FastQCFastQC Report
Tue 31 May 2016
SRR212955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611
Sequences flagged as poor quality0
Sequence length58
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC24715.332091868404717No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1398.628181253879578No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1348.317815021725636No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA945.8348851644941035No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT402.4829298572315333No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC301.86219739292365No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT251.5518311607697082No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA191.1793916821849781No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT160.9931719428926132No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGATGG140.8690254500310366No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAGCTGGTTGATGCTGAAAAACATCGAACT100.6207324643078833No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA90.5586592178770949No Hit
CCTTTCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG50.31036623215394166No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG50.31036623215394166No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGGTGATGCTGAAAAACATCGAAC50.31036623215394166No Hit
CCTTTCGGGGGCGGAGGTCGCAGTGAGCTGAGATCTTGCCATTGCACTCCAGCCCAGG40.2482929857231533No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2482929857231533No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCAGGAAGAT40.2482929857231533No Hit
CCTTTCGGGGTGTGGCCATCAAGACCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2482929857231533No Hit
CCTTTCGGGATCGTATGATGAATACATAGTCATACGAGGCGAACGAGGGGAAGTGAAA40.2482929857231533No Hit
CCTTTCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGCGGGTGTTCTGCTGCCGAAGATTCCGGGCAAACTGGGAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGGAGCATTTGATATGG30.186219739292365No Hit
CCTTTCGGGACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGAC30.186219739292365No Hit
CCTTTCGGGGTTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGGTGG30.186219739292365No Hit
CCTTTCGGGGAGTGTTACGGCACTCTGTGTAAAGCCACTAAGGCTTATAAAAAATTTA30.186219739292365No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGAT30.186219739292365No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT30.186219739292365No Hit
CCTTTCGGGATAGTCAAGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCAGAGGC30.186219739292365No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGAAGTTGTTAAGGGCACACGGTGGATGCCTTGGCATCAGGAGCCGATGAA30.186219739292365No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.186219739292365No Hit
CCTTTCGGGCCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.186219739292365No Hit
CCTTTCGGGGTTGTCCCGGTTTAAGCATGTAGGCGGAACGATTAGGTAAATCCGGTCG20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGC20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGG20.12414649286157665No Hit
CCTTTCGGGCGATATCAACCCATCAGTGCTAACGCATTATTGGGGGGGCGATAAAATG20.12414649286157665No Hit
CCTTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG20.12414649286157665No Hit
CCTTTCGGGACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTC20.12414649286157665No Hit
CCTTTCGGGGTGCTCCTTTCGGGAAGCAGTGGTAATGCCTGGCATGTGCAGTATCCTA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGACTCGTCTGGCAGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAAACCCTCTCTACTTTGGCGCGACTGCGTG20.12414649286157665No Hit
CCTTTCGGGCTTGATCTCAGATAAAGTAAGAGAACCCGCCGAATTTAAGCATATTATT20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGA20.12414649286157665No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.12414649286157665No Hit
CCTTTCGGGCAGAGTGCAGTGCTCCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGTGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG20.12414649286157665No Hit
CCTTTCGGGGGGAGTTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC20.12414649286157665No Hit
CCTTTCGGGAAGCATGGTATCCCAAAAGTCCCCTATAACCTTCCCCCGGCCCTGCTCC20.12414649286157665No Hit
CCTTTCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGC20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGCTCCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGAAATTACTCACTCTCTGTTATTCTGTCACAATACTATAAAACAGACTAA20.12414649286157665No Hit
CCTTTCGGGCCCGTTGCAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCAT20.12414649286157665No Hit
CCTTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGCTGGCAGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAAATGGTTGATGCTGAAAAACATCGAAC20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCCGCATTTGATATGGG20.12414649286157665No Hit
CCTTTCGGGGCAATAAAGGCGCTGCATTGACGGGGCTGACCCAACATTTAGGTTTATC20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCCACCCGTTGTAGCGCCGGAAGAC20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAATGCTTC20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTATATGG20.12414649286157665No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT20.12414649286157665No Hit
CCTTTCGGGAGGGGAGTCGCCAAGTTGGTCAAGGCACCGGATTGTGATTCCGGCATGC20.12414649286157665No Hit
CCTTTCGGGGAATAATGAACTAACACCCAGGGCACCTTCCTCACTTGTCTCCTAGCCA20.12414649286157665No Hit
CCTTTCGGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGACAGAGTGGCACGACATATTCAAAGTTCTGAAGAATATAAAATTTGT20.12414649286157665No Hit
CCTTTCGGGCGGGGGGAAGTGAAACATCTCAGTACCCCTAGGAACAGAAATCAACCGA20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.12414649286157665No Hit
CCTTTCGGGATCTAAGTAACGGGAGGAAAAGAAAGTAAAAACGATTCCCTAAGTAGCG20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAGGCGC20.12414649286157665No Hit
CCTTTCGGGATCGCCTCTTTTCCATGCTTCTAGCTCTAGCTATCGGTGTTTTGGCTTT20.12414649286157665No Hit
CCTTTCGGGAGAACACATCCCTTCTCTCATCTCATTGAACAGAACTTAGCTATATGGC20.12414649286157665No Hit
CCTTTCGGGATCTAAAGTTAAGAAAGCAGTAACAAATAATAGTTTAATACGGTTACTG20.12414649286157665No Hit
CCTTTCGGGGAGTGCAGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCG20.12414649286157665No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGATG20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGTCCGGGATAAGAGTCCAGCCAATGAAATTTCTAGTGTTCTTTGGGCCA20.12414649286157665No Hit
CCTTTCGGGGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGA20.12414649286157665No Hit
CCTTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGAGTACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCCGGCAGCATTTGATATGG20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGCTGGCCGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCATCTGCGTGCGAAGAGGGGGCAGCCGTCTCGTTGCAATGCG20.12414649286157665No Hit
CCTTTCGGGACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCT20.12414649286157665No Hit
CCTTTCGGGGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCC20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCCGGCAGCATTTGATATGGA20.12414649286157665No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGCCAT20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAGTCCGTGGATCTCTGGAGCCTGAATTCT20.12414649286157665No Hit
CCTTTCGGGGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATC20.12414649286157665No Hit
CCTTTCGGGGGATACTGGCTTCTTTCTGTTCCTTTATGAAGTCCCAGGCACGCTTATG20.12414649286157665No Hit
CCTTTCGGGGGTGTGGCCATCAGATCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGATGGCAAGGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAACT20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCCGGAAGAT20.12414649286157665No Hit
CCTTTCGGGATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGG20.12414649286157665No Hit
CCTTTCGGGGCAGAGTGCAGTTCTCTGTGTGGGGAGGTACCCCTCACTAAAGGCACTG20.12414649286157665No Hit
CCTTTCGGGCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGAC20.12414649286157665No Hit
CCTTTCGGGACATGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12414649286157665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGCTGGCAGGATTTGATATGG20.12414649286157665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGGAACTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC20.12414649286157665No Hit
CCTTTCGGGAAATGAAGAAAAGAAAGTTTTGACATCTAAATGGGAACTTGAAACAAGA20.12414649286157665No Hit
CCTTTCGGGATTAAAAGTGATTAGTACAACTGCTAGTTATTTTCAACAAATAAAGCCA20.12414649286157665No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTGCGCGAAGTGGTATGGCCGACAGATGTG20.12414649286157665No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTATATGGG20.12414649286157665No Hit
CCTTTCGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12414649286157665No Hit
CCTTTCGGGCAGTGCTCGGGAAGTGGTCACCAGAGTCGACTTTTTTTTTTTTTTTTTT20.12414649286157665No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCCGGGCGCATTTTGTATGGG20.12414649286157665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG304.0384293E-752.06
ATATGGA150.00597825252.052
CGCATTT150.00597825252.044
TTTATAT150.00597825252.049
TTCGGGC150.00597825252.04
TCGGGGT304.0384293E-752.05
CCTTTCG1650.050.4242441
TTTCGGG1650.050.4242443
CTTTCGG1650.050.4242442
TCGGGGG1050.049.523815
CGGGGGG900.049.111116
TTCGGGG1450.048.4137954
CAAGATC351.1725151E-644.57142620
TCAAGAT351.1725151E-644.57142619
TTCGTGG351.1725151E-644.57142615
AATTCGT351.1725151E-644.57142613
TCGTGGA351.1725151E-644.57142616
CATCAAG351.1725151E-644.57142617
TGGAGAA351.1725151E-644.57142619
CGTGGAG351.1725151E-644.57142617
Produced by FastQC (version 0.11.5)