FastQCFastQC Report
Tue 31 May 2016
SRR212953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA85814.024190912062764No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA4968.107224583197123No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG3435.606407322654462No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2443.9882314481856813No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2203.5959463877084015No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1772.893102321019941No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1522.484472049689441No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1151.8796992481203008No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAGCTGGTTGATGCTGAAAAACATCGAA991.6181758744687806No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT631.0297482837528604No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA310.5067015364498202No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.3105590062111801No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGGT180.2942137953579601No Hit
CCTTTCGGGAAATTACTCACTCTCTGTTATTCTGTCACAATACTATAAAACAGACT140.2288329519450801No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGATATG130.21248774109186006No Hit
CCTTTCGGGATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTC130.21248774109186006No Hit
CCTTTCGGGCCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1961425302386401No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1961425302386401No Hit
CCTTTCGGGGTTGTCCCGGTTTAAGCATGTAGGCGGAACGATTAGGTAAATCCGGT120.1961425302386401No Hit
CCTTTCGGGATCTAAGTAACGGGAGGAAAAGAAAGTAAAAACGATTCCCTAAGTAG110.17979731938542007No Hit
CCTTTCGGGACAGTGGTATCAACAGAGTCGACTTTTTTCTTTTTTTTTTTTTTTTT100.16345210853220007No Hit
CCTTTCGGGACTGTCACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.16345210853220007No Hit
CCTTTCGGGGAGCTGTAAGTAAGCAAAGAGCCGTAGATATCCGAATGGGGGAACCC100.16345210853220007No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.16345210853220007No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGGA100.16345210853220007No Hit
CCTTTCGGGAAACGAGAGTTCTGCATAGATAAAGATTTAGCAAAATCTACGTTTTC100.16345210853220007No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGA90.14710689767898005No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGGTGATGCTGAAAAACATCGA90.14710689767898005No Hit
CCTTTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.14710689767898005No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCAGGAAG90.14710689767898005No Hit
CCTTTCGGGATCTAAAGTTAAGAAAGCAGTAACAAATAATAGTTTAATACGGTTAC90.14710689767898005No Hit
CCTTTCGGGTTTCCAGTCAAGAAGAAAAGGGCACAAGGTGGATGCCTTGGCACATG90.14710689767898005No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCCGGCAGCATTTGATATG80.13076168682576006No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCCGGCAGCATTTGATATGG80.13076168682576006No Hit
CCTTTCGGGTACCTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAA80.13076168682576006No Hit
CCTTTCGGGTCCCAAATCTTCTCCTTGCTTTTATTCTCAGCATACTCCAATTTATC80.13076168682576006No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG80.13076168682576006No Hit
CCTTTCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG80.13076168682576006No Hit
CCTTTCGGGGTGCTCCTTTCGGGAAGCAGTGGTAATGCCTGGCATGTGCAGTATCC80.13076168682576006No Hit
CCTTTCGGGAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCT80.13076168682576006No Hit
CCTTTCGGGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTG70.11441647597254005No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGATATGG70.11441647597254005No Hit
CCTTTCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGG70.11441647597254005No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACCGGTTGATGCTGAAAAACATCGA70.11441647597254005No Hit
CCTTTCGGGGGTTAATATTCCGATACTTGTTTATGATGCGATGGAGGGACGGAGAA70.11441647597254005No Hit
CCTTTCGGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACA70.11441647597254005No Hit
CCTTTCGGGAAGCATGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT70.11441647597254005No Hit
CCTTTCGGGGGGGAATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAA70.11441647597254005No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA70.11441647597254005No Hit
CCTTTCGGGCGGGGGGAAGTGAAACATCTCAGTACCCCTAGGAACAGAAATCAACC70.11441647597254005No Hit
CCTTTCGGGATTAAGTGGTAGGGGAGCATTGTGTAAGCCTGTGAAGGTGTGTTGTA70.11441647597254005No Hit
CCTTTCGGGAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGG70.11441647597254005No Hit
CCTTTCGGGGGGTTGGTGAGTCTTGAATAGTTCGACCAGAGTGGGCAACATAATGA70.11441647597254005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTAT150.00803712250.049
CTCCTTT150.00803712250.039
GAGTGCA150.00803712250.029
CGGGGTG402.0063453E-950.06
ATTAGCT150.00803712250.027
CGGGGGT150.00803712250.06
CGGGGGA650.050.06
GCTGGTT150.00803712250.031
AGTGGTA800.050.014
TAGCTGG150.00803712250.029
TGTTATG150.00803712250.050
ACATTAG150.00803712250.025
AGCAGTG800.050.011
CATTAGC150.00803712250.026
GCAGTGG800.050.012
AGAGTGC150.00803712250.028
GCTCCTT150.00803712250.038
TTAGCTG150.00803712250.028
AGCTGGT150.00803712250.030
TTCGGGC150.00803712250.04
Produced by FastQC (version 0.11.5)