##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445719_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30690825
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810743503962502	33.0	31.0	34.0	30.0	34.0
2	32.05941808341744	34.0	31.0	34.0	30.0	34.0
3	32.14243230672359	34.0	31.0	34.0	30.0	34.0
4	35.51392899995357	37.0	35.0	37.0	33.0	37.0
5	35.295962164588275	37.0	35.0	37.0	33.0	37.0
6	35.176673777912455	37.0	35.0	37.0	33.0	37.0
7	35.13319293306713	37.0	35.0	37.0	33.0	37.0
8	35.09856701473486	37.0	35.0	37.0	33.0	37.0
9	36.767068985600744	39.0	37.0	39.0	33.0	39.0
10-11	36.705658319709556	39.0	37.0	39.0	33.0	39.0
12-13	36.64202744631335	39.0	37.0	39.0	33.0	39.0
14-15	37.91692821877548	40.0	38.0	41.0	33.0	41.0
16-17	37.816955865474455	40.0	38.0	41.0	33.0	41.0
18-19	37.71898050964742	40.0	38.0	41.0	32.0	41.0
20-21	37.61188848784612	40.0	38.0	41.0	32.0	41.0
22-23	37.4678579510326	40.0	38.0	41.0	32.0	41.0
24-25	37.32915441341183	40.0	38.0	41.0	32.0	41.0
26-27	37.051006383829694	40.0	37.0	41.0	31.0	41.0
28-29	36.92252684637836	40.0	37.0	41.0	31.0	41.0
30-31	36.749566816792964	40.0	37.0	41.0	30.0	41.0
32-33	36.749580648288216	40.0	37.0	41.0	30.0	41.0
34-35	36.578571201653915	40.0	36.5	41.0	30.0	41.0
36-37	36.4470623386631	40.0	36.0	41.0	30.0	41.0
38-39	36.21621738744396	40.0	36.0	41.0	29.5	41.0
40-41	35.91101190665289	40.0	35.5	41.0	27.5	41.0
42-43	35.55862975335462	40.0	35.0	41.0	25.5	41.0
44-45	35.16499644437711	40.0	35.0	41.0	23.5	41.0
46-47	34.79738443003732	39.0	35.0	41.0	22.0	41.0
48-49	34.35809425129497	39.0	34.5	41.0	17.0	41.0
50-51	34.01948724089365	39.0	34.0	41.0	13.5	41.0
52-53	33.75419359042971	39.0	33.5	41.0	8.5	41.0
54-55	33.31107467785568	38.0	33.0	40.0	6.0	41.0
56-57	33.01853294917944	38.0	33.0	40.0	2.0	41.0
58-59	32.61708634420874	38.0	32.0	40.0	2.0	41.0
60-61	32.160314344759385	37.0	31.5	40.0	2.0	41.0
62-63	31.764213001768447	37.0	31.0	40.0	2.0	41.0
64-65	31.26426607952051	36.0	30.5	40.0	2.0	41.0
66-67	30.748223288230278	35.5	29.5	39.0	2.0	41.0
68-69	30.179094566535763	35.0	29.0	39.0	2.0	40.5
70-71	29.651710942928382	35.0	28.0	38.5	2.0	40.0
72-73	29.11864391068015	35.0	27.5	37.5	2.0	40.0
74-75	28.509918664617196	34.0	26.0	37.0	2.0	39.5
76-77	27.731434932101045	33.5	26.0	36.0	2.0	39.0
78-79	27.79145332522016	34.0	26.0	36.0	2.0	39.0
80-81	27.355822774395932	34.0	25.5	36.0	2.0	38.0
82-83	26.81742818904347	34.0	24.5	35.0	2.0	37.0
84-85	26.211883649266515	33.0	21.0	35.0	2.0	37.0
86-87	25.62575595475195	33.0	19.0	35.0	2.0	36.0
88-89	25.112139637823354	33.0	13.5	35.0	2.0	36.0
90-91	24.49750182668599	32.0	4.5	35.0	2.0	35.5
92-93	23.785156964662892	32.0	2.0	35.0	2.0	35.0
94-95	23.163779109880558	31.0	2.0	34.5	2.0	35.0
96-97	22.473501624671215	31.0	2.0	34.0	2.0	35.0
98-99	21.737322603742324	31.0	2.0	34.0	2.0	35.0
100	20.594168094210566	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1064097521242573
1101	2	0.1249096894432924
1101	3	0.10704927077308213
1101	4	0.12332622850467345
1101	5	0.11588661746753104
1101	6	0.2113036264968784
1101	7	0.20119834077298293
1101	8	0.21437532547253824
1101	9	0.2628814017386105
1101	10-11	0.21493002960793461
1101	12-13	0.18123297398167182
1101	14-15	0.2086083397730718
1101	16-17	0.14365968008377905
1101	18-19	0.17295654852658515
1101	20-21	0.12737243460553316
1101	22-23	0.1375282753436764
1101	24-25	0.1381103031048454
1101	26-27	0.15106651906052804
1101	28-29	0.04707958175325189
1101	30-31	0.011566614918550044
1101	32-33	0.008654862131010077
1101	34-35	-0.041933105134681625
1101	36-37	-3.7426616595581663E-4
1101	38-39	-0.028263341236687722
1101	40-41	-0.07500792433652492
1101	42-43	-0.11828993009624611
1101	44-45	-0.1824591769830377
1101	46-47	-0.22185966844404703
1101	48-49	-0.21026279873063913
1101	50-51	-0.2048775872846278
1101	52-53	-0.20947989345772555
1101	54-55	-0.2657110739546198
1101	56-57	-0.25075437709905657
1101	58-59	-0.3762719424233367
1101	60-61	-0.3872081645240826
1101	62-63	-0.4298321651221819
1101	64-65	-0.4561141359097931
1101	66-67	-0.5300906693156406
1101	68-69	-0.5152045739069422
1101	70-71	-0.5174800383068465
1101	72-73	-0.6032569835673307
1101	74-75	-0.6939930925613211
1101	76-77	-0.5238841503157055
1101	78-79	-0.48082213988617184
1101	80-81	-0.5314194228656
1101	82-83	-0.5220232673234833
1101	84-85	-0.5979365201268436
1101	86-87	-0.6792374646632275
1101	88-89	-0.7595307429078311
1101	90-91	-0.9055623747357728
1101	92-93	-0.8531146195670338
1101	94-95	-0.8778550021104685
1101	96-97	-0.9721455318967998
1101	98-99	-0.9376814493542796
1101	100	-0.7675025314062403
1102	1	0.17296036319864427
1102	2	0.183615207388236
1102	3	0.13128905021807924
1102	4	0.1614424103973846
1102	5	0.18738464568648538
1102	6	0.2276275926361464
1102	7	0.26924042773135426
1102	8	0.22491859284064475
1102	9	0.22930309630065437
1102	10-11	0.2755787560680787
1102	12-13	0.24513209483842502
1102	14-15	0.28034866594851593
1102	16-17	0.24055004131125912
1102	18-19	0.25649617935662405
1102	20-21	0.2899886212668008
1102	22-23	0.2618304525008668
1102	24-25	0.2349727750163879
1102	26-27	0.2117244272925518
1102	28-29	0.17066248841644693
1102	30-31	0.18237270004226502
1102	32-33	0.11401906324729794
1102	34-35	0.04654241186386798
1102	36-37	0.09461474098878142
1102	38-39	0.05669937821611626
1102	40-41	-0.011872652912948922
1102	42-43	0.03234216294666936
1102	44-45	-0.06117751549868444
1102	46-47	-0.07240213819724772
1102	48-49	-0.022251649583921562
1102	50-51	0.020193058326121616
1102	52-53	-0.1145174326245737
1102	54-55	-0.07087883000498607
1102	56-57	-0.029229269933161106
1102	58-59	-0.12459060701818458
1102	60-61	-0.09862927642605257
1102	62-63	-0.10635564845945211
1102	64-65	-0.1112259642114779
1102	66-67	-0.16866895284474026
1102	68-69	-0.11460092708182046
1102	70-71	-0.1555995653114195
1102	72-73	-0.22368089703724436
1102	74-75	-0.3142934549806391
1102	76-77	-0.22980254827357527
1102	78-79	-0.16496634434889046
1102	80-81	-0.1661043253406156
1102	82-83	-0.25279835737502765
1102	84-85	-0.3769710588102697
1102	86-87	-0.4642794820525218
1102	88-89	-0.5404029126611576
1102	90-91	-0.6208194208549287
1102	92-93	-0.5075585098651239
1102	94-95	-0.5458762832157795
1102	96-97	-0.6503726983709655
1102	98-99	-0.6971690465054081
1102	100	-0.6936335737624688
1103	1	0.1493050218956249
1103	2	0.15415260324440538
1103	3	0.13301776641255003
1103	4	0.08800539705958244
1103	5	0.11521042192011777
1103	6	0.16691030890942926
1103	7	0.2066447416030428
1103	8	0.1641445095457783
1103	9	0.15596271038355525
1103	10-11	0.1866937253678813
1103	12-13	0.20987059393574725
1103	14-15	0.21089881122532006
1103	16-17	0.20449764199437226
1103	18-19	0.21218467328245794
1103	20-21	0.2686415906231332
1103	22-23	0.28670126255209283
1103	24-25	0.262802435893839
1103	26-27	0.23395277103155365
1103	28-29	0.16748945823453454
1103	30-31	0.12843021150122524
1103	32-33	0.20685274912722207
1103	34-35	0.14108046312753686
1103	36-37	0.1874655686436455
1103	38-39	0.23014499557478274
1103	40-41	0.09753310082324873
1103	42-43	0.0503563903990667
1103	44-45	0.08410309747084455
1103	46-47	0.031523218415763665
1103	48-49	0.09326222877081136
1103	50-51	0.0698411247293933
1103	52-53	-0.03283653153214772
1103	54-55	0.1351568947400068
1103	56-57	-0.019372968566123916
1103	58-59	-0.13145910085341228
1103	60-61	-0.08693047150912747
1103	62-63	-0.026695279675344352
1103	64-65	-0.0528790405250561
1103	66-67	-0.21395465694869742
1103	68-69	-0.15947281405859925
1103	70-71	-0.2532997108379753
1103	72-73	-0.2566736937412806
1103	74-75	-0.3190894309177139
1103	76-77	-0.28250655878419906
1103	78-79	-0.18388645762367517
1103	80-81	-0.20805170286386954
1103	82-83	-0.27541307725370956
1103	84-85	-0.3159246978073824
1103	86-87	-0.4172464304845711
1103	88-89	-0.639902618074796
1103	90-91	-0.7729530175762171
1103	92-93	-0.7943272353813775
1103	94-95	-0.6457020380751644
1103	96-97	-0.5728945222095412
1103	98-99	-0.5964091502032431
1103	100	-0.6095562803684693
1104	1	0.16920903507433138
1104	2	0.1672051532970329
1104	3	0.13150816789055142
1104	4	0.12592976138027012
1104	5	0.09790869299650495
1104	6	0.16620535685238025
1104	7	0.1917977222337015
1104	8	0.17118592323282655
1104	9	0.18294437633256422
1104	10-11	0.2047051514015763
1104	12-13	0.21600838253843335
1104	14-15	0.16190640083724617
1104	16-17	0.1799859339684602
1104	18-19	0.1942703502394636
1104	20-21	0.16780135753253944
1104	22-23	0.23504040106511326
1104	24-25	0.24593545393797456
1104	26-27	0.20075010245071923
1104	28-29	0.16769966491030175
1104	30-31	0.18006290961733384
1104	32-33	0.22331650027277306
1104	34-35	0.1305291858399329
1104	36-37	0.11502986179775121
1104	38-39	0.05280283675413244
1104	40-41	0.07837326282457013
1104	42-43	0.047694953207361834
1104	44-45	0.10073931011082493
1104	46-47	0.05818769383874667
1104	48-49	0.1828121623835557
1104	50-51	0.20961918789856782
1104	52-53	0.19939978893615518
1104	54-55	0.25425492709186415
1104	56-57	0.15885313519505218
1104	58-59	0.19206826440751712
1104	60-61	0.20964590686392626
1104	62-63	0.15645445966294247
1104	64-65	0.1702325244940326
1104	66-67	0.1502083141608601
1104	68-69	0.17316103523397786
1104	70-71	0.15127714816437532
1104	72-73	0.1313112073657301
1104	74-75	0.055299372957144044
1104	76-77	0.04161578720714232
1104	78-79	0.06973894757530985
1104	80-81	0.09303875924624094
1104	82-83	0.08209630928732992
1104	84-85	0.059516786869195926
1104	86-87	0.014822213745166124
1104	88-89	-0.12942315917662484
1104	90-91	-0.23159939888205727
1104	92-93	-0.18942727554550132
1104	94-95	-0.17263338226065272
1104	96-97	-0.19030461521558806
1104	98-99	-0.23838785394793405
1104	100	-0.16948861321703035
1105	1	0.013703803005380877
1105	2	0.006459087775915862
1105	3	0.02178490087224816
1105	4	0.03669181385630793
1105	5	0.08071365867634483
1105	6	0.11122356312977644
1105	7	0.10140398723488886
1105	8	0.0994990561344693
1105	9	0.1286550920229388
1105	10-11	0.09120597091470728
1105	12-13	0.10735785257200092
1105	14-15	0.06558166761080031
1105	16-17	0.09404769985368233
1105	18-19	0.12257668625200324
1105	20-21	0.1465440152783941
1105	22-23	0.1966909862247448
1105	24-25	0.16128744922215077
1105	26-27	0.14355576945170867
1105	28-29	0.10599458081119906
1105	30-31	0.05072214111816464
1105	32-33	0.07992267849252244
1105	34-35	-0.017978837561443584
1105	36-37	-0.0067539223106649615
1105	38-39	0.08379165222434892
1105	40-41	-0.0037443316478871225
1105	42-43	0.04685926738016377
1105	44-45	0.08034259664328403
1105	46-47	0.04317547490331464
1105	48-49	0.1094656647027108
1105	50-51	0.13726659352786896
1105	52-53	0.10418008870141904
1105	54-55	0.0915629850187969
1105	56-57	0.10968211979763964
1105	58-59	0.043662578129357144
1105	60-61	0.0453520130624554
1105	62-63	0.041328048206715096
1105	64-65	0.051205015978858626
1105	66-67	-0.02810949094184778
1105	68-69	0.06322036182522695
1105	70-71	0.11670110906003472
1105	72-73	0.09471096079720454
1105	74-75	0.10809965690803836
1105	76-77	0.09021371824671576
1105	78-79	0.09996272747542179
1105	80-81	0.04167496481655775
1105	82-83	-0.02105169752380931
1105	84-85	-0.011635586653149232
1105	86-87	-0.05725024316556926
1105	88-89	-0.17006413269698228
1105	90-91	-0.17479278633557271
1105	92-93	-0.06970884412633538
1105	94-95	-0.04144908802544478
1105	96-97	-0.04919982030698122
1105	98-99	-0.11639135691574154
1105	100	-0.08908256903111322
1106	1	-0.023249292205843375
1106	2	-0.025671361477918708
1106	3	-0.048630516138430835
1106	4	-0.021401789631852353
1106	5	-0.08791894612046036
1106	6	-0.03388197055604536
1106	7	-0.010440940138536803
1106	8	-0.023096049453343426
1106	9	-0.041269197638108324
1106	10-11	-0.03725093856264294
1106	12-13	-0.0022905638642782833
1106	14-15	-0.0753131080412146
1106	16-17	-0.05433177490753138
1106	18-19	-0.08056947448051233
1106	20-21	0.07262605815105871
1106	22-23	0.04935378717028982
1106	24-25	0.0155470375701654
1106	26-27	0.07700539240135384
1106	28-29	0.044138257930484315
1106	30-31	0.06883569045771765
1106	32-33	0.10776309932042238
1106	34-35	0.012683853385453858
1106	36-37	0.009649054207201857
1106	38-39	0.0969017439481874
1106	40-41	0.09340861079093798
1106	42-43	0.06377236588307511
1106	44-45	0.0813033539427579
1106	46-47	0.11984137713255194
1106	48-49	0.16005977283863615
1106	50-51	0.17568194637018308
1106	52-53	0.13670648598098012
1106	54-55	0.2424504763402382
1106	56-57	0.22135132558936732
1106	58-59	0.20423278964258174
1106	60-61	0.2670312493598601
1106	62-63	0.31368991479110164
1106	64-65	0.17404264321694285
1106	66-67	0.17258481522535263
1106	68-69	0.30560933808370905
1106	70-71	0.304447312174144
1106	72-73	0.33005699490549745
1106	74-75	0.32993842664998496
1106	76-77	0.24607619666478442
1106	78-79	0.28754209723044966
1106	80-81	0.40851799086096463
1106	82-83	0.39259557333986805
1106	84-85	0.482793210373444
1106	86-87	0.32824954024401976
1106	88-89	0.28143877413663176
1106	90-91	0.23474483499883902
1106	92-93	0.34110668158187707
1106	94-95	0.36912328246572557
1106	96-97	0.4144940708366889
1106	98-99	0.4551749288044462
1106	100	0.4301071919775481
1107	1	-0.13746810986197033
1107	2	-0.09652063731518368
1107	3	-0.1358831136638301
1107	4	-0.10248228243496271
1107	5	-0.100481104295568
1107	6	-0.1609297533800813
1107	7	-0.18597703331550974
1107	8	-0.1264203067145928
1107	9	-0.1263171115764905
1107	10-11	-0.15327647611845663
1107	12-13	-0.12029452587081124
1107	14-15	-0.09902415390232022
1107	16-17	-0.1704910830492068
1107	18-19	-0.20511027834773188
1107	20-21	-0.15284114496323298
1107	22-23	-0.0936925885083113
1107	24-25	-0.11707054240758197
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2207	72-73	0.47498304766346067
2207	74-75	0.5343711731000056
2207	76-77	0.4641590894177128
2207	78-79	0.33446390251912916
2207	80-81	0.3747061805750427
2207	82-83	0.43417018410440633
2207	84-85	0.4699027473525099
2207	86-87	0.5637386929444972
2207	88-89	0.5650018449399461
2207	90-91	0.5632719226710385
2207	92-93	0.5900381177879801
2207	94-95	0.49800666858126874
2207	96-97	0.5381206369661058
2207	98-99	0.5418277913792195
2207	100	0.503725432685048
2208	1	-0.3132799827213937
2208	2	-0.36532888220167337
2208	3	-0.28241010877846406
2208	4	-0.25625006516693816
2208	5	-0.2559009192250272
2208	6	-0.4070390417344214
2208	7	-0.4913878540267831
2208	8	-0.44195774018461975
2208	9	-0.45575713460544165
2208	10-11	-0.44184752918355485
2208	12-13	-0.4161603684811723
2208	14-15	-0.4531152656198074
2208	16-17	-0.41946381561212576
2208	18-19	-0.5828080626015364
2208	20-21	-0.6659770551105453
2208	22-23	-0.6632044005023943
2208	24-25	-0.330571919409401
2208	26-27	-0.37749099431776756
2208	28-29	-0.16538536241651514
2208	30-31	-0.16905832043174485
2208	32-33	-0.3866241232270724
2208	34-35	-0.20371744069255016
2208	36-37	-0.15799765232584662
2208	38-39	-0.19166765660055773
2208	40-41	-0.2410110103912544
2208	42-43	-0.28905156410109356
2208	44-45	-0.0875690575582837
2208	46-47	-0.03404967428731709
2208	48-49	-0.18712587813168113
2208	50-51	-0.37384236333928555
2208	52-53	-0.18097036097167063
2208	54-55	-1.0367157621705871
2208	56-57	-0.2911740860413303
2208	58-59	-0.05304311098070258
2208	60-61	-0.123048070468883
2208	62-63	-0.10345339869644121
2208	64-65	0.09291879282530147
2208	66-67	0.3072055760769743
2208	68-69	-0.069499793195817
2208	70-71	-0.10436512663022413
2208	72-73	0.33342635231191764
2208	74-75	0.5766394274785078
2208	76-77	0.6138156602533442
2208	78-79	0.5960148215334797
2208	80-81	0.40604745172841206
2208	82-83	0.45577439740785053
2208	84-85	0.5029142550669334
2208	86-87	0.9335176128038363
2208	88-89	1.0362781536483006
2208	90-91	1.1878287026967094
2208	92-93	0.9366904554409103
2208	94-95	1.3773478535235029
2208	96-97	1.1399612076898862
2208	98-99	1.2325547296887436
2208	100	1.2267738192028084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	388663.0
3	191625.0
4	85138.0
5	82461.0
6	75706.0
7	82983.0
8	82281.0
9	93019.0
10	105971.0
11	116452.0
12	135496.0
13	181871.0
14	242149.0
15	309133.0
16	388072.0
17	382109.0
18	337887.0
19	295600.0
20	285106.0
21	295479.0
22	314121.0
23	338830.0
24	367004.0
25	402273.0
26	447343.0
27	504098.0
28	572796.0
29	654505.0
30	757009.0
31	880165.0
32	1043207.0
33	1272544.0
34	1625185.0
35	2199534.0
36	3207355.0
37	4967343.0
38	5659418.0
39	1320203.0
40	691.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.27164856269287	28.473441686308888	28.154841182026296	22.10006856897195
2	23.538933609794054	27.586757498545033	27.40093331567211	21.4733755759888
3	23.31248555948803	26.479556405719062	27.187024599447774	23.020933435345135
4	23.91021048178878	26.201724268419934	27.0748622555905	22.813202994200786
5	24.31250783014661	26.496567384346843	26.54425161206887	22.64667317343768
6	24.300869860196187	26.398034869675165	26.05356275752268	23.24753251260597
7	24.11042536289836	26.812070829714678	26.25961991277593	22.817883894611033
8	23.73200973450506	26.5274870877414	26.49422848740134	23.2462746903522
9	23.59926800782888	26.35116920535443	26.64824530808559	23.4013174787311
10-11	23.383271894049372	26.52023030445858	26.687915731969408	23.408582069522634
12-13	23.090977171779777	26.587269988585238	26.889354261707975	23.43239857792701
14-15	22.83785012048035	26.827624820587264	27.371050059014117	22.96347499991826
16-17	22.60706162223862	26.73779592226718	27.614347649776466	23.04079480571773
18-19	22.206486397775652	26.72601261265184	28.356857225977798	22.710643763594714
20-21	21.931971996273678	26.443856146874793	29.157230220993647	22.466941635857882
22-23	21.856661634284354	26.42303202662024	29.554812730708456	22.16549360838695
24-25	21.828630607839887	26.304361934587433	29.914700461359487	21.952306996213196
26-27	21.879495135536896	26.337138789873553	29.96382229793786	21.81954377665169
28-29	21.86990141485495	26.423249723995433	29.962287481387524	21.744561379762096
30-31	21.877474324329164	26.385483886551743	29.927641807746802	21.809399981372295
32-33	21.88348425924736	26.426101036250373	29.87583854950517	21.814576154997102
34-35	21.90207915342645	26.51349026072003	29.83576058179258	21.74867000406094
36-37	21.90556641370795	26.512250738210508	29.752382775383087	21.82980007269845
38-39	21.972832838671287	26.56124894801813	29.610175316761612	21.855742896548975
40-41	22.062936118495077	26.736808286253044	29.347451606638998	21.85280398861288
42-43	22.210105644404116	26.89075229442668	28.88091495249061	22.018227108678595
44-45	22.359716191929135	27.097825016362908	28.41461881658846	22.1278399751195
46-47	22.49222098211487	27.335153899730223	27.98605099682837	22.186574121326537
48-49	22.618199360109013	27.408622965848288	27.60739719470642	22.36578047933628
50-51	22.63037174883482	27.494946586187787	27.45393186000935	22.420749804968047
52-53	22.680569511508722	27.57651549582546	27.34934408229721	22.393570910368606
54-55	22.69323827966683	27.571620659473105	27.255874568532327	22.479266492327735
56-57	22.698206950794088	27.61700997564048	27.22369903727624	22.461084036289186
58-59	22.71607824154111	27.723351027599623	27.147862578566066	22.412708152293195
60-61	22.703327104059795	27.730840828740245	27.08415753601231	22.48167453118765
62-63	22.729519092548603	27.793641210472952	27.04488537662485	22.43195432035359
64-65	22.749716499831987	27.939082097874845	26.94277173071928	22.368429671573892
66-67	22.778286757418705	27.983692510659935	26.823025016817837	22.414995715103522
68-69	22.793170797818714	28.070380963905805	26.72581838283706	22.410629855438422
70-71	22.84683220737325	28.17385771142704	26.636683610232346	22.34262647096736
72-73	22.8616708129441	28.23968983342158	26.513831178598586	22.384808175035733
74-75	22.921299973745057	28.291326266653527	26.460304304124172	22.327069455477243
76-77	22.707661512844453	28.515439421801442	26.333699074582633	22.443199990771472
78-79	22.7750006382507	28.56646710178936	26.223515997071495	22.43501626288845
80-81	22.782877734402145	28.652800856639555	26.119341836347726	22.444979572610574
82-83	22.799918028247276	28.80081312172429	26.00032936650425	22.398939483524188
84-85	22.81669650471217	28.852242885079594	25.853369404910232	22.477691205298004
86-87	22.817023772760724	28.948028528603242	25.715827599397162	22.51912009923888
88-89	22.883397552923626	29.028010288712387	25.608529532552478	22.48006262581151
90-91	22.897378689109292	29.097616073586714	25.445521122663177	22.559484114640817
92-93	22.890683427666964	29.185514493873765	25.295620778545057	22.628181299914214
94-95	22.963292104572364	29.28520000615199	25.151702928216963	22.599804961058684
96-97	22.970833267962572	29.346070311310317	24.974498885650465	22.70859753507665
98-99	22.98565869253236	29.427968917932308	24.805870671291704	22.780501718243627
100	23.605824216940217	29.277123677302576	24.009891819949353	23.107160285807858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4454.0
1	3075.5
2	1751.5
3	1928.5
4	2364.5
5	2962.0
6	3575.0
7	4353.0
8	5290.5
9	6307.0
10	7457.5
11	9161.0
12	11478.0
13	15198.0
14	23518.0
15	31716.5
16	40678.5
17	45479.0
18	46968.0
19	55520.5
20	67807.0
21	83678.5
22	103395.5
23	127940.0
24	157086.5
25	191593.5
26	231138.5
27	274565.5
28	322028.5
29	370149.0
30	415035.5
31	462848.5
32	517830.5
33	574958.0
34	636278.5
35	705583.0
36	781007.5
37	857844.0
38	930512.5
39	986948.0
40	1029788.5
41	1064416.0
42	1088355.5
43	1106336.5
44	1117241.5
45	1117182.5
46	1101710.0
47	1071458.5
48	1033853.5
49	992026.0
50	950907.5
51	915335.0
52	877901.5
53	835591.0
54	791212.0
55	744385.5
56	691561.5
57	634930.5
58	578299.5
59	523638.5
60	471379.5
61	420717.5
62	371705.0
63	324660.5
64	283026.0
65	244429.5
66	209297.5
67	179474.5
68	151070.5
69	124855.0
70	103224.0
71	85415.0
72	70789.0
73	59244.5
74	48892.0
75	38526.5
76	29736.5
77	23761.5
78	19606.0
79	15095.5
80	10469.0
81	6983.5
82	4663.0
83	3036.0
84	1940.0
85	1235.5
86	822.0
87	543.5
88	338.0
89	220.0
90	132.5
91	71.5
92	46.5
93	25.5
94	12.0
95	7.0
96	3.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07785062799712944
2	0.03151756265919864
3	8.406421137261707E-4
4	0.03405252221144267
5	0.05249777417192272
6	0.26043288181402746
7	0.27115269791541935
8	0.29722563665199614
9	0.28418590898094137
10-11	0.3012610446281584
12-13	0.32213210299820877
14-15	0.34187741776247466
16-17	0.3540455494435226
18-19	0.39362415314674665
20-21	0.4178757006369167
22-23	0.4162986820979886
24-25	0.41705786664255523
26-27	0.3862408390781284
28-29	0.3415597332427525
30-31	0.34977717281956416
32-33	0.3473806911348913
34-35	0.5565409206171551
36-37	0.590518501864971
38-39	0.6076636910216653
40-41	0.6020724434745564
42-43	0.6038563642391496
44-45	0.6080595748077805
46-47	0.6055539399804339
48-49	0.34005276821330155
50-51	0.10135113669964885
52-53	0.10869209283230412
54-55	0.030008968478364463
56-57	0.04462897299111379
58-59	0.030377156690965457
60-61	0.019114833178971242
62-63	0.025823678574948702
64-65	0.018435477052180904
66-67	0.022438302000679356
68-69	0.011474113191808952
70-71	0.01609112821177013
72-73	0.009960631556825209
74-75	0.01686008766463593
76-77	0.010993513533767828
78-79	0.004801109126261676
80-81	0.011314456356256308
82-83	0.015133187198454261
84-85	0.016842166999420836
86-87	0.006666487460014515
88-89	0.012373404755329971
90-91	0.004467133092707675
92-93	0.0038089559338988117
94-95	0.005112277040451014
96-97	0.0016275222318070629
98-99	0.0029666846687894507
100	0.0013554539508142905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.0690825E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.046426329276564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01189385678087	39.56147433765735
2	11.529565757276762	12.001453895893095
3	4.503979870158902	7.032481695023099
4	2.3809132592654167	4.956721061790211
5	1.404822688417915	3.6558000279219627
6	0.8855889661772716	2.7655044531699335
7	0.6287942860171805	2.2908546838424266
8	0.4548735968331123	1.8939636117366103
9	0.32099172180013635	1.503582480088059
>10	1.7404942896132616	16.37441683317207
>50	0.10519496310455449	3.664878688811271
>100	0.03171332477650746	2.9514482905482584
>500	8.329118923243654E-4	0.2736382382656643
>1k	2.2150848987576857E-4	0.18925306822691884
>5k	3.7578756627772946E-5	0.13245274372459032
>10k+	8.142063926626972E-5	0.7520758901284391
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32784	0.10682019789301851	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31169	0.101558038925314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	6.516605532761012E-6	0.0	3.258302766380506E-6	3.258302766380506E-6
2	0.0	9.774908299141519E-6	0.0	9.774908299141519E-6	3.258302766380506E-6
3	0.0	1.629151383190253E-5	0.0	1.3033211065522025E-5	3.258302766380506E-6
4	0.0	2.606642213104405E-5	0.0	1.3033211065522025E-5	3.258302766380506E-6
5	6.516605532761012E-6	2.606642213104405E-5	0.0	2.2808119364663545E-5	6.516605532761012E-6
6	6.516605532761012E-6	2.606642213104405E-5	0.0	2.2808119364663545E-5	6.516605532761012E-6
7	6.516605532761012E-6	3.258302766380506E-5	0.0	3.9099633196566075E-5	6.516605532761012E-6
8	6.516605532761012E-6	3.258302766380506E-5	0.0	3.9099633196566075E-5	6.516605532761012E-6
9	6.516605532761012E-6	3.584133043018557E-5	0.0	4.235793596294658E-5	9.774908299141519E-6
10-11	1.140405968233177E-5	4.7245390112517335E-5	0.0	5.0503692878897846E-5	9.774908299141519E-6
12-13	1.9549816598283038E-5	5.539114702846861E-5	0.0	5.864944979484911E-5	9.774908299141519E-6
14-15	6.190775256122962E-5	5.864944979484911E-5	0.0	6.516605532761012E-5	9.774908299141519E-6
16-17	1.41736170337552E-4	6.679520671080037E-5	0.0	7.494096362675165E-5	9.774908299141519E-6
18-19	2.785848865255333E-4	6.842435809399062E-5	0.0	7.98284177763224E-5	9.774908299141519E-6
20-21	4.626789928260319E-4	7.494096362675165E-5	0.0	8.960332607546391E-5	9.774908299141519E-6
22-23	7.771052097817507E-4	8.634502330908341E-5	0.0	9.611993160822494E-5	9.774908299141519E-6
24-25	0.0011892805097288848	9.449078022503468E-5	0.0	1.0263653714098594E-4	9.774908299141519E-6
26-27	0.0015884225986104968	9.937823437460544E-5	1.629151383190253E-6	1.0752399129055671E-4	9.774908299141519E-6
28-29	0.0020136311096231527	1.1566974820650797E-4	3.258302766380506E-6	1.1566974820650797E-4	1.3033211065522025E-5
30-31	0.0023606403542426766	1.2381550512245923E-4	3.258302766380506E-6	1.319612620384105E-4	1.4662362448712278E-5
32-33	0.002644112694917781	1.2707380788883974E-4	3.258302766380506E-6	1.41736170337552E-4	1.629151383190253E-5
34-35	0.0029666846687894507	1.35219564804791E-4	3.258302766380506E-6	1.5476938140307405E-4	1.629151383190253E-5
36-37	0.0033430186383063996	1.498819272535033E-4	3.258302766380506E-6	1.661734410854058E-4	1.629151383190253E-5
38-39	0.0037877769659173384	1.531402300198838E-4	3.258302766380506E-6	1.808358035341181E-4	1.629151383190253E-5
40-41	0.0044866829093059574	1.5802768416945455E-4	3.258302766380506E-6	1.9549816598283038E-4	1.629151383190253E-5
42-43	0.005399007683892499	1.7106089523497657E-4	3.258302766380506E-6	2.1504798258111343E-4	1.629151383190253E-5
44-45	0.006778898905454644	1.8246495491730833E-4	6.516605532761012E-6	2.394852533289672E-4	1.629151383190253E-5
46-47	0.008784384258161845	1.8735240906687912E-4	6.516605532761012E-6	2.541476157776795E-4	2.2808119364663545E-5
48-49	0.01157674972894994	1.9549816598283038E-4	6.516605532761012E-6	2.671808268432015E-4	2.2808119364663545E-5
50-51	0.015089200111108123	2.0364392289878164E-4	6.516605532761012E-6	2.6880997822639177E-4	2.606642213104405E-5
52-53	0.019836547241724522	2.085313770483524E-4	6.516605532761012E-6	2.753265837591528E-4	2.606642213104405E-5
54-55	0.02592957341485607	2.085313770483524E-4	6.516605532761012E-6	2.8347234067510407E-4	2.7695573514234304E-5
56-57	0.03382118271502965	2.1016052843154264E-4	6.516605532761012E-6	2.997638545070066E-4	2.9324724897424556E-5
58-59	0.04380136408845314	2.150479825811134E-4	9.774908299141519E-6	3.144262169557188E-4	3.095387628061481E-5
60-61	0.05628066368369049	2.2156458811387444E-4	9.774908299141519E-6	3.160553683389091E-4	3.258302766380506E-5
62-63	0.07258846902942492	2.3785610194577696E-4	9.774908299141519E-6	3.2094282248847983E-4	3.258302766380506E-5
64-65	0.09346278570224162	2.4437270747853796E-4	9.774908299141519E-6	3.225719738716701E-4	3.258302766380506E-5
66-67	0.11881075207329878	2.5740591854406E-4	9.774908299141519E-6	3.225719738716701E-4	3.258302766380506E-5
68-69	0.15058897895380785	2.6880997822639177E-4	9.774908299141519E-6	3.307177307876214E-4	3.258302766380506E-5
70-71	0.18953221361758765	2.8347234067510407E-4	1.3033211065522025E-5	3.4538009323633364E-4	3.421217904699532E-5
72-73	0.2369274856573585	2.965055517406261E-4	1.629151383190253E-5	3.535258501522849E-4	3.584133043018557E-5
74-75	0.2949822951973432	3.062804600397676E-4	1.629151383190253E-5	3.681882126009972E-4	3.584133043018557E-5
76-77	0.3654284301578729	3.144262169557188E-4	1.629151383190253E-5	3.7144651536737773E-4	3.584133043018557E-5
78-79	0.4503218795845338	3.160553683389091E-4	1.629151383190253E-5	3.7633396951694846E-4	3.9099633196566075E-5
80-81	0.5508014202941758	3.193136711052896E-4	1.629151383190253E-5	3.7796312090013874E-4	4.235793596294658E-5
82-83	0.667885271901293	3.307177307876214E-4	1.7920665215092786E-5	3.9099633196566075E-4	4.235793596294658E-5
84-85	0.8033329178997306	3.4863839600271415E-4	2.117896798147329E-5	4.0565869441437305E-4	4.398708734613683E-5
86-87	0.9607952865392181	3.5841330430185566E-4	2.2808119364663545E-5	4.089169971807535E-4	4.561623872932709E-5
88	1.0917432164172842	3.5841330430185566E-4	2.2808119364663545E-5	4.170627540967048E-4	4.8874541495707594E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20910	0.0	46.48025	1
TCTCGAG	25270	0.0	38.187096	2
TATCTCG	8915	0.0	31.96655	2
CTCGAGG	32315	0.0	29.809488	3
GATCCGG	35495	0.0	28.150072	1
TCGAGGG	38100	0.0	25.223	4
ATCCGGC	40160	0.0	24.81314	2
TCCGGCG	46250	0.0	21.519186	3
ATATCTC	14505	0.0	20.594353	1
CGAGGGC	51420	0.0	18.79041	5
GCGCCGT	56765	0.0	17.452679	9
CGGCGCG	58830	0.0	16.943125	5
AGGGCGC	57000	0.0	16.83938	7
GAGGGCG	58365	0.0	16.427101	6
CCGGCGC	61605	0.0	16.22513	4
TATGGAT	21470	0.0	15.682868	2
ATATGGA	22000	0.0	15.524089	1
GGGCGCG	63490	0.0	15.140292	8
CGTCGAC	54770	0.0	14.550888	12-13
GGATCCG	23915	0.0	14.512108	2
>>END_MODULE