FastQCFastQC Report
Tue 31 May 2016
SRR212950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22586
Sequences flagged as poor quality0
Sequence length58
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9164.0556096697068975No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7703.4091915345789428No Hit
CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6162.727353227663154No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2010.8899318161693084No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.6685557424953511No Hit
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1360.6021429203931639No Hit
CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAACT1350.5977153989196847No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.5800053130257682No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1080.4781723191357478No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACAC770.34091915345789425No Hit
CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT610.27007880988222793No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC520.23023111662091564No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT450.19923846630656156No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.18595590188612415No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG410.18152838041264502No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.17710085893916586No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13725316567785353No Hit
CGAGTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1239706012574161No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA280.1239706012574161No Hit
CGAGTAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11511555831045782No Hit
CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11511555831045782No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT250.11068803683697867No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11068803683697867No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG240.10626051536349952No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGTGG240.10626051536349952No Hit
CGAGTAGGGATGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.10183299389002036No Hit
CGAGTAGGGAGTCGAAGACCGTATAATTGCAGGTCGACGGCGTGCCTATTGAAGAATG230.10183299389002036No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA230.10183299389002036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG150.00707023752.1088952
TGCAATG150.00707023752.1088950
GCAATGC150.00707023752.1088951
AAGATGC353.600144E-852.1088952
ATATGGG203.3407702E-452.1088952
ACAAGTG150.00707023752.1088951
GGATGGT203.3777434E-451.993358
ACAAAAG203.3777434E-451.9933512
AGGGTGT203.3777434E-451.993356
GGACATG203.3777434E-451.993358
AGGGACT401.7589628E-951.993356
GACATGG203.3777434E-451.993359
GCAAAAC203.3777434E-451.9933518
GAACAAA203.3777434E-451.9933510
AGCAAAA203.3777434E-451.9933517
AAAGCAA203.3777434E-451.9933515
GGAACAA203.3777434E-451.993359
GGGTGGA203.3777434E-451.993357
CAAAAGC203.3777434E-451.9933513
CAAAACC203.3777434E-451.9933519
Produced by FastQC (version 0.11.5)