FastQCFastQC Report
Wed 25 May 2016
SRR445718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR445718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32943665
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA231550.045.2424661
TCTCGAG278900.037.482582
TATCTCG97150.034.706822
CTCGAGG355650.029.1870213
GATCCGG409000.025.8549561
TCGAGGG432650.023.972034
ATCCGGC464250.022.7406542
ATATCTC156100.021.9079781
TCCGGCG530600.019.979883
ATATGGA236000.018.9357471
TATGGAT235150.018.6184372
CGAGGGC571500.018.1568835
GCGCCGT642000.016.4051659
AGGGCGC641650.016.058197
GAGGGCG652200.015.8899696
CGGCGCG667750.015.8425045
GGATCCG283350.015.6836482
CCGGCGC699150.015.2244664
GGGCGCG712900.014.5918038
CGTCGAC617650.014.13348512-13
Produced by FastQC (version 0.11.5)