FastQCFastQC Report
Wed 25 May 2016
SRR445722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32865315
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT4152191.2633957714995279No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3701911.1263881085576086No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320450.40177615823855634No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840340.25569205711249077No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT557260.16955869736833495No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524980.1597367924208242No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT523820.15938383672878229No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT518990.15791420225243544No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458030.13936577209133702No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451880.13749449837921832No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449300.13670947623657342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403330.12272208557867162No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385130.11718433247939355No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360570.10971140851685128No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT353010.10741111107561269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC705000.079.639111
TATCTCG710150.078.612022
ATATGGA839550.070.322991
TATGGAT871100.067.433192
ATGGATC1015400.057.8013043
ATCTCGA1011850.054.9749763
TGGATCC1114050.052.480734
GGATCCG1121700.051.729185
TCTCGAG1095550.050.4748234
CTCGAGG1228600.044.8367925
GATCCGG1365300.042.4067466
TCGAGGG1394150.039.4389156
ATCCGGC1499350.038.34867
CGAGGGC1561550.035.153567
TCCGGCG1645800.034.8447958
GAGGGCG1728250.031.7165538
AGGGCGC1785150.030.5806149
CCGGCGC2055450.027.757339
CCGTCGA1895250.016.93736616-17
CGCCGTC1917550.016.81229414-15
Produced by FastQC (version 0.11.5)