##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445720_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25552876
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76775479206333	33.0	31.0	34.0	30.0	34.0
2	32.01422579595346	34.0	31.0	34.0	30.0	34.0
3	32.09820350554669	34.0	31.0	34.0	30.0	34.0
4	35.474543413430254	37.0	35.0	37.0	33.0	37.0
5	35.25545402404019	37.0	35.0	37.0	33.0	37.0
6	35.13739494528913	37.0	35.0	37.0	32.0	37.0
7	35.095052118595184	37.0	35.0	37.0	32.0	37.0
8	35.05938877486824	37.0	35.0	37.0	32.0	37.0
9	36.71992506831716	39.0	37.0	39.0	33.0	39.0
10-11	36.656714962339265	39.0	37.0	39.0	33.0	39.0
12-13	36.59010312576948	39.0	37.0	39.0	33.0	39.0
14-15	37.851564360113514	40.0	38.0	41.0	33.0	41.0
16-17	37.7499790630221	40.0	38.0	41.0	32.0	41.0
18-19	37.65420170316641	40.0	38.0	41.0	32.0	41.0
20-21	37.54262708041162	40.0	38.0	41.0	32.0	41.0
22-23	37.39720773505104	40.0	38.0	41.0	32.0	41.0
24-25	37.2534051352967	40.0	38.0	41.0	31.5	41.0
26-27	36.96663430370812	40.0	37.0	41.0	31.0	41.0
28-29	36.83038887677458	40.0	37.0	41.0	30.5	41.0
30-31	36.64998258904399	40.0	36.5	41.0	30.0	41.0
32-33	36.647374663423406	40.0	36.0	41.0	30.0	41.0
34-35	36.47243815138461	40.0	36.0	41.0	30.0	41.0
36-37	36.33558872198965	40.0	36.0	41.0	30.0	41.0
38-39	36.09447678218295	40.0	36.0	41.0	28.5	41.0
40-41	35.76996918076854	40.0	35.0	41.0	27.0	41.0
42-43	35.397199301558075	40.0	35.0	41.0	24.5	41.0
44-45	34.98241460178494	39.5	35.0	41.0	23.5	41.0
46-47	34.60252734369313	39.0	35.0	41.0	20.0	41.0
48-49	34.15270733126087	39.0	34.0	41.0	14.5	41.0
50-51	33.80310572477243	39.0	34.0	41.0	9.0	41.0
52-53	33.52605538413758	38.5	33.0	40.5	7.5	41.0
54-55	33.075736484613316	38.0	33.0	40.0	2.0	41.0
56-57	32.77040079558951	38.0	32.5	40.0	2.0	41.0
58-59	32.359857947105446	38.0	32.0	40.0	2.0	41.0
60-61	31.893594873625965	37.0	31.0	40.0	2.0	41.0
62-63	31.486307842608404	36.5	31.0	40.0	2.0	41.0
64-65	30.97645803157343	36.0	30.0	39.5	2.0	41.0
66-67	30.448544030816727	35.5	29.0	39.0	2.0	41.0
68-69	29.874378054352864	35.0	28.5	39.0	2.0	40.0
70-71	29.33852758883188	35.0	28.0	38.0	2.0	40.0
72-73	28.79785688311562	34.5	26.5	37.5	2.0	40.0
74-75	28.1831938800157	34.0	26.0	37.0	2.0	39.0
76-77	27.406836416378336	33.5	25.5	36.0	2.0	39.0
78-79	27.464729469199476	34.0	25.5	36.0	2.0	39.0
80-81	27.02544169587799	34.0	24.5	35.5	2.0	37.5
82-83	26.48444216220515	33.5	22.5	35.0	2.0	37.0
84-85	25.87516432592558	33.0	19.5	35.0	2.0	36.5
86-87	25.283780952875908	33.0	16.5	35.0	2.0	36.0
88-89	24.766049680669994	32.5	7.5	35.0	2.0	36.0
90-91	24.14527120156651	32.0	2.0	35.0	2.0	35.0
92-93	23.429384171863866	31.5	2.0	35.0	2.0	35.0
94-95	22.79477705366707	31.0	2.0	34.0	2.0	35.0
96-97	22.095049085668478	31.0	2.0	34.0	2.0	35.0
98-99	21.34702770834876	30.0	2.0	34.0	2.0	35.0
100	20.21212344160399	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06366939456052378
1101	2	0.09810109145518453
1101	3	0.0699537758244233
1101	4	0.07604970722670146
1101	5	0.07932460796357788
1101	6	0.17300267952826687
1101	7	0.1684583140306657
1101	8	0.1636110853872239
1101	9	0.2153491570867132
1101	10-11	0.17798700802795508
1101	12-13	0.11890871553093518
1101	14-15	0.14165277910220908
1101	16-17	0.08161982541314217
1101	18-19	0.10704370316364731
1101	20-21	0.09147879726567965
1101	22-23	0.08350561011175728
1101	24-25	0.0794954323620729
1101	26-27	0.07685392491303134
1101	28-29	-0.01827993659443905
1101	30-31	-0.041572721467396434
1101	32-33	-0.04781446735469075
1101	34-35	-0.09817055557206089
1101	36-37	-0.05289078589915164
1101	38-39	-0.08317461190246433
1101	40-41	-0.10781133569545176
1101	42-43	-0.16843339401792434
1101	44-45	-0.22894450696503554
1101	46-47	-0.2686294598807777
1101	48-49	-0.2507787378762316
1101	50-51	-0.23759491451197334
1101	52-53	-0.2525962110409168
1101	54-55	-0.2965483191324765
1101	56-57	-0.2767557234035607
1101	58-59	-0.411304379311602
1101	60-61	-0.4225379705336607
1101	62-63	-0.45711884563671035
1101	64-65	-0.48807129669790683
1101	66-67	-0.5847261610026315
1101	68-69	-0.5361181093347511
1101	70-71	-0.5224824659527982
1101	72-73	-0.5873560925571333
1101	74-75	-0.6691710266677688
1101	76-77	-0.5075620298837116
1101	78-79	-0.4321724213625835
1101	80-81	-0.45759070793015155
1101	82-83	-0.4624855379114585
1101	84-85	-0.5182885769553245
1101	86-87	-0.6037802289806926
1101	88-89	-0.7156906498026743
1101	90-91	-0.8392526685226649
1101	92-93	-0.7534310056312492
1101	94-95	-0.7710165553244508
1101	96-97	-0.895155734413283
1101	98-99	-0.8916754579032471
1101	100	-0.7103960123448019
1102	1	0.16620785829976725
1102	2	0.16283912036529102
1102	3	0.11983923950538156
1102	4	0.14801979098782425
1102	5	0.16819013400672844
1102	6	0.20881006982975947
1102	7	0.23933941210641052
1102	8	0.20713526114214176
1102	9	0.22789353916226673
1102	10-11	0.25455557523243044
1102	12-13	0.23327322912926007
1102	14-15	0.2760862501244574
1102	16-17	0.24436519562178916
1102	18-19	0.2668963177862764
1102	20-21	0.27665872665325253
1102	22-23	0.2455406861951701
1102	24-25	0.23319128360991215
1102	26-27	0.1913222693261929
1102	28-29	0.18000490362893373
1102	30-31	0.18283263366529212
1102	32-33	0.13174893224461215
1102	34-35	0.0603553856892205
1102	36-37	0.0976092307556371
1102	38-39	0.08878369409416109
1102	40-41	0.010748394396323135
1102	42-43	0.049277119429781635
1102	44-45	-0.02651683242531533
1102	46-47	-0.027105146938318114
1102	48-49	0.013791088748703828
1102	50-51	0.06740837367021157
1102	52-53	-0.06280088170419162
1102	54-55	-0.007919979660094612
1102	56-57	0.0027558458686414156
1102	58-59	-0.0743823827018062
1102	60-61	-0.04942164823381745
1102	62-63	-0.06213493613252297
1102	64-65	-0.07902981279466914
1102	66-67	-0.13872709264459715
1102	68-69	-0.12377015139133718
1102	70-71	-0.17698602595351787
1102	72-73	-0.23943309219807318
1102	74-75	-0.342577386829376
1102	76-77	-0.26427139893072393
1102	78-79	-0.19843664237339098
1102	80-81	-0.20526851699744597
1102	82-83	-0.2644453329930272
1102	84-85	-0.3892193015433314
1102	86-87	-0.479493973616421
1102	88-89	-0.5455549259731107
1102	90-91	-0.6180083698489973
1102	92-93	-0.5076917921658364
1102	94-95	-0.5144458594847166
1102	96-97	-0.6016916206792509
1102	98-99	-0.6623928002094566
1102	100	-0.6682539856324041
1103	1	0.18740243226442388
1103	2	0.1898239126253216
1103	3	0.18591184256476367
1103	4	0.14138574754561972
1103	5	0.15015126415181612
1103	6	0.1974345505345667
1103	7	0.24095848122809826
1103	8	0.2157435641033345
1103	9	0.20020709894329514
1103	10-11	0.23254726291855832
1103	12-13	0.2529504091050967
1103	14-15	0.26357855233030136
1103	16-17	0.2351380290202627
1103	18-19	0.255195218158768
1103	20-21	0.318611705175627
1103	22-23	0.3321734479848004
1103	24-25	0.2929223839826278
1103	26-27	0.2614089119196592
1103	28-29	0.19848073651554898
1103	30-31	0.16861572070268238
1103	32-33	0.26758752142026054
1103	34-35	0.21585453142615307
1103	36-37	0.2421997240031999
1103	38-39	0.27135153686216995
1103	40-41	0.15901120539299995
1103	42-43	0.14939803887375547
1103	44-45	0.20224520832478987
1103	46-47	0.16253700770804613
1103	48-49	0.22084036336067925
1103	50-51	0.19603052868666992
1103	52-53	0.079552090322764
1103	54-55	0.257426519840088
1103	56-57	0.08504079653150143
1103	58-59	-0.021039106695198484
1103	60-61	0.03620292267194358
1103	62-63	0.06784407703320383
1103	64-65	0.012916886278521389
1103	66-67	-0.11196505515056643
1103	68-69	-0.04225365749467258
1103	70-71	-0.10382316025617655
1103	72-73	-0.14899774698953294
1103	74-75	-0.2695797603269483
1103	76-77	-0.2082946498807523
1103	78-79	-0.10208568406512697
1103	80-81	-0.11872814024207301
1103	82-83	-0.17084060153500857
1103	84-85	-0.22455327034820627
1103	86-87	-0.32190183543729134
1103	88-89	-0.5783417865158427
1103	90-91	-0.6871956428004999
1103	92-93	-0.6614788112661856
1103	94-95	-0.5418134215176913
1103	96-97	-0.4964314727825965
1103	98-99	-0.551298975697101
1103	100	-0.5292099725312411
1104	1	0.16012610961779217
1104	2	0.13478406604854598
1104	3	0.09717242888130073
1104	4	0.09587252466690188
1104	5	0.07563260875355837
1104	6	0.1463982726407238
1104	7	0.17516581952457955
1104	8	0.16349176405068278
1104	9	0.1797037002027011
1104	10-11	0.17947350851068222
1104	12-13	0.17457810746494573
1104	14-15	0.12473308415279405
1104	16-17	0.13181796193236295
1104	18-19	0.1454390466857447
1104	20-21	0.13121784927719204
1104	22-23	0.18951958180022643
1104	24-25	0.17213491352539734
1104	26-27	0.1303255648332282
1104	28-29	0.08992551328334741
1104	30-31	0.10065169970895482
1104	32-33	0.1724688712769833
1104	34-35	0.057456246240811026
1104	36-37	0.03759701133238025
1104	38-39	0.005086271688469424
1104	40-41	0.01590551564142828
1104	42-43	-0.015064688034584606
1104	44-45	0.02619249939689894
1104	46-47	-0.010858378225677257
1104	48-49	0.10348637176140585
1104	50-51	0.12077905710203396
1104	52-53	0.11166000583908442
1104	54-55	0.16904089667992395
1104	56-57	0.08908607685577863
1104	58-59	0.07221210120434307
1104	60-61	0.06891639185255016
1104	62-63	0.06970436087985732
1104	64-65	0.10136733019820454
1104	66-67	0.10175878366561264
1104	68-69	0.11448999992838793
1104	70-71	0.06279103697034216
1104	72-73	0.048519615996003296
1104	74-75	-0.019718822833194594
1104	76-77	0.0023186834097010944
1104	78-79	0.0461542179528216
1104	80-81	0.040292463097774345
1104	82-83	-0.0025517999579278694
1104	84-85	-0.010210743063275629
1104	86-87	-0.05810384224172438
1104	88-89	-0.1983552872011245
1104	90-91	-0.3176918299934677
1104	92-93	-0.26241653348871097
1104	94-95	-0.26735440640214847
1104	96-97	-0.28159178126733764
1104	98-99	-0.31517421166194026
1104	100	-0.2436876689582128
1105	1	0.014375849578929234
1105	2	0.015710564500167834
1105	3	0.02435615549828185
1105	4	0.041537803151086905
1105	5	0.09580002635151175
1105	6	0.11858832306968026
1105	7	0.12014713757681506
1105	8	0.12225908408372277
1105	9	0.13791936010213135
1105	10-11	0.09086258907966283
1105	12-13	0.11964614610639046
1105	14-15	0.07965009032479742
1105	16-17	0.1262871471552387
1105	18-19	0.17048172238106218
1105	20-21	0.15992368485259334
1105	22-23	0.21974220650265153
1105	24-25	0.16559069838653073
1105	26-27	0.15241568370520042
1105	28-29	0.12004880684394692
1105	30-31	0.06006406357357008
1105	32-33	0.059377455353548214
1105	34-35	-0.01668121986741511
1105	36-37	0.0027929159414625815
1105	38-39	0.07179284418891996
1105	40-41	0.006143652972163238
1105	42-43	0.05705685764158375
1105	44-45	0.08591724144724822
1105	46-47	0.03506237475222207
1105	48-49	0.09977404435669257
1105	50-51	0.10773916960771146
1105	52-53	0.12312770506157023
1105	54-55	0.14128048678243488
1105	56-57	0.1530999556083401
1105	58-59	0.06480868496431924
1105	60-61	0.07919105556028683
1105	62-63	0.06837230955729723
1105	64-65	0.07895943450861509
1105	66-67	0.009228817673566425
1105	68-69	0.05543043914569523
1105	70-71	0.11729508816071643
1105	72-73	0.1007336014071214
1105	74-75	0.12935385550517964
1105	76-77	0.09915639897367612
1105	78-79	0.09174406657178125
1105	80-81	0.021717185851091614
1105	82-83	-0.043182182733001895
1105	84-85	-0.009252673414323453
1105	86-87	-0.0829698704236641
1105	88-89	-0.23056512694022757
1105	90-91	-0.23932840133716482
1105	92-93	-0.184725092612954
1105	94-95	-0.12477968563375441
1105	96-97	-0.13886269287008446
1105	98-99	-0.19889407708427598
1105	100	-0.166796571909142
1106	1	-0.017051791701977237
1106	2	-0.027971003079418466
1106	3	-0.05677083151624629
1106	4	-0.02874099072376879
1106	5	-0.1036789049148581
1106	6	-0.054354164175251185
1106	7	-0.014788416941904359
1106	8	-0.0327823859450902
1106	9	-0.06884012404537998
1106	10-11	-0.05354916378287555
1106	12-13	-0.041622295634873296
1106	14-15	-0.10668885719191934
1106	16-17	-0.09801458187727974
1106	18-19	-0.12184114369442511
1106	20-21	0.022343719731878764
1106	22-23	0.019116042031996017
1106	24-25	-0.018075572841823373
1106	26-27	0.03866169407456255
1106	28-29	0.007906073571334105
1106	30-31	0.05796489584590603
1106	32-33	0.09313475680151129
1106	34-35	0.004391427803454917
1106	36-37	-0.009200832214574461
1106	38-39	0.06733737694805342
1106	40-41	0.06195929419169488
1106	42-43	0.022128435688394177
1106	44-45	0.04264487281029972
1106	46-47	0.07160825252292824
1106	48-49	0.10214414557404439
1106	50-51	0.10956780261548715
1106	52-53	0.06899869485540222
1106	54-55	0.1745201391572948
1106	56-57	0.1623433788417259
1106	58-59	0.13055173992809443
1106	60-61	0.2072754707946558
1106	62-63	0.2528888040066022
1106	64-65	0.11857973592897864
1106	66-67	0.10003669788774872
1106	68-69	0.24040444864296262
1106	70-71	0.22641893932290458
1106	72-73	0.28323351495108895
1106	74-75	0.2736644361288256
1106	76-77	0.20241523069570633
1106	78-79	0.25137557941590316
1106	80-81	0.3847896782350695
1106	82-83	0.36729764930168685
1106	84-85	0.4768552522065441
1106	86-87	0.32614646419505533
1106	88-89	0.28513524684780833
1106	90-91	0.2519376139624292
1106	92-93	0.3667652312818248
1106	94-95	0.3488365018793296
1106	96-97	0.39346575836119513
1106	98-99	0.3629773597647912
1106	100	0.356606327602492
1107	1	-0.12864745394228194
1107	2	-0.09368120918483669
1107	3	-0.14576171628055334
1107	4	-0.11268328436041486
1107	5	-0.10205671587358012
1107	6	-0.15475421260217814
1107	7	-0.17747692688089245
1107	8	-0.12950478515316632
1107	9	-0.10544682164024266
1107	10-11	-0.14248321530330088
1107	12-13	-0.10413098069398785
1107	14-15	-0.08176949642771802
1107	16-17	-0.14809265333612132
1107	18-19	-0.16426820070886095
1107	20-21	-0.12175021986254819
1107	22-23	-0.05892634121588003
1107	24-25	-0.07578720597690847
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2207	72-73	0.4231681717525504
2207	74-75	0.46429100559115
2207	76-77	0.4215417111880484
2207	78-79	0.3295323384684501
2207	80-81	0.3416599832624101
2207	82-83	0.41831978864449937
2207	84-85	0.4465430023468322
2207	86-87	0.5261511406181469
2207	88-89	0.5413646281781368
2207	90-91	0.561972851717556
2207	92-93	0.5917429440637285
2207	94-95	0.4834353911681504
2207	96-97	0.5115323485977754
2207	98-99	0.5239122325885468
2207	100	0.530638791809654
2208	1	-0.3598024237710824
2208	2	-0.4321329967852279
2208	3	-0.3465287323261421
2208	4	-0.3003903811802857
2208	5	-0.2898949064895078
2208	6	-0.44847389237563107
2208	7	-0.5149171549110534
2208	8	-0.5006156897308216
2208	9	-0.507067158318641
2208	10-11	-0.4966218937011817
2208	12-13	-0.46704075300038284
2208	14-15	-0.5162491609623814
2208	16-17	-0.4759839118358684
2208	18-19	-0.6359715605241121
2208	20-21	-0.7358964344450243
2208	22-23	-0.7246589243223696
2208	24-25	-0.38433180781012766
2208	26-27	-0.4100714106710228
2208	28-29	-0.21089419924901875
2208	30-31	-0.22576966961295142
2208	32-33	-0.4486534043385433
2208	34-35	-0.26456027919915215
2208	36-37	-0.20431725095016873
2208	38-39	-0.23358915422501525
2208	40-41	-0.2697230856053423
2208	42-43	-0.32836684512665926
2208	44-45	-0.1291285979679344
2208	46-47	-0.07041829053591897
2208	48-49	-0.2166523663946549
2208	50-51	-0.38522428076467463
2208	52-53	-0.20009419707606213
2208	54-55	-1.0892265019386898
2208	56-57	-0.3399801131507729
2208	58-59	-0.0779272855842379
2208	60-61	-0.180670052854758
2208	62-63	-0.1636164173968062
2208	64-65	0.025101433368522663
2208	66-67	0.2715060385636612
2208	68-69	-0.09667629674191147
2208	70-71	-0.12967379086658326
2208	72-73	0.30702101408590465
2208	74-75	0.5405883052232774
2208	76-77	0.5882074271091113
2208	78-79	0.5750476632111514
2208	80-81	0.35204041843413236
2208	82-83	0.43541071118834296
2208	84-85	0.4849241350119584
2208	86-87	0.92452371078112
2208	88-89	1.0537779796881352
2208	90-91	1.228002593982655
2208	92-93	0.9638272681563436
2208	94-95	1.4029777300823483
2208	96-97	1.1438238564360539
2208	98-99	1.2461623693977941
2208	100	1.2350001208895343
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	331968.0
3	160039.0
4	73083.0
5	70561.0
6	65177.0
7	71637.0
8	71719.0
9	81208.0
10	92576.0
11	101263.0
12	117357.0
13	158064.0
14	209979.0
15	274681.0
16	343455.0
17	333684.0
18	291598.0
19	254700.0
20	246094.0
21	254665.0
22	270453.0
23	290926.0
24	315062.0
25	346760.0
26	383798.0
27	432212.0
28	490778.0
29	561663.0
30	647483.0
31	752019.0
32	887941.0
33	1082823.0
34	1375977.0
35	1853356.0
36	2679457.0
37	4088624.0
38	4497732.0
39	991843.0
40	461.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.478975934938678	28.179256385308864	27.816729762027432	22.525037917725026
2	23.817142564546874	27.32954204919989	27.19141571700246	21.661899669250776
3	23.581126927090658	26.258200816682802	26.935078092524144	23.225594163702393
4	24.23917536072605	25.91728913054865	26.770441051509653	23.073094457215646
5	24.64482826277393	26.18892706085239	26.260443207467652	22.90580146890602
6	24.650537463553064	26.07516446876712	25.769916118005874	23.50438194967394
7	24.436257044273287	26.467931735874757	25.9527344922989	23.143076727553055
8	24.05930005271393	26.1896870261913	26.21056693949099	23.540445981603774
9	23.892592212952312	26.015009999916806	26.3421519293537	23.750245857777184
10-11	23.731610632618022	26.177310439454555	26.390579569699263	23.70049935822816
12-13	23.419221693553176	26.25241519669615	26.582217109444382	23.74614600030629
14-15	23.143535315459644	26.551740250357312	27.065426267100158	23.239298167082882
16-17	22.867276172244285	26.43121932852493	27.39014769157533	23.31135680765545
18-19	22.43931578608476	26.41648291022897	28.173962362632494	22.970238941053776
20-21	22.158608983170954	26.144607036716256	29.049875553666237	22.646908426446558
22-23	22.083985777262647	26.114970336027437	29.436857436300784	22.364186450409132
24-25	22.041321818634394	26.010466780465038	29.805157542117914	22.143053858782654
26-27	22.08195889991168	26.040395899678376	29.876536788789014	22.00110841162093
28-29	22.071937100830336	26.138106941875925	29.86607587049781	21.92388008679593
30-31	22.086210889460713	26.076566038962078	29.841974812689493	21.99524825888772
32-33	22.099683196826845	26.122597556302974	29.794879492376687	21.982839754493494
34-35	22.11573677959146	26.206920256748973	29.753459417121974	21.92388354653759
36-37	22.11730931214678	26.2214920148621	29.651957460909795	22.009241212081324
38-39	22.181176590551583	26.26344812571223	29.521008239594504	22.034367044141685
40-41	22.261628021532935	26.466957061816228	29.207949470275523	22.063465446375314
42-43	22.439745538722214	26.629736447894903	28.72642377309903	22.204094240283855
44-45	22.589105151996737	26.846534593825105	28.23582385734829	22.32853639682987
46-47	22.735464120317854	27.086068305010297	27.763819310623262	22.414648264048587
48-49	22.861677012569338	27.173117127549713	27.387795352382426	22.57741050749852
50-51	22.893996850223193	27.25760528000792	27.221056947609807	22.627340922159085
52-53	22.930516880128383	27.340648735659013	27.124069608149227	22.604764776063377
54-55	22.944465917242002	27.33837835662577	27.018036882447827	22.699118843684403
56-57	22.941774795508994	27.399325279860786	26.989349783016042	22.669550141614177
58-59	22.966775854550487	27.50286684009972	26.90302995937207	22.627327345977722
60-61	22.95937923388423	27.520199787227796	26.825943319705576	22.694477659182404
62-63	22.9686914144356	27.588240936712573	26.794331399630288	22.648736249221535
64-65	22.99648557313829	27.76491160602409	26.668204931744384	22.57039788909324
66-67	23.016812097324518	27.80404302715414	26.559717310365833	22.619427565155505
68-69	23.050599555322503	27.892020648550474	26.46524052711984	22.592139269007184
70-71	23.09246799403408	28.01957188464276	26.374012641654694	22.51394747966846
72-73	23.102584418865682	28.08967943776861	26.22842130626851	22.579314837097204
74-75	23.172978784464267	28.14227734114651	26.172150638492013	22.512593235897214
76-77	22.944906496088034	28.380229646481375	26.04732851053857	22.627535346892024
78-79	23.01323427383694	28.430882979267775	25.924330319600724	22.63155242729456
80-81	22.994580773920585	28.543810510276685	25.826950909549083	22.634657806253653
82-83	23.041400188172	28.671581072987458	25.690556322318486	22.596462416522062
84-85	23.042752905201507	28.735409213681002	25.560174094073517	22.661663787043977
86-87	23.023313745891343	28.833443862889602	25.422152784395745	22.721089606823313
88-89	23.06980892033856	28.934898354449924	25.313189156164146	22.682103569047367
90-91	23.103032358986745	29.00332143285475	25.125138574040662	22.768507634117842
92-93	23.103741896777144	29.094222661501632	24.977655812732085	22.824379628989142
94-95	23.166813131243853	29.208763124795155	24.80922148205141	22.81520226190958
96-97	23.179969970562873	29.270314576874558	24.640203351569774	22.909512100992803
98-99	23.18956387010625	29.34723311903665	24.47175521239518	22.991447798461913
100	23.84259983982798	29.201310336104513	23.632874993816657	23.323214830250848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3099.0
1	2227.0
2	1403.0
3	1599.0
4	2047.0
5	2636.0
6	3317.0
7	4020.5
8	4732.0
9	5586.5
10	6615.5
11	8038.5
12	10080.0
13	13442.0
14	20703.5
15	27166.0
16	33897.0
17	37397.0
18	37981.0
19	43925.0
20	52457.0
21	64261.5
22	79621.0
23	98058.5
24	119551.0
25	144976.5
26	174546.0
27	207133.0
28	243046.5
29	280959.5
30	316673.0
31	354316.5
32	398520.0
33	447492.0
34	500743.5
35	558945.5
36	622107.0
37	686787.5
38	750626.0
39	801683.0
40	840936.0
41	874829.5
42	897469.0
43	916268.5
44	929689.0
45	932776.0
46	926604.5
47	908018.5
48	880299.5
49	849346.5
50	817671.5
51	788230.5
52	755819.0
53	718377.5
54	680466.0
55	641194.5
56	596360.5
57	547846.5
58	500617.5
59	455506.5
60	411482.0
61	367749.0
62	325119.0
63	284917.0
64	249071.0
65	216523.0
66	186580.0
67	160363.5
68	135754.0
69	112408.5
70	92664.0
71	76776.5
72	64080.5
73	53612.5
74	44153.5
75	34827.0
76	27168.5
77	21782.5
78	17555.5
79	13514.0
80	9554.5
81	6338.0
82	4173.0
83	2719.5
84	1756.0
85	1126.5
86	718.5
87	456.5
88	288.0
89	178.0
90	113.5
91	71.0
92	41.5
93	20.5
94	10.5
95	7.5
96	5.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07554139894076894
2	0.03187508130200295
3	9.314020073513446E-4
4	0.03295910800803792
5	0.05161454233175162
6	0.2522220982092192
7	0.26260449117351803
8	0.2889811698690981
9	0.27654030019947656
10-11	0.2934072861309232
12-13	0.31430121603533
14-15	0.3337236873062743
16-17	0.3457790817753743
18-19	0.38523647983890347
20-21	0.4091789902631704
22-23	0.40828672279394307
24-25	0.40954098474081746
26-27	0.37815312843845833
28-29	0.33390370618164467
30-31	0.34232350205902456
32-33	0.3394862480450342
34-35	0.5477289523104953
36-37	0.5812437707598941
38-39	0.5986058085986093
40-41	0.5929293438437223
42-43	0.5948351958503615
44-45	0.5989697598031627
46-47	0.596110981793204
48-49	0.333526057888748
50-51	0.09797527292035543
52-53	0.10515646066611053
54-55	0.029881176584584845
56-57	0.044893185408953575
58-59	0.030386012126384518
60-61	0.019162226592419576
62-63	0.02598729004124624
64-65	0.018054719163510206
66-67	0.02233212418046407
68-69	0.011343145875243162
70-71	0.015984502096750283
72-73	0.009668187643535702
74-75	0.016593044164578578
76-77	0.011043766658594516
78-79	0.004807678008534147
80-81	0.011057463746937918
82-83	0.015301604406486377
84-85	0.01718006223643867
86-87	0.006421977706149398
88-89	0.011979082119758261
90-91	0.004314582828171671
92-93	0.0036336418648139648
94-95	0.0046628802174753245
96-97	0.0011055507020031718
98-99	0.002674845680775816
100	0.0013618819267154116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5552876E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.03764752767284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52460227375364	42.66771734665877
2	11.171579162347872	12.297148725295932
3	4.2166344271891	6.96220918070053
4	2.1251253064952227	4.678475902840871
5	1.267977504237259	3.489324947561427
6	0.7923070793970344	2.6164030661720328
7	0.5453086501973932	2.1008753698348612
8	0.4031097094462064	1.7748968082786334
9	0.292044298930281	1.4466088068292016
>10	1.5555548340442027	15.400171834150388
>50	0.08131422220818484	3.0020287784005237
>100	0.02366859165657455	2.3442549041377023
>500	4.602376959645963E-4	0.17244687553875343
>1k	1.8567960877084144E-4	0.1772356556089271
>5k	4.267426409682752E-5	0.1485390544988261
>10k+	8.534852817231316E-5	0.7216627434924984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26904	0.10528756136882594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.913453812400608E-6	0.0	0.0	7.826907624801216E-6	0.0
2	3.913453812400608E-6	1.5653815249602432E-5	0.0	1.5653815249602432E-5	0.0
3	7.826907624801216E-6	1.956726906200304E-5	0.0	1.956726906200304E-5	0.0
4	7.826907624801216E-6	2.7394176686804255E-5	0.0	2.348072287440365E-5	0.0
5	7.826907624801216E-6	3.5221084311605475E-5	0.0	2.7394176686804255E-5	0.0
6	7.826907624801216E-6	4.304799193640669E-5	0.0	3.1307630499204865E-5	0.0
7	7.826907624801216E-6	5.087489956120791E-5	0.0	3.1307630499204865E-5	0.0
8	7.826907624801216E-6	5.087489956120791E-5	0.0	3.913453812400608E-5	0.0
9	7.826907624801216E-6	5.087489956120791E-5	0.0	5.087489956120791E-5	0.0
10-11	7.826907624801216E-6	5.478835337360851E-5	0.0	5.870180718600912E-5	3.913453812400608E-6
12-13	7.826907624801216E-6	6.457198790461003E-5	0.0	7.239889552941125E-5	3.913453812400608E-6
14-15	7.826907624801216E-6	7.631234934181186E-5	0.0	9.196616459141429E-5	3.913453812400608E-6
16-17	1.9567269062003042E-5	8.413925696661307E-5	3.913453812400608E-6	1.0174979912241581E-4	3.913453812400608E-6
18-19	4.696144574880729E-5	8.609598387281337E-5	3.913453812400608E-6	1.0761997984101671E-4	3.913453812400608E-6
20-21	1.1153343365341732E-4	9.39228914976146E-5	3.913453812400608E-6	1.1153343365341732E-4	3.913453812400608E-6
22-23	2.7198503996184224E-4	1.0370652602861612E-4	3.913453812400608E-6	1.1936034127821854E-4	3.913453812400608E-6
24-25	4.402635538950684E-4	1.1349016055961763E-4	3.913453812400608E-6	1.3501415652782098E-4	5.870180718600912E-6
26-27	5.948449794848925E-4	1.1349016055961763E-4	3.913453812400608E-6	1.467545179650228E-4	7.826907624801216E-6
28-29	8.335656620413296E-4	1.1544688746581793E-4	3.913453812400608E-6	1.6436506012082553E-4	7.826907624801216E-6
30-31	9.685798185691506E-4	1.1740361437201824E-4	3.913453812400608E-6	1.8001887537042797E-4	9.783634531001521E-6
32-33	9.959739952559547E-4	1.2131706818441885E-4	3.913453812400608E-6	1.956726906200304E-4	1.5653815249602432E-5
34-35	0.0010566325293481643	1.2327379509061915E-4	3.913453812400608E-6	2.2306686730683465E-4	1.956726906200304E-5
36-37	0.001203387047313187	1.2914397580922007E-4	3.913453812400608E-6	2.387206825564371E-4	2.348072287440365E-5
38-39	0.00147928554108743	1.447977910588225E-4	3.913453812400608E-6	2.5633122471223986E-4	2.348072287440365E-5
40-41	0.001950856725481703	1.4871124487122312E-4	3.913453812400608E-6	2.6807158614944164E-4	2.348072287440365E-5
42-43	0.0026259275081208077	1.54581425589824E-4	3.913453812400608E-6	2.798119475866435E-4	2.348072287440365E-5
44-45	0.0036727764029379707	1.7023524083942644E-4	3.913453812400608E-6	3.0524939736724746E-4	2.348072287440365E-5
46-47	0.005197066662868007	1.7610542155802736E-4	3.913453812400608E-6	3.1503303189824895E-4	2.348072287440365E-5
48-49	0.007494264050747164	1.7610542155802736E-4	3.913453812400608E-6	3.2677339333545073E-4	2.7394176686804255E-5
50-51	0.010664161638791657	1.8393232918282858E-4	3.913453812400608E-6	3.2873012024165106E-4	2.935090359300456E-5
52-53	0.014520870370912455	2.0154287133863132E-4	7.826907624801216E-6	3.4242720858505317E-4	3.1307630499204865E-5
54-55	0.01968467267637506	2.3089377493163587E-4	7.826907624801216E-6	3.443839354912535E-4	3.5221084311605475E-5
56-57	0.0263747219686739	2.387206825564371E-4	1.1740361437201824E-5	3.522108431160547E-4	3.5221084311605475E-5
58-59	0.034953012725456034	2.465475901812383E-4	1.5653815249602432E-5	3.659079314594568E-4	3.913453812400608E-5
60-61	0.04583632777774212	2.4850431708743864E-4	1.5653815249602432E-5	3.756915659904584E-4	3.913453812400608E-5
62-63	0.05949232485611404	2.5437449780603953E-4	1.5653815249602432E-5	3.8156174670905926E-4	3.913453812400608E-5
64-65	0.0765530267512745	2.641581323370411E-4	1.5653815249602432E-5	3.9330210814626115E-4	4.109126503020638E-5
66-67	0.09812007071141424	2.739417668680426E-4	1.5653815249602432E-5	3.9917228886486204E-4	4.304799193640669E-5
68-69	0.12463959047114696	2.798119475866435E-4	1.5653815249602432E-5	4.1286937720826414E-4	4.500471884260699E-5
70-71	0.15690993060820238	2.915523090238453E-4	1.5653815249602432E-5	4.265664655516663E-4	4.69614457488073E-5
72-73	0.1975941964419191	3.032926704610472E-4	1.5653815249602432E-5	4.383068269888681E-4	5.08748995612079E-5
74-75	0.24660824871533052	3.09162851179648E-4	1.7610542155802737E-5	4.44177007707469E-4	6.457198790461003E-5
76-77	0.3059459921458547	3.1503303189824895E-4	1.956726906200304E-5	4.500471884260699E-4	6.652871481081034E-5
78-79	0.37699278938308156	3.2481666642925045E-4	2.348072287440365E-5	4.5983082295707146E-4	7.631234934181186E-5
80-81	0.46124553651025424	3.2873012024165106E-4	2.348072287440365E-5	4.676577305818727E-4	7.826907624801216E-5
82-83	0.5605631240882631	3.404704816788529E-4	2.7394176686804255E-5	4.7939809201907446E-4	8.609598387281337E-5
84-85	0.676215467879232	3.4634066239745383E-4	2.7394176686804255E-5	4.891817265500761E-4	9.000943768521398E-5
86-87	0.8098657857534315	3.5416757002225505E-4	3.1307630499204865E-5	4.891817265500761E-4	9.39228914976146E-5
88	0.9236611957104163	3.6395120455325655E-4	3.1307630499204865E-5	4.891817265500761E-4	9.39228914976146E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	17725	0.0	42.198406	1
TATCTCG	8165	0.0	35.93907	2
TCTCGAG	21465	0.0	34.724564	2
GATCCGG	31150	0.0	27.394562	1
CTCGAGG	27680	0.0	26.90246	3
ATATCTC	12305	0.0	24.352465	1
ATCCGGC	34920	0.0	24.307564	2
TCGAGGG	32925	0.0	22.546883	4
TCCGGCG	40435	0.0	21.078672	3
GCGCCGT	49960	0.0	16.957336	9
CGAGGGC	44825	0.0	16.582771	5
CGGCGCG	51350	0.0	16.544865	5
CCGGCGC	53420	0.0	15.973605	4
TATGGAT	17730	0.0	15.569255	2
ATATGGA	18450	0.0	15.170704	1
AGGGCGC	50335	0.0	14.677793	7
GAGGGCG	51470	0.0	14.416017	6
CGTCGAC	48125	0.0	14.251714	12-13
GCCGTCG	50555	0.0	13.725345	10-11
TCGACTT	51100	0.0	13.498939	14-15
>>END_MODULE