##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22485 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.0128974872137 38.0 37.0 39.0 33.0 40.0 2 36.36321992439404 38.0 36.0 39.0 31.0 40.0 3 35.01979097175895 38.0 33.0 38.0 27.0 40.0 4 36.32950856126306 38.0 36.0 39.0 31.0 40.0 5 33.984033800311316 36.0 33.0 38.0 25.0 40.0 6 36.8239270624861 38.0 36.0 40.0 33.0 40.0 7 37.088859239493 38.0 37.0 40.0 33.0 40.0 8 37.01712252612853 38.0 37.0 40.0 33.0 40.0 9 36.931776740048925 38.0 37.0 40.0 33.0 40.0 10 35.61458750277963 38.0 35.0 39.0 29.0 40.0 11 36.34098287747387 38.0 36.0 39.0 31.0 40.0 12 36.25621525461419 38.0 35.0 40.0 31.0 40.0 13 36.04358461196353 38.0 35.0 39.0 30.0 40.0 14 36.00751612185902 38.0 35.0 39.0 30.0 40.0 15 36.24780965087837 38.0 35.0 40.0 31.0 40.0 16 35.95517011340894 38.0 35.0 39.0 30.0 40.0 17 35.99919946631088 38.0 35.0 39.0 30.0 40.0 18 35.90776072937514 38.0 35.0 39.0 30.0 40.0 19 35.464220591505445 38.0 35.0 39.0 29.0 40.0 20 35.77513898154325 38.0 35.0 39.0 30.0 40.0 21 35.513853680231264 38.0 35.0 39.0 29.0 40.0 22 35.13786969090505 38.0 35.0 39.0 28.0 40.0 23 34.81778963753614 38.0 34.0 39.0 27.0 40.0 24 34.58363353346675 38.0 33.0 39.0 27.0 40.0 25 34.95637091394263 38.0 34.0 39.0 28.0 40.0 26 34.441271959083835 38.0 34.0 39.0 26.0 40.0 27 34.500289081609964 38.0 34.0 39.0 27.0 40.0 28 33.85207916388703 38.0 33.0 39.0 24.0 40.0 29 34.021347565043364 38.0 33.0 39.0 25.0 40.0 30 33.61596619968868 38.0 33.0 39.0 23.0 40.0 31 33.30620413609073 38.0 33.0 39.0 19.0 40.0 32 32.93413386702246 38.0 32.0 39.0 17.0 40.0 33 33.115232377140316 38.0 33.0 39.0 18.0 40.0 34 33.30224594173894 38.0 33.0 39.0 18.0 40.0 35 32.82993106515455 38.0 33.0 39.0 15.0 40.0 36 32.139737602846346 38.0 33.0 39.0 2.0 40.0 37 31.514076050700467 38.0 32.0 39.0 2.0 40.0 38 31.37135868356682 38.0 32.0 39.0 2.0 40.0 39 31.363931509895487 38.0 32.0 39.0 2.0 40.0 40 31.03117633978208 37.0 31.0 39.0 2.0 40.0 41 30.751612185901713 37.0 31.0 39.0 2.0 40.0 42 30.75917278185457 37.0 31.0 39.0 2.0 40.0 43 30.637269290638205 37.0 31.0 39.0 2.0 40.0 44 30.16700022237047 36.0 29.0 39.0 2.0 40.0 45 30.16717811874583 36.0 30.0 39.0 2.0 40.0 46 30.029441850122303 36.0 30.0 39.0 2.0 40.0 47 29.886368690237937 36.0 29.0 39.0 2.0 40.0 48 29.788837002446076 36.0 29.0 39.0 2.0 40.0 49 29.551834556370913 36.0 29.0 39.0 2.0 40.0 50 29.277162552812985 36.0 28.0 39.0 2.0 40.0 51 28.744096064042694 36.0 26.0 39.0 2.0 40.0 52 28.524349566377584 35.0 25.0 39.0 2.0 40.0 53 27.969312875250168 35.0 19.0 39.0 2.0 40.0 54 27.719146097398266 35.0 2.0 39.0 2.0 40.0 55 27.180564820991773 35.0 2.0 39.0 2.0 40.0 56 15.352901934623082 19.0 2.0 23.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.015737359302463005 1 2 -0.5024462303919606 1 3 0.46648720704155267 1 4 -1.0707308014677892 1 5 -0.8460736147353103 1 6 0.00716921818921179 1 7 -0.4366845068077083 1 8 -0.4385204433588754 1 9 -0.5223138232683056 1 10 -0.3788753807180143 1 11 -0.6296252824236888 1 12 -2.482272688604475 1 13 -0.44921152390030983 1 14 -3.155673548620129 1 15 -2.8808976545867324 1 16 -5.3065599051328896 1 17 -4.801208519809084 1 18 -4.037320181848859 1 19 -5.611777144481941 1 20 -4.592697930565414 1 21 -5.431402769851729 1 22 -6.397931471584798 1 23 -4.073403998995211 1 24 -5.773544251174517 1 25 -4.998695085971363 1 26 -3.0422210004181807 1 27 -4.1130374206160525 1 28 -5.068113133235276 1 29 -5.391004707070941 1 30 -5.275780839120532 1 31 -3.60416150135822 1 32 -3.2504162698009225 1 33 -4.803272447453519 1 34 -3.5357487272045525 1 35 -4.8454396619328755 1 36 -6.468567532557632 1 37 -5.524344041021987 1 38 -4.951492974523507 1 39 -5.616151625299711 1 40 -5.135614814928687 1 41 -6.1829075001799225 1 42 -5.882454464101038 1 43 -6.13922812065065 1 44 -5.356152105743298 1 45 -4.062112581687153 1 46 -5.7759676260034 1 47 -5.631873186463817 1 48 -6.141837156571981 1 49 -5.648527604587194 1 50 -5.1382267336055385 1 51 -6.569593589382471 1 52 -6.941188867891643 1 53 -8.16594695529023 1 54 -7.227003146225183 1 55 -9.103869997196728 1 56 -5.090558686182048 2 1 -0.19220794753776005 2 2 -0.3259756421566635 2 3 -3.0629245576643314 2 4 -0.0707308014677892 2 5 -2.1990147912058937 2 6 0.3012868652480378 2 7 0.5044919637805236 2 8 0.3261854389940666 2 9 0.47768617673169445 2 10 1.3270069722231597 2 11 1.1350805999292533 2 12 0.9883155466896412 2 13 1.962553181982038 2 14 1.726679392556342 2 15 0.9426317571779705 2 16 1.516969506631817 2 17 2.610556186073268 2 18 2.3156209946217317 2 19 2.505869914341588 2 20 1.6425961870816437 2 21 2.6862442889717997 2 22 2.6608920578269704 2 23 2.691301883357731 2 24 1.4029263370607765 2 25 2.589540208146282 2 26 1.4283672348759353 2 27 2.0046096382074765 2 28 2.814239807941192 2 29 1.903112939987885 2 30 2.430101513820645 2 31 3.2193679104064827 2 32 3.9848778478461355 2 33 2.608492258428832 2 34 4.287780684560154 2 35 3.566325043949476 2 36 1.5314324674423645 2 37 1.5933030178015457 2 38 2.3426246725353117 2 39 2.148554257053231 2 40 1.9820322438948423 2 41 2.1700336762906645 2 42 2.646957300604843 2 43 3.1548895264081693 2 44 2.290906717786111 2 45 2.9378874183128474 2 46 3.2240323739966037 2 47 3.0151856370655956 2 48 2.681692255192722 2 49 2.998531218942219 2 50 1.685302678159168 2 51 1.6068769988528224 2 52 2.470575837990708 2 53 1.4222883388274141 2 54 3.42005567730423 2 55 3.8961300028032717 2 56 1.968264843229715 3 1 -1.7480903004789354 3 2 -2.058328583333136 3 3 -3.2776304400172727 3 4 -1.7030837426442602 3 5 -2.187250085323541 3 6 -0.5487131347519636 3 7 -0.9160962715135952 3 8 -0.35910867865299423 3 9 -0.46643147032713017 3 10 -2.196522439541546 3 11 -2.7178605765413337 3 12 -2.6969785709574197 3 13 -3.1286232886061924 3 14 -3.193908842737777 3 15 -2.5926623604690846 3 16 -3.394795199250538 3 17 -3.910032049220849 3 18 -2.6108495936135654 3 19 -2.4264830268348803 3 20 -2.2603449893889405 3 21 -2.263755711028203 3 22 -1.3685197068789137 3 23 -2.8704628225246225 3 24 -2.7294266041156945 3 25 -2.213400968324308 3 26 -2.1451621768887676 3 27 -2.1806844794395843 3 28 -1.7916425449999807 3 29 -1.7115929423650584 3 30 -1.7022514273558258 3 31 -3.2512203248876332 3 32 -3.929828034506805 3 33 -2.679743035688812 3 34 -3.2916310801457307 3 35 -2.036616132521111 3 36 -1.1420969443223399 3 37 -1.000814629257281 3 38 -0.43090473922939054 3 39 -1.0661516252997103 3 40 0.09673812624778577 3 41 -0.3976133825328638 3 42 -0.19127799351280217 3 43 0.0637130558199388 3 44 -0.2649756351550643 3 45 -0.5974066993342113 3 46 1.16226766811425 3 47 0.865185637065597 3 48 0.5434569610750764 3 49 1.1514723954128065 3 50 -0.4735208512525979 3 51 -0.48724064820600077 3 52 0.5793993674024733 3 53 0.036994221180357556 3 54 1.061232147892465 3 55 1.887306473391508 3 56 0.4065001373473631 4 1 -0.7980903004789326 4 2 -0.808328583333136 4 3 -1.5276304400172762 4 4 -1.0530837426442616 4 5 -1.3872500853235437 4 6 -1.0987131347519608 4 7 -1.5160962715135895 4 8 -1.6591086786529914 4 9 -3.8164314703271316 4 10 -3.6965224395415426 4 11 -3.9178605765413366 4 12 -3.246978570957417 4 13 -5.228623288606194 4 14 -3.8439088427377754 4 15 -4.142662360469089 4 16 -2.7447951992505395 4 17 -2.810032049220851 4 18 -3.1608495936135625 4 19 -4.076483026834882 4 20 -2.160344989388946 4 21 -2.263755711028203 4 22 -2.0685197068789165 4 23 -3.0204628225246246 4 24 -4.629426604115693 4 25 -3.9634009683243043 4 26 -1.245162176888769 4 27 -1.2306844794395815 4 28 -2.941642544999983 4 29 -1.861592942365057 4 30 -3.302251427355827 4 31 -0.5012203248876332 4 32 -1.129828034506808 4 33 -1.7797430356888135 4 34 -2.0416310801457307 4 35 -1.7366161325211102 4 36 -3.2420969443223377 4 37 -3.5508146292572818 4 38 -2.030904739229392 4 39 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0.7205733958843084 5 25 0.08659903167569638 5 26 1.8048378231112352 5 27 2.669315520560417 5 28 1.0583574550000208 5 29 1.5384070576349416 5 30 2.197748572644173 5 31 2.4487796751123696 5 32 3.320171965493195 5 33 3.0702569643111843 5 34 4.208368919854273 5 35 1.0633838674788905 5 36 -4.89209694432234 5 37 -4.000814629257281 5 38 -3.780904739229392 5 39 -3.9661516252997124 5 40 -3.603261873752217 5 41 -2.647613382532864 5 42 -3.3912779935128015 5 43 -2.986286944180062 5 44 -2.4649756351550636 5 45 -3.3474066993342113 5 46 -4.237732331885752 5 47 -2.9348143629344037 5 48 -3.0065430389249244 5 49 -3.7985276045871927 5 50 -5.323520851252596 5 51 -4.137240648205999 5 52 -5.320600632597529 5 53 -3.2130057788196424 5 54 -3.0887678521075337 5 55 -3.512693526608494 5 56 -2.9934998626526372 6 1 -0.0033534583736738455 6 2 -0.6293812149120797 6 3 0.6986853494564116 6 4 0.15217941525047962 6 5 0.2653814936238277 6 6 0.4591816020901405 6 7 0.29706162322325014 6 8 0.7224702687154263 6 9 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1.379416581343122 9 6 0.6346201985813735 9 7 0.700570395153072 9 8 0.4242246546803443 9 9 1.016901863006204 9 10 1.614588671569571 9 11 0.22658386790310914 9 12 0.5307992068203617 9 13 0.18804337806047045 9 14 1.0894244905955617 9 15 0.7628931950864697 9 16 1.4940936896383477 9 17 2.139967950779152 9 18 1.6391504063864346 9 19 1.3457391953873383 9 20 2.2896550106110567 9 21 1.1862442889717997 9 22 2.2425914042322006 9 23 1.3906482885864904 9 24 2.298351173662084 9 25 0.9032656983423593 9 26 2.049282267555675 9 27 1.49153774278264 9 28 1.8861352327777965 9 29 2.0272959465238287 9 30 1.8255263504219528 9 31 1.5821130084457025 9 32 2.2757275210487506 9 33 1.6869236309778515 9 34 1.5361466976320486 9 35 0.6022727563677748 9 36 1.98568083345544 9 37 2.6880742596316054 9 38 1.5746508163261659 9 39 2.2171817080336247 9 40 2.13562701513667 9 41 2.9968310619115783 9 42 3.9476108953760836 9 43 3.102601944708823 9 44 4.140579920400494 9 45 2.8692599673324537 9 46 0.701156557003138 9 47 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1.0780624277105701 114 20 1.1987459197019632 114 21 1.5044261071536198 114 22 1.676934838575633 114 23 0.9159008138390092 114 24 2.3842097595206724 114 25 1.7820535771302417 114 26 2.332110550383959 114 27 2.4056791569240517 114 28 2.108357455000018 114 29 1.9111343303622164 114 30 2.775021299916901 114 31 3.52150694783964 114 32 3.7201719654931935 114 33 3.020256964311187 114 34 4.531096192581543 114 35 2.077020231115256 114 36 1.4351757829503882 114 37 4.1628217343790865 114 38 3.791822533497882 114 39 4.656575647427562 114 40 4.155829035338691 114 41 4.643295708376229 114 42 3.195085642850831 114 43 3.9864403285472108 114 44 3.6759334557540306 114 45 2.8844114824839693 114 46 4.403176759023342 114 47 4.560640182520142 114 48 3.6843660519841706 114 49 2.9423814863218993 114 50 4.899206421474677 114 51 4.267304806339453 114 52 3.983944821947926 114 53 4.641539675725809 114 54 4.497595784256099 114 55 3.564579200664234 114 56 0.8292274100746369 115 1 0.5781001757115405 115 2 1.1250047500002012 115 3 1.0771314647446317 115 4 1.2850114954509806 115 5 0.8317975337240782 115 6 1.110810674771848 115 7 1.1291418237245026 115 8 0.9956532261089137 115 9 1.0407113868157225 115 10 0.5034775604584567 115 11 -0.12262248130323883 115 12 0.943497619518773 115 13 0.35471004472714185 115 14 1.256091157262226 115 15 1.231147163340438 115 16 -0.13289043734577888 115 17 0.5685393793505824 115 18 -0.7656114983754705 115 19 0.052088401736547496 115 20 -0.4722497512937025 115 21 -1.4566128538853462 115 22 -1.0113768497360596 115 23 -1.1966532987150984 115 24 -1.1937123184014098 115 25 -1.1919723968957356 115 26 -1.4427812245078186 115 27 -0.9449701937252968 115 28 -0.5583092116666464 115 29 0.3765022957301838 115 30 0.0715580964536997 115 31 0.41544634177903106 115 32 0.2201719654931935 115 33 0.6869236309778515 115 34 -0.14639298490763508 115 35 -0.07233041823539565 115 36 -0.05400170622709766 115 37 1.4420425135998656 115 38 0.4714762131515613 115 39 0.2886102794621941 115 40 1.08007145958112 115 41 1.1000056650861865 115 42 0.9158648636300555 115 43 1.2613321034389884 115 44 0.7755005553211269 115 45 1.4168790149515047 115 46 1.3836962395428216 115 47 2.1104237323036905 115 48 1.9982188658369822 115 49 2.1133771573175686 115 50 2.7217172439854984 115 51 2.979426018460668 115 52 3.8627327007358083 115 53 3.5679466021327357 115 54 3.599327385987703 115 55 4.59921123529627 115 56 0.5565001373473635 116 1 -0.7076141100027442 116 2 -0.35118572619028043 116 3 -0.49429710668393767 116 4 -0.6197504093109245 116 5 1.3556070575336037 116 6 0.5870011509623225 116 7 -0.29942960484692804 116 8 -0.3852991548434659 116 9 -0.8164314703271316 116 10 1.170144227125121 116 11 0.8773775186967612 116 12 0.2768309528521087 116 13 0.11661480663190105 116 14 0.017995919166985175 116 15 -0.43551950332623335 116 16 -0.6090809135362534 116 17 -0.4790796682684686 116 18 -0.9560876888516603 116 19 0.1473264969746424 116 20 -0.1389164179603739 116 21 -0.5518509491234411 116 22 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117 4 -2.0030837426442574 117 5 -4.2372500853235415 117 6 -2.898713134751965 117 7 -1.2660962715135895 117 8 -1.55910867865299 117 9 -1.0664314703271316 117 10 -2.7965224395415405 117 11 -3.317860576541335 117 12 -3.1469785709574154 117 13 -3.228623288606194 117 14 -2.3439088427377754 117 15 -1.5426623604690874 117 16 -1.0447951992505367 117 17 -2.4100320492208454 117 18 -1.760849593613564 117 19 -1.4764830268348845 117 20 -2.0603449893889447 117 21 -2.3137557110282003 117 22 -2.2685197068789122 117 23 -2.220462822524624 117 24 -1.9294266041156973 117 25 -2.263400968324305 117 26 -1.4451621768887648 117 27 -2.13068447943958 117 28 -0.14164254499998208 117 29 -1.2115929423650584 117 30 -0.9022514273558286 117 31 1.9487796751123696 117 32 1.570171965493195 117 33 0.07025696431118433 117 34 -1.5916310801457314 117 35 -0.6866161325211095 117 36 -3.14209694432234 117 37 -1.7008146292572803 117 38 -0.9809047392293913 117 39 -2.7161516252997124 117 40 -2.9032618737522142 117 41 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118 24 2.5732049748316754 118 25 0.18396745272832504 118 26 0.3416799283743899 118 27 0.13773657319200083 118 28 -0.7863793871052422 118 29 1.612091268161258 118 30 -0.5575145852505656 118 31 -0.6722729564665784 118 32 -0.8324596134541764 118 33 0.020256964311187176 118 34 -1.18899950119836 118 35 -1.9445108693632172 118 36 -0.44999168116444466 118 37 -1.8060777871520166 118 38 -2.6387994760714975 118 39 -1.5108884674049747 118 40 -3.042735557962743 118 41 -2.9476133825328645 118 42 -3.257067467197011 118 43 -2.8389185231274325 118 44 -2.993923003576114 118 45 -3.402669857228947 118 46 -2.2929954897804876 118 47 -1.6690248892501955 118 48 -2.6433851441880805 118 49 -1.6485276045871942 118 50 -2.0050997986210177 118 51 -0.8977669639954726 118 52 -1.0495480010185787 118 53 -3.7077426209249076 118 54 -1.7966625889496406 118 55 -1.6890093160821777 118 56 -1.127710388968426 119 1 -0.49809030047893543 119 2 -0.2583285833331317 119 3 0.3223695599827252 119 4 0.6969162573557384 119 5 0.36274991467645634 119 6 0.5012868652480407 119 7 1.1839037284864062 119 8 0.94089132134701 119 9 1.1835685296728684 119 10 1.1534775604584553 119 11 1.4321394234586649 119 12 2.003021429042583 119 13 0.8713767113938076 119 14 1.8560911572622274 119 15 2.0073376395309097 119 16 1.905204800749459 119 17 1.7399679507791532 119 18 1.6391504063864346 119 19 1.5235169731651155 119 20 2.4896550106110595 119 21 1.0862442889717983 119 22 2.6814802931210835 119 23 2.479537177475379 119 24 1.920573395884304 119 25 2.736599031675695 119 26 2.1548378231112295 119 27 3.4693155205604214 119 28 3.158357455000015 119 29 2.9884070576349444 119 30 3.6477485726441756 119 31 2.1487796751123653 119 32 2.570171965493195 119 33 0.6202569643111886 119 34 2.208368919854273 119 35 2.163383867478892 119 36 0.8579030556776601 119 37 2.099185370742717 119 38 2.7690952607706087 119 39 2.7838483747002876 119 40 2.5967381262477858 119 41 1.3523866174671326 119 42 1.9087220064871993 119 43 1.3137130558199388 119 44 2.085024364844937 119 45 1.7525933006657866 119 46 1.0622676681142487 119 47 2.5651856370655928 119 48 1.8934569610750778 119 49 2.4514723954128073 119 50 3.0764791487474064 119 51 3.2127593517939985 119 52 1.9293993674024712 119 53 1.7369942211803568 119 54 2.311232147892465 119 55 3.737306473391506 119 56 0.05650013734736348 120 1 0.10190969952106599 120 2 -0.7708285833331345 120 3 0.29736955998272663 120 4 -2.01558374264426 120 5 0.7752499146764578 120 6 0.05128686524803783 120 7 -1.7160962715135923 120 8 -2.3466086786529914 120 9 -1.4414314703271316 120 10 -1.8090224395415433 120 11 -1.1553605765413337 120 12 -0.7469785709574168 120 13 -1.2911232886061939 120 14 0.256091157262226 120 15 -1.3551623604690874 120 16 -0.08229519925053808 120 17 -0.1725320492208482 120 18 -0.9858495936135654 120 19 -1.751483026834883 120 20 -0.7728449893889433 120 21 -2.5637557110282003 120 22 -0.7435197068789137 120 23 0.3420371774753761 120 24 -3.0419266041156945 120 25 -0.763400968324305 120 26 -4.145162176888768 120 27 -3.6681844794395815 120 28 -5.766642544999982 120 29 -4.799092942365057 120 30 -4.014751427355826 120 31 -5.126220324887633 120 32 -4.0298280345068065 120 33 -4.854743035688813 120 34 -5.42913108014573 120 35 -4.911616132521111 120 36 -5.1670969443223385 120 37 -6.51331462925728 120 38 -7.168404739229391 120 39 -7.116151625299711 120 40 -6.253261873752216 120 41 -5.8226133825328645 120 42 -5.503777993512802 120 43 -5.786286944180063 120 44 -6.289975635155063 120 45 -6.422406699334211 120 46 -4.312732331885751 120 47 -5.047314362934404 120 48 -4.031543038924923 120 49 -4.648527604587194 120 50 -4.5610208512525965 120 51 -4.749740648206 120 52 -4.095600632597527 120 53 -4.000505778819644 120 54 -3.2012678521075344 120 55 -3.600193526608493 120 56 0.18150013734736348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91.0 3 10.0 4 23.0 5 24.0 6 58.0 7 106.0 8 56.0 9 49.0 10 84.0 11 87.0 12 78.0 13 113.0 14 124.0 15 106.0 16 161.0 17 170.0 18 181.0 19 163.0 20 247.0 21 258.0 22 265.0 23 322.0 24 431.0 25 562.0 26 327.0 27 309.0 28 407.0 29 463.0 30 588.0 31 648.0 32 797.0 33 1073.0 34 1301.0 35 1582.0 36 2071.0 37 3371.0 38 3487.0 39 2292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 38.358905937291524 42.96197464976651 7.974205025572605 10.704914387369357 11 30.1578830331332 37.65621525461419 19.773182121414276 12.412719590838336 12 57.90082277073605 17.104736490993997 14.120524794307315 10.873915943962642 13 20.60929508561263 26.622192572826332 20.787191460973983 31.98132088058706 14 22.58394485212364 42.83299977762953 20.062263731376472 14.520791638870358 15 35.952857460529245 20.996219702023573 28.21436513231043 14.836557705136757 16 20.035579275072273 20.68045363575717 40.81387591727819 18.47009117189237 17 34.414053813653545 20.088948187680675 16.268623526795643 29.228374471870133 18 33.47565043362242 20.65376917945297 20.04447409384034 25.826106293084276 19 17.98532354903269 21.583277740716035 41.88570157883033 18.545697131420948 20 21.690015565932843 32.372692906382035 22.672893039804315 23.26439848788081 21 27.600622637313766 19.64865465866133 28.663553480097843 24.087169223927063 22 29.281743384478542 27.218145430286857 15.032243718034245 28.467867467200357 23 31.47876362019124 36.74449633088726 17.580609295085612 14.19613075383589 24 18.994885479208364 39.6842339337336 26.14631976873471 15.174560818323327 25 20.219770442210162 28.014058190230447 25.585016460539194 26.1811549070202 26 31.513614522156967 24.515038263036125 24.862075102331378 19.10927211247553 27 22.055550609810382 20.87599038547138 28.674441378082438 28.394017626635804 28 23.57419527180446 33.40011575619963 30.28805485062998 12.737634121365923 29 27.417271634080077 22.063866743864963 37.67870663163052 12.840154990424443 30 13.903743315508022 32.5 41.99197860962567 11.60427807486631 31 23.223995008467778 21.120420714858724 44.51377128086283 11.141812995810678 32 15.884267308635373 19.602336052784093 52.58347822210334 11.929918416477197 33 22.96851306752297 17.77272321826777 41.11586834359112 18.14289537061814 34 16.693426099366693 17.21969494246722 45.89242708054589 20.194451877620196 35 13.546314474388721 26.865072282705693 48.5900410494378 10.998572193467785 36 16.666666666666664 21.344402785216925 44.148366363149435 17.84056418496697 37 11.251004553978033 17.818555228145367 53.352977944459326 17.577462273417268 38 13.61951132353598 16.522982087818825 51.93192477777282 17.92558181087238 39 13.404226799517449 18.1805996157455 48.366918368258794 20.048255216478264 40 14.914163090128755 19.13447782546495 53.3217095851216 12.629649499284692 41 14.465802413947252 17.93920429146178 55.61019222172553 11.984801072865444 42 19.218547195989796 16.680839636575215 53.461934386608775 10.63867878082621 43 16.408557873064183 19.349207770119058 51.39647301047354 12.84576134634321 44 16.13033164749586 19.357293111936624 55.25220426979367 9.260170970773844 45 15.897895208240037 23.287057769816393 51.69278996865204 9.122257053291536 46 15.15205804631164 23.688807273704484 48.878040041205715 12.281094638778162 47 14.53339904126159 23.050042560817168 49.46014963487299 12.95640876304825 48 17.487563303903553 21.83032313001389 50.63864115089858 10.043472415183974 49 12.979800241859632 21.373225242979352 52.95382272584763 12.693151789313387 50 10.584334110055565 23.552607994264203 52.24950708012188 13.613550815558344 51 15.60973423564738 22.62806435710124 50.09187469188365 11.670326715367722 52 17.316056135945836 23.795005156257005 50.19952472761512 8.689413980182039 53 10.03183999282479 27.324095250908115 52.15032064218127 10.493744114085834 54 11.385250314013996 28.786111609546026 48.7394581015611 11.089179974878881 55 18.359567693618548 24.50782546302525 48.791425624467465 8.341181218888739 56 15.10694587686651 28.554773328550286 47.35662077933725 8.981660015245954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 20.5 11 41.0 12 45.0 13 36.5 14 24.0 15 56.5 16 89.0 17 142.0 18 195.0 19 290.5 20 386.0 21 367.5 22 424.5 23 500.0 24 687.5 25 875.0 26 1056.0 27 1237.0 28 1270.5 29 1369.0 30 1434.0 31 1460.5 32 1487.0 33 1264.0 34 1041.0 35 1286.0 36 1531.0 37 1036.5 38 484.0 39 426.0 40 394.5 41 363.0 42 403.5 43 444.0 44 466.0 45 488.0 46 781.5 47 823.5 48 572.0 49 554.0 50 536.0 51 782.5 52 1029.0 53 1459.5 54 1561.5 55 1233.0 56 1056.5 57 880.0 58 861.0 59 842.0 60 704.5 61 567.0 62 503.5 63 359.5 64 279.0 65 216.5 66 154.0 67 116.0 68 78.0 69 63.0 70 48.0 71 35.0 72 20.0 73 18.0 74 11.5 75 5.0 76 4.0 77 3.0 78 3.5 79 3.0 80 2.0 81 1.5 82 1.0 83 1.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.031131865688236605 26 0.0400266844563042 27 0.08450077829664221 28 0.1067378252168112 29 0.1423171002890816 30 0.20013342228152103 31 0.20902824104958861 32 0.2401601067378252 33 0.2801867911941294 34 0.2801867911941294 35 0.32466088503446744 36 0.3602401601067379 37 0.38692461641094067 38 0.4358461196353124 39 0.46253057593951524 40 0.5203468979319547 41 0.5114520791638871 42 0.6315321325327997 43 0.6359795419168335 44 0.6315321325327997 45 0.6893484545252391 46 0.7026906826773405 47 0.7293751389815433 48 0.7649544140538136 49 0.7026906826773405 50 0.7516121859017123 51 0.7649544140538136 52 0.8094285078941517 53 0.8272181454302868 54 0.8583500111185234 55 0.8272181454302868 56 0.8183233266622193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 22485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.22592839670892 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45405773652388 39.928841449855454 2 11.768713275994209 12.292639537469425 3 4.470748531039768 7.004669779853236 4 2.4865877544068806 5.194574160551479 5 1.2432938772034403 3.246608850344674 6 0.8515711487694797 2.66844563042028 7 0.4853955547986034 1.7745163442294862 8 0.46836413182321385 1.9568601289748724 9 0.34914417099548667 1.6410940627084722 >10 1.3369667035680832 10.86057371581054 >50 0.017031422975389593 0.5247943073159884 >100 0.05109426892616878 5.368023126528796 >500 0.017031422975389593 7.538358905937291 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 905 4.024905492550589 No Hit CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 790 3.5134534133867024 No Hit CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 476 2.116966866800089 No Hit CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 180 0.8005336891260841 No Hit CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.7649544140538136 No Hit CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.6404269513008672 No Hit CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 129 0.5737158105403602 No Hit CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAA 106 0.4714253947075828 No Hit CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 67 0.29797642873026464 No Hit CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 51 0.22681787858572383 No Hit CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.2179230598176562 No Hit CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.20458083166555482 No Hit CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGT 45 0.20013342228152103 No Hit CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGG 44 0.1956860128974872 No Hit CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.1956860128974872 No Hit CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 40 0.177896375361352 No Hit CGAGTAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.1601067378252168 No Hit CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.1467645096731154 No Hit CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG 32 0.1423171002890816 No Hit CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGGAGCAGG 30 0.13342228152101399 No Hit CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.1289748721369802 No Hit CGAGTAGGGCAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.12452746275294642 No Hit CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.11563264398487881 No Hit CGAGTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.1067378252168112 No Hit CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.10229041583277741 No Hit CGAGTAGGGGTATAGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAG 23 0.10229041583277741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.008894818768067601 0.0 0.0 0.0 0.0 11 0.022237046920169 0.0 0.0 0.0 0.0 12 0.0266844563042028 0.0 0.0 0.0 0.0 13 0.0266844563042028 0.0 0.0 0.0 0.0 14 0.0400266844563042 0.0 0.0 0.0 0.0 15 0.0400266844563042 0.0 0.0 0.0 0.0 16 0.0400266844563042 0.0 0.0 0.0 0.0 17 0.044474093840338 0.0 0.0 0.0 0.0 18 0.048921503224371804 0.0 0.0 0.0 0.0 19 0.0533689126084056 0.0 0.0 0.0 0.0 20 0.066711140760507 0.0 0.0 0.0 0.0 21 0.07115855014454081 0.0 0.0 0.0 0.0 22 0.07115855014454081 0.0 0.0 0.0 0.0 23 0.08450077829664221 0.0 0.0 0.0 0.0 24 0.11563264398487881 0.0 0.0 0.0 0.0 25 0.1245274627529464 0.0 0.0 0.0 0.0 26 0.1245274627529464 0.0 0.0 0.0 0.0 27 0.1245274627529464 0.0 0.0 0.0 0.0 28 0.1645541472092506 0.0 0.0 0.0 0.0 29 0.1645541472092506 0.0 0.0 0.0 0.0 30 0.1867911941294196 0.0 0.0 0.0 0.0 31 0.1867911941294196 0.0 0.0 0.0 0.0 32 0.1867911941294196 0.0 0.0 0.0 0.0 33 0.1867911941294196 0.0 0.0 0.0 0.0 34 0.1867911941294196 0.0 0.0 0.0 0.0 35 0.24016010673782523 0.0 0.0 0.0 0.0 36 0.2623971536579942 0.0 0.0 0.0 0.0 37 0.266844563042028 0.0 0.0 0.0 0.0 38 0.27129197242606184 0.0 0.0 0.0 0.0 39 0.2757393818100956 0.0 0.0 0.0 0.0 40 0.28463420057816324 0.0 0.0 0.0 0.0 41 0.29797642873026464 0.0 0.0 0.0 0.0 42 0.29797642873026464 0.0 0.0 0.0 0.0 43 0.3024238381142984 0.0 0.0 0.0 0.0 44 0.3157660662663998 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCGA 50 5.456968E-12 50.170406 50 TGGCAGC 15 0.008212507 50.170403 40 CAGCATT 15 0.008212507 50.170403 43 CGCCTGG 15 0.008212507 50.170403 36 TAACGGC 15 0.008212507 50.170403 37 CATTTGA 15 0.008212507 50.170403 46 GCAGCAT 15 0.008212507 50.170403 42 TTGATAT 20 4.0273907E-4 50.170403 49 AGCATTT 15 0.008212507 50.170403 44 GCATTTG 15 0.008212507 50.170403 45 CCTGGCA 15 0.008212507 50.170403 38 GAGTGGA 15 0.008285774 50.058167 29 AGGGGTA 30 1.0253643E-6 49.83519 6 AGGGGCT 20 4.163446E-4 49.83519 6 AGGGGAC 15 0.008433764 49.83519 6 AAGAGTG 15 0.008433764 49.83519 10 AGGGCTA 20 4.163446E-4 49.83519 6 AGGGTGT 30 1.0253643E-6 49.83519 6 AGGGTCA 15 0.008433764 49.83519 6 GAAGAGT 15 0.008433764 49.83519 9 >>END_MODULE