.67257560527194	36.5	31.5	40.0	2.0	41.0
64-65	31.284514230834873	36.0	31.0	40.0	2.0	41.0
66-67	30.841909788539617	35.0	31.0	39.0	2.0	41.0
68-69	30.423460411621427	35.0	30.0	39.0	2.0	41.0
70-71	29.968062187447398	35.0	29.5	38.5	2.0	40.5
72-73	29.477675425671677	35.0	29.0	37.5	2.0	40.0
74-75	28.99829204504954	35.0	29.0	37.0	2.0	39.5
76-77	28.19213526251088	34.0	27.0	36.0	2.0	39.0
78-79	28.131668496183465	34.5	27.0	36.0	2.0	39.0
80-81	27.75010577293596	34.0	26.5	35.5	2.0	37.5
82-83	27.318548394658986	34.0	25.5	35.0	2.0	37.0
84-85	26.860229872946555	34.0	24.5	35.0	2.0	36.5
86-87	26.403031408138027	34.0	23.0	35.0	2.0	36.0
88-89	26.01683850826098	34.0	20.0	35.0	2.0	36.0
90-91	25.58692773163859	34.0	16.5	35.0	2.0	35.5
92-93	25.13326744491094	33.5	4.5	35.0	2.0	35.0
94-95	24.674950969120246	33.0	2.0	35.0	2.0	35.0
96-97	24.17572631256779	33.0	2.0	35.0	2.0	35.0
98-99	23.661731866904315	33.0	2.0	35.0	2.0	35.0
100	22.806934549429215	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08680864537317845
1101	2	-0.10629388330897527
1101	3	-0.10533668942184349
1101	4	-0.05589295260728022
1101	5	-0.03908775838824852
1101	6	-0.061158753509630515
1101	7	-0.05756301186283963
1101	8	-0.05827189992194093
1101	9	-0.04892236076782552
1101	10-11	-0.09944899830336595
1101	12-13	-0.11379970310797205
1101	14-15	-0.12248822341619814
1101	16-17	-0.13416452073742136
1101	18-19	-0.14242780718227976
1101	20-21	-0.19510465716980008
1101	22-23	-0.1496746655343557
1101	24-25	-0.1993014293573765
1101	26-27	-0.18142202172517585
1101	28-29	-0.2155003038991623
1101	30-31	-0.2634054518346858
1101	32-33	-0.24937834041988793
1101	34-35	-0.2802521077225961
1101	36-37	-0.29584082825393665
1101	38-39	-0.37560631106420317
1101	40-41	-0.312434284441764
1101	42-43	-0.33311886467582497
1101	44-45	-0.2885999726626878
1101	46-47	-0.3078829086392716
1101	48-49	-0.2957165894624083
1101	50-51	-0.3314188128683071
1101	52-53	-0.3429896815611784
1101	54-55	-0.34232638754777867
1101	56-57	-0.4049752812767551
1101	58-59	-0.4021690180422084
1101	60-61	-0.37348095675222126
1101	62-63	-0.3641536596552015
1101	64-65	-0.38864476242803647
1101	66-67	-0.4794889590181377
1101	68-69	-0.531720009318871
1101	70-71	-0.49947935659133336
1101	72-73	-0.4902725978462996
1101	74-75	-0.48178683310538517
1101	76-77	-0.4838426754941345
1101	78-79	-0.41375692752200877
1101	80-81	-0.4705772398053796
1101	82-83	-0.4140219424789713
1101	84-85	-0.42796127634592196
1101	86-87	-0.5064315320383983
1101	88-89	-0.49084199448095234
1101	90-91	-0.4776603761888367
1101	92-93	-0.5893868358419461
1101	94-95	-0.6744931951214532
1101	96-97	-0.7549543842448507
1101	98-99	-0.8189455375086823
1101	100	-0.7930987232592202
1102	1	-0.04142483295603938
1102	2	-0.03523432611355659
1102	3	-0.03211457600089318
1102	4	-0.0031269258778507947
1102	5	0.008930939737282984
1102	6	0.020552536059902593
1102	7	-0.009249098956892965
1102	8	-0.004409020728793678
1102	9	-0.0362995670878874
1102	10-11	-0.03285663657774762
1102	12-13	-0.021639724024311136
1102	14-15	-0.07722832783974098
1102	16-17	-0.04027260854768855
1102	18-19	-0.05247166261441549
1102	20-21	-0.11026239589992315
1102	22-23	-0.09830101939385827
1102	24-25	-0.11245729208307864
1102	26-27	-0.08406156115360375
1102	28-29	-0.12620726843416463
1102	30-31	-0.10423435915136281
1102	32-33	-0.16563597165596633
1102	34-35	-0.16703888679852952
1102	36-37	-0.2031095096640314
1102	38-39	-0.21966027800146293
1102	40-41	-0.1812325357440585
1102	42-43	-0.1856535746768273
1102	44-45	-0.1968563732698385
1102	46-47	-0.27436134738641016
1102	48-49	-0.2457840917100853
1102	50-51	-0.328734942372904
1102	52-53	-0.2511377255127769
1102	54-55	-0.2530329671711087
1102	56-57	-0.31242355884111106
1102	58-59	-0.35064228300635847
1102	60-61	-0.33328262253134255
1102	62-63	-0.3124679487962574
1102	64-65	-0.27974776114532673
1102	66-67	-0.3068005616221292
1102	68-69	-0.35745475188495135
1102	70-71	-0.3211320155951789
1102	72-73	-0.3384450624504325
1102	74-75	-0.38977806196288256
1102	76-77	-0.3910985791206265
1102	78-79	-0.36628877149891537
1102	80-81	-0.3479387975397401
1102	82-83	-0.2878986670503174
1102	84-85	-0.37781911934034085
1102	86-87	-0.4176437000359776
1102	88-89	-0.42014556827997396
1102	90-91	-0.344674024126018
1102	92-93	-0.39659324415147523
1102	94-95	-0.4613043778627386
1102	96-97	-0.5522861315422176
1102	98-99	-0.5722780268832821
1102	100	-0.5782231441268593
1103	1	9.383899282653374E-4
1103	2	0.041030139339103044
1103	3	-0.017908242081034587
1103	4	0.06609698621763727
1103	5	0.039808481994349165
1103	6	0.039891892501195514
1103	7	0.057927697528484146
1103	8	0.0806372977195835
1103	9	0.10466202406626479
1103	10-11	0.12678267755186567
1103	12-13	0.1166400011687756
1103	14-15	0.100725566471084
1103	16-17	0.13727435902882945
1103	18-19	0.11975631399469222
1103	20-21	0.12910340433568024
1103	22-23	0.14230849722721217
1103	24-25	0.08680350044840424
1103	26-27	0.07371865498681274
1103	28-29	0.05780486646985139
1103	30-31	0.08712047684858248
1103	32-33	0.06104380207040805
1103	34-35	0.09279310843513144
1103	36-37	0.11902581765168918
1103	38-39	0.11962193727520543
1103	40-41	0.1225653728395173
1103	42-43	0.1236707991380186
1103	44-45	0.11272390217469308
1103	46-47	0.07577499230042406
1103	48-49	0.08348848083892335
1103	50-51	0.05232720101752619
1103	52-53	0.06903274737848619
1103	54-55	0.08407918029761419
1103	56-57	0.07048219066575001
1103	58-59	0.08142211493987617
1103	60-61	0.1442603696744129
1103	62-63	0.1043633525339871
1103	64-65	0.12842742954794062
1103	66-67	0.07410266800741994
1103	68-69	0.02076970256677768
1103	70-71	0.0346141672129221
1103	72-73	0.07416987784144169
1103	74-75	0.06968648380501818
1103	76-77	0.04462963299796385
1103	78-79	0.05516444613149929
1103	80-81	0.06022186299319188
1103	82-83	0.07458039307017472
1103	84-85	0.07910185560460192
1103	86-87	-0.02185010013564792
1103	88-89	-0.021421745147261362
1103	90-91	0.0258111510399317
1103	92-93	0.03332366810149168
1103	94-95	-0.0043166932459755
1103	96-97	-0.07424645788239914
1103	98-99	-0.10137705591863622
1103	100	-0.11964164821244694
1104	1	0.050295058284373084
1104	2	0.028116289148982787
1104	3	0.01598245113803287
1104	4	0.04197530599799393
1104	5	0.03295068509718391
1104	6	0.037218842428750065
1104	7	0.04134036515445416
1104	8	0.057119451489370476
1104	9	0.04635810235990334
1104	10-11	0.07939354198980908
1104	12-13	0.04463009044705046
1104	14-15	0.06989735454611434
1104	16-17	0.06398694470416189
1104	18-19	0.06842533048324384
1104	20-21	0.12363926999135089
1104	22-23	0.15296927928815052
1104	24-25	0.08930771688127948
1104	26-27	0.05103289010102685
1104	28-29	0.05017884488724178
1104	30-31	0.06651350261738287
1104	32-33	0.04236812416240099
1104	34-35	0.03547436183330177
1104	36-37	0.04246857671144966
1104	38-39	0.044246544430279755
1104	40-41	-0.014069697117932378
1104	42-43	0.011253495172340422
1104	44-45	0.029748574622189494
1104	46-47	-0.02557422486553662
1104	48-49	0.0399319782855585
1104	50-51	0.05487836512648414
1104	52-53	0.05609205292932273
1104	54-55	0.1014738796984247
1104	56-57	0.07254269614752218
1104	58-59	0.08549382899538216
1104	60-61	0.058401063102536455
1104	62-63	0.018721520996059837
1104	64-65	0.08082084546309432
1104	66-67	0.018778638451429686
1104	68-69	0.006936554195377198
1104	70-71	0.05153219666441444
1104	72-73	0.05434881961239668
1104	74-75	0.02855483410641213
1104	76-77	-0.02944626221879787
1104	78-79	-0.009822496638818734
1104	80-81	-0.029606687040569568
1104	82-83	0.024252789984004153
1104	84-85	-0.07962361393623141
1104	86-87	-0.06315481263937883
1104	88-89	-0.05629062354513792
1104	90-91	-0.15488963697831792
1104	92-93	-0.12794601347744106
1104	94-95	-0.10202236884269666
1104	96-97	-0.1469614921983151
1104	98-99	-0.14823158323148533
1104	100	-0.10437025324675986
1105	1	-0.0185152797261523
1105	2	-0.04956363206776615
1105	3	-0.013624678600603346
1105	4	-0.01753676981206098
1105	5	-0.04545450145914032
1105	6	-0.06540736201817055
1105	7	-0.06350556559816312
1105	8	-0.027550562985545923
1105	9	-0.04065463259976099
1105	10-11	0.007636254858361724
1105	12-13	0.01102692206222855
1105	14-15	-0.022691906435383657
1105	16-17	0.025393520263428115
1105	18-19	0.011682092800050725
1105	20-21	0.021737134849189488
1105	22-23	0.007622065292395064
1105	24-25	-0.021975000082406382
1105	26-27	-0.022311709490395515
1105	28-29	-0.030946856756692398
1105	30-31	-0.11372160076513183
1105	32-33	-0.11113270561918398
1105	34-35	-0.06373164109414375
1105	36-37	-0.04106256083613147
1105	38-39	-0.0638585121861226
1105	40-41	-0.053410938130632246
1105	42-43	-0.043895984034421076
1105	44-45	-0.010422128037447465
1105	46-47	-0.004183249837936387
1105	48-49	-0.039630346617862244
1105	50-51	-0.03602708995326509
1105	52-53	-0.0616588783240033
1105	54-55	-0.051674606149113345
1105	56-57	-0.1269752657210219
1105	58-59	-0.17037172995796368
1105	60-61	-0.12118169604624995
1105	62-63	-0.10543372252782746
1105	64-65	-0.16983757599516025
1105	66-67	-0.10481778357355154
1105	68-69	-0.134406864805662
1105	70-71	-0.11140217152362197
1105	72-73	-0.08397650954287883
1105	74-75	-0.10067882362882585
1105	76-77	-0.10711443629811868
1105	78-79	-0.0726404639767253
1105	80-81	-0.13472888669026517
1105	82-83	-0.07671068234233402
1105	84-85	-0.07890681783072395
1105	86-87	-0.06335996785354325
1105	88-89	-0.11282998065087568
1105	90-91	-0.1041045568701584
1105	92-93	-0.09357120102904659
1105	94-95	-0.11911260647810451
1105	96-97	-0.09545335872089922
1105	98-99	-0.0940061429218062
1105	100	-0.07589295292460108
1106	1	-0.08768515069690253
1106	2	-0.11577503666124045
1106	3	-0.09672604613736979
1106	4	-0.07837218976789728
1106	5	-0.0596785582556123
1106	6	-0.1018833928514411
1106	7	-0.16840791676739286
1106	8	-0.0983258923168222
1106	9	-0.13122212767836317
1106	10-11	-0.12047265637765747
1106	12-13	-0.1501413698992735
1106	14-15	-0.16054825117264215
1106	16-17	-0.11409363372772674
1106	18-19	-0.11142522371253705
1106	20-21	-0.10145375454379746
1106	22-23	-0.04290877479786559
1106	24-25	-0.14871616684358457
1106	26-27	-0.1780935706005522
1106	28-29	-0.12361507520203929
1106	30-31	-0.13857093652190144
1106	32-33	-0.14639263026954552
1106	34-35	-0.18952115117934198
1106	36-37	-0.18833889345156507
1106	38-39	-0.1613864922115198
1106	40-41	-0.1466354612096552
1106	42-43	-0.19296422486089426
1106	44-45	-0.15354134548221055
1106	46-47	-0.14283739646603522
1106	48-49	-0.16870906671802288
1106	50-51	-0.19668836195260297
1106	52-53	-0.1995947794607531
1106	54-55	-0.18112664302202575
1106	56-57	-0.156557680386733
1106	58-59	-0.19440031457504858
1106	60-61	-0.20140916724196956
1106	62-63	-0.16534139710721618
1106	64-65	-0.2184960234535538
1106	66-67	-0.23087064596020568
1106	68-69	-0.2094402368100532
1106	70-71	-0.21995379428948425
1106	72-73	-0.22395168283946276
1106	74-75	-0.19367660544309473
1106	76-77	-0.24529423242966786
1106	78-79	-0.19112468598232368
1106	80-81	-0.23045614026345262
1106	82-83	-0.15053289221312482
1106	84-85	-0.18159722688926294
1106	86-87	-0.1645918872371155
1106	88-89	-0.2347109306542272
1106	90-91	-0.1702702668022269
1106	92-93	-0.18787971249494362
1106	94-95	-0.20414661142589807
1106	96-97	-0.2093505681311747
1106	98-99	-0.22182877752084806
1106	100	-0.1734973904149868
1107	1	-0.20857009366930512
1107	2	-0.2230150572466556
1107	3	-0.19542471854469312
1107	4	-0.21050902846683783
1107	5	-0.19660804558533584
1107	6	-0.22200192829304655
1107	7	-0.21907956872229306
1107	8	-0.26874847484724995
1107	9	-0.2987413900913438
1107	10-11	-0.4245292994964842
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2207	72-73	-0.015787303389998897
2207	74-75	0.0258882894990613
2207	76-77	0.0670151757070272
2207	78-79	0.025149566019891267
2207	80-81	0.009810460463086201
2207	82-83	-0.00650992052427668
2207	84-85	0.04185628864849278
2207	86-87	0.00966354618306653
2207	88-89	0.059902964693012706
2207	90-91	0.06307412007965851
2207	92-93	0.0987133963889697
2207	94-95	0.047320080394328556
2207	96-97	0.04601961939792787
2207	98-99	0.034829188598045846
2207	100	0.07148115004405042
2208	1	-0.2136968737364242
2208	2	-0.2002621377190401
2208	3	-0.20204966839103378
2208	4	-0.24615439273228645
2208	5	-0.2794414630537432
2208	6	-0.2739267160986145
2208	7	-0.2856896996514777
2208	8	-0.3122328129954539
2208	9	-0.38793936465138756
2208	10-11	-0.39783037481641514
2208	12-13	-0.324586595823682
2208	14-15	-0.34410364407494853
2208	16-17	-0.3845838673371773
2208	18-19	-0.41391331247677243
2208	20-21	-0.3628541684335289
2208	22-23	-0.37383362621189775
2208	24-25	-0.3848945976049407
2208	26-27	-0.38176997724384876
2208	28-29	-0.41977991646670887
2208	30-31	-0.3835697129355822
2208	32-33	-0.35020591885738384
2208	34-35	-0.35345082534605865
2208	36-37	-0.3128218045163038
2208	38-39	-0.277134776977924
2208	40-41	-0.28991188786323363
2208	42-43	-0.23586906506912442
2208	44-45	-0.2750204110960155
2208	46-47	-0.24697516395812613
2208	48-49	-0.2475712537077328
2208	50-51	-0.2934852591073991
2208	52-53	-0.23910103134760163
2208	54-55	-0.19033133798983926
2208	56-57	-0.20214981394199327
2208	58-59	-0.13324078985587562
2208	60-61	-0.14245473278986864
2208	62-63	-0.1026106542010865
2208	64-65	-0.026192193420456533
2208	66-67	0.08987605034097967
2208	68-69	0.14388139223557772
2208	70-71	0.10052730143015154
2208	72-73	0.0753318259260638
2208	74-75	0.14163866197077013
2208	76-77	0.14385612263881953
2208	78-79	0.14257737901208856
2208	80-81	0.16729885694545032
2208	82-83	0.19220083137127375
2208	84-85	0.030522186022164277
2208	86-87	0.2229640084680362
2208	88-89	0.3497081332775913
2208	90-91	0.428493859021188
2208	92-93	0.46248776229558786
2208	94-95	0.4911496743176329
2208	96-97	0.615263757568556
2208	98-99	0.6941575512959908
2208	100	0.6343487365427158
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	631310.0
3	200894.0
4	133743.0
5	104248.0
6	103745.0
7	123440.0
8	148306.0
9	159336.0
10	161358.0
11	157578.0
12	175480.0
13	249232.0
14	410208.0
15	555853.0
16	629042.0
17	519383.0
18	500459.0
19	400725.0
20	321956.0
21	316364.0
22	334950.0
23	368218.0
24	417685.0
25	477483.0
26	541793.0
27	612136.0
28	691049.0
29	772936.0
30	884315.0
31	1023015.0
32	1213839.0
33	1496195.0
34	1958477.0
35	2696541.0
36	4093613.0
37	6565963.0
38	8189850.0
39	2708528.0
40	8043.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.64942670669323	28.95926669191194	26.04998685904296	22.34131974235187
2	24.25484630408458	27.079702707972466	28.71769764918036	19.947753338762602
3	23.758663497070756	27.39961749442162	26.376008444223604	22.46571056428402
4	24.575154850010346	25.10168081114236	27.640084746289062	22.683079592558236
5	25.77316069158688	25.33788599934616	25.0609891269025	23.827964182164457
6	26.18757622458054	25.02016944722732	25.345891222606344	23.446363105585796
7	24.817841240146933	26.390941510040626	24.833715763935576	23.95750148587686
8	25.01783194050997	25.006951186726816	26.05520354207387	23.920013330689347
9	24.74313098652442	25.57907723035141	25.231440175858438	24.44635160726573
10-11	25.42963380502376	25.032051752865293	25.365656974027562	24.172657468083386
12-13	24.474647107907437	25.17262825966634	25.835719244448125	24.517005387978095
14-15	23.721211354034846	25.276568136079554	27.030523598566873	23.97169691131872
16-17	23.493047256132407	25.281335855528457	27.648665069937866	23.576951818401266
18-19	22.760750695186807	25.33132178509784	29.103582453737687	22.804345065977667
20-21	21.749429095345857	25.329208144423283	30.98058630651672	21.940776453714136
22-23	21.61183266042612	25.262422852047106	31.596820052892756	21.528924434634018
24-25	21.002813207615578	24.6210052015239	32.91843493110231	21.457746659758207
26-27	20.99245270461925	24.6452998694396	33.95898275334043	20.40326467260072
28-29	20.993923431206344	24.720995005054824	33.93721143228904	20.347870131449792
30-31	20.944560509832797	24.742344195943705	33.88590659391486	20.427188700308633
32-33	20.99959948945502	24.824353399085396	33.74984367616942	20.426203435290166
34-35	21.04892248622262	24.89874815995435	33.672744026325354	20.379585327497676
36-37	21.11846959368526	25.00149075040527	33.37188376531399	20.508155890595482
38-39	21.30215088664042	25.21351633165494	32.89153466692989	20.592798114774745
40-41	21.439584905477	25.60594936857327	32.20645534595589	20.748010379993843
42-43	21.61586916074291	26.00649943386017	31.40303419772012	20.9745972076768
44-45	21.808600300298252	26.540072326863246	30.55340329899358	21.09792407384492
46-47	21.94679610415626	27.016706614236348	29.732037589592796	21.304459692014596
48-49	22.166479413258173	27.468285406467363	28.827950358554098	21.537284821720366
50-51	22.21255652923306	28.076941124567128	28.02836218786261	21.682140158337205
52-53	22.31282065177845	28.424341936008325	27.540954678018498	21.72188273419473
54-55	22.377469348689193	28.64842343618867	27.180458150012786	21.793649065109356
56-57	22.33714471259106	28.982864692981458	26.90839447590806	21.77159611851942
58-59	22.372274166084964	29.19302880505386	26.686922830642867	21.747774198218305
60-61	22.374443120487943	29.425002288933815	26.4418011754756	21.758753415102646
62-63	22.395834608244364	29.679164003739068	26.268917807291125	21.65608358072544
64-65	22.432531555281273	29.893033396952763	26.063296759501775	21.611138288264197
66-67	22.438202868181666	30.06642434899688	25.869189379655122	21.626183403166333
68-69	22.44602053955298	30.26296816493997	25.67175584455973	21.61925545094732
70-71	22.434994405439518	30.4669018211786	25.505070899001314	21.59303287438057
72-73	22.413362898557143	30.598147033099977	25.35028570814095	21.63820436020193
74-75	22.50373652130217	30.71981495492409	25.207685967294513	21.56876255647923
76-77	22.346248687961857	31.023991538947048	24.911546687051096	21.71821308604
78-79	22.387016807092643	31.039792223474	24.805650055051167	21.76754091438219
80-81	22.456020240342436	31.11369682555516	24.603182673601268	21.827100260501137
82-83	22.48232178948327	31.154363451009214	24.477266886441775	21.886047873065742
84-85	22.47998813994721	31.20602831294098	24.25832501501353	22.055658532098278
86-87	22.54425328777906	31.284804487417357	24.033163724663765	22.137778500139817
88-89	22.659627626114744	31.283239828582467	23.837709424521645	22.219423120781144
90-91	22.63120441698773	31.359210208833783	23.664009261458887	22.3455761127196
92-93	22.64997030897409	31.51046148175616	23.459667437858403	22.37990077141135
94-95	22.732391571035258	31.560801159231456	23.299754776650342	22.407052493082947
96-97	22.72354626711958	31.71798100848351	23.079345686716007	22.479127037680904
98-99	22.733839002054843	31.87184526668167	22.934856038429228	22.45945969283426
100	23.0240611601854	31.864716835246565	22.251411120863207	22.859810883704824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2451.0
1	1758.5
2	1058.0
3	1036.0
4	1177.0
5	1426.5
6	1674.0
7	2120.0
8	2776.0
9	3622.0
10	4940.0
11	7062.0
12	10713.0
13	18078.0
14	30848.5
15	44254.0
16	111128.5
17	127539.0
18	85913.5
19	96880.0
20	120749.5
21	140947.5
22	157425.5
23	185122.0
24	218141.5
25	257647.0
26	304260.0
27	356984.5
28	418027.0
29	483374.0
30	546207.0
31	613438.0
32	689060.0
33	761356.0
34	833068.0
35	921205.0
36	1021839.5
37	1128153.5
38	1222873.0
39	1290114.0
40	1346330.5
41	1404187.5
42	1489853.0
43	1586717.5
44	1663232.0
45	1689899.5
46	1679651.5
47	1648389.5
48	1581350.0
49	1505094.0
50	1416746.0
51	1329080.5
52	1234306.5
53	1106433.5
54	981777.5
55	883949.0
56	789065.5
57	703969.5
58	653475.5
59	597953.0
60	510159.5
61	442398.5
62	391322.5
63	334948.0
64	284252.0
65	241505.0
66	208103.0
67	173367.5
68	139479.0
69	115390.5
70	98839.0
71	90114.0
72	86249.5
73	82270.5
74	76145.0
75	61728.5
76	47714.0
77	42208.5
78	38593.5
79	30614.0
80	19865.5
81	11543.5
82	6337.5
83	3082.0
84	1590.5
85	957.5
86	650.0
87	429.0
88	264.0
89	168.0
90	100.5
91	66.0
92	44.0
93	32.5
94	24.0
95	16.5
96	9.0
97	9.5
98	8.0
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14495355501918308
2	0.0049540533472631374
3	2.6791832261501724E-4
4	0.0015198275755615525
5	0.017899379571797835
6	0.006378891699352093
7	0.009959254737934596
8	0.007667335269018858
9	0.0037484208954955602
10-11	0.017711836745967324
12-13	0.006678473096457976
14-15	0.003966408985259596
16-17	0.005627502585472704
18-19	0.006518939913446307
20-21	0.010867741413710974
22-23	0.008430902488471658
24-25	0.007946213886649945
26-27	0.01597036764896971
28-29	0.019215832784283446
30-31	0.013341114655670519
32-33	0.011415756164514419
34-35	0.01626264218273155
36-37	0.010081035793668695
38-39	0.012710288786967889
40-41	0.011358519068319391
42-43	0.0071704685616237355
44-45	0.01161547709591834
46-47	0.001864467963289052
48-49	0.0026097680243817364
50-51	0.0034817203834378837
52-53	0.001891259795550554
54-55	0.0013688190664512701
56-57	0.007071825906479115
58-59	0.00439020705921426
60-61	0.0025720158971041657
62-63	0.0012238996101276926
64-65	0.01625168188771548
66-67	0.00710227117041264
68-69	0.004074794124862945
70-71	0.008701256432201357
72-73	6.539642692921104E-4
74-75	1.2056324517675777E-4
76-77	0.0011216035233110495
78-79	0.001182494051178099
80-81	1.9119625750253503E-4
82-83	7.781809461408912E-4
84-85	0.0044389194815079
86-87	0.0015149563333321887
88-89	0.0011264747655404135
90-91	6.296080581452906E-4
92-93	6.588355115214744E-4
94-95	0.0010521883215426134
96-97	4.0796653670923083E-4
98-99	0.006840441900584328
100	0.006985361356907905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.1057289E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.949112405172876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71879417093042	33.37953226841785
2	11.959324191513296	10.272847179824156
3	4.114189081584481	5.301023079633206
4	1.9148119534060248	3.2895789528641606
5	1.0215137528288925	2.193655449683904
6	0.6955957168171012	1.7925131178080687
7	0.46467997904971775	1.3970314856847794
8	0.3420896744013515	1.1753958302810108
9	0.2638254037953172	1.0197960230650682
>10	1.242609243395808	9.65534046441107
>50	0.09984353924853807	2.933272559296054
>100	0.14294012840495549	14.492656458973595
>500	0.010552042904396507	2.9923759767277325
>1k	0.008948274039713182	6.6477608765633285
>5k	9.616821596683038E-5	0.2794296124696534
>10k+	1.8667946424068418E-4	3.177790664296359
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	263750	0.642395068997371	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	221121	0.5385669764995931	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82089	0.19993770168312863	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65565	0.1596914983841237	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44884	0.10932041811138578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.7424844587279E-6	0.0
2	0.0	2.435621114681975E-6	0.0	9.7424844587279E-6	4.87124222936395E-6
3	0.0	4.87124222936395E-6	0.0	1.4613726688091851E-5	4.87124222936395E-6
4	0.0	7.306863344045926E-6	0.0	1.7049347802773827E-5	7.306863344045926E-6
5	2.435621114681975E-6	7.306863344045926E-6	0.0	2.4356211146819754E-5	7.306863344045926E-6
6	2.435621114681975E-6	9.7424844587279E-6	0.0	2.6791832261501728E-5	1.2178105573409877E-5
7	4.87124222936395E-6	9.7424844587279E-6	0.0	2.9227453376183702E-5	1.7049347802773827E-5
8	4.87124222936395E-6	1.2178105573409877E-5	0.0	3.166307449086568E-5	1.94849689174558E-5
9	7.306863344045926E-6	1.4613726688091851E-5	0.0	4.627680117895753E-5	1.94849689174558E-5
10-11	3.166307449086568E-5	1.94849689174558E-5	0.0	5.1148043408321485E-5	1.94849689174558E-5
12-13	1.485728879956005E-4	2.5574021704160743E-5	0.0	5.3583664523003456E-5	2.1920590032137776E-5
14-15	4.481542851014834E-4	2.9227453376183702E-5	0.0	6.332614898173135E-5	2.1920590032137776E-5
16-17	0.0011216035233110495	3.044526393352469E-5	0.0	7.550425455514124E-5	2.1920590032137776E-5
18-19	0.00229313727947308	4.1405558949593574E-5	0.0	8.402892845652815E-5	2.3138400589478765E-5
20-21	0.0039944186280784395	4.384118006427555E-5	0.0	9.7424844587279E-5	2.9227453376183702E-5
22-23	0.006233972243028515	5.236585396566247E-5	0.0	1.0107827625930197E-4	2.9227453376183702E-5
24-25	0.008787720981772567	5.8454906752367405E-5	0.0	1.0716732904600691E-4	3.288088504820666E-5
26-27	0.011686110108244118	6.576177009641332E-5	0.0	1.1203857127537085E-4	3.4098695605547654E-5
28-29	0.015776735770352497	7.550425455514124E-5	0.0	1.2299886629143976E-4	3.6534316720229625E-5
30-31	0.01917442722533385	7.79398756698232E-5	0.0	1.2908791907814468E-4	3.6534316720229625E-5
32-33	0.021002360871902673	7.915768622716419E-5	0.0	1.3274135075016765E-4	4.5058990621616544E-5
34-35	0.022735305294998898	8.281111789918715E-5	0.0	1.388304035368726E-4	4.627680117895753E-5
36-37	0.024585159531599857	8.524673901386913E-5	1.2178105573409876E-6	1.4248383520889553E-4	4.627680117895753E-5
38-39	0.026392390398693885	8.76823601285511E-5	2.435621114681975E-6	1.5100850911028247E-4	5.114804340832148E-5
40-41	0.028057137430579013	9.133579180057407E-5	2.435621114681975E-6	1.546619407823054E-4	5.601928563768543E-5
42-43	0.02986558610823038	9.7424844587279E-5	2.435621114681975E-6	1.6318661468369235E-4	5.601928563768543E-5
44-45	0.03190907222344856	1.0229608681664296E-4	2.435621114681975E-6	1.680578569130563E-4	5.723709619502641E-5
46-47	0.03425944659911666	1.0229608681664296E-4	2.435621114681975E-6	1.7171128858507924E-4	5.96727173097084E-5
48-49	0.03706406431267296	1.0473170793132494E-4	2.435621114681975E-6	1.8145377304380718E-4	6.210833842439037E-5
50-51	0.040481240736571764	1.0594951848866593E-4	2.435621114681975E-6	1.8632501527317112E-4	6.332614898173135E-5
52-53	0.04468025153828349	1.1082076071802987E-4	2.435621114681975E-6	1.96067499731899E-4	6.81973912110953E-5
54-55	0.049686670739512294	1.1325638183271185E-4	2.435621114681975E-6	2.0337436307594494E-4	7.306863344045925E-5
56-57	0.05580251535847874	1.1934543461941678E-4	2.435621114681975E-6	2.1555246864935482E-4	7.672206511248222E-5
58-59	0.06348081092251366	1.2299886629143976E-4	2.435621114681975E-6	2.2042371087871876E-4	7.79398756698232E-5
60-61	0.07301139634426423	1.278701085208037E-4	2.435621114681975E-6	2.240771425507417E-4	7.79398756698232E-5
62-63	0.08457207196510222	1.2908791907814468E-4	3.653431672022963E-6	2.252949531080827E-4	8.037549678450518E-5
64-65	0.0988655144766134	1.3639478242219062E-4	4.87124222936395E-6	2.265127636654237E-4	8.159330734184618E-5
66-67	0.11683796268185169	1.4370164576623653E-4	7.306863344045926E-6	2.3260181645212865E-4	8.281111789918716E-5
68-69	0.13874028555562934	1.4491945632357753E-4	7.306863344045926E-6	2.350374375668106E-4	8.281111789918716E-5
70-71	0.16547244509982137	1.546619407823054E-4	7.306863344045926E-6	2.4843335369756147E-4	8.402892845652815E-5
72-73	0.19781505788168333	1.5953318301166938E-4	7.306863344045926E-6	2.5939364871363035E-4	9.011798124323309E-5
74-75	0.2370614387131113	1.7171128858507924E-4	7.306863344045926E-6	2.679183226150173E-4	9.255360235791506E-5
76-77	0.28350629774898195	1.7901815192912518E-4	7.306863344045926E-6	2.800964281884272E-4	9.377141291525604E-5
78-79	0.33904454821651764	1.8267158360114815E-4	7.306863344045926E-6	2.91056723204496E-4	9.742484458727901E-5
80-81	0.4040951169474438	1.8388939415848912E-4	7.306863344045926E-6	2.98363586548542E-4	9.864265514462E-5
82-83	0.4804737594827559	1.8997844694519406E-4	7.306863344045926E-6	3.0445263933524694E-4	9.986046570196099E-5
84-85	0.5689550033369227	1.9241406805987603E-4	7.306863344045926E-6	3.105416921219518E-4	1.0229608681664296E-4
86-87	0.6716809772803071	2.0337436307594494E-4	7.306863344045926E-6	3.1663074490865677E-4	1.0473170793132494E-4
88	0.7570348836232221	2.1433465809201382E-4	7.306863344045926E-6	3.336800927114306E-4	1.0473170793132494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	52205	0.0	73.79694	2
ATATCTC	54545	0.0	71.24672	1
ATATGGA	73810	0.0	59.492737	1
TATGGAT	74150	0.0	58.720406	2
ATCTCGA	76170	0.0	50.390797	3
GGATCCG	89880	0.0	47.643284	5
ATGGATC	96300	0.0	45.314236	3
TCTCGAG	84190	0.0	45.194668	4
TGGATCC	106570	0.0	40.763226	4
CTCGAGG	95170	0.0	39.88747	5
GATCCGG	116345	0.0	36.732605	6
ATCCGGC	132110	0.0	31.879566	7
TCGAGGG	120540	0.0	31.465063	6
CGAGGGC	131530	0.0	28.735931	7
TCCGGCG	149220	0.0	28.095009	8
GAGGGCG	149880	0.0	25.129944	8
AGGGCGC	150785	0.0	24.873125	9
CCGGCGC	202810	0.0	20.298111	9
CCGTCGA	176185	0.0	13.550591	16-17
GTCGACT	178295	0.0	13.3617935	18-19
>>END_MODULE