FastQCFastQC Report
Wed 25 May 2016
SRR445720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25552876
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269040.10528756136882594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA177250.042.1984061
TATCTCG81650.035.939072
TCTCGAG214650.034.7245642
GATCCGG311500.027.3945621
CTCGAGG276800.026.902463
ATATCTC123050.024.3524651
ATCCGGC349200.024.3075642
TCGAGGG329250.022.5468834
TCCGGCG404350.021.0786723
GCGCCGT499600.016.9573369
CGAGGGC448250.016.5827715
CGGCGCG513500.016.5448655
CCGGCGC534200.015.9736054
TATGGAT177300.015.5692552
ATATGGA184500.015.1707041
AGGGCGC503350.014.6777937
GAGGGCG514700.014.4160176
CGTCGAC481250.014.25171412-13
GCCGTCG505550.013.72534510-11
TCGACTT511000.013.49893914-15
Produced by FastQC (version 0.11.5)