FastQCFastQC Report
Tue 31 May 2016
SRR212952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA94214.663760896637609No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA5358.328144458281445No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG3415.308219178082192No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2533.9383561643835616No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2183.3935242839352426No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2093.2534246575342465No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1572.4439601494396017No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1181.8368617683686177No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAGCTGGTTGATGCTGAAAAACATCGAA1051.6344956413449563No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT600.933997509339975No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA290.45143212951432127No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.43586550435865506No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGGT190.2957658779576588No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGGA190.2957658779576588No Hit
CCTTTCGGGATCTAAGTAACGGGAGGAAAAGAAAGTAAAAACGATTCCCTAAGTAG150.23349937733499376No Hit
CCTTTCGGGTCCCAAATCTTCTCCTTGCTTTTATTCTCAGCATACTCCAATTTATC140.21793275217932753No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCAGGAAG140.21793275217932753No Hit
CCTTTCGGGATCGTATGATGAATACATAGTCATACGAGGCGAACGAGGGGAAGTGA130.20236612702366125No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGATATG130.20236612702366125No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.20236612702366125No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.20236612702366125No Hit
CCTTTCGGGGGGTTGGTGAGTCTTGAATAGTTCGACCAGAGTGGGCAACATAATGA130.20236612702366125No Hit
CCTTTCGGGGGGGGGAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGAT120.18679950186799502No Hit
CCTTTCGGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACA110.17123287671232876No Hit
CCTTTCGGGAACCCGGAGAACTGAACCATCTAAGTACCCGGAGGAAAAGAAATCAA100.1556662515566625No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAG100.1556662515566625No Hit
CCTTTCGGGGAGCTGTAAGTAAGCAAAGAGCCGTAGATATCCGAATGGGGGAACCC100.1556662515566625No Hit
CCTTTCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG100.1556662515566625No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA90.14009962640099627No Hit
CCTTTCGGGGGGAATTCCTTCGAGTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA90.14009962640099627No Hit
CCTTTCGGGAAGTTGTTAAGGGCACACGGTGGATGCCTTGGCATCAGGAGCCGATG90.14009962640099627No Hit
CCTTTCGGGGGCGGAGGTCGCAGTGAGCTGAGATCTTGCCATTGCACTCCAGCCCA90.14009962640099627No Hit
CCTTTCGGGAAATTACTCACTCTCTGTTATTCTGTCACAATACTATAAAACAGACT90.14009962640099627No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGATATGG90.14009962640099627No Hit
CCTTTCGGGGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATC90.14009962640099627No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTGATATG90.14009962640099627No Hit
CCTTTCGGGGTCAAGCGAACAAGTGCATGTGGTGGATGCCTTGGCGATCACAGGCG90.14009962640099627No Hit
CCTTTCGGGGAGTGTTACGGCACTCTGTGTAAAGCCACTAAGGCTTATAAAAAATT90.14009962640099627No Hit
CCTTTCGGGCCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.12453300124533001No Hit
CCTTTCGGGGCAGCAGGCCGGAGAGTATGCTGGCCAGGCCAAGGATCAGGCAGGCA80.12453300124533001No Hit
CCTTTCGGGTACCTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAA80.12453300124533001No Hit
CCTTTCGGGACAGTGGTATCAACAGAGTCGACTTTTTTCTTTTTTTTTTTTTTTTT80.12453300124533001No Hit
CCTTTCGGGATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTC80.12453300124533001No Hit
CCTTTCGGGCCCGTTGCAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTC80.12453300124533001No Hit
CCTTTCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC80.12453300124533001No Hit
CCTTTCGGGATTAAGTGGTAGGGGAGCATTGTGTAAGCCTGTGAAGGTGTGTTGTA80.12453300124533001No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCTGTTGTAGCGCCGGAAGA80.12453300124533001No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTCTGCTACCCGTTGTAGCGCCGGAAGA70.10896637608966377No Hit
CCTTTCGGGGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTG70.10896637608966377No Hit
CCTTTCGGGATGGCAAGGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAA70.10896637608966377No Hit
CCTTTCGGGGAGTGCAGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCT70.10896637608966377No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAG70.10896637608966377No Hit
CCTTTCGGGCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTG70.10896637608966377No Hit
CCTTTCGGGGGTTAATATTCCGATACTTGTTTATGATGCGATGGAGGGACGGAGAA70.10896637608966377No Hit
CCTTTCGGGACTGTCACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10896637608966377No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG70.10896637608966377No Hit
CCTTTCGGGTTTCCAGTCAAGAAGAAAAGGGCACAAGGTGGATGCCTTGGCACATG70.10896637608966377No Hit
CCTTTCGGGTTTTTTTGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGT70.10896637608966377No Hit
CCTTTCGGGATAGTCAAGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCAGAG70.10896637608966377No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.10896637608966377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCATT203.5953335E-450.75200343
TGATATG203.5953335E-450.75200350
GCAGCAT251.6997918E-550.75200342
GGAAGAT150.00758529950.75200350
AGCATTT203.5953335E-450.75200344
GGCAGCA203.5953335E-450.75200341
TCGGGAA750.049.5625045
AACGCAG402.2009772E-949.562523
CGGGGTG150.00834023549.56256
CGGGGGT251.9614377E-549.56256
CGGGGGA550.049.56256
AGTGGTA402.2009772E-949.562514
GAAGCAG451.05501385E-1049.56259
CGGGCAC150.00834023549.56256
GGGGGTG204.04939E-449.56257
CGGGACT150.00834023549.56256
CGGGAAG550.049.56256
CCTTTCG6400.049.56251
GGAAGCA505.456968E-1249.56258
GGGAAGC550.049.56257
Produced by FastQC (version 0.11.5)