FastQCFastQC Report
Tue 31 May 2016
SRR212945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42270
Sequences flagged as poor quality0
Sequence length58
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT23185.4837946534185No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13803.2647267565649396No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9912.3444523302578664No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5281.249112845990064No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4000.9462976105985333No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2850.674237047551455No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.6198249349420393No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2100.49680624556423Illumina Single End Adapter 2 (96% over 25bp)
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.397444996451384No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1530.36195883605393897No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1330.3146439555240123No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1230.290986515259049No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1070.25313461083510763Illumina Single End Adapter 2 (96% over 32bp)
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.25313461083510763No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1050.248403122782115No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.22474568251715163No Hit
CGCACCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA950.22474568251715163No Hit
CGCACCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT910.21528270641116629No Hit
CGCACCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT850.20108824225218833No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGGGG830.19635675419919563No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC800.18925952211970665No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGGGG770.18216229004021764No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG730.1726993139342323Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC710.16796782588123965No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT710.16796782588123965No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA680.16087059380175064No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.1585048497752543No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC660.156139105748758Illumina Single End Adapter 2 (100% over 31bp)
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.156139105748758No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT590.13957889756328365Illumina Single End Adapter 2 (100% over 24bp)
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCCG540.127750177430802No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT530.12538443340430566Illumina Single End Adapter 2 (100% over 24bp)
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.12301868937780933No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCG510.12065294535131299No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG480.113555713271824No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.113555713271824No Hit
CGCACCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11118996924532765No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG450.10645848119233499No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCT430.10172699313934232Illumina Single End Adapter 2 (100% over 29bp)
CGCACCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA430.10172699313934232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAT150.00711429952.10676224
CAGAGGG600.052.10676227
CCATATT150.00711429952.10676225
ACCCATA150.00711429952.10676223
AAAGCAG203.3753112E-452.10676223
CATATTA150.00711429952.10676226
ACGCGGA150.00711429952.10676224
ATATTAG150.00711429952.10676227
GGAGATG203.4151197E-451.9834338
TGGTTTG150.007181391651.98343350
GGATGTA251.631059E-551.9834338
AGGGGAG150.007181391651.98343330
CAGTTTT150.007181391651.98343334
AGCTTTT150.007181391651.98343317
GACAGTG401.8262654E-951.9834339
AAATTCG150.007181391651.98343348
CGGGGTT251.631059E-551.9834336
GAGTGCC150.007181391651.98343329
CGGGGTA307.8290213E-751.9834336
TTCGTAT150.007181391651.98343351
Produced by FastQC (version 0.11.5)