FastQCFastQC Report
Wed 25 May 2016
SRR445721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36528347
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT3915401.071879874553316No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3434300.9401739421715415No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1131720.30981965868863437No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT814900.22308701787135343No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551110.150871869455248No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT509530.13948892896796014No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT500360.13697854983692528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493260.1350348538903225No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA449700.1231098686179257No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA436730.11955920151546962No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435150.11912666072735238No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA426400.1167312607931588No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410560.11239490251228725No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA395000.10813519702930986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC730200.079.239321
TATCTCG730400.078.6266252
ATATGGA852350.068.965811
TATGGAT876000.066.76022
ATCTCGA1020800.056.010253
ATGGATC1088900.053.7420163
TCTCGAG1103850.051.5328334
TGGATCC1183650.049.2420544
GGATCCG1181000.048.9589655
CTCGAGG1219150.046.4745255
TCGAGGG1416200.039.8799556
GATCCGG1457600.039.5792056
CGAGGGC1541450.036.466867
ATCCGGC1600550.035.7546657
TCCGGCG1736350.032.8717588
GAGGGCG1720700.032.572498
AGGGCGC1827950.030.6140989
CCGGCGC2235550.025.4822719
CCGTCGA1944650.016.62006616-17
CGCCGTC1968800.016.4884514-15
Produced by FastQC (version 0.11.5)