FastQCFastQC Report
Tue 31 May 2016
SRR212951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22633
Sequences flagged as poor quality0
Sequence length58
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9023.985331153625238No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8283.6583749392480005No Hit
CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6302.783546149427827No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1710.7555339548446959No Hit
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1680.7422789731807538No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.7201873370741838No Hit
CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAACT1390.6141474837626474No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.5478725754429373No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1160.5125259576724253No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACAC660.2916095966067247No Hit
CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT580.25626297883621263No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT500.2209163610657005No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.21207970662307252No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.2076613794017585No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC450.1988247249591305No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG430.18998807051650246No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.18556974329518844No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG390.17231476163124643No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.14580479830336235No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA320.14138647108204833No Hit
CGAGTAGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT280.12371316219679229No Hit
CGAGTAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11487650775416428No Hit
CGAGTAGGGCAGTACAACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11045818053285025No Hit
CGAGTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11045818053285025No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG240.10603985331153626No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10603985331153626No Hit
CGAGTAGGGGTGCTCGAGTAGGGGACTAACATAGTGAAACCCCATCTCTACTAAAAAT240.10603985331153626No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10603985331153626No Hit
CGAGTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA230.10162152609022224No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10162152609022224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGG150.007129373552.036
GGTCTGA150.007129373552.034
GATGTGG150.007129373552.09
AGGGTGA150.007129373552.06
AGGGCAA150.007129373552.06
GTCTGAA150.007129373552.035
AGGGATC150.007129373552.06
GAGCATG150.007129373552.09
ATGTGGC150.007129373552.010
GGGAGAT251.6037055E-552.07
CTGAACC150.007129373552.037
GGGGGTG150.007129373552.07
TCGCCTG150.007129373552.035
AAGATGC307.6491233E-752.052
TCTGAAC150.007129373552.036
GGGACTG150.007129373552.07
GGGATGA150.007129373552.07
TGTGGCT150.007129373552.011
GGGGCAG251.6037055E-552.07
TAGGGTA307.6491233E-752.05
Produced by FastQC (version 0.11.5)