SRR212946_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212946_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41203
Sequences flagged as poor quality	0
Sequence length	58
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2225	5.400092226294202	No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1433	3.4779020945076815	No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1047	2.541077106035968	No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	497	1.2062228478508847	No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	431	1.0460403368686746	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG	358	0.8688687716913817	No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	224	0.5436497342426522	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	206	0.49996359488386766	Illumina Single End Adapter 2 (96% over 25bp)
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.3956022619712157	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	161	0.39074824648690626	No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	141	0.34220809164381233	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG	140	0.3397810839016577	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	136	0.33007305293303885	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGGGG	112	0.2718248671213261	No Hit
CGCACCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	106	0.25726282066839795	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGGGG	105	0.25483581292624324	No Hit
CGCACCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	103	0.24998179744193383	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	95	0.23056573550469628	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	86	0.208722665825304	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	83	0.2014416425988399	Illumina Single End Adapter 2 (100% over 31bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG	82	0.1990146348566852	Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	75	0.18202558066160232	No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75	0.18202558066160232	No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75	0.18202558066160232	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	74	0.1795985729194476	Illumina Single End Adapter 2 (96% over 32bp)
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	0.14804747227143655	No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.14562046452928185	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT	59	0.14319345678712714	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG	58	0.14076644904497246	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	57	0.13833944130281778	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG	55	0.13348542581850836	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT	52	0.12620440259204427	Illumina Single End Adapter 2 (100% over 24bp)
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.12377739484988957	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCG	50	0.12135038710773488	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC	49	0.11892337936558017	No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTAGATGCTGAAAAACATCGAACT	44	0.10678834065480669	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGAACGGAAGAGCTCGTATGCCGTCTTCTGCT	44	0.10678834065480669	Illumina Single End Adapter 1 (96% over 31bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT	43	0.104361332912652	Illumina Single End Adapter 2 (100% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAGAC	60	0.0	52.048603	52
ACTGAGT	60	0.0	52.048603	45
CGCCCCG	20	3.3930197E-4	52.048603	52
GTACTGA	55	0.0	52.048603	43
TCGAACT	35	3.7343852E-8	52.048603	52
CGCTCCG	80	0.0	52.048603	52
ACGTACT	50	3.6379788E-12	52.048603	41
GAGCCGG	15	0.0071445834	52.048603	50
TCGGAGA	60	0.0	52.048603	51
GCCGGGG	35	3.7343852E-8	52.048603	52
CGTACTG	55	0.0	52.048603	42
TACTGAG	55	0.0	52.048603	44
AGCCGGG	20	3.3930197E-4	52.048603	51
GTCGGAG	55	0.0	52.048603	50
ATCCCCT	15	0.0071790335	51.98544	13
AGGGGAG	15	0.0071790335	51.98544	30
GTGCATG	15	0.0071790335	51.98544	23
CCTATCC	15	0.0071790335	51.98544	10
GACAGTG	60	0.0	51.98544	9
GAGTGGG	15	0.0071790335	51.98544	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content