##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445721_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36528347
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.112451652958725	34.0	31.0	34.0	30.0	34.0
2	32.37077240314214	34.0	31.0	34.0	30.0	34.0
3	32.44111560810567	34.0	31.0	34.0	30.0	34.0
4	35.756249030376324	37.0	37.0	37.0	35.0	37.0
5	35.58624448568669	37.0	37.0	37.0	35.0	37.0
6	35.552948426601404	37.0	37.0	37.0	35.0	37.0
7	35.51759875693253	37.0	36.0	37.0	35.0	37.0
8	35.47860846262767	37.0	36.0	37.0	35.0	37.0
9	37.21422965019468	39.0	38.0	39.0	35.0	39.0
10-11	37.143634367030074	39.0	38.0	39.0	35.0	39.0
12-13	37.09005589549399	39.0	38.0	39.0	34.0	39.0
14-15	38.48510525264119	41.0	39.0	41.0	34.5	41.0
16-17	38.472289247033274	41.0	39.0	41.0	35.0	41.0
18-19	38.426119377917644	41.0	39.0	41.0	34.0	41.0
20-21	38.360450186809715	41.0	39.0	41.0	34.0	41.0
22-23	38.292361176376254	41.0	39.0	41.0	34.0	41.0
24-25	38.208873672821824	41.0	39.0	41.0	34.0	41.0
26-27	37.94944757560478	40.0	38.0	41.0	34.0	41.0
28-29	37.69488323684617	40.0	38.0	41.0	33.0	41.0
30-31	37.43757893013883	40.0	37.0	41.0	33.0	41.0
32-33	37.08087068653832	40.0	35.5	41.0	32.5	41.0
34-35	36.842644686330864	40.0	35.0	41.0	32.0	41.0
36-37	36.55584124844193	40.0	35.0	41.0	31.0	41.0
38-39	36.18251462350596	40.0	35.0	41.0	30.0	41.0
40-41	35.77856479517126	40.0	35.0	41.0	28.0	41.0
42-43	35.520201543749025	40.0	35.0	41.0	26.0	41.0
44-45	35.41422472525242	40.0	35.0	41.0	24.5	41.0
46-47	35.11575959076386	40.0	35.0	41.0	22.0	41.0
48-49	34.72054939962106	40.0	35.0	41.0	16.5	41.0
50-51	34.3816104791164	40.0	35.0	41.0	10.0	41.0
52-53	34.12122858994961	39.0	35.0	41.0	7.0	41.0
54-55	33.84529952860993	39.0	34.5	41.0	2.0	41.0
56-57	33.55881450096825	39.0	34.0	41.0	2.0	41.0
58-59	33.25649232088164	38.0	34.0	41.0	2.0	41.0
60-61	32.937179459557804	37.5	33.5	41.0	2.0	41.0
62-63	32.54054185643824	37.0	33.0	40.5	2.0	41.0
64-65	32.159259656616825	36.5	33.0	40.0	2.0	41.0
66-67	31.748684631144137	36.0	32.5	40.0	2.0	41.0
68-69	31.288608009007362	35.0	32.0	39.0	2.0	41.0
70-71	30.7498690811276	35.0	31.0	39.0	2.0	41.0
72-73	30.26026938749788	35.0	31.0	38.5	2.0	40.5
74-75	29.74976451849847	35.0	30.0	37.0	2.0	40.0
76-77	28.921549283355198	34.5	29.0	36.0	2.0	39.0
78-79	28.75825048968134	35.0	29.0	36.0	2.0	39.0
80-81	28.276071046959775	35.0	28.5	36.0	2.0	38.5
82-83	27.77453848376988	35.0	27.0	35.5	2.0	37.0
84-85	27.27152920442855	35.0	25.5	35.0	2.0	37.0
86-87	26.746999378318435	34.0	24.0	35.0	2.0	36.5
88-89	26.277470631233328	34.0	21.0	35.0	2.0	36.0
90-91	25.80083321317551	34.0	16.0	35.0	2.0	36.0
92-93	25.295834930608823	34.0	4.5	35.0	2.0	35.5
94-95	24.836059855103763	34.0	2.0	35.0	2.0	35.0
96-97	24.31110297709338	33.5	2.0	35.0	2.0	35.0
98-99	23.799739405125557	33.0	2.0	35.0	2.0	35.0
100	22.956825831730082	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.289508970400405
1101	2	-0.2506651455538105
1101	3	-0.21117663602625214
1101	4	-0.16753288521327647
1101	5	-0.21283818573725455
1101	6	-0.19537943814894732
1101	7	-0.1799447478332752
1101	8	-0.19100849830120126
1101	9	-0.2709771434485262
1101	10-11	-0.2442576292087537
1101	12-13	-0.26170806911597566
1101	14-15	-0.3373180044547226
1101	16-17	-0.3424394155786601
1101	18-19	-0.30590830918507095
1101	20-21	-0.3224190331937322
1101	22-23	-0.27608708113963587
1101	24-25	-0.3425774895056293
1101	26-27	-0.29474710303575336
1101	28-29	-0.3080890240265788
1101	30-31	-0.3712416008900945
1101	32-33	-0.4065943723497085
1101	34-35	-0.3634024425723865
1101	36-37	-0.39035310486762853
1101	38-39	-0.44056945366431677
1101	40-41	-0.3932251517537253
1101	42-43	-0.39754593765279367
1101	44-45	-0.45660269843413914
1101	46-47	-0.477310162754776
1101	48-49	-0.4178052954203153
1101	50-51	-0.4426806996412864
1101	52-53	-0.4939157858444858
1101	54-55	-0.48965863887479344
1101	56-57	-0.4734624341082707
1101	58-59	-0.484269785605008
1101	60-61	-0.48201134666488343
1101	62-63	-0.5583983688393985
1101	64-65	-0.5194101734612104
1101	66-67	-0.5771952856054448
1101	68-69	-0.6260344181525852
1101	70-71	-0.6192516920811961
1101	72-73	-0.6957166609769772
1101	74-75	-0.6483804998528804
1101	76-77	-0.6283357327036683
1101	78-79	-0.6127040648516484
1101	80-81	-0.5344563071831168
1101	82-83	-0.444436766487577
1101	84-85	-0.4645409662286255
1101	86-87	-0.5293978296868005
1101	88-89	-0.6118681847541758
1101	90-91	-0.5519330984152333
1101	92-93	-0.6102989411750244
1101	94-95	-0.6472748065801603
1101	96-97	-0.6613370253285673
1101	98-99	-0.6782513623741337
1101	100	-0.6423549648466533
1102	1	-0.04779898250508552
1102	2	-0.045185713785073744
1102	3	-0.04802328122417521
1102	4	-0.02181524226444509
1102	5	-0.012481444939020037
1102	6	-1.7904709412164266E-4
1102	7	-0.018685986057541015
1102	8	-0.001799423451601001
1102	9	0.004183819196818206
1102	10-11	-0.006778739654798471
1102	12-13	-0.029775226691221235
1102	14-15	-0.046733889709585696
1102	16-17	-0.04655848116185979
1102	18-19	-0.030175470482824096
1102	20-21	-0.06330602439850708
1102	22-23	-0.0713616327644786
1102	24-25	-0.07523283597390673
1102	26-27	-0.08597990713118975
1102	28-29	-0.07773785028030034
1102	30-31	-0.08060905149105224
1102	32-33	-0.1286375171115708
1102	34-35	-0.14505854113780714
1102	36-37	-0.11969209377814849
1102	38-39	-0.12565793708365192
1102	40-41	-0.17232035781623267
1102	42-43	-0.16367989481868506
1102	44-45	-0.18520419259441212
1102	46-47	-0.22460243108474742
1102	48-49	-0.1963779550932685
1102	50-51	-0.17431012177655703
1102	52-53	-0.15146538525686282
1102	54-55	-0.14946702274257717
1102	56-57	-0.21108874969360159
1102	58-59	-0.14841556408896395
1102	60-61	-0.1956092967961709
1102	62-63	-0.18585782483421553
1102	64-65	-0.17252665683668056
1102	66-67	-0.166764303736624
1102	68-69	-0.21081929153629986
1102	70-71	-0.19666627958986638
1102	72-73	-0.17143779573262208
1102	74-75	-0.22454896963891358
1102	76-77	-0.17524251280379843
1102	78-79	-0.22816776164442842
1102	80-81	-0.2006820288035378
1102	82-83	-0.145049182242321
1102	84-85	-0.22903662703372873
1102	86-87	-0.2569512419905955
1102	88-89	-0.2523552105004647
1102	90-91	-0.1873313962607206
1102	92-93	-0.21922858842008353
1102	94-95	-0.2392873747933244
1102	96-97	-0.2774400847970817
1102	98-99	-0.30547355795569686
1102	100	-0.30361614731839737
1103	1	0.03291053652620235
1103	2	0.04208649689235244
1103	3	0.035720155858491864
1103	4	0.05801727258291578
1103	5	0.08863975480231545
1103	6	0.08773108109384253
1103	7	0.05955566440314897
1103	8	0.04041712586067092
1103	9	0.05749007202134493
1103	10-11	0.07911194982071379
1103	12-13	0.12100030723264155
1103	14-15	0.12107561074176232
1103	16-17	0.07992548846582537
1103	18-19	0.1013517287165584
1103	20-21	0.09007445749350751
1103	22-23	0.09414650808115255
1103	24-25	0.08362820088916578
1103	26-27	0.08230029038894315
1103	28-29	0.0817349202472073
1103	30-31	0.06034698062882171
1103	32-33	0.06386330522464334
1103	34-35	0.07936419981523812
1103	36-37	0.08403356521438354
1103	38-39	0.04722034179217616
1103	40-41	0.08279359474821035
1103	42-43	0.1223192338692769
1103	44-45	0.0417388529924807
1103	46-47	0.0403277460741549
1103	48-49	0.007628267575320535
1103	50-51	0.03714845096602204
1103	52-53	0.022971046210741974
1103	54-55	0.0014781174165179323
1103	56-57	-0.0046236814648779045
1103	58-59	0.004555619633102026
1103	60-61	-0.01675432006513944
1103	62-63	-0.019740081953031563
1103	64-65	0.049488103925767746
1103	66-67	0.05180493212182924
1103	68-69	0.06462211373174398
1103	70-71	0.10775166618299536
1103	72-73	0.10973142204058917
1103	74-75	0.028163343802901863
1103	76-77	0.060776910036540954
1103	78-79	0.04387510676180284
1103	80-81	0.07610332784852503
1103	82-83	0.11943429641362968
1103	84-85	0.06936930810453745
1103	86-87	0.0619651713332523
1103	88-89	0.01857814463420837
1103	90-91	0.09668746525472471
1103	92-93	0.04171772808034291
1103	94-95	0.001070682714964022
1103	96-97	-9.15066739999304E-5
1103	98-99	-0.13411024551867357
1103	100	-0.09638803703377263
1104	1	0.06864424880124886
1104	2	0.043989796817221816
1104	3	0.06781232742409316
1104	4	0.05149732762782122
1104	5	0.060428983916366974
1104	6	0.06038922943655933
1104	7	0.06111559146561518
1104	8	0.0960681001088588
1104	9	0.04512972039849927
1104	10-11	0.044821029984362326
1104	12-13	0.08400308036133453
1104	14-15	0.10418765793104257
1104	16-17	0.12709057585194472
1104	18-19	0.1369648782144779
1104	20-21	0.1481343143744951
1104	22-23	0.14454438034702122
1104	24-25	0.13429340769717157
1104	26-27	0.1409110545008545
1104	28-29	0.13622248049600927
1104	30-31	0.13593977189309925
1104	32-33	0.10180177021077697
1104	34-35	0.09526225569504021
1104	36-37	0.09692595795500836
1104	38-39	0.13194176591039763
1104	40-41	0.08567093980580864
1104	42-43	0.1010849031803076
1104	44-45	0.09989395308123505
1104	46-47	0.01518174199510014
1104	48-49	0.02649467408276962
1104	50-51	0.05523981069834605
1104	52-53	-0.01487164794580309
1104	54-55	0.022858616057639836
1104	56-57	0.05467127583165876
1104	58-59	0.06270831513634789
1104	60-61	-0.009817781948797233
1104	62-63	-0.048661302706342724
1104	64-65	-0.09342753863673181
1104	66-67	-0.04521767434593471
1104	68-69	-0.09096146768387214
1104	70-71	-0.007603225816438908
1104	72-73	-1.3264304543980643E-4
1104	74-75	0.0029534029773081727
1104	76-77	0.058582916359409154
1104	78-79	-0.010402694686710845
1104	80-81	-0.04275915057491275
1104	82-83	0.04758101230199685
1104	84-85	-0.0038567749756879266
1104	86-87	-0.04366290181692278
1104	88-89	-0.10256525124215443
1104	90-91	-0.05883808709653238
1104	92-93	-0.0709053046363195
1104	94-95	-0.029003234379576526
1104	96-97	-0.0020465168795631428
1104	98-99	-0.1192046691356019
1104	100	-0.07210866206552424
1105	1	0.07030562776522231
1105	2	0.08030294827335638
1105	3	0.10078601006524224
1105	4	0.10317538254270175
1105	5	0.11377413423809202
1105	6	0.10840104851833132
1105	7	0.12010195922058386
1105	8	0.12260278957341342
1105	9	0.11839829705413507
1105	10-11	0.13362617458585646
1105	12-13	0.16760555696433954
1105	14-15	0.17581687443644256
1105	16-17	0.17157730527109294
1105	18-19	0.14248594038908635
1105	20-21	0.14639364360160556
1105	22-23	0.2018550592404864
1105	24-25	0.16514610282571596
1105	26-27	0.18536306073999498
1105	28-29	0.12980669688474933
1105	30-31	0.1560348759369674
1105	32-33	0.15738144422800104
1105	34-35	0.16633915205132865
1105	36-37	0.15057244150848703
1105	38-39	0.1667664270463618
1105	40-41	0.1587101092203227
1105	42-43	0.1204834425842023
1105	44-45	0.18639652228894477
1105	46-47	0.18926137471947868
1105	48-49	0.15993826280341494
1105	50-51	0.1787806308926818
1105	52-53	0.16606749561068312
1105	54-55	0.1670269879070645
1105	56-57	0.19292884992847092
1105	58-59	0.12143722095760268
1105	60-61	0.11506075547116978
1105	62-63	0.13792205221292875
1105	64-65	0.18336583065476475
1105	66-67	0.14502645606912523
1105	68-69	0.14347183995511514
1105	70-71	0.05397185334432564
1105	72-73	0.1774610981202258
1105	74-75	0.1654696989803277
1105	76-77	0.15977885591951235
1105	78-79	0.1735336214840082
1105	80-81	0.15578824185846685
1105	82-83	0.2159336231871336
1105	84-85	0.18141688539736123
1105	86-87	0.1333828493320368
1105	88-89	0.0906510070306723
1105	90-91	0.164885961724746
1105	92-93	0.1425617044356713
1105	94-95	0.12383449889023268
1105	96-97	0.12593233409682725
1105	98-99	0.0963686995073374
1105	100	0.024719161439481496
1106	1	0.02439308606825108
1106	2	-0.003140479813552588
1106	3	0.019891287819788772
1106	4	0.054534503144537894
1106	5	0.06300546614209424
1106	6	0.04546215024531364
1106	7	0.06909753936357532
1106	8	0.08467846115928523
1106	9	0.06244185985148931
1106	10-11	0.06940840696055517
1106	12-13	0.09626098263521499
1106	14-15	0.09375620045475586
1106	16-17	0.11888022783328012
1106	18-19	0.11325336807596642
1106	20-21	0.06905253551963852
1106	22-23	0.09353095914601539
1106	24-25	0.0920587032174609
1106	26-27	0.0980025150592212
1106	28-29	0.0975622031739718
1106	30-31	0.08741481115426808
1106	32-33	0.10276177006655729
1106	34-35	0.05574273507452432
1106	36-37	0.045131113142687695
1106	38-39	0.09781446873387267
1106	40-41	0.11123213176863089
1106	42-43	0.10270764889285999
1106	44-45	0.1177750248515963
1106	46-47	0.09975382632404717
1106	48-49	0.14443671472781716
1106	50-51	0.1440261670393923
1106	52-53	0.08593369464795586
1106	54-55	0.1589291278627698
1106	56-57	0.13638463999509298
1106	58-59	0.11316413944226156
1106	60-61	0.11056106428934953
1106	62-63	0.1435341960244969
1106	64-65	0.12726236609930197
1106	66-67	0.049515777743138756
1106	68-69	0.08346799558448481
1106	70-71	0.04415337572642031
1106	72-73	0.08919919753278194
1106	74-75	0.08263483908214653
1106	76-77	0.10716355044081638
1106	78-79	0.0887184213697374
1106	80-81	0.06229968010971376
1106	82-83	0.09432973186472893
1106	84-85	0.04271064654547274
1106	86-87	0.052199969595957185
1106	88-89	-0.02506024649720473
1106	90-91	0.059014270163505245
1106	92-93	0.031956589124249035
1106	94-95	0.002020686408485517
1106	96-97	-0.005121096586545804
1106	98-99	-0.06667350590040755
1106	100	-0.10494764201401807
1107	1	-6.318857100779951E-4
1107	2	0.0034842262563756776
1107	3	0.0019318513808954663
1107	4	0.0035219610848287175
1107	5	0.01332114528314321
1107	6	-0.012465820825994456
1107	7	-0.0065705368938040465
1107	8	0.009202705718649895
1107	9	-0.07896486794754054
1107	10-11	0.05220399035732015
1107	12-13	0.05399693976161046
1107	14-15	0.026485406457169347
1107	16-17	0.024931611969314815
1107	18-19	-0.007960536692330322
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2207	66-67	0.09336860620847887
2207	68-69	0.13181737937823002
2207	70-71	0.12590680517485353
2207	72-73	0.15390959401570115
2207	74-75	0.21074642874412675
2207	76-77	0.20036706777859692
2207	78-79	0.146336656498125
2207	80-81	0.08996557749887657
2207	82-83	0.06310287102521528
2207	84-85	0.08816696850409755
2207	86-87	0.11458580609932767
2207	88-89	0.10376062552291287
2207	90-91	0.013720319449539886
2207	92-93	0.03942263402805679
2207	94-95	0.08501425139377972
2207	96-97	0.1194070606760782
2207	98-99	0.1498826517828924
2207	100	0.16371617305794217
2208	1	-0.09507632423387236
2208	2	-0.11939538205653832
2208	3	-0.13430565220119206
2208	4	-0.08729937014344813
2208	5	-0.0851566205635379
2208	6	-0.12053067594604272
2208	7	-0.10991982068467365
2208	8	-0.11399731891692966
2208	9	-0.1466297096490834
2208	10-11	-0.19751902119428877
2208	12-13	-0.16051552690634452
2208	14-15	-0.1789401786516791
2208	16-17	-0.22369627032588113
2208	18-19	-0.18429820135263952
2208	20-21	-0.16158844788996163
2208	22-23	-0.11016565875389261
2208	24-25	-0.163742889862327
2208	26-27	-0.5953046145449932
2208	28-29	-0.28082416092912155
2208	30-31	-0.23973493889803166
2208	32-33	-0.2070638813016572
2208	34-35	-0.15903244956258078
2208	36-37	-0.08677071799338165
2208	38-39	-0.06310260415667557
2208	40-41	-0.05244616393228796
2208	42-43	-2.9586021270233687E-4
2208	44-45	-0.058806092219157335
2208	46-47	-0.031299818483958575
2208	48-49	-5.526297389124579E-4
2208	50-51	0.0637903295281248
2208	52-53	0.10751768430497322
2208	54-55	0.09105229711283869
2208	56-57	0.14190334486917777
2208	58-59	0.18789925289416942
2208	60-61	0.1599487534285231
2208	62-63	0.11515454881047305
2208	64-65	0.14942096076858036
2208	66-67	0.1848731351094557
2208	68-69	0.2281872899563666
2208	70-71	0.22129083726145282
2208	72-73	0.26964028246251814
2208	74-75	0.3162333357715106
2208	76-77	0.32227138728089955
2208	78-79	0.3238050901911933
2208	80-81	0.33580003123528357
2208	82-83	0.35899504166788176
2208	84-85	0.4375652133367325
2208	86-87	0.4741312245017113
2208	88-89	0.5344465352899732
2208	90-91	0.5400787203540496
2208	92-93	0.603836272188012
2208	94-95	0.6846555404346759
2208	96-97	0.7408167871416573
2208	98-99	0.8285885751974007
2208	100	0.7833291827156117
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	389171.0
3	132067.0
4	86686.0
5	70681.0
6	72283.0
7	88105.0
8	106264.0
9	110787.0
10	108568.0
11	105633.0
12	111799.0
13	158272.0
14	280637.0
15	425619.0
16	516683.0
17	440856.0
18	469871.0
19	386007.0
20	285044.0
21	275093.0
22	294333.0
23	330673.0
24	380307.0
25	442990.0
26	502886.0
27	560836.0
28	625421.0
29	676647.0
30	753536.0
31	843672.0
32	973541.0
33	1178128.0
34	1561559.0
35	2161118.0
36	3360146.0
37	5807909.0
38	8198157.0
39	3245217.0
40	11145.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.807173007345686	31.298517158206334	24.735695099221576	21.1586147352264
2	23.39128862941065	27.40382772510016	29.903646470485285	19.301237175003905
3	23.362639053974007	28.335170262213467	26.627093326216862	21.675097357595668
4	23.937135508527415	25.07411688776554	29.06077635443166	21.92797124927538
5	25.703645526873025	25.60004166388401	24.685114709440274	24.01119809980269
6	26.41618138809303	25.0816536745235	25.384333676063292	23.11783126132018
7	24.10146952564245	27.013337083039566	24.69756494690508	24.187628444412905
8	25.042427206658967	24.846628916297778	26.20152603513007	23.909417841913182
9	24.5361547253632	25.87013605412563	24.786906615530114	24.80680260498105
10-11	26.08391099693559	24.860820974663714	24.904902580363235	24.150365448037462
12-13	24.716770487439206	24.950492143508594	25.4364547848961	24.8962825841561
14-15	23.756520624473044	25.04829033824859	26.73118218895858	24.464006848319787
16-17	23.496499054822486	25.090861130326765	27.362911958488983	24.049727856361763
18-19	22.751072367639523	25.334598388165258	28.980824949317547	22.93350429487767
20-21	21.23416927130622	25.581051019976776	31.405299511461692	21.77948019725531
22-23	20.95366656439502	25.436347455563446	32.20075701095121	21.40922896909033
24-25	20.044864932270734	24.491359435098268	34.085686052517	21.378089580114004
26-27	20.0101206280178	24.53150065321888	35.758972761458615	19.699405957304705
28-29	20.013912822625258	24.632857400604863	35.73488763690613	19.61834213986375
30-31	19.99469615066283	24.678757853433368	35.639012069152415	19.687533926751385
32-33	20.01824021487446	24.784265668247794	35.52771225765355	19.66978185922419
34-35	20.047462885275547	24.89221008064304	35.463593990674	19.596733043407404
36-37	20.090908547096166	25.02818733586704	35.18257797807261	19.698326138964184
38-39	20.209805072178526	25.279913852611923	34.76088202677757	19.74939904843198
40-41	20.327624192815737	25.760684782033643	34.04707821728516	19.864612807865456
42-43	20.479616547240116	26.256715069827223	33.210779668726076	20.05288871420658
44-45	20.638671248024373	26.932141044722417	32.282391908292134	20.14679579896107
46-47	20.74394797060314	27.633033882746606	31.311090779133743	20.311927367516518
48-49	21.000485887917428	28.425628562483958	30.071951846545875	20.501933703052742
50-51	21.03707271151612	29.423245559012145	28.891637113510548	20.648044615961183
52-53	21.118318568337955	30.048348855737267	28.131129705156543	20.702202870768232
54-55	21.179296161995456	30.500697195453625	27.575395957453125	20.744610685097793
56-57	21.122828946303994	31.012479052812196	27.136773306455815	20.727918694427995
58-59	21.14285274670825	31.401045004876117	26.75763933586573	20.6984629125499
60-61	21.138818585615244	31.77945836441658	26.387454915031515	20.694268134936657
62-63	21.129322177772497	32.145457209419945	26.103703275010716	20.621517337796842
64-65	21.192052454307536	32.48066463237688	25.780065787191486	20.547217126124096
66-67	21.20602243014439	32.719480067794386	25.493306086941477	20.581191415119743
68-69	21.220167258734396	32.98241218968459	25.237261231371477	20.560159320209536
70-71	21.221162423772917	33.24456233182095	24.976036292349225	20.55823895205691
72-73	21.277672960150333	33.368034713517524	24.736711702123436	20.617580624208706
74-75	21.389691067214834	33.46609998318533	24.536954527179457	20.607254422420382
76-77	21.26707497138989	33.707366880229344	24.206494737880117	20.81906341050065
78-79	21.404557391949925	33.62904679175103	24.033615709093187	20.932780107205858
80-81	21.510138194738303	33.622946306137294	23.809784733797738	21.05713076532667
82-83	21.58023523787117	33.60366983387292	23.619600494980578	21.196494433275326
84-85	21.61945621388207	33.54223972949358	23.37190292836445	21.466401128259896
86-87	21.684686384091435	33.53821128284089	23.116767276109197	21.66033505695848
88-89	21.78178417549404	33.49430771738858	22.913456854221142	21.81045125289624
90-91	21.807189394327644	33.53430946439436	22.7000324577622	21.958468683515793
92-93	21.820326024394387	33.6429443977596	22.45874938764497	22.07798019020104
94-95	21.880390956121335	33.7155590693561	22.276930975940395	22.12711899858218
96-97	21.815117548540684	33.9099253852433	22.01723779346892	22.257719272747103
98-99	21.893189425639623	34.01348122964077	21.87573650267134	22.217592842048266
100	22.430791199465347	33.72092924568813	21.210855284600747	22.637424270245777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4589.0
1	3337.0
2	2014.0
3	2001.0
4	2216.0
5	2664.5
6	3438.0
7	4295.0
8	5176.5
9	6599.5
10	8478.0
11	11836.5
12	18084.0
13	29679.5
14	48067.5
15	66916.0
16	181825.0
17	204067.0
18	122948.5
19	133959.5
20	154740.5
21	181098.0
22	209871.0
23	244750.5
24	285055.5
25	331689.0
26	386376.0
27	445507.0
28	512483.0
29	584939.5
30	652267.5
31	717252.0
32	778530.0
33	826322.5
34	865726.0
35	914167.5
36	977075.0
37	1042719.5
38	1101769.5
39	1139305.0
40	1168006.0
41	1194452.5
42	1227167.5
43	1274603.5
44	1315902.0
45	1325506.0
46	1317564.5
47	1287219.5
48	1225974.0
49	1168571.0
50	1102852.0
51	1041658.0
52	981432.0
53	891817.0
54	797005.0
55	725621.5
56	660176.0
57	593745.5
58	545245.0
59	494130.0
60	424858.5
61	372177.0
62	329804.5
63	284401.0
64	244397.5
65	207141.5
66	177818.5
67	149577.5
68	121980.5
69	101327.0
70	85749.5
71	76225.0
72	70690.5
73	66229.0
74	60415.0
75	48511.0
76	37536.0
77	32953.5
78	29651.5
79	22755.5
80	14203.5
81	8297.5
82	4764.5
83	2467.5
84	1407.0
85	881.0
86	584.0
87	417.0
88	281.0
89	199.5
90	138.5
91	88.0
92	57.0
93	41.5
94	34.0
95	24.5
96	25.0
97	19.0
98	11.5
99	7.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12140434386477986
2	0.014068526013509452
3	0.0024474143327646333
4	0.0015659071569813986
5	0.020553900235343252
6	0.010854583701802877
7	0.007717294187990495
8	0.011016102097365643
9	0.00930236454444544
10-11	0.00796094058129704
12-13	0.011897609273148878
14-15	0.008715149360577417
16-17	0.005857095039093885
18-19	0.006553814219953616
20-21	0.01735364592326064
22-23	0.012869457246450269
24-25	0.0025514431299067542
26-27	0.01888396428121973
28-29	0.01621617315450929
30-31	0.005599760646163375
32-33	0.00673860221487712
34-35	0.016192903555148554
36-37	0.004786693468499957
38-39	0.005773598241387709
40-41	0.0014221831609297843
42-43	0.0029661895185128418
44-45	0.004436280678126497
46-47	6.241727828527253E-4
48-49	0.001027968771759642
50-51	0.0014303959607041622
52-53	7.802159785659067E-4
54-55	5.790023840936465E-4
56-57	4.7634238691392195E-4
58-59	0.007861018184042107
60-61	2.655471927048875E-4
62-63	0.0011935935672095976
64-65	0.011541721282925832
66-67	0.003917505492378289
68-69	4.106399887188983E-4
70-71	0.010524702910865361
72-73	0.005316419053947336
74-75	0.0019847599454746747
76-77	0.0028826927208066653
78-79	8.377055769865523E-4
80-81	0.0018889439481069319
82-83	1.2319199661566947E-4
84-85	0.003564355102080037
86-87	0.007164299003182378
88-89	0.001045763171270794
90-91	0.002395399934193573
92-93	8.582375764224973E-4
94-95	0.005187751857482081
96-97	0.0016042335559284958
98-99	0.011987950070667035
100	0.006154124630933888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.6528347E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.94991005413275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07956032838244	35.877487741554056
2	11.667931408754702	10.722807975001382
3	4.027055211048714	5.55128474192145
4	1.8825409177236487	3.460103433705048
5	1.0776501179746352	2.4758962995380016
6	0.7020617771905262	1.9355805308609562
7	0.45406368405757935	1.4604929808905747
8	0.338952329133263	1.245986322904979
9	0.24482838835308707	1.012485818117037
>10	1.2796056145935062	10.767103642712643
>50	0.12278855611353708	3.9868079523535473
>100	0.10917917624780489	9.072450465753095
>500	0.007596135223549477	2.4792627666797262
>1k	0.005807942656558926	4.361388854420121
>5k	1.5964279578610402E-4	0.4681616432454979
>10k+	2.1876975107857103E-4	5.122698830341773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	391540	1.071879874553316	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	343430	0.9401739421715415	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113172	0.30981965868863437	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81490	0.22308701787135343	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55111	0.150871869455248	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50953	0.13948892896796014	No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50036	0.13697854983692528	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49326	0.1350348538903225	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	44970	0.1231098686179257	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	43673	0.11955920151546962	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43515	0.11912666072735238	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	42640	0.1167312607931588	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41056	0.11239490251228725	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	39500	0.10813519702930986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.475199849585309E-6	0.0	0.0	0.0	5.475199849585309E-6
2	5.475199849585309E-6	0.0	0.0	0.0	5.475199849585309E-6
3	5.475199849585309E-6	2.7375999247926547E-6	0.0	2.7375999247926547E-6	8.212799774377965E-6
4	8.212799774377965E-6	5.475199849585309E-6	0.0	8.212799774377965E-6	1.3687999623963274E-5
5	1.642559954875593E-5	1.0950399699170619E-5	0.0	1.0950399699170619E-5	1.3687999623963274E-5
6	1.642559954875593E-5	1.3687999623963274E-5	0.0	1.3687999623963274E-5	1.3687999623963274E-5
7	1.642559954875593E-5	1.3687999623963274E-5	0.0	1.642559954875593E-5	1.3687999623963274E-5
8	1.9163199473548582E-5	1.9163199473548582E-5	0.0	1.642559954875593E-5	1.642559954875593E-5
9	2.1900799398341238E-5	2.7375999247926548E-5	0.0	1.9163199473548582E-5	1.642559954875593E-5
10-11	2.7375999247926548E-5	3.9695198909493496E-5	0.0	2.7375999247926548E-5	2.3269599360737565E-5
12-13	4.5170398759078806E-5	4.5170398759078806E-5	0.0	3.695759898470084E-5	3.832639894709717E-5
14-15	1.149791968412915E-4	5.6120798458249427E-5	0.0	4.9276798646267785E-5	5.3383198533456765E-5
16-17	2.641783927424912E-4	6.0227198345438406E-5	0.0	6.022719834543841E-5	6.0227198345438406E-5
18-19	6.447047822886702E-4	6.980879808221271E-5	0.0	7.117759804460903E-5	6.980879808221271E-5
20-21	0.0014057575613810281	8.349679770617598E-5	0.0	7.254639800700536E-5	7.665279789419433E-5
22-23	0.002619883128026571	9.44471974053466E-5	0.0	7.939039781898699E-5	8.623439763096863E-5
24-25	0.0040927118875650195	9.855359729253557E-5	0.0	8.623439763096863E-5	9.718479733013925E-5
26-27	0.005747591042102179	1.0402879714212088E-4	0.0	9.170959748055394E-5	1.0402879714212088E-4
28-29	0.010613674908421123	1.0676639706691354E-4	0.0	1.0676639706691354E-4	1.0402879714212088E-4
30-31	0.014413463604033328	1.095039969917062E-4	0.0	1.149791968412915E-4	1.149791968412915E-4
32-33	0.014793989993579507	1.1908559672848049E-4	0.0	1.2729839650285846E-4	1.2182319665327314E-4
34-35	0.015241587581283106	1.2182319665327314E-4	2.7375999247926547E-6	1.3551119627723643E-4	1.3277359635244376E-4
36-37	0.015819221165414357	1.300359964276511E-4	2.7375999247926547E-6	1.4783039593880336E-4	1.3824879620202905E-4
38-39	0.016454344347966252	1.3277359635244376E-4	2.7375999247926547E-6	1.5878079563797398E-4	1.4098639612682173E-4
40-41	0.01713737552920202	1.437239960516144E-4	2.7375999247926547E-6	1.6836239537474827E-4	1.5193679582599232E-4
42-43	0.017992875505699724	1.491991959011997E-4	2.7375999247926547E-6	1.7246879526193725E-4	1.5741199567557767E-4
44-45	0.01925354027106674	1.601495956003703E-4	2.7375999247926547E-6	1.7794399511152258E-4	1.601495956003703E-4
46-47	0.020820816228010536	1.7246879526193725E-4	2.7375999247926547E-6	1.8068159503631522E-4	1.7246879526193725E-4
48-49	0.02264953297777203	1.7520639518672993E-4	2.7375999247926547E-6	1.957383946226748E-4	1.7246879526193725E-4
50-51	0.02504219531204081	1.847879949235042E-4	2.7375999247926547E-6	2.0121359447226015E-4	1.793127950739189E-4
52-53	0.028141158426906097	1.9710719458507117E-4	2.7375999247926547E-6	2.0668879432184545E-4	1.9163199473548584E-4
54-55	0.03195737272206706	2.053199943594491E-4	2.7375999247926547E-6	2.1627039405861973E-4	1.9847599454746748E-4
56-57	0.036820718988461204	2.1216399417143075E-4	2.7375999247926547E-6	2.2174559390820503E-4	2.0395119439705278E-4
58-59	0.04294883642010956	2.1627039405861973E-4	2.7375999247926547E-6	2.244831938329977E-4	2.0668879432184545E-4
60-61	0.0509891673992256	2.2037679394580872E-4	2.7375999247926547E-6	2.313271936449793E-4	2.1079519420903444E-4
62-63	0.0609376055259221	2.244831938329977E-4	2.7375999247926547E-6	2.4090879338175362E-4	2.2311439387060137E-4
64-65	0.07355657237925384	2.313271936449793E-4	2.7375999247926547E-6	2.4638399323133895E-4	2.354335935321683E-4
66-67	0.08938948154429216	2.3817119345696097E-4	2.7375999247926547E-6	2.5185919308092424E-4	2.5185919308092424E-4
68-69	0.10910156980276167	2.4501519326894264E-4	2.7375999247926547E-6	2.710223925544728E-4	2.655471927048875E-4
70-71	0.13343746433420597	2.545967930057169E-4	2.7375999247926547E-6	2.8334159221603977E-4	2.710223925544728E-4
72-73	0.16315684911775502	2.641783927424912E-4	2.7375999247926547E-6	2.9292319195281405E-4	2.8060399229124715E-4
74-75	0.1997352905128721	2.7102239255447286E-4	2.7375999247926547E-6	3.038735916519847E-4	3.01135991727192E-4
76-77	0.24369567010519255	2.751287924416618E-4	2.7375999247926547E-6	3.038735916519847E-4	3.1619279131355166E-4
78-79	0.296224737462114	2.7649759240405816E-4	2.7375999247926547E-6	3.066111915767773E-4	3.2303679112553326E-4
80-81	0.35815335416081107	2.8197279225364346E-4	2.7375999247926547E-6	3.0934879150157E-4	3.3124959089991124E-4
82-83	0.43090370336221345	2.8607919214083244E-4	2.7375999247926547E-6	3.3124959089991124E-4	3.380935907118929E-4
84-85	0.5156816978331924	2.956607918776067E-4	2.7375999247926547E-6	3.435687905614782E-4	3.421999905990819E-4
86-87	0.614756807911401	3.01135991727192E-4	2.7375999247926547E-6	3.531503902982525E-4	3.490439904110635E-4
88	0.6986272880073111	3.0934879150157E-4	2.7375999247926547E-6	3.586255901478378E-4	3.586255901478378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	73020	0.0	79.23932	1
TATCTCG	73040	0.0	78.626625	2
ATATGGA	85235	0.0	68.96581	1
TATGGAT	87600	0.0	66.7602	2
ATCTCGA	102080	0.0	56.01025	3
ATGGATC	108890	0.0	53.742016	3
TCTCGAG	110385	0.0	51.532833	4
TGGATCC	118365	0.0	49.242054	4
GGATCCG	118100	0.0	48.958965	5
CTCGAGG	121915	0.0	46.474525	5
TCGAGGG	141620	0.0	39.879955	6
GATCCGG	145760	0.0	39.579205	6
CGAGGGC	154145	0.0	36.46686	7
ATCCGGC	160055	0.0	35.754665	7
TCCGGCG	173635	0.0	32.871758	8
GAGGGCG	172070	0.0	32.57249	8
AGGGCGC	182795	0.0	30.614098	9
CCGGCGC	223555	0.0	25.482271	9
CCGTCGA	194465	0.0	16.620066	16-17
CGCCGTC	196880	0.0	16.48845	14-15
>>END_MODULE