##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR445722_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32865315 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10373772471068 34.0 31.0 34.0 30.0 34.0 2 32.363045417334355 34.0 31.0 34.0 30.0 34.0 3 32.43304249480037 34.0 31.0 34.0 30.0 34.0 4 35.752460975955955 37.0 37.0 37.0 35.0 37.0 5 35.58264334298941 37.0 37.0 37.0 35.0 37.0 6 35.54622756544399 37.0 36.0 37.0 35.0 37.0 7 35.5091916508331 37.0 36.0 37.0 35.0 37.0 8 35.4674922787139 37.0 36.0 37.0 35.0 37.0 9 37.20403875636062 39.0 38.0 39.0 35.0 39.0 10-11 37.1339729438163 39.0 38.0 39.0 34.5 39.0 12-13 37.08267729976116 39.0 38.0 39.0 34.0 39.0 14-15 38.473302781366925 41.0 39.0 41.0 34.5 41.0 16-17 38.465539764338175 41.0 39.0 41.0 34.5 41.0 18-19 38.42745242210519 41.0 39.0 41.0 34.0 41.0 20-21 38.36111865959599 41.0 39.0 41.0 34.0 41.0 22-23 38.298675259920685 41.0 39.0 41.0 34.0 41.0 24-25 38.214394917559744 41.0 39.0 41.0 34.0 41.0 26-27 37.950075375817946 40.0 38.0 41.0 34.0 41.0 28-29 37.688001621162 40.0 38.0 41.0 33.0 41.0 30-31 37.418449054877456 40.0 37.0 41.0 33.0 41.0 32-33 37.044896146591014 40.0 35.5 41.0 33.0 41.0 34-35 36.79356685003628 40.0 35.0 41.0 32.0 41.0 36-37 36.49635418069171 40.0 35.0 41.0 31.0 41.0 38-39 36.128155564612726 40.0 35.0 41.0 30.0 41.0 40-41 35.73823213013476 39.5 35.0 41.0 28.0 41.0 42-43 35.49188836315733 40.0 35.0 41.0 25.5 41.0 44-45 35.39102327179886 40.0 35.0 41.0 24.5 41.0 46-47 35.10475504342496 40.0 35.0 41.0 22.0 41.0 48-49 34.723507609770365 40.0 35.0 41.0 16.5 41.0 50-51 34.382802857663165 40.0 35.0 41.0 10.5 41.0 52-53 34.14084296773056 39.0 35.0 41.0 7.5 41.0 54-55 33.88903374271629 39.0 34.5 41.0 6.5 41.0 56-57 33.622212962206504 39.0 34.0 41.0 2.0 41.0 58-59 33.33604246604665 38.0 34.0 41.0 2.0 41.0 60-61 33.03485936465237 37.5 33.5 41.0 2.0 41.0 62-63 32.65253806330473 37.0 33.0 40.5 2.0 41.0 64-65 32.28510546757273 36.5 33.0 40.0 2.0 41.0 66-67 31.888711837996986 36.0 33.0 40.0 2.0 41.0 68-69 31.442090681315545 35.0 32.0 39.0 2.0 41.0 70-71 30.918480349267913 35.0 31.5 39.0 2.0 41.0 72-73 30.44495630119474 35.0 31.0 38.5 2.0 40.5 74-75 29.949881797877183 35.0 30.5 37.0 2.0 40.0 76-77 29.132094900048884 34.5 29.5 36.0 2.0 39.0 78-79 28.98000884823407 35.0 29.5 36.0 2.0 39.0 80-81 28.50595089382226 35.0 29.0 36.0 2.0 38.0 82-83 28.010023880799558 35.0 28.5 35.0 2.0 37.0 84-85 27.51153542876434 35.0 26.5 35.0 2.0 37.0 86-87 26.987253431162912 34.0 25.0 35.0 2.0 36.0 88-89 26.516969120788893 34.0 23.5 35.0 2.0 36.0 90-91 26.03564776421586 34.0 19.5 35.0 2.0 36.0 92-93 25.527125679458724 34.0 11.0 35.0 2.0 35.5 94-95 25.0605023867868 34.0 2.0 35.0 2.0 35.0 96-97 24.529501329897492 34.0 2.0 35.0 2.0 35.0 98-99 24.016067486345406 33.0 2.0 35.0 2.0 35.0 100 23.16771289123503 32.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2941191618431098 1101 2 -0.2693950436572834 1101 3 -0.22460233968946852 1101 4 -0.17721488038463917 1101 5 -0.22610862127428533 1101 6 -0.23483062644564967 1101 7 -0.19743276920640085 1101 8 -0.23543189054393565 1101 9 -0.2920604388184884 1101 10-11 -0.25305979876034 1101 12-13 -0.28256408126457444 1101 14-15 -0.3552929027653491 1101 16-17 -0.3608124490864668 1101 18-19 -0.34299706170769895 1101 20-21 -0.34506766692656754 1101 22-23 -0.3025863192902918 1101 24-25 -0.3804368252817909 1101 26-27 -0.33325834604010396 1101 28-29 -0.3351998833975358 1101 30-31 -0.41008780934168243 1101 32-33 -0.40237689089122597 1101 34-35 -0.38181430893955337 1101 36-37 -0.4265251310342535 1101 38-39 -0.47502736099836085 1101 40-41 -0.43814356372678276 1101 42-43 -0.43230116582633116 1101 44-45 -0.4725255532818977 1101 46-47 -0.5118963204403428 1101 48-49 -0.4469293234399885 1101 50-51 -0.4727493443976485 1101 52-53 -0.485981960889319 1101 54-55 -0.45734572612304447 1101 56-57 -0.46552036085405746 1101 58-59 -0.4577040556589722 1101 60-61 -0.4828702859895273 1101 62-63 -0.5511262578157741 1101 64-65 -0.5001140607123133 1101 66-67 -0.5775785557782953 1101 68-69 -0.6291738405521485 1101 70-71 -0.6271286462189387 1101 72-73 -0.6943018498444005 1101 74-75 -0.6240162405176903 1101 76-77 -0.6039431962891406 1101 78-79 -0.6273636329262793 1101 80-81 -0.5337788543164699 1101 82-83 -0.47517687008578946 1101 84-85 -0.5093758808916107 1101 86-87 -0.5717357645711587 1101 88-89 -0.6440679006602927 1101 90-91 -0.6023365567648398 1101 92-93 -0.639380565908926 1101 94-95 -0.6938544994681095 1101 96-97 -0.6966448751936021 1101 98-99 -0.7308959949761942 1101 100 -0.6959156273755305 1102 1 -0.06235356160527061 1102 2 -0.0638428378979583 1102 3 -0.06509411367180462 1102 4 -0.03975887104498099 1102 5 -0.049014301620765366 1102 6 -0.02825700457594138 1102 7 -0.041882205237527614 1102 8 -0.045541067935182866 1102 9 -0.02525789903944542 1102 10-11 -0.04664716749823583 1102 12-13 -0.0543568521883131 1102 14-15 -0.07878843292544246 1102 16-17 -0.07363401016766602 1102 18-19 -0.05949057938477864 1102 20-21 -0.09801283413079886 1102 22-23 -0.11912849847463747 1102 24-25 -0.12625051887771122 1102 26-27 -0.12159731956226238 1102 28-29 -0.11776555955224666 1102 30-31 -0.11204497532219193 1102 32-33 -0.1523769245156288 1102 34-35 -0.18367696841193037 1102 36-37 -0.14393380988206417 1102 38-39 -0.10429449566269966 1102 40-41 -0.1656321515831607 1102 42-43 -0.1742962660980254 1102 44-45 -0.16053105487316088 1102 46-47 -0.20163721193084427 1102 48-49 -0.19619213052891382 1102 50-51 -0.15455932802781547 1102 52-53 -0.11792407857656428 1102 54-55 -0.12237972357899451 1102 56-57 -0.17844333549506075 1102 58-59 -0.11265264312149981 1102 60-61 -0.1608758431824171 1102 62-63 -0.1567116457423836 1102 64-65 -0.15068056166375499 1102 66-67 -0.18368460415654297 1102 68-69 -0.19881994614938137 1102 70-71 -0.1924456959109513 1102 72-73 -0.19270408490511315 1102 74-75 -0.2483145901537256 1102 76-77 -0.20713058081846825 1102 78-79 -0.23071555899290175 1102 80-81 -0.1933511070829148 1102 82-83 -0.14294584281238798 1102 84-85 -0.23238188957954264 1102 86-87 -0.24845386625499444 1102 88-89 -0.22579539775362534 1102 90-91 -0.17566647960623882 1102 92-93 -0.21213170912813695 1102 94-95 -0.23681322989803277 1102 96-97 -0.26462552794489724 1102 98-99 -0.28966163316716376 1102 100 -0.2703208391470433 1103 1 -0.008210231331709394 1103 2 0.010861290869975448 1103 3 0.002134979329646569 1103 4 0.03598602070802315 1103 5 0.043720526980074226 1103 6 0.06903431639207724 1103 7 0.041542081158965516 1103 8 0.03240618167627929 1103 9 0.024034849855603113 1103 10-11 0.045732588727105394 1103 12-13 0.07934660053762599 1103 14-15 0.057444898961911406 1103 16-17 0.03091063893413093 1103 18-19 0.056025317309178035 1103 20-21 0.040817607678476975 1103 22-23 0.04430103728613943 1103 24-25 0.03701347083386963 1103 26-27 0.0369718798582781 1103 28-29 0.05086101252268094 1103 30-31 0.03161304811830945 1103 32-33 0.03780743951198673 1103 34-35 0.036238899311491934 1103 36-37 0.04146489681721022 1103 38-39 0.03495959959982997 1103 40-41 0.06980738235198913 1103 42-43 0.08242983117213498 1103 44-45 0.025445929138193435 1103 46-47 0.01842790943005923 1103 48-49 -0.02307383716772904 1103 50-51 0.036803410042750784 1103 52-53 0.015631408836881633 1103 54-55 -0.019473286267498224 1103 56-57 -0.019611961524368837 1103 58-59 -0.021839293804902127 1103 60-61 -0.04614907907441079 1103 62-63 -0.04610756784590109 1103 64-65 0.024515052715052832 1103 66-67 0.03629238236504051 1103 68-69 0.04436518727459671 1103 70-71 0.08083760355818725 1103 72-73 0.10460268593686806 1103 74-75 0.022215174561523554 1103 76-77 0.04463365669752406 1103 78-79 -0.0097560934536709 1103 80-81 0.020353562106510736 1103 82-83 0.08009486875190674 1103 84-85 0.02821004418796491 1103 86-87 0.04094267820542541 1103 88-89 0.01743224482533634 1103 90-91 0.09283103514614055 1103 92-93 0.03210866345348862 1103 94-95 0.003172999873726212 1103 96-97 -0.02358439404187962 1103 98-99 -0.14778733437027824 1103 100 -0.08305477519136772 1104 1 0.06043330703563754 1104 2 0.044883472634687394 1104 3 0.07167001380128823 1104 4 0.055444447392702045 1104 5 0.06335464028283155 1104 6 0.05334740635446167 1104 7 0.05633160363331058 1104 8 0.08843366232195393 1104 9 0.03904755134166038 1104 10-11 0.04613098911110569 1104 12-13 0.08262696751597076 1104 14-15 0.09463793716388835 1104 16-17 0.11956189041958254 1104 18-19 0.12886647304694776 1104 20-21 0.12239891641809919 1104 22-23 0.11671549285114935 1104 24-25 0.1279018652942554 1104 26-27 0.13743040596003908 1104 28-29 0.13708828320704214 1104 30-31 0.12554892758477365 1104 32-33 0.08215446110165914 1104 34-35 0.07501994835745052 1104 36-37 0.06327964255631713 1104 38-39 0.1085141199755455 1104 40-41 0.058467180066131164 1104 42-43 0.07920676317856135 1104 44-45 0.08966291644887292 1104 46-47 0.00861098996018228 1104 48-49 0.02021764108319246 1104 50-51 0.024398572222970927 1104 52-53 -0.053204320313682274 1104 54-55 -0.0016508606244940438 1104 56-57 0.028211497621413173 1104 58-59 0.07103146294473106 1104 60-61 0.019699083705312148 1104 62-63 -0.030907577383253226 1104 64-65 -0.053910923530096966 1104 66-67 0.001973046713651172 1104 68-69 -0.0635298586286801 1104 70-71 0.033271855441814324 1104 72-73 0.03900919951739468 1104 74-75 0.018525779901338524 1104 76-77 0.05272641427427871 1104 78-79 -0.003522448927764543 1104 80-81 -0.04972473115070031 1104 82-83 0.05792215136641943 1104 84-85 0.0363125217985143 1104 86-87 0.008718038226234626 1104 88-89 -0.05589573757721311 1104 90-91 -0.01805847570959429 1104 92-93 -0.018148354581047244 1104 94-95 -0.005206596871133229 1104 96-97 0.018586875874316178 1104 98-99 -0.08804684528448092 1104 100 -0.059819882803790136 1105 1 0.021112211118918367 1105 2 0.038215173983964235 1105 3 0.06298723226608871 1105 4 0.05782703753833118 1105 5 0.04739019020456681 1105 6 0.04567452411944828 1105 7 0.047968410391156624 1105 8 0.07307964396570554 1105 9 0.05363198081178666 1105 10-11 0.06114006647199233 1105 12-13 0.0887318663723562 1105 14-15 0.0930227523330629 1105 16-17 0.0922554502966193 1105 18-19 0.07152665733577379 1105 20-21 0.0920085400989592 1105 22-23 0.12164019270695547 1105 24-25 0.08520445514958652 1105 26-27 0.09761297753944831 1105 28-29 0.037332288739897024 1105 30-31 0.07261331988033248 1105 32-33 0.07277670649177281 1105 34-35 0.10415723237696284 1105 36-37 0.08875389094341557 1105 38-39 0.10507774005854742 1105 40-41 0.10032249000495597 1105 42-43 0.07378875542283936 1105 44-45 0.12591571411392977 1105 46-47 0.14552430433035113 1105 48-49 0.1264049089334236 1105 50-51 0.12981640232654712 1105 52-53 0.10857297671233823 1105 54-55 0.10881095409320096 1105 56-57 0.12655830547923586 1105 58-59 0.07477115785145116 1105 60-61 0.10698922088348439 1105 62-63 0.11071923421846463 1105 64-65 0.1572675319695307 1105 66-67 0.13629779305963297 1105 68-69 0.146249548257682 1105 70-71 0.03796920935477033 1105 72-73 0.11937245069089997 1105 74-75 0.13393878838757445 1105 76-77 0.14472826796024307 1105 78-79 0.14455648365717977 1105 80-81 0.1081901781943877 1105 82-83 0.16760895766623918 1105 84-85 0.1336050137859104 1105 86-87 0.12003137322142976 1105 88-89 0.07690046782444782 1105 90-91 0.14050510679416917 1105 92-93 0.13714320794896295 1105 94-95 0.11904123553745549 1105 96-97 0.1303853549460925 1105 98-99 0.09129209752040168 1105 100 0.013154299310208728 1106 1 0.03352202170992058 1106 2 0.009672304154477729 1106 3 0.04476990882780996 1106 4 0.06026651041015185 1106 5 0.0645123195507793 1106 6 0.05175028810694471 1106 7 0.07542942576908729 1106 8 0.08745895423570005 1106 9 0.06828187234184213 1106 10-11 0.08455752302711517 1106 12-13 0.10674444371692715 1106 14-15 0.11840626755918748 1106 16-17 0.13661424162988567 1106 18-19 0.13394291187087504 1106 20-21 0.08404169453014276 1106 22-23 0.10249752763167663 1106 24-25 0.10957421104368592 1106 26-27 0.10257063368838715 1106 28-29 0.09062418049319376 1106 30-31 0.07522132556065486 1106 32-33 0.08578614502392412 1106 34-35 0.056106634302516056 1106 36-37 0.03958207336118846 1106 38-39 0.09324900966939254 1106 40-41 0.10739232935269882 1106 42-43 0.06727154798022639 1106 44-45 0.08066287217560131 1106 46-47 0.04477782045069034 1106 48-49 0.08853417113800077 1106 50-51 0.07603585086602038 1106 52-53 0.014364902706830662 1106 54-55 0.08922490055756072 1106 56-57 0.07448217962699744 1106 58-59 0.04571667735380203 1106 60-61 0.03897148931400096 1106 62-63 0.07289462351070597 1106 64-65 0.04840874629520897 1106 66-67 -0.002158883165563452 1106 68-69 0.02999207665096293 1106 70-71 -0.003948040358789484 1106 72-73 0.03028265060491364 1106 74-75 0.0019436678167394916 1106 76-77 0.01500613883080959 1106 78-79 0.022685774606710396 1106 80-81 0.010232804781356464 1106 82-83 0.04282084351581972 1106 84-85 -0.006585525231347589 1106 86-87 0.02830882492982667 1106 88-89 -0.041826089644086295 1106 90-91 0.047418279382341666 1106 92-93 0.04901370601822563 1106 94-95 -0.02589153660709087 1106 96-97 -0.02567131795730404 1106 98-99 -0.04960351467102697 1106 100 -0.10640875587563414 1107 1 -0.008563426284901254 1107 2 0.01396675881736087 1107 3 -0.012606069836074596 1107 4 0.005697115990273005 1107 5 0.013543622285517642 1107 6 -0.01338804373602187 1107 7 -0.00553510434752269 1107 8 0.009593615387409216 1107 9 -0.07928538538093477 1107 10-11 0.034233529926808615 1107 12-13 0.037955451428580034 1107 14-15 0.013383295136129902 1107 16-17 0.022936527539087592 1107 18-19 -0.02386724767769266 1107 20-21 -0.026968738501508938 1107 22-23 0.01136065677525977 1107 24-25 -0.03999258776730841 1107 26-27 -0.013231782233326328 1107 28-29 0.03429802907968593 1107 30-31 0.002551663789347458 1107 32-33 -0.02303606393419244 1107 34-35 -0.06464474259117026 1107 36-37 -0.06764278233276144 1107 38-39 -0.03914111339270221 1107 40-41 -0.10413524600710389 1107 42-43 -0.17127395311302962 1107 44-45 -0.15614308039195635 1107 46-47 -0.13082475959917161 1107 48-49 -0.15190223045073026 1107 50-51 -0.18550150396485776 1107 52-53 -0.15471072626986881 1107 54-55 -0.11292449214621314 1107 56-57 -0.09729320200241887 1107 58-59 -0.09939674116549924 1107 60-61 -0.12912974977916747 1107 62-63 -0.11106471319863687 1107 64-65 -0.1463034058245114 1107 66-67 -0.13438304767770148 1107 68-69 -0.08516105804850937 1107 70-71 -0.08409694255982458 1107 72-73 -0.028922320312204874 1107 74-75 -0.07719204222339471 1107 76-77 -0.061286957758202476 1107 78-79 0.04167813364398043 1107 80-81 -0.09899480616862277 1107 82-83 -0.007458512188044608 1107 84-85 -0.02923528766233119 1107 86-87 -0.059214964279131976 1107 88-89 -0.008990991991453257 1107 90-91 0.0759387492478183 1107 92-93 0.06421033620572913 1107 94-95 0.004208999466293051 1107 96-97 0.05414545074491528 1107 98-99 0.14970463274489276 1107 100 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0.12780846134206314 2207 64-65 0.09715332314702607 2207 66-67 0.1628446225830089 2207 68-69 0.22568715623119218 2207 70-71 0.19286671003500544 2207 72-73 0.20593356093537452 2207 74-75 0.25427082473296636 2207 76-77 0.24405863293401708 2207 78-79 0.18222927540410083 2207 80-81 0.11898105807263093 2207 82-83 0.08652490139238722 2207 84-85 0.11696190276463625 2207 86-87 0.13514348728601888 2207 88-89 0.11939707747922412 2207 90-91 0.045330187690694856 2207 92-93 0.0507055752838923 2207 94-95 0.09620114221423748 2207 96-97 0.1377534274900789 2207 98-99 0.16235303666700318 2207 100 0.15887804246704107 2208 1 -0.07159185988948025 2208 2 -0.10311335558969148 2208 3 -0.11347081210521992 2208 4 -0.058701778589401954 2208 5 -0.06613596928099241 2208 6 -0.09175030676152574 2208 7 -0.08110707623041691 2208 8 -0.10044052503493361 2208 9 -0.11680697999448597 2208 10-11 -0.17505251858306536 2208 12-13 -0.12733621205710932 2208 14-15 -0.13606871099182172 2208 16-17 -0.17762386413814113 2208 18-19 -0.15696043098346735 2208 20-21 -0.09714207360298133 2208 22-23 -0.06252993714853261 2208 24-25 -0.11325492250571756 2208 26-27 -0.548674884290314 2208 28-29 -0.24239325816292734 2208 30-31 -0.185575923184075 2208 32-33 -0.1720606314049391 2208 34-35 -0.1346943191987009 2208 36-37 -0.062239443339798584 2208 38-39 -0.05551329218897649 2208 40-41 -0.040343675719960004 2208 42-43 0.01828305127955332 2208 44-45 -0.062162276020579554 2208 46-47 -0.010598960970739313 2208 48-49 -0.032667734132473925 2208 50-51 0.015130707515936592 2208 52-53 0.03821501147146478 2208 54-55 0.044161931917457764 2208 56-57 0.0935657181562135 2208 58-59 0.1252700147856345 2208 60-61 0.13738242358714814 2208 62-63 0.08187690040153939 2208 64-65 0.12095184711312612 2208 66-67 0.16313255392848447 2208 68-69 0.19584578851285173 2208 70-71 0.18988238752130115 2208 72-73 0.22786075798040528 2208 74-75 0.27119400165751273 2208 76-77 0.29277411522969743 2208 78-79 0.29476241192759645 2208 80-81 0.29477189228786216 2208 82-83 0.30999006313078326 2208 84-85 0.3876696980795131 2208 86-87 0.42828698882890137 2208 88-89 0.4929928860073254 2208 90-91 0.5171455119928439 2208 92-93 0.5817817952308708 2208 94-95 0.6749137333734829 2208 96-97 0.722639843540005 2208 98-99 0.7917696882709144 2208 100 0.7260551933629777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 349789.0 3 119660.0 4 77630.0 5 62474.0 6 63649.0 7 78989.0 8 98289.0 9 102391.0 10 97218.0 11 92052.0 12 98275.0 13 146020.0 14 256662.0 15 358037.0 16 432052.0 17 374305.0 18 390626.0 19 324425.0 20 246903.0 21 240599.0 22 258423.0 23 291285.0 24 337168.0 25 392060.0 26 445797.0 27 507087.0 28 562130.0 29 616074.0 30 686350.0 31 772050.0 32 893988.0 33 1082952.0 34 1474867.0 35 1953063.0 36 3002620.0 37 5318445.0 38 7416379.0 39 2835211.0 40 9321.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.953531912197363 30.596924629103984 25.595715268347096 21.853828190351553 2 23.410500891778295 26.673418653058746 30.243559605516673 19.67252084964629 3 23.17866854265155 28.36497358929532 26.09877249063313 22.35758537741999 4 24.041117173117215 24.65270754054002 29.026057295085977 22.280117991256787 5 25.883785532637216 25.071663139329697 24.563269505688528 24.48128182234456 6 26.724314716198183 24.650787024201303 25.57316738027589 23.05173087932462 7 24.46581645546798 26.94013583437694 24.306073597164453 24.287974112990625 8 25.57176901536583 24.539218907694796 26.194443124113608 23.694568952825765 9 24.620590602826805 25.83710341560888 24.603674643073706 24.93863133849061 10-11 26.29437209019262 24.501122843319234 24.7739996348477 24.430505431640448 12-13 24.846779998056746 24.627721047774163 25.325100395172147 25.200398558996945 14-15 23.906579392590935 24.8195506750342 26.71169734708881 24.562172585286053 16-17 23.49378924228981 24.879175845807115 27.538819555894626 24.088215356008444 18-19 22.69056464808111 25.085745048725975 29.29446888808981 22.9292214151031 20-21 20.99895423154308 25.336310441423414 31.93580644155573 21.72892888547777 22-23 20.730661795951168 25.196304615855404 32.82598826806918 21.24704532012425 24-25 19.72851260030357 24.134458211205143 34.8892197520273 21.247809436463985 26-27 19.711889001332786 24.187030847947856 36.72525531888572 19.37582483183363 28-29 19.715151549272285 24.272425483728675 36.70610912533603 19.306313841663012 30-31 19.686718471290135 24.29671316570408 36.644538149281594 19.372030213724194 32-33 19.697849149173077 24.397521620609723 36.53166738317471 19.372961847042493 34-35 19.746183432402393 24.504943177022525 36.43176494527593 19.317108445299155 36-37 19.789003401919437 24.655275713703222 36.11837949354609 19.43734139083125 38-39 19.915821711054058 24.932364094811437 35.64061510013766 19.511199093996854 40-41 20.02420913383542 25.37372544180422 34.9634717083989 19.63859371596147 42-43 20.175984807376963 25.871624014955287 34.14500310990282 19.807388067764936 44-45 20.313490591451302 26.548726476041836 33.20803724720467 19.929745685302187 46-47 20.47101158231986 27.248729844373077 32.21317498012491 20.06708359318215 48-49 20.678486133257884 28.028897107454938 31.003617691448504 20.288999067838677 50-51 20.737953353109347 29.067921301459144 29.793774938681782 20.400350406749723 52-53 20.77169321255231 29.866037126586164 28.946534353711613 20.415735307149912 54-55 20.80482154699099 30.43731767248251 28.319039539056835 20.438821241469665 56-57 20.75184512644766 31.042689751741502 27.792265849189018 20.413199272621817 58-59 20.742739063016707 31.54622851509471 27.352795539570216 20.358236882318366 60-61 20.72230088439837 31.984851708067648 26.927542421855126 20.365304985678858 62-63 20.720362401956322 32.430064387147596 26.567708437294158 20.281864773601928 64-65 20.74089326643376 32.851477585719664 26.186951113863678 20.220678033982903 66-67 20.74153824456111 33.17437932115475 25.834658423430966 20.249424010853172 68-69 20.754527585256778 33.50482388294709 25.521512917009247 20.219135614786882 70-71 20.74617417270182 33.80150704560344 25.2529889313805 20.19932985031425 72-73 20.781164599959702 33.999713179100816 24.94443719903356 20.27468502190592 74-75 20.86125937424582 34.16788807556969 24.724909717369943 20.24594283281455 76-77 20.747680929433816 34.434683104146785 24.37803303478789 20.439602931631505 78-79 20.881693463600232 34.408437499172756 24.154198821176518 20.555670216050494 80-81 20.98144213556185 34.41230853061607 23.92224165981763 20.684007674004455 82-83 21.07581401113737 34.375013359458976 23.709295982033083 20.839876647370577 84-85 21.145076876732503 34.293491722020555 23.46678158366373 21.094649817583214 86-87 21.232373169604116 34.252501160802495 23.205598958416537 21.30952671117685 88-89 21.36034115313761 34.15576089345899 22.97583029012503 21.508067663278368 90-91 21.404573365681973 34.12195185403982 22.759354940102426 21.714119840175776 92-93 21.46456816059864 34.131211702560925 22.52494387314761 21.87927626369283 94-95 21.56076577177201 34.119157737711795 22.356696063453878 21.96338042706231 96-97 21.52442852434173 34.227956454636164 22.09123543474277 22.156379586279336 98-99 21.614196801281103 34.2692452869691 21.958166076596992 22.1583918351528 100 22.15915079910485 33.90407078150952 21.3392750423634 22.597503377022228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3655.0 1 2630.5 2 1574.0 3 1559.5 4 1776.5 5 2172.5 6 2644.0 7 3241.5 8 4260.0 9 5981.5 10 8658.0 11 13249.5 12 20893.5 13 35948.5 14 60459.0 15 84242.0 16 230080.5 17 280500.0 18 176230.0 19 154757.0 20 158847.5 21 176034.5 22 198665.0 23 226676.0 24 258662.5 25 296837.0 26 341252.5 27 388346.0 28 442635.5 29 500093.0 30 554037.0 31 608937.0 32 667031.5 33 717679.5 34 758883.0 35 807826.0 36 867543.5 37 931222.0 38 984579.0 39 1019533.0 40 1051040.5 41 1086987.5 42 1143208.5 43 1202170.5 44 1240860.5 45 1247969.5 46 1225924.0 47 1186819.5 48 1134077.5 49 1079437.0 50 1015022.5 51 949720.0 52 874526.5 53 780989.0 54 696741.0 55 632477.5 56 569720.5 57 508019.0 58 459613.0 59 410753.5 60 352570.0 61 306403.0 62 268972.5 63 229983.0 64 195369.0 65 165416.5 66 141987.5 67 119132.0 68 96404.0 69 78904.0 70 66562.0 71 58638.0 72 53530.5 73 49021.5 74 43818.5 75 34705.5 76 26221.0 77 22657.5 78 20621.5 79 16139.0 80 10057.5 81 5770.5 82 3327.5 83 1822.5 84 1085.5 85 680.0 86 477.5 87 309.5 88 187.0 89 127.5 90 99.5 91 74.5 92 48.0 93 30.0 94 19.0 95 19.5 96 17.0 97 9.0 98 6.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11579076604012468 2 0.008784336921766915 3 0.0024341771864958544 4 0.0015426597919417477 5 0.020450131088048298 6 0.010625183419054404 7 0.007606803707799545 8 0.00967889703780414 9 0.00869609799875644 10-11 0.007043900233422379 12-13 0.008909088502574828 14-15 0.005461685062200073 16-17 0.005638162908221023 18-19 0.0035675909389579867 20-21 0.014576157264885488 22-23 0.011827058404886733 24-25 0.0023413741812607 26-27 0.018151355007551273 28-29 0.015852578927054252 30-31 0.005595564807457346 32-33 0.006776140742907834 34-35 0.01620097053687147 36-37 0.004609723046926525 38-39 0.005638162908221023 40-41 0.0013585751422129989 42-43 0.002872329080065108 44-45 0.00433892083492886 46-47 1.551787956391107E-4 48-49 8.991241982619061E-4 50-51 2.4493907939114536E-4 52-53 1.5974287786379044E-4 54-55 5.431257847368875E-4 56-57 4.731431906251317E-4 58-59 0.007796973800494533 60-61 2.784090157054634E-4 62-63 0.0011288496702374525 64-65 0.011624717426259265 66-67 0.0036436589760359818 68-69 4.305450898614543E-4 70-71 0.01035438120705674 72-73 0.0048713970944748285 74-75 0.0018393251365459299 76-77 0.002275955669373624 78-79 5.294335380628483E-4 80-81 0.0018134620039394116 82-83 7.454667633643553E-5 84-85 0.003088362305366615 86-87 0.006919148652614466 88-89 5.826811640174452E-4 90-91 0.0013996518822351161 92-93 9.128164449359454E-5 94-95 0.004968764181934662 96-97 0.0015441811526833076 98-99 0.011133317906735414 100 0.006216279990013789 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.2865315E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.48442163010077 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81514750742083 35.06046252558694 2 11.588210088857556 10.30989647061854 3 3.8354737500786182 5.118564943490431 4 1.760475075574408 3.1325486212454194 5 1.0138400975815671 2.2550045183160465 6 0.6202984213859875 1.6556169908052107 7 0.4123293880170065 1.283956404292096 8 0.2907296649304611 1.034635279611564 9 0.22025856199068974 0.8818267265310176 >10 1.1653828966119242 9.487511624174864 >50 0.1091699295718381 3.4427045534723866 >100 0.1545930357013625 14.739208225436018 >500 0.010329298773819861 3.155371210305284 >1k 0.0034495493735110542 2.428676540935797 >5k 9.517995277818455E-5 0.3234643998373471 >10k+ 2.1755417752856247E-4 5.690550965341102 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 415219 1.2633957714995279 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 370191 1.1263881085576086 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132045 0.40177615823855634 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84034 0.25569205711249077 No Hit ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55726 0.16955869736833495 No Hit CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52498 0.1597367924208242 No Hit TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT 52382 0.15938383672878229 No Hit ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51899 0.15791420225243544 No Hit TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45803 0.13936577209133702 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45188 0.13749449837921832 No Hit TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44930 0.13670947623657342 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40333 0.12272208557867162 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38513 0.11718433247939355 No Hit GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36057 0.10971140851685128 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 35301 0.10741111107561269 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.085442966239636E-6 0.0 2 3.042721483119818E-6 6.085442966239636E-6 0.0 9.128164449359454E-6 6.085442966239636E-6 3 3.042721483119818E-6 6.085442966239636E-6 0.0 1.521360741559909E-5 6.085442966239636E-6 4 3.042721483119818E-6 9.128164449359454E-6 0.0 1.8256328898718908E-5 6.085442966239636E-6 5 9.128164449359454E-6 1.2170885932479272E-5 0.0 1.8256328898718908E-5 9.128164449359454E-6 6 9.128164449359454E-6 1.521360741559909E-5 0.0 1.8256328898718908E-5 1.521360741559909E-5 7 9.128164449359454E-6 1.521360741559909E-5 0.0 1.8256328898718908E-5 1.521360741559909E-5 8 9.128164449359454E-6 1.521360741559909E-5 0.0 2.4341771864958545E-5 2.1299050381838726E-5 9 1.2170885932479272E-5 1.8256328898718908E-5 0.0 2.738449334807836E-5 2.1299050381838726E-5 10-11 1.6734968157159E-5 2.1299050381838726E-5 0.0 3.194857557275809E-5 3.9555379280557634E-5 12-13 4.411946150523736E-5 3.042721483119818E-5 0.0 4.868354372991709E-5 4.868354372991708E-5 14-15 1.2475158080791255E-4 3.042721483119818E-5 0.0 5.629034743771663E-5 5.781170817927654E-5 16-17 3.301352809185002E-4 3.3469936314318E-5 0.0 6.541851188707608E-5 6.237579040395627E-5 18-19 7.272104344656365E-4 4.259810076367745E-5 0.0 7.454667633643555E-5 6.6939872628636E-5 20-21 0.001390523717785757 4.411946150523736E-5 0.0 8.671756226891482E-5 6.998259411175581E-5 22-23 0.0024676471228101725 5.172626521303691E-5 0.0 9.432436597671436E-5 7.758939781955535E-5 24-25 0.003896204859134927 5.933306892083645E-5 0.0 9.88884482013941E-5 8.367484078579499E-5 26-27 0.005393223828829877 6.085442966239636E-5 0.0 1.0801661265075354E-4 8.823892301047472E-5 28-29 0.009411137547289597 6.389715114551618E-5 0.0 1.1258069487543326E-4 9.280300523515446E-5 30-31 0.01250406393488089 6.846123337019591E-5 0.0 1.1714477710011298E-4 9.88884482013941E-5 32-33 0.012803772000968194 6.998259411175581E-5 0.0 1.2627294154947245E-4 1.0801661265075354E-4 34-35 0.013241923894537447 6.998259411175581E-5 0.0 1.3692246674039182E-4 1.1714477710011298E-4 36-37 0.013816998254847094 7.454667633643555E-5 0.0 1.3692246674039182E-4 1.2627294154947245E-4 38-39 0.014632447612323206 8.367484078579499E-5 0.0 1.4605063118975126E-4 1.3083702377415216E-4 40-41 0.015517879563911072 9.128164449359454E-5 0.0 1.5974287786379044E-4 1.3692246674039182E-4 42-43 0.016836899326843513 9.128164449359454E-5 0.0 1.6734968157158998E-4 1.4148654896507155E-4 44-45 0.018633626362625763 9.128164449359454E-5 0.0 1.8256328898718906E-4 1.4300790970663146E-4 46-47 0.02091110339274095 9.128164449359454E-5 0.0 1.9625553566122826E-4 1.4300790970663146E-4 48-49 0.023698236271278705 9.736708745983418E-5 0.0 2.023409786274679E-4 1.4300790970663146E-4 50-51 0.02723692135614705 1.00409808942954E-4 0.0 2.1299050381838727E-4 1.5670015638067063E-4 52-53 0.031583448994783714 1.019311696845139E-4 0.0 2.2364002900930661E-4 1.6278559934691024E-4 54-55 0.03702839908882663 1.0345253042607382E-4 0.0 2.3276819345866608E-4 1.719137637962697E-4 56-57 0.044072299322249 1.0345253042607382E-4 0.0 2.3733227568334582E-4 1.7647784602094945E-4 58-59 0.05259952627869229 1.0345253042607382E-4 0.0 2.4341771864958543E-4 1.7647784602094945E-4 60-61 0.0632886068488922 1.0497389116763373E-4 0.0 2.4341771864958543E-4 1.7647784602094945E-4 62-63 0.07646663359228414 1.1105933413387334E-4 0.0 2.555886045820647E-4 1.7799920676250935E-4 64-65 0.0928273470070194 1.1410205561699317E-4 0.0 2.6623812977298405E-4 1.8104192824562918E-4 66-67 0.11291995832080112 1.2170885932479271E-4 0.0 2.7384493348078364E-4 1.8256328898718908E-4 68-69 0.13742603714584814 1.2475158080791255E-4 0.0 2.784090157054634E-4 1.84084649728749E-4 70-71 0.16703932398031177 1.2475158080791255E-4 0.0 2.8601581941326286E-4 1.9169145343654853E-4 72-73 0.2032370601042467 1.2931566303259228E-4 0.0 2.966653446041822E-4 1.9473417491966836E-4 74-75 0.24691076291220698 1.3235838451571209E-4 0.0 3.0883623053666153E-4 1.9473417491966836E-4 76-77 0.2983053714835838 1.3844382748195172E-4 0.0 3.1340031276134127E-4 1.9929825714434807E-4 78-79 0.3587125210879616 1.4605063118975126E-4 0.0 3.1492167350290114E-4 2.00819617885908E-4 80-81 0.4298330930344042 1.4909335267287107E-4 0.0 3.194857557275809E-4 2.0538370011058773E-4 82-83 0.5121934781394915 1.5061471341443097E-4 0.0 3.225284772107007E-4 2.0994778233526747E-4 84-85 0.6076284374575445 1.521360741559909E-4 0.0 3.377420846262998E-4 2.1603322530150708E-4 86-87 0.7185036869416892 1.521360741559909E-4 0.0 3.4991297055877904E-4 2.1907594678462688E-4 88 0.8117676644815363 1.521360741559909E-4 0.0 3.559984135250187E-4 2.221186682677467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 70500 0.0 79.63911 1 TATCTCG 71015 0.0 78.61202 2 ATATGGA 83955 0.0 70.32299 1 TATGGAT 87110 0.0 67.43319 2 ATGGATC 101540 0.0 57.801304 3 ATCTCGA 101185 0.0 54.974976 3 TGGATCC 111405 0.0 52.48073 4 GGATCCG 112170 0.0 51.72918 5 TCTCGAG 109555 0.0 50.474823 4 CTCGAGG 122860 0.0 44.836792 5 GATCCGG 136530 0.0 42.406746 6 TCGAGGG 139415 0.0 39.438915 6 ATCCGGC 149935 0.0 38.3486 7 CGAGGGC 156155 0.0 35.15356 7 TCCGGCG 164580 0.0 34.844795 8 GAGGGCG 172825 0.0 31.716553 8 AGGGCGC 178515 0.0 30.580614 9 CCGGCGC 205545 0.0 27.75733 9 CCGTCGA 189525 0.0 16.937366 16-17 CGCCGTC 191755 0.0 16.812294 14-15 >>END_MODULE