FastQCFastQC Report
Wed 25 May 2016
SRR445719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30690825
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327840.10682019789301851No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311690.101558038925314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA209100.046.480251
TCTCGAG252700.038.1870962
TATCTCG89150.031.966552
CTCGAGG323150.029.8094883
GATCCGG354950.028.1500721
TCGAGGG381000.025.2234
ATCCGGC401600.024.813142
TCCGGCG462500.021.5191863
ATATCTC145050.020.5943531
CGAGGGC514200.018.790415
GCGCCGT567650.017.4526799
CGGCGCG588300.016.9431255
AGGGCGC570000.016.839387
GAGGGCG583650.016.4271016
CCGGCGC616050.016.225134
TATGGAT214700.015.6828682
ATATGGA220000.015.5240891
GGGCGCG634900.015.1402928
CGTCGAC547700.014.55088812-13
GGATCCG239150.014.5121082
Produced by FastQC (version 0.11.5)