##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR445718_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32943665
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782310863105245	33.0	31.0	34.0	30.0	34.0
2	32.03416562789841	34.0	31.0	34.0	30.0	34.0
3	32.11844632344337	34.0	31.0	34.0	30.0	34.0
4	35.49483480359577	37.0	35.0	37.0	33.0	37.0
5	35.27748530711443	37.0	35.0	37.0	33.0	37.0
6	35.15521773306036	37.0	35.0	37.0	33.0	37.0
7	35.11097128992782	37.0	35.0	37.0	32.0	37.0
8	35.07638470097362	37.0	35.0	37.0	32.0	37.0
9	36.73787099887034	39.0	37.0	39.0	33.0	39.0
10-11	36.67481606858253	39.0	37.0	39.0	33.0	39.0
12-13	36.60932077532964	39.0	37.0	39.0	33.0	39.0
14-15	37.873022673706764	40.0	38.0	41.0	33.0	41.0
16-17	37.76935600516822	40.0	38.0	41.0	32.0	41.0
18-19	37.67176100473338	40.0	38.0	41.0	32.0	41.0
20-21	37.56245592286105	40.0	38.0	41.0	32.0	41.0
22-23	37.41654125307552	40.0	38.0	41.0	32.0	41.0
24-25	37.276153123825175	40.0	38.0	41.0	31.5	41.0
26-27	36.9929681928225	40.0	37.0	41.0	31.0	41.0
28-29	36.85683431093656	40.0	37.0	41.0	30.5	41.0
30-31	36.67535656703649	40.0	36.5	41.0	30.0	41.0
32-33	36.67013185994941	40.0	36.5	41.0	30.0	41.0
34-35	36.49602762777001	40.0	36.0	41.0	30.0	41.0
36-37	36.35818604274904	40.0	36.0	41.0	30.0	41.0
38-39	36.11927830434167	40.0	36.0	41.0	29.0	41.0
40-41	35.798483562772994	40.0	35.0	41.0	27.0	41.0
42-43	35.4247791039643	40.0	35.0	41.0	25.0	41.0
44-45	35.00713225137518	39.5	35.0	41.0	23.5	41.0
46-47	34.61990077606727	39.0	35.0	41.0	20.0	41.0
48-49	34.161432584990166	39.0	34.0	41.0	15.0	41.0
50-51	33.81218244843129	39.0	34.0	41.0	9.0	41.0
52-53	33.53826813136911	38.5	33.0	40.5	7.5	41.0
54-55	33.084819463772476	38.0	33.0	40.0	2.0	41.0
56-57	32.78255814888841	38.0	32.5	40.0	2.0	41.0
58-59	32.371354598828034	38.0	32.0	40.0	2.0	41.0
60-61	31.903407180105795	37.0	31.0	40.0	2.0	41.0
62-63	31.497318057963497	36.5	31.0	40.0	2.0	41.0
64-65	30.98814662849443	36.0	30.0	39.5	2.0	41.0
66-67	30.464408346794443	35.5	29.0	39.0	2.0	41.0
68-69	29.88746378097276	35.0	28.5	39.0	2.0	40.0
70-71	29.350980013911627	35.0	28.0	38.0	2.0	40.0
72-73	28.812276806481613	34.5	26.5	37.5	2.0	40.0
74-75	28.198697321624657	34.0	26.0	37.0	2.0	39.0
76-77	27.425911537165035	33.5	25.5	36.0	2.0	39.0
78-79	27.48233482825909	34.0	25.5	36.0	2.0	39.0
80-81	27.045210148901162	34.0	24.5	35.5	2.0	37.5
82-83	26.506165358347346	34.0	22.5	35.0	2.0	37.0
84-85	25.89786186509607	33.0	19.5	35.0	2.0	36.5
86-87	25.30796567412885	33.0	16.5	35.0	2.0	36.0
88-89	24.791233079258184	32.5	8.5	35.0	2.0	36.0
90-91	24.1699928195603	32.0	2.0	35.0	2.0	35.0
92-93	23.450610716810047	31.5	2.0	35.0	2.0	35.0
94-95	22.81846907440323	31.0	2.0	34.0	2.0	35.0
96-97	22.119780904765754	31.0	2.0	34.0	2.0	35.0
98-99	21.373683802333467	30.0	2.0	34.0	2.0	35.0
100	20.238112092264174	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09344987785397052
1101	2	0.12260416452474487
1101	3	0.09298375333834485
1101	4	0.11069145426768046
1101	5	0.12292526930921355
1101	6	0.2022964601960453
1101	7	0.19677680301017375
1101	8	0.19680743148040136
1101	9	0.2274648739612246
1101	10-11	0.18351103329990792
1101	12-13	0.1574068584474091
1101	14-15	0.15953944926150143
1101	16-17	0.10560212555488846
1101	18-19	0.1382253416024568
1101	20-21	0.11786375155405437
1101	22-23	0.12647637957163482
1101	24-25	0.14580715429304547
1101	26-27	0.12755668400415487
1101	28-29	0.010106970410809879
1101	30-31	-0.02322400483542708
1101	32-33	-0.03643410699297789
1101	34-35	-0.09174768728142624
1101	36-37	-0.045457195475563594
1101	38-39	-0.05958412722755213
1101	40-41	-0.11068611124484562
1101	42-43	-0.15964730696447305
1101	44-45	-0.21096652815496952
1101	46-47	-0.2579986504530538
1101	48-49	-0.25237519742358927
1101	50-51	-0.24180465765061854
1101	52-53	-0.25793732992699603
1101	54-55	-0.31372264169936415
1101	56-57	-0.28564053566425684
1101	58-59	-0.418341553198708
1101	60-61	-0.4255883311736497
1101	62-63	-0.46886561795237824
1101	64-65	-0.4844605023463906
1101	66-67	-0.5855616180505585
1101	68-69	-0.5457157550932976
1101	70-71	-0.5312566251455735
1101	72-73	-0.6032470986315488
1101	74-75	-0.6501614930815691
1101	76-77	-0.5128242155104665
1101	78-79	-0.4464744293617926
1101	80-81	-0.48718159517643755
1101	82-83	-0.4806816283374502
1101	84-85	-0.5397792177219252
1101	86-87	-0.6187784955730145
1101	88-89	-0.7147037457053251
1101	90-91	-0.840349591568657
1101	92-93	-0.7723897276561225
1101	94-95	-0.8461639341973779
1101	96-97	-0.9197749723530819
1101	98-99	-0.8998580451584246
1101	100	-0.7130476959203413
1102	1	0.17118102004604197
1102	2	0.17480977734820868
1102	3	0.13225099199340207
1102	4	0.1596866353487414
1102	5	0.1938872901262343
1102	6	0.24991174973937547
1102	7	0.28023456726987916
1102	8	0.24541808428156742
1102	9	0.2553271043383347
1102	10-11	0.2814422501974718
1102	12-13	0.2409825042889011
1102	14-15	0.2993584800988174
1102	16-17	0.24828931724774606
1102	18-19	0.27558091626845993
1102	20-21	0.30319025549587764
1102	22-23	0.2530371293074154
1102	24-25	0.22528825451774281
1102	26-27	0.2041900655408
1102	28-29	0.1757413041542364
1102	30-31	0.18907418661594733
1102	32-33	0.1288505180223538
1102	34-35	0.03647047940624759
1102	36-37	0.10130219050331846
1102	38-39	0.05842689129662659
1102	40-41	-0.0017834342542641934
1102	42-43	0.04857812284759433
1102	44-45	-0.043581142882651136
1102	46-47	-0.0494915014904862
1102	48-49	0.004242236492387974
1102	50-51	0.07137679958439946
1102	52-53	-0.05423138387119053
1102	54-55	-0.02815169518402172
1102	56-57	-0.0017859071354990874
1102	58-59	-0.07492524076338469
1102	60-61	-0.031195302153371074
1102	62-63	-0.03428489126518386
1102	64-65	-0.058965558387324535
1102	66-67	-0.1385565590673572
1102	68-69	-0.09981176528471991
1102	70-71	-0.154912706522218
1102	72-73	-0.24150647653977586
1102	74-75	-0.3609312321359752
1102	76-77	-0.24860214517482504
1102	78-79	-0.1943434418412764
1102	80-81	-0.18772088881777904
1102	82-83	-0.2556707714950335
1102	84-85	-0.3927968933376995
1102	86-87	-0.5092910947877378
1102	88-89	-0.5768125127532642
1102	90-91	-0.6500107616679891
1102	92-93	-0.5391864619030748
1102	94-95	-0.5532934523106441
1102	96-97	-0.6530878100569097
1102	98-99	-0.7084087062007356
1102	100	-0.6959449768930206
1103	1	0.164042794824109
1103	2	0.15658043188937398
1103	3	0.13650820315959322
1103	4	0.10280600694203201
1103	5	0.12347254937211716
1103	6	0.18561858261178799
1103	7	0.22452237562667676
1103	8	0.18824060979353163
1103	9	0.16781287933669375
1103	10-11	0.1900994133907048
1103	12-13	0.20233870259247055
1103	14-15	0.21600964303586068
1103	16-17	0.19891122047757648
1103	18-19	0.21657787390577
1103	20-21	0.27246475025092565
1103	22-23	0.2739577472871346
1103	24-25	0.23993851308262748
1103	26-27	0.22540289851042417
1103	28-29	0.14766841703955436
1103	30-31	0.10679985057068109
1103	32-33	0.20686655585046054
1103	34-35	0.15310487908868708
1103	36-37	0.17571270229404234
1103	38-39	0.21777209143303367
1103	40-41	0.08533261371565004
1103	42-43	0.059090371583629064
1103	44-45	0.09747557734580425
1103	46-47	0.051358801189586245
1103	48-49	0.126184336179179
1103	50-51	0.08748909522292081
1103	52-53	-0.03525276559818025
1103	54-55	0.13513544153771306
1103	56-57	-0.02206858409768131
1103	58-59	-0.11427923751697477
1103	60-61	-0.08374832398892806
1103	62-63	-0.04507214283988148
1103	64-65	-0.07837059192983631
1103	66-67	-0.21691452749642437
1103	68-69	-0.1365405477297621
1103	70-71	-0.21747532374740786
1103	72-73	-0.2444265335024305
1103	74-75	-0.3413287464434873
1103	76-77	-0.2753051663201411
1103	78-79	-0.1653616323687075
1103	80-81	-0.20639753395670013
1103	82-83	-0.26112396985528363
1103	84-85	-0.34653133999363206
1103	86-87	-0.4498287017019571
1103	88-89	-0.6904575334968719
1103	90-91	-0.8047190243555953
1103	92-93	-0.784955007266575
1103	94-95	-0.6470808590183914
1103	96-97	-0.6018529984388827
1103	98-99	-0.6311220424553738
1103	100	-0.6190064168722564
1104	1	0.1342165553643575
1104	2	0.12222469401740454
1104	3	0.10544842870200455
1104	4	0.10489579518573322
1104	5	0.07178278563302598
1104	6	0.14569458540117353
1104	7	0.16168513688312913
1104	8	0.1314596342786274
1104	9	0.15534680691460778
1104	10-11	0.16030977495051957
1104	12-13	0.1765834002799025
1104	14-15	0.14352405254300038
1104	16-17	0.14735686567286876
1104	18-19	0.16030332612758968
1104	20-21	0.13901520284233015
1104	22-23	0.1966262362101432
1104	24-25	0.2030283864178628
1104	26-27	0.1768832010650172
1104	28-29	0.1298669402565622
1104	30-31	0.11957168982348776
1104	32-33	0.1991076836064991
1104	34-35	0.08674026036226223
1104	36-37	0.07530078743538127
1104	38-39	0.00597570353532717
1104	40-41	0.04039844085530575
1104	42-43	0.0032017164479825055
1104	44-45	0.054392834544799484
1104	46-47	0.028270450940347303
1104	48-49	0.16634767247846582
1104	50-51	0.16060466951911678
1104	52-53	0.15517987636194874
1104	54-55	0.21107514423637497
1104	56-57	0.13884701065084926
1104	58-59	0.145778826947506
1104	60-61	0.1494242721989565
1104	62-63	0.14303677763969702
1104	64-65	0.17646895809787466
1104	66-67	0.13988089064894638
1104	68-69	0.14945549592485108
1104	70-71	0.1083804105207129
1104	72-73	0.08567016821501383
1104	74-75	0.05580963463724231
1104	76-77	0.06632772617676252
1104	78-79	0.084667477575195
1104	80-81	0.09044072749737353
1104	82-83	0.09144562217186802
1104	84-85	0.06497426338120604
1104	86-87	-0.00829947953787169
1104	88-89	-0.17664479135568456
1104	90-91	-0.28064882707354144
1104	92-93	-0.21321494334251057
1104	94-95	-0.17571910361067822
1104	96-97	-0.1950327530213798
1104	98-99	-0.24227816059328688
1104	100	-0.16867078285891068
1105	1	-0.02537527901405312
1105	2	-0.021224610671694677
1105	3	-0.011994329958589844
1105	4	0.0030166261565582886
1105	5	0.037551865455327516
1105	6	0.0741868218097963
1105	7	0.06613006897168816
1105	8	0.05561106702254648
1105	9	0.06948637810439351
1105	10-11	0.038275225093286736
1105	12-13	0.0594372855550418
1105	14-15	0.017311352526206747
1105	16-17	0.06710814990631064
1105	18-19	0.08341510797064444
1105	20-21	0.10422033784465157
1105	22-23	0.1610380607055717
1105	24-25	0.11033536485933126
1105	26-27	0.07847317460993253
1105	28-29	0.0699496425863515
1105	30-31	0.0014891749589622805
1105	32-33	0.010087179595132056
1105	34-35	-0.07595155720947844
1105	36-37	-0.07506862716649465
1105	38-39	0.01939126740337116
1105	40-41	-0.0865747289775669
1105	42-43	-0.02924520753094839
1105	44-45	0.0025497894967685397
1105	46-47	-0.059349423456772854
1105	48-49	4.0198791811718593E-4
1105	50-51	0.04540960751580059
1105	52-53	0.043548780004051935
1105	54-55	0.052187991623341645
1105	56-57	0.05807675947900748
1105	58-59	-0.020227399891368236
1105	60-61	-0.017649722089529973
1105	62-63	-0.03435813807860555
1105	64-65	-0.004367019294008401
1105	66-67	-0.09855478261359707
1105	68-69	-0.007700634263631656
1105	70-71	-0.0033600983755732727
1105	72-73	0.0024974510437303366
1105	74-75	0.031347930557778625
1105	76-77	-0.0037008256239907666
1105	78-79	0.012481267100991289
1105	80-81	-0.05771998043592674
1105	82-83	-0.09891184552808596
1105	84-85	-0.06697949321369379
1105	86-87	-0.12656037579668933
1105	88-89	-0.2667303928260729
1105	90-91	-0.25043309388939505
1105	92-93	-0.16210743997556776
1105	94-95	-0.09811553257758732
1105	96-97	-0.14020001096641366
1105	98-99	-0.21382403215746137
1105	100	-0.17675277792173816
1106	1	-0.004030901353413441
1106	2	-0.005835247709377711
1106	3	-0.02666068054070081
1106	4	0.0020307854550409843
1106	5	-0.0677046169962523
1106	6	-0.03798244715554233
1106	7	0.003691232073741446
1106	8	-0.016588050612867278
1106	9	-0.04249893224866952
1106	10-11	-0.03127464511190681
1106	12-13	-0.010058950469719719
1106	14-15	-0.08116631841930655
1106	16-17	-0.07309724380613858
1106	18-19	-0.0920491386523139
1106	20-21	0.045867187716311264
1106	22-23	0.06648389278266364
1106	24-25	0.012862647889171797
1106	26-27	0.06741294627305905
1106	28-29	0.03332237876126243
1106	30-31	0.07763885080183996
1106	32-33	0.11449630075302508
1106	34-35	0.01978672073511234
1106	36-37	0.004846704027663407
1106	38-39	0.07759302626108422
1106	40-41	0.09080953908239309
1106	42-43	0.027563526870643784
1106	44-45	0.06628659727674346
1106	46-47	0.07754249291379978
1106	48-49	0.11280948480377617
1106	50-51	0.12273032695942732
1106	52-53	0.08321271628776827
1106	54-55	0.20598198818338176
1106	56-57	0.2144688832052779
1106	58-59	0.17470914695541495
1106	60-61	0.22998582576947513
1106	62-63	0.24191479544716543
1106	64-65	0.09476797994739528
1106	66-67	0.11161046900626914
1106	68-69	0.24986460163961155
1106	70-71	0.22771018528916187
1106	72-73	0.2666871305301264
1106	74-75	0.27211491367994256
1106	76-77	0.19721156403950957
1106	78-79	0.2613869641238509
1106	80-81	0.3889535281132339
1106	82-83	0.35888513192175253
1106	84-85	0.4661577105850654
1106	86-87	0.2853030923007367
1106	88-89	0.23818376660456764
1106	90-91	0.22799980233647332
1106	92-93	0.33612494043056174
1106	94-95	0.36467474401937494
1106	96-97	0.38395350765758707
1106	98-99	0.3639756204373725
1106	100	0.305214441719194
1107	1	-0.10628686741697635
1107	2	-0.06376414146072662
1107	3	-0.11348444906792565
1107	4	-0.09208690014700238
1107	5	-0.0801648145994136
1107	6	-0.1203956644437767
1107	7	-0.15609281581468082
1107	8	-0.10461357290748197
1107	9	-0.09354256291482699
1107	10-11	-0.1190876588320009
1107	12-13	-0.08191024079406617
1107	14-15	-0.05426847165028903
1107	16-17	-0.11988961550282795
1107	18-19	-0.15268664592629477
1107	20-21	-0.11333571000361076
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2207	70-71	0.5494614385618064
2207	72-73	0.5727726508346933
2207	74-75	0.6265533872834688
2207	76-77	0.5621299964019073
2207	78-79	0.45625973539304
2207	80-81	0.47932803403970325
2207	82-83	0.539765251311831
2207	84-85	0.5867464890154572
2207	86-87	0.6637858994312786
2207	88-89	0.6746071829081721
2207	90-91	0.6615427797743259
2207	92-93	0.6682655851325521
2207	94-95	0.5682014323203433
2207	96-97	0.611337736966501
2207	98-99	0.6111070354795274
2207	100	0.6079928104322967
2208	1	-0.32926266616021493
2208	2	-0.38473765735946586
2208	3	-0.30201094619559754
2208	4	-0.2661354429728533
2208	5	-0.24254022147143672
2208	6	-0.41237232244080246
2208	7	-0.49052675026585746
2208	8	-0.47530090335276753
2208	9	-0.47090069161419734
2208	10-11	-0.45642674061051025
2208	12-13	-0.42776421212290217
2208	14-15	-0.4569712913682906
2208	16-17	-0.4268426780313064
2208	18-19	-0.5900627755456256
2208	20-21	-0.6978509467716947
2208	22-23	-0.6699543010766078
2208	24-25	-0.32840741535142115
2208	26-27	-0.37736794153261854
2208	28-29	-0.17109939837468602
2208	30-31	-0.17683060271679096
2208	32-33	-0.4052202017016029
2208	34-35	-0.22075490636225226
2208	36-37	-0.1568513480549214
2208	38-39	-0.1699830871339998
2208	40-41	-0.22527291392786708
2208	42-43	-0.2945600910408359
2208	44-45	-0.09959115129532137
2208	46-47	-0.01103451626918428
2208	48-49	-0.1798037064680571
2208	50-51	-0.34907780185906745
2208	52-53	-0.17803329616674546
2208	54-55	-1.0456967614937582
2208	56-57	-0.29396671233693183
2208	58-59	-0.02521154571076778
2208	60-61	-0.11490011327159166
2208	62-63	-0.08451740233538274
2208	64-65	0.14281811303148118
2208	66-67	0.34799803340896673
2208	68-69	-0.025398338436414036
2208	70-71	-0.060726790459213476
2208	72-73	0.37266665866909676
2208	74-75	0.6195474011471092
2208	76-77	0.6603485772941333
2208	78-79	0.6364400911065715
2208	80-81	0.44891654339633646
2208	82-83	0.5128123819066914
2208	84-85	0.5378999922732781
2208	86-87	0.9968249135661402
2208	88-89	1.0958718275402397
2208	90-91	1.2655153020956043
2208	92-93	1.0034785274064753
2208	94-95	1.411150066955983
2208	96-97	1.1666030620150813
2208	98-99	1.2769606653701722
2208	100	1.2430677509514467
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	412619.0
3	205407.0
4	92809.0
5	89826.0
6	82717.0
7	90999.0
8	92114.0
9	104220.0
10	119150.0
11	130193.0
12	151523.0
13	202892.0
14	271132.0
15	351979.0
16	443102.0
17	436473.0
18	382319.0
19	329376.0
20	319073.0
21	328701.0
22	349036.0
23	373408.0
24	406104.0
25	444642.0
26	493656.0
27	555660.0
28	630907.0
29	721795.0
30	832031.0
31	969047.0
32	1143730.0
33	1392921.0
34	1775327.0
35	2385016.0
36	3461490.0
37	5306077.0
38	5813367.0
39	1252222.0
40	605.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.267927304606108	27.990105134840853	28.264682023341052	22.477285537211987
2	23.639432100545278	27.163698829332418	27.27593590766074	21.920933162461562
3	23.557629131185752	26.149910314403634	26.975122222445837	23.317338331964773
4	24.12788636576811	25.83001438713568	26.883954592145916	23.1581446549503
5	24.664795166453963	26.07019395277087	26.385914706409192	22.87909617436598
6	24.66324810463977	26.02737182903213	25.763106372993743	23.546273693334353
7	24.489863439554455	26.470536402305335	25.971577191710978	23.068022966429233
8	24.078123691328553	26.147507820708093	26.240737365260973	23.533631122702374
9	23.96994853441312	25.960912772003773	26.36511476803956	23.704023925543545
10-11	23.764932398126483	26.12554193481522	26.368503008553436	23.74102265850486
12-13	23.43020294972757	26.209907130378614	26.591572432296317	23.768317487597503
14-15	23.16477024582225	26.479974376734646	27.071776043571855	23.28347933387125
16-17	22.910594970028715	26.397230280500906	27.358246936101228	23.333927813369154
18-19	22.47335454154562	26.40327958263038	28.132748604334125	22.990617271489878
20-21	22.16878684266324	26.10835856520174	29.0073222307016	22.715532361433425
22-23	22.101154289987264	26.073320577048065	29.427005970768167	22.39851916219651
24-25	22.040957326203092	25.951146265394083	29.836021670116864	22.17187473828596
26-27	22.104371727844814	25.956263234450233	29.921714721112686	22.01765031659227
28-29	22.080049506517163	26.060902129383933	29.90419671414716	21.95485164995174
30-31	22.089160893612984	26.01795967731974	29.877069219209538	22.01581020985774
32-33	22.101787260184157	26.058775974273168	29.830161142075667	22.00927562346701
34-35	22.114660864345282	26.146020896207865	29.785377477348536	21.95394076209832
36-37	22.126320026075366	26.157674893020726	29.691098245541873	22.02490683536203
38-39	22.204095971425446	26.179375426727635	29.56140908497395	22.05511951687297
40-41	22.272325500248797	26.37658850113508	29.285632792181083	22.065453206435045
42-43	22.432991920371332	26.55729651677423	28.786842087020226	22.22286947583421
44-45	22.606834792975324	26.76088663180172	28.281348138586164	22.35093043663679
46-47	22.75115282222574	27.023653059248474	27.800805133659672	22.42438898486611
48-49	22.88201375686312	27.11105722210948	27.406326412444386	22.600602608583014
50-51	22.911295181734147	27.21243855204551	27.220733245425787	22.655533020794554
52-53	22.94246341018234	27.283005629137268	27.126975955286554	22.647555005393833
54-55	22.964452324353317	27.27387406267726	27.024243189229118	22.737430423740307
56-57	22.96532846562932	27.332499080632022	26.990914340930267	22.71125811280839
58-59	22.980663504101383	27.42246374781825	26.943985884370896	22.65288686370947
60-61	22.97414390893946	27.439689806573586	26.877357655362065	22.70880862912489
62-63	22.972350046419926	27.507836674119783	26.83964143054322	22.680171848917066
64-65	22.999917753902494	27.670047905239866	26.72827999425461	22.601754346603027
66-67	23.02554594235474	27.7213683974952	26.610141810980814	22.642943849169246
68-69	23.059669047658875	27.81969585890477	26.48621312220383	22.634421971232523
70-71	23.098148468892628	27.944304545315855	26.400758810039886	22.556788175751628
72-73	23.112092665380114	28.010887387700745	26.271429907387105	22.605590039532032
74-75	23.182551740135526	28.06361689201107	26.21988792718204	22.533943440671365
76-77	22.954171286861307	28.31780268417984	26.065587483916442	22.662438545042416
78-79	23.020646918724704	28.36688603399618	25.954808716176338	22.657658331102777
80-81	23.015587962052496	28.478792705557016	25.85322923467535	22.652390097715138
82-83	23.032991365872903	28.628553717754997	25.733904750183136	22.604550166188968
84-85	23.03567782397733	28.701355854857603	25.60057601292993	22.662390308235135
86-87	23.01130398778089	28.79014389030936	25.467362742695833	22.731189379213916
88-89	23.06133150680074	28.908053772530145	25.352925793612098	22.677688927057012
90-91	23.0883891661507	28.976203976350824	25.17618424791027	22.7592226095882
92-93	23.071093561781083	29.074982192804377	25.021987213667817	22.831937031746723
94-95	23.152190871363878	29.18008652080625	24.866384553818403	22.801338054011474
96-97	23.149537435773183	29.256123773491527	24.69297086388882	22.901367926846472
98-99	23.16265411800704	29.333620093923706	24.516614381428113	22.98711140664114
100	23.79851149948305	29.192935602530657	23.70016045790302	23.30839244008327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2745.0
1	1892.0
2	1063.5
3	1225.5
4	1568.5
5	1944.0
6	2424.5
7	2980.0
8	3666.5
9	4532.5
10	5567.5
11	7158.5
12	9165.0
13	12141.5
14	18590.5
15	25520.5
16	33926.0
17	39928.5
18	43680.5
19	52754.0
20	67040.5
21	84567.5
22	104309.5
23	128816.0
24	158068.5
25	191952.5
26	230493.5
27	272392.0
28	318823.0
29	368635.0
30	414161.0
31	460450.5
32	516318.0
33	577860.0
34	643545.5
35	719653.5
36	803699.5
37	886191.0
38	967555.5
39	1035612.5
40	1089442.0
41	1133994.5
42	1160687.5
43	1181612.5
44	1198785.5
45	1202121.0
46	1196024.5
47	1176019.0
48	1140998.0
49	1097549.5
50	1054826.0
51	1020250.0
52	981086.5
53	934048.0
54	885133.5
55	833251.0
56	775538.5
57	713235.0
58	648117.5
59	584956.0
60	525585.5
61	469251.0
62	415377.0
63	364216.0
64	317420.0
65	273775.5
66	235352.5
67	202022.5
68	170172.0
69	140737.0
70	116460.0
71	96856.0
72	80527.5
73	67132.0
74	55752.0
75	44635.5
76	34837.5
77	27890.0
78	22841.5
79	17854.5
80	12578.0
81	8234.0
82	5404.5
83	3519.5
84	2288.5
85	1497.0
86	979.5
87	622.0
88	376.0
89	235.0
90	135.0
91	70.0
92	43.0
93	30.0
94	18.0
95	7.0
96	4.5
97	4.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07151602591879198
2	0.02976900111144282
3	7.528002728293892E-4
4	0.03269824410854105
5	0.050944544269740474
6	0.2604294330943445
7	0.27099595627869577
8	0.29703434636067355
9	0.2845858224942489
10-11	0.3014509769936041
12-13	0.3221378070715568
14-15	0.3416878480278378
16-17	0.35411512349946495
18-19	0.39306343116347253
20-21	0.416677986496038
22-23	0.4153484440787022
24-25	0.41617864921829434
26-27	0.3857691607779523
28-29	0.3412856462691689
30-31	0.3492887023954378
32-33	0.3468997757231929
34-35	0.5554451819492457
36-37	0.5888248924337958
38-39	0.6057750405123413
40-41	0.6005980208941537
42-43	0.6024587731814296
44-45	0.6064625778582924
46-47	0.6038050107661063
48-49	0.33616174763797535
50-51	0.09427002126205448
52-53	0.0999828039776388
54-55	0.029863101145546495
56-57	0.04490544691976439
58-59	0.030225841599591304
60-61	0.019065881103392714
62-63	0.025668060915505303
64-65	0.018006496848483617
66-67	0.022335098417252605
68-69	0.011401281551399944
70-71	0.015936296098202796
72-73	0.009605792191002428
74-75	0.016775607692708142
76-77	0.010880695878858652
78-79	0.004803654966743985
80-81	0.011021845930014162
82-83	0.01504386351670344
84-85	0.016742217358026196
86-87	0.0065217394603788005
88-89	0.0122056850687378
90-91	0.004175309577729132
92-93	0.003713915862124023
94-95	0.0049979260048935055
96-97	0.0013841811468153285
98-99	0.002944420421953659
100	0.0013507908121333798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.2943665E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.2375930935945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02366981531684	36.93984926754759
2	11.53848502219576	11.36254460878819
3	4.645619016159101	6.8621729635651985
4	2.509677542197281	4.942819264753682
5	1.4962229505248683	3.683520840762042
6	0.9768275874668207	2.8857983564573346
7	0.6868945375025021	2.3674723615032685
8	0.5235968505158325	2.062451893662895
9	0.401007235417148	1.777016797655111
>10	2.0416329232993835	18.208993530049042
>50	0.1135674208172994	3.7519593065015573
>100	0.040931626550843876	3.6761197954826783
>500	0.0014687312560446092	0.4520270484051901
>1k	2.8777815918103677E-4	0.24280605684968318
>5k	3.698754047690452E-5	0.13022329466733767
>10k+	7.397508054571595E-5	0.654224613349148
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.2141939884344987E-5	0.0	3.035484971086247E-6	0.0
2	0.0	1.5177424855431234E-5	0.0	3.035484971086247E-6	0.0
3	0.0	2.731936473977622E-5	0.0	1.2141939884344987E-5	0.0
4	0.0	2.731936473977622E-5	0.0	1.821290982651748E-5	0.0
5	0.0	3.339033468194872E-5	0.0	2.4283879768689974E-5	0.0
6	0.0	4.2496789595207456E-5	0.0	3.339033468194872E-5	0.0
7	0.0	4.2496789595207456E-5	0.0	3.642581965303496E-5	0.0
8	0.0	4.2496789595207456E-5	0.0	4.2496789595207456E-5	0.0
9	6.070969942172494E-6	4.55322745662937E-5	0.0	5.767421445063869E-5	0.0
10-11	1.365968236988811E-5	5.0085502022923075E-5	0.0	6.678066936389743E-5	0.0
12-13	3.642581965303496E-5	5.919195693618181E-5	0.0	7.588712427715618E-5	0.0
14-15	7.740486676269929E-5	6.222744190726806E-5	0.0	8.044035173378554E-5	0.0
16-17	1.684694158952867E-4	6.981615433498367E-5	0.0	8.802906416150117E-5	3.035484971086247E-6
18-19	3.5363399913154774E-4	6.981615433498367E-5	3.035484971086247E-6	9.258229161813052E-5	3.035484971086247E-6
20-21	5.964727968184475E-4	7.133389682052681E-5	3.035484971086247E-6	9.71355190747599E-5	3.035484971086247E-6
22-23	9.470713109789089E-4	7.740486676269929E-5	3.035484971086247E-6	1.0472423150247552E-4	6.070969942172494E-6
24-25	0.0013735569494165267	8.954680664704427E-5	3.035484971086247E-6	1.0927745895910488E-4	6.070969942172494E-6
26-27	0.001882000682073473	1.0320648901693239E-4	3.035484971086247E-6	1.1686617138682051E-4	6.070969942172494E-6
28-29	0.002411692809528023	1.092774589591049E-4	3.035484971086247E-6	1.2445488381453612E-4	6.070969942172494E-6
30-31	0.0028503203878499856	1.1534842890127738E-4	3.035484971086247E-6	1.2900811127116547E-4	9.10645491325874E-6
32-33	0.003196365674553818	1.365968236988811E-4	6.070969942172494E-6	1.3963230866996735E-4	9.10645491325874E-6
34-35	0.003556070643627538	1.426677936410536E-4	6.070969942172494E-6	1.563274760109417E-4	9.10645491325874E-6
36-37	0.003937024007498862	1.5177424855431233E-4	6.070969942172494E-6	1.6695167340974358E-4	1.2141939884344987E-5
38-39	0.004463680649982326	1.563274760109417E-4	6.070969942172494E-6	1.821290982651748E-4	1.2141939884344987E-5
40-41	0.005219516407782801	1.6239844595311418E-4	6.070969942172494E-6	1.8668232572180416E-4	1.2141939884344987E-5
42-43	0.006145339323964107	1.6998715838082982E-4	9.10645491325874E-6	1.9427103814951977E-4	1.2141939884344987E-5
44-45	0.007335249432629915	1.821290982651748E-4	9.10645491325874E-6	2.018597505772354E-4	1.2141939884344987E-5
46-47	0.009039674243894843	1.957887806350629E-4	9.10645491325874E-6	2.0944846300495105E-4	1.2141939884344987E-5
48-49	0.011616800984347066	2.0793072051940791E-4	1.2141939884344987E-5	2.21590402889296E-4	1.2141939884344987E-5
50-51	0.014913337662946731	2.2310814537483914E-4	1.2141939884344987E-5	2.2917911531701162E-4	1.2141939884344987E-5
52-53	0.019138732742698786	2.3525008525918412E-4	1.2141939884344987E-5	2.3828557023027036E-4	1.365968236988811E-5
54-55	0.02471491863458422	2.473920251435291E-4	1.2141939884344987E-5	2.473920251435291E-4	1.5177424855431234E-5
56-57	0.031898393818659825	2.5801622254233094E-4	1.2141939884344987E-5	2.5194525260015846E-4	1.821290982651748E-5
58-59	0.04105038100648486	2.6256944999896034E-4	1.5177424855431234E-5	2.534629950857016E-4	1.821290982651748E-5
60-61	0.05296769500296947	2.6712267745558974E-4	1.5177424855431234E-5	2.6256944999896034E-4	1.821290982651748E-5
62-63	0.06745454702747858	2.6712267745558974E-4	1.669516734097436E-5	2.656049349700466E-4	1.9730652312060605E-5
64-65	0.08539881643405492	2.6712267745558974E-4	1.821290982651748E-5	2.7622913236884843E-4	2.1248394797603728E-5
66-67	0.10776730518598948	2.7471138988330535E-4	1.821290982651748E-5	2.8078235982547783E-4	2.4283879768689974E-5
68-69	0.13543575069744063	2.7774687485439156E-4	1.821290982651748E-5	2.8837107225319344E-4	2.4283879768689974E-5
70-71	0.16977163894788272	2.8230010231102096E-4	1.821290982651748E-5	2.929242997098228E-4	2.5801622254233098E-5
72-73	0.21129252012488592	2.944420421953659E-4	2.1248394797603728E-5	2.9595978468090905E-4	3.0354849710862467E-5
74-75	0.2617013012972297	3.0051301213753845E-4	2.1248394797603728E-5	3.0354849710862466E-4	3.339033468194872E-5
76-77	0.3226250024094162	3.0658398207971093E-4	2.1248394797603728E-5	3.0354849710862466E-4	3.642581965303496E-5
78-79	0.395560117552191	3.111372095363403E-4	2.4283879768689974E-5	3.0961946705079714E-4	3.642581965303496E-5
80-81	0.48127765990821	3.156904369929697E-4	2.5801622254233098E-5	3.20243664449599E-4	3.642581965303496E-5
82-83	0.5824807288442255	3.2935011936285777E-4	2.731936473977622E-5	3.2176140693514216E-4	3.946130462412121E-5
84-85	0.6999039117232402	3.3390334681948717E-4	2.8837107225319344E-5	3.232791494206853E-4	4.2496789595207456E-5
86-87	0.8337460328108606	3.44527544218289E-4	3.0354849710862467E-5	3.247968919062284E-4	4.2496789595207456E-5
88	0.9474932433898899	3.4604528670383213E-4	3.0354849710862467E-5	3.247968919062284E-4	4.55322745662937E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	23155	0.0	45.242466	1
TCTCGAG	27890	0.0	37.48258	2
TATCTCG	9715	0.0	34.70682	2
CTCGAGG	35565	0.0	29.187021	3
GATCCGG	40900	0.0	25.854956	1
TCGAGGG	43265	0.0	23.97203	4
ATCCGGC	46425	0.0	22.740654	2
ATATCTC	15610	0.0	21.907978	1
TCCGGCG	53060	0.0	19.97988	3
ATATGGA	23600	0.0	18.935747	1
TATGGAT	23515	0.0	18.618437	2
CGAGGGC	57150	0.0	18.156883	5
GCGCCGT	64200	0.0	16.405165	9
AGGGCGC	64165	0.0	16.05819	7
GAGGGCG	65220	0.0	15.889969	6
CGGCGCG	66775	0.0	15.842504	5
GGATCCG	28335	0.0	15.683648	2
CCGGCGC	69915	0.0	15.224466	4
GGGCGCG	71290	0.0	14.591803	8
CGTCGAC	61765	0.0	14.133485	12-13
>>END_MODULE