FastQCFastQC Report
Tue 31 May 2016
SRR212948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22485
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9054.024905492550589No Hit
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA7903.5134534133867024No Hit
CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4762.116966866800089No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1800.8005336891260841No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.7649544140538136No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.6404269513008672No Hit
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1290.5737158105403602No Hit
CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAA1060.4714253947075828No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC670.29797642873026464No Hit
CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA510.22681787858572383No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.2179230598176562No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.20458083166555482No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGT450.20013342228152103No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGG440.1956860128974872No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.1956860128974872No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG400.177896375361352No Hit
CGAGTAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1601067378252168No Hit
CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1467645096731154No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG320.1423171002890816No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGGAGCAGG300.13342228152101399No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1289748721369802No Hit
CGAGTAGGGCAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.12452746275294642No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11563264398487881No Hit
CGAGTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1067378252168112No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10229041583277741No Hit
CGAGTAGGGGTATAGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAG230.10229041583277741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGA505.456968E-1250.17040650
TGGCAGC150.00821250750.17040340
CAGCATT150.00821250750.17040343
CGCCTGG150.00821250750.17040336
TAACGGC150.00821250750.17040337
CATTTGA150.00821250750.17040346
GCAGCAT150.00821250750.17040342
TTGATAT204.0273907E-450.17040349
AGCATTT150.00821250750.17040344
GCATTTG150.00821250750.17040345
CCTGGCA150.00821250750.17040338
GAGTGGA150.00828577450.05816729
AGGGGTA301.0253643E-649.835196
AGGGGCT204.163446E-449.835196
AGGGGAC150.00843376449.835196
AAGAGTG150.00843376449.8351910
AGGGCTA204.163446E-449.835196
AGGGTGT301.0253643E-649.835196
AGGGTCA150.00843376449.835196
GAAGAGT150.00843376449.835199
Produced by FastQC (version 0.11.5)