FastQCFastQC Report
Tue 31 May 2016
SRR212956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530
Sequences flagged as poor quality0
Sequence length58
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC24415.947712418300652No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1368.88888888888889No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1288.366013071895424No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA926.0130718954248366No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT493.202614379084967No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC231.5032679738562091No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT201.3071895424836601No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAGCTGGTTGATGCTGAAAAACATCGAACT161.045751633986928No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.9803921568627451No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT150.9803921568627451No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGATGG130.8496732026143791No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.522875816993464No Hit
CCTTTCGGGCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGAC60.39215686274509803No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGGTGATGCTGAAAAACATCGAAC60.39215686274509803No Hit
CCTTTCGGGGGGAGTTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC50.32679738562091504No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGGTGG50.32679738562091504No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG50.32679738562091504No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA40.261437908496732No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.261437908496732No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.261437908496732No Hit
CCTTTCGGGGGACTGGGTATTCGCTTTTTAAAACACGTCGCTCGTACCCGTCGATTAT40.261437908496732No Hit
CCTTTCGGGCCCGTTGCAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCAT40.261437908496732No Hit
CCTTTCGGGAGGGGAGTCGCCAAGTTGGTCAAGGCACCGGATTGTGATTCCGGCATGC40.261437908496732No Hit
CCTTTCGGGGTAACAGCAATGAACCCTAGAATTATATCCTGTGTACCTCACTGTCCAC40.261437908496732No Hit
CCTTTCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.261437908496732No Hit
CCTTTCGGGGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTA40.261437908496732No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG40.261437908496732No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGG30.19607843137254902No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG30.19607843137254902No Hit
CCTTTCGGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATT30.19607843137254902No Hit
CCTTTCGGGGAGTGTTACGGCACTCTGTGTAAAGCCACTAAGGCTTATAAAAAATTTA30.19607843137254902No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCAGAAGATG30.19607843137254902No Hit
CCTTTCGGGGGGAATTCCTTCGAGTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG30.19607843137254902No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGAT30.19607843137254902No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAGGCGC30.19607843137254902No Hit
CCTTTCGGGATCGCCTCTTTTCCATGCTTCTAGCTCTAGCTATCGGTGTTTTGGCTTT30.19607843137254902No Hit
CCTTTCGGGGTGTGGCCACAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19607843137254902No Hit
CCTTTCGGGGAGTGCAGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCG30.19607843137254902No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGATG30.19607843137254902No Hit
CCTTTCGGGAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCA30.19607843137254902No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAG30.19607843137254902No Hit
CCTTTCGGGGGGAATTCCTTCGGCAAAATTAACTGGTTGATGCTGAAAAACATCGAAC30.19607843137254902No Hit
CCTTTCGGGGCACACACCTGTAGTACCAGCTACTCAGGAGGCTGAGGCAGAAGAATTG30.19607843137254902No Hit
CCTTTCGGGGAGGCACAGTTGCTTGAACCCAGGCGGCAGAGTTTGCAGTGAGGTGAGA30.19607843137254902No Hit
CCTTTCGGGAAGCAGTGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGGAGCGCCGGGAGATG20.130718954248366No Hit
CCTTTCGGGGTTGTCCCGGTTTAAGCATGTAGGCGGAACGATTAGGTAAATCCGGTCG20.130718954248366No Hit
CCTTTCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTT20.130718954248366No Hit
CCTTTCGGGGGCGGAGGTCGCAGTGAGCTGAGATCTTGCCATTGCACTCCAGCCCAGG20.130718954248366No Hit
CCTTTCGGGGTGCTCCTTTCGGGAAGCAGTGGTAATGCCTGGCATGTGCAGTATCCTA20.130718954248366No Hit
CCTTTCGGGAGCCTGAAGACATCTAAAGCTTTGTTTTAAACAGCACCACCTCTATTGA20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACCGGTTGATGCTGAAAAACATCGAAC20.130718954248366No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGCTGGG20.130718954248366No Hit
CCTTTCGGGGTGTGGCCATTAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTG20.130718954248366No Hit
CCTTTCGGGATTTCAACATTTTGAATACATCATTGTTAATTCTGAGTTGGCAGAGGTA20.130718954248366No Hit
CCTTTCGGGAAACGAGAGTTCTGCATAGATAAAGATTTAGCAAAATCTACGTTTTCTA20.130718954248366No Hit
CCTTTCGGGAAATTACTCACTCTCTGTTATTCTGTCACAATACTATAAAACAGACTAA20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGGAGCGCCGGAAGATG20.130718954248366No Hit
CCTTTCGGGGTGTGGCCATCAAATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG20.130718954248366No Hit
CCTTTCGGGATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAA20.130718954248366No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCCGGCCGCCTTTTATATGGG20.130718954248366No Hit
CCTTTCGGGTCCCAAATCTTCTCCTTGCTTTTATTCTCAGCATACTCCAATTTATCTC20.130718954248366No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCAGGAAGAT20.130718954248366No Hit
CCTTTCGGGGGACAGAGTGGCACGACATATTCAAAGTTCTGAAGAATATAAAATTTGT20.130718954248366No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGGAGA20.130718954248366No Hit
CCTTTCGGGCGGGGGGAAGTGAAACATCTCAGTACCCCTAGGAACAGAAATCAACCGA20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATTGAAC20.130718954248366No Hit
CCTTTCGGGATGTAAGGGCGTATGGGGGATGCCTAGGCTCTCAGAGGCGAAGAAGGAC20.130718954248366No Hit
CCTTTCGGGGTCAACTGCGGGAGTGGTTGGAGAGGACAATGGGAGACAAATAAGAGGA20.130718954248366No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAGGGGA20.130718954248366No Hit
CCTTTCGGGCTGGCCTGCAGAATGGTGAGAGAATCCATTTCTGTTGTGTATGCAGCTC20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAAC20.130718954248366No Hit
CCTTTCGGGATCTAAGTAACGGGAGGAAAAGAAAGTAAAAACGATTCCCTAAGTAGCG20.130718954248366No Hit
CCTTTCGGGTTTTTTTGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGC20.130718954248366No Hit
CCTTTCGGGCAGCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.130718954248366No Hit
CCTTTCGGGCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCA20.130718954248366No Hit
CCTTTCGGGAGAAATATCGTATTGGATGTATCCGGATATGGACAAGGATCCAATACAC20.130718954248366No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGGAAAACATCGAAC20.130718954248366No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG20.130718954248366No Hit
CCTTTCGGGGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTG20.130718954248366No Hit
CCTTTCGGGGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG20.130718954248366No Hit
CCTTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC20.130718954248366No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT20.130718954248366No Hit
CCTTTCGGGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGATGT20.130718954248366No Hit
CCTTTCGGGATGTACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.130718954248366No Hit
CCTTTCGGGCGTGAACGGCCGTAAAAGACGTATGCGTTGCCGTGCGAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGGTCCGGGATAAGAGTCCAGCCAATGAAATTTCTAGTGTTCTTTGGGCCA20.130718954248366No Hit
CCTTTCGGGATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGG20.130718954248366No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC20.130718954248366Illumina Single End Adapter 2 (96% over 25bp)
CCTTTCGGGGTGTGGCCATCAAGATCCCCAGTCCGTGGATCTCTGGAGCCTGAATTCT20.130718954248366No Hit
CCTTTCGGGGATGTGATCCGCATCCGCACCGGCGAGACCGGCGCTCAAGCCGTCTGAA20.130718954248366No Hit
CCTTTCGGGTAGACCAGTTGCGACAACTATGGTAATGGTATCATTTATTTTTATTGGA20.130718954248366No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGATGTGTAATTATATAAAGCTGAACAGGGACGGGAAGGGATTCTTTTTAC20.130718954248366No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG20.130718954248366No Hit
CCTTTCGGGGACCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGATCTAAGTAGCCGGAGGAAAAGAAAGTAAAAACGATTCCCTGAGTAGCG20.130718954248366No Hit
CCTTTCGGGGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA20.130718954248366No Hit
CCTTTCGGGAAGCATTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGGAGGCAGGAGAATCACTTGAACCCGGGAGGCGGAGGTTGCCGTGAGCCA20.130718954248366No Hit
CCTTTCGGGTGTGCTCAAAGTTCCAAGATAACTCTGGGCACACAGGACCAACGGGCCT20.130718954248366No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGGGTGTGGCCATCTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGATGGTACAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTTTTTT20.130718954248366No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.130718954248366No Hit
CCTTTCGGGAAGCATGAGCAACCACTCGCACAGTAGTCAGCTCCCGGTCGTACTTGCT20.130718954248366No Hit
CCTTTCGGGTTTCCAGTCAAGAAGAAAAGGGCACAAGGTGGATGCCTTGGCACATGAA20.130718954248366No Hit
CCTTTCGGGGGGGAATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACT20.130718954248366No Hit
CCTTTCGGGGGGCCTTCCTTCTGCAGGAAGGCGAGGATCATGCCGACTGCCTTTTCTG20.130718954248366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC259.873227E-652.044
CCGGAAG259.873227E-652.049
TCGATTC150.005900179552.020
TTGTAGC259.873227E-652.041
CCAAAAA150.005900179552.028
TTCGATT150.005900179552.019
TGTTTTG259.873227E-652.027
TTCTGTT150.005900179552.024
ACCCGTT259.873227E-652.036
GTTTTGC259.873227E-652.028
CCGTTGT259.873227E-652.038
CCCGTTG259.873227E-652.037
TCTGTTT259.873227E-652.025
CGGAAGA259.873227E-652.050
CCCAAAA150.005900179552.027
GCCGGAA259.873227E-652.048
GCGCCGG259.873227E-652.046
CCTTCGA259.873227E-652.017
CGGGAAG150.005900179552.06
CCTTTCG1500.052.01
Produced by FastQC (version 0.11.5)