FastQCFastQC Report
Tue 31 May 2016
SRR212954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659
Sequences flagged as poor quality0
Sequence length58
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC22913.803496081977096No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1539.22242314647378No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1257.53465943339361No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA925.545509342977698No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT513.074141048824593No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC342.049427365883062No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT231.3863773357444245No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT211.2658227848101267No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAGCTGGTTGATGCTGAAAAACATCGAACT201.2055455093429777No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.7233273056057866No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA100.6027727546714888No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGATGG100.6027727546714888No Hit
CCTTTCGGGGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAA80.48221820373719104No Hit
CCTTTCGGGGAGTGTTACGGCACTCTGTGTAAAGCCACTAAGGCTTATAAAAAATTTA60.3616636528028933No Hit
CCTTTCGGGCGATATCAACCCATCAGTGCTAACGCATTATTGGGGGGGCGATAAAATG50.3013863773357444No Hit
CCTTTCGGGAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTC50.3013863773357444No Hit
CCTTTCGGGGGGAATTTCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC50.3013863773357444No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAAC50.3013863773357444No Hit
CCTTTCGGGGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTG50.3013863773357444No Hit
CCTTTCGGGTAGACCAGTTGCGACAACTATGGTAATGGTATCATTTATTTTTATTGGA50.3013863773357444No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3013863773357444No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT50.3013863773357444No Hit
CCTTTCGGGACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTC40.24110910186859552No Hit
CCTTTCGGGGGGGACCACTTTGTACAAGAAAGCTGGGTGTTACTTCGCTGTCATCAGG40.24110910186859552No Hit
CCTTTCGGGATCGCCTCTTTTCCATGCTTCTAGCTCTAGCTATCGGTGTTTTGGCTTT40.24110910186859552No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAC40.24110910186859552No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG40.24110910186859552No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAATAAAAAAAAAAAA30.18083182640144665No Hit
CCTTTCGGGAGATTACACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCG30.18083182640144665No Hit
CCTTTCGGGCCCGTTGCAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCAT30.18083182640144665No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGGTTTGCTACCCGTTGTAGCGCCGGAAGATG30.18083182640144665No Hit
CCTTTCGGGGACCACGACTTCAACGTCCTGAGTACCTTCTCCTCACTTACTCCGTAGC30.18083182640144665No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGAT30.18083182640144665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAGGCGC30.18083182640144665No Hit
CCTTTCGGGAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCG30.18083182640144665No Hit
CCTTTCGGGATCTAAAGTTAAGAAAGCAGTAACAAATAATAGTTTAATACGGTTACTG30.18083182640144665No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGATATGG30.18083182640144665No Hit
CCTTTCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCATGCCATGGTGAAA30.18083182640144665No Hit
CCTTTCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG30.18083182640144665No Hit
CCTTTCGGGGCAGCAGGCCGGAGAGTATGCTGGCCAGGCCAAGGATCAGGCAGGCAAC30.18083182640144665No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
CCTTTCGGGCAATCGTTGCTAATAAATGACCAACACTACTTGTGCTCAGAGCGCTGGT30.18083182640144665No Hit
CCTTTCGGGGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTG30.18083182640144665No Hit
CCTTTCGGGCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGAC30.18083182640144665No Hit
CCTTTCGGGAAGTTGTTAAGGGCACACGGTGGATGCCTTGGCATCAGGAGCCGATGAA30.18083182640144665No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGGTGATGCTGAAAAACATCGAAC30.18083182640144665No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGTTG20.12055455093429776No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGG20.12055455093429776No Hit
CCTTTCGGGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTT20.12055455093429776No Hit
CCTTTCGGGGGCGGAGGTCGCAGTGAGCTGAGATCTTGCCATTGCACTCCAGCCCAGG20.12055455093429776No Hit
CCTTTCGGGGTGCTCCTTTCGGGAAGCAGTGGTAATGCCTGGCATGTGCAGTATCCTA20.12055455093429776No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTTCGGGCTACTCAGGAGGCTGAGGCAGGAGAATCGGGAAGGACGTTGGTGTAGAG20.12055455093429776No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGGCGCCTTTTATATGG20.12055455093429776No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12055455093429776No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGATATGGA20.12055455093429776No Hit
CCTTTCGGGGTGTGGCCATCTAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTTCGGGAAGTTATTTTTCAGCCAGTTTGTAAAATAAACTTACTTAATCCTTTCTT20.12055455093429776No Hit
CCTTTCGGGATGGACCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12055455093429776No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT20.12055455093429776No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACAATAACTGGTTGATGCTGAAAAACATCGAAC20.12055455093429776No Hit
CCTTTCGGGGGGAATTACTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG20.12055455093429776No Hit
CCTTTCGGGTCCCAAATCTTCTCCTTGCTTTTATTCTCAGCATACTCCAATTTATCTC20.12055455093429776No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCAGGAAGAT20.12055455093429776No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGGAGA20.12055455093429776No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGTAC20.12055455093429776No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCAAA20.12055455093429776No Hit
CCTTTCGGGCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGGGAC20.12055455093429776No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.12055455093429776No Hit
CCTTTCGGGGGTGGCACACGCCTATAATCCCAGCTACTTGGGAGGCAGAGGTAGGAGA20.12055455093429776No Hit
CCTTTCGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC20.12055455093429776No Hit
CCTTTCGGGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACTGGGAGGCAGAGG20.12055455093429776No Hit
CCTTTCGGGGGGAACCCGGGAGGCAGAGATTGCAGTGAGCCGAGATCGTGCCACTGCA20.12055455093429776No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGATG20.12055455093429776No Hit
CCTTTCGGGGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGA20.12055455093429776No Hit
CCTTTCGGGATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGG20.12055455093429776No Hit
CCTTTCGGGCGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAAT20.12055455093429776No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGGAC20.12055455093429776No Hit
CCTTTCGGGAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAA20.12055455093429776No Hit
CCTTTCGGGAAATGAAGAAAAGAAAGTTTTGACATCTAAATGGGAACTTGAAAAAGAA20.12055455093429776No Hit
CCTTTCGGGGGGAATTGCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC20.12055455093429776No Hit
CCTTTCGGGGTGTGGCCACCAAGATCCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTTCGGGGTGTGGCTATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTTCGGGGCAGAGTGCAGTTCTCTGTGTGGGGAGGTACCCCTCACTAAAGGCACTG20.12055455093429776No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTATATGGA20.12055455093429776No Hit
CCTTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12055455093429776No Hit
CCTTTCGGGGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTA20.12055455093429776No Hit
CCTTTCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG20.12055455093429776No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAATAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTTCGGGCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGC20.12055455093429776No Hit
CCTTTCGGGCACTCTGCCGGCGGCACCTCTTCTTCCTGCGCCCAGCACAGCCACCGTA20.12055455093429776No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC20.12055455093429776Illumina Single End Adapter 2 (100% over 31bp)
CCTTTCGGGTCCCAAATCTCCTCCTTGCTTTTATTCTCAGCATACTCCAATTTATCTC20.12055455093429776No Hit
CCTTTCGGGGGGGAATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACT20.12055455093429776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGT251.0380167E-552.0000045
CGGGGGG950.052.06
CGGGGGA150.00601393652.06
CATCAAG150.00601393652.017
CCATCAA150.00601393652.016
GGCCATC150.00601393652.014
GCCATCA150.00601393652.015
GGGGTGT150.00601393652.07
GTGGCCA150.00601393652.012
ATCAAGA150.00601393652.018
ATCGAAC452.1827873E-1152.052
TTCGGGG1350.052.04
TTCGGGA150.00601393652.04
TGGCCAT150.00601393652.013
TCGGGGG1100.052.05
TCGGGAA150.00601393652.05
CCTTTCG1650.051.9999961
TTTCGGG1650.051.9999963
CTTTCGG1650.051.9999962
GTTGATG506.002665E-1146.80000336
Produced by FastQC (version 0.11.5)