FastQCFastQC Report
Tue 31 May 2016
SRR212949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23073
Sequences flagged as poor quality0
Sequence length58
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8113.514930871581502No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6943.0078446669267107No Hit
CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5372.3273956572617345No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.7671304121700689No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1650.7151215706670133No Hit
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1390.6024357474103931No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1360.5894335370346292No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.5504269059073376No Hit
CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAACT1140.49408399427902744No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACAC810.3510596801456248No Hit
CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT570.24704199713951372No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT560.24270792701425908No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC430.18636501538594896No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG420.18203094526069433No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.16902873488493045No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGTGG350.15169245438391193No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.15169245438391193No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG320.13869024400814806No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG290.12568803363238418No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGCTGGCAGCATTTGATATGGA270.11701989338187492No Hit
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCTGTTGTAGCGCCGGAAGATG250.10835175313136566No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA250.10835175313136566No Hit
CGAGTAGGGGTAACAAGAATAAAACAAAAAACTTGCTACAAAATCTCTTTTTAAACAG240.10401768300611104No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT240.10401768300611104No Hit
CGAGTAGGGGTCTCCTGCTTCAGGTTTAACCGTGTCTGCCATCGATTGCCCACCAGCA240.10401768300611104No Hit
CGAGTAGGGTGCTCGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTA240.10401768300611104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG203.2459627E-452.4166686
GGATGGT203.2459627E-452.4166688
GGACTGG353.439527E-852.4166688
GGGTACA203.2459627E-452.4166687
AGGGCTA203.2459627E-452.4166686
AGGGATT401.6370905E-952.4166686
AGGGATC353.439527E-852.4166686
GAGCATG251.530143E-552.4166689
AGGGACT550.052.4166686
AGGGAAT251.530143E-552.4166686
AGGGAAC550.052.4166686
GGGATTG203.2459627E-452.4166687
GGGATGT203.2459627E-452.4166687
GGGAATG203.2459627E-452.4166687
TAGGGTT401.6370905E-952.4166685
ACTGGTA203.2459627E-452.41666810
GGTGCTC503.6379788E-1252.4166689
AGGGGTC150.00690866552.4166646
GGGTTAT150.00690866552.4166647
GACAGTG307.242106E-752.4166649
Produced by FastQC (version 0.11.5)