FastQCFastQC Report
Tue 31 May 2016
SRR212947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20323
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7303.5919893716478866No Hit
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA7263.5723072381046106No Hit
CGAGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4752.337253358264036No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.8266496088175957No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1450.7134773409437584No Hit
CGAGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.6790336072430251No Hit
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1220.6003050730699208No Hit
CGAGTAGGGGGGAATCCTTCGGCAACATTAACAGGTTGATGCTGAAAAACATCGAA1040.5117354721251783No Hit
CGAGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC540.2657088028342272No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.24602666929095116No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.23126506913349407No Hit
CGAGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG420.20666240220439894No Hit
CGAGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA340.16729813511784677No Hit
CGAGTAGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT330.16237760173202775No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.15745706834620873No Hit
CGAGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1476160015745707No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGT290.14269546818875165No Hit
CGAGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.1328544014171136No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG270.1328544014171136No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT260.12793386803129458No Hit
CGAGTAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11809280125965654No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGG230.11317226787383754No Hit
CGAGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA230.11317226787383754No Hit
CGAGTAGGGCAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1082517344880185No Hit
CGAGTAGGGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.10333120110219947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA203.8888372E-450.50125550
GAGGAGG150.00806928250.37542
AGGAGGG150.00806928250.37543
CCTGGCG150.00806928250.37538
GCTCGCC150.00814961650.24937433
AGGTTGA150.00814961650.24937433
GAGTGCG150.00823054450.1243829
CAGAGGG309.800096E-750.1243827
ATTAACA150.00823054450.1243827
GGCTCGC150.00823054450.1243832
TAACAGG150.00823054450.1243829
CAGGTTG150.00823054450.1243832
AACAGGT150.00823054450.1243830
ACAGGTT150.00823054450.1243831
AGAGGGC251.9851064E-550.12437428
ATCCCCA252.0146803E-550.024
GATCCCC252.0146803E-550.023
GCAGAGG402.4629117E-950.026
TCCCCAA252.0146803E-550.025
AGATCCC252.0446181E-549.87623622
Produced by FastQC (version 0.11.5)