SRR212944_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212944_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44325
Sequences flagged as poor quality	0
Sequence length	58
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2203	4.970107163000564	No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1318	2.9734912577552173	No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	937	2.113931190073322	No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	511	1.1528482797518331	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG	432	0.9746192893401016	No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	377	0.85053581500282	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	195	0.4399323181049069	Illumina Single End Adapter 2 (96% over 25bp)
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	182	0.4106034968979131	No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.3496897913141568	No Hit
CGCACCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	134	0.30231246474901297	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG	133	0.30005640157924424	No Hit
CGCACCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	129	0.2910321489001692	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	123	0.27749576988155666	No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	121	0.2729836435420192	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGGGG	121	0.2729836435420192	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	112	0.2526790750141004	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGGGG	109	0.24591088550479415	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	100	0.2256063169768754	No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.20078962210941906	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	72	0.16243654822335024	Illumina Single End Adapter 2 (100% over 31bp)
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.15792442188381275	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	67	0.15115623237450648	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT	63	0.14213197969543148	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG	62	0.13987591652566272	Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.13310772701635645	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	58	0.1308516638465877	Illumina Single End Adapter 2 (96% over 32bp)
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	55	0.12408347433728144	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG	55	0.12408347433728144	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGTGG	55	0.12408347433728144	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	55	0.12408347433728144	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT	53	0.11957134799774395	Illumina Single End Adapter 2 (100% over 24bp)
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.11957134799774395	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT	51	0.11505922165820644	Illumina Single End Adapter 2 (100% over 24bp)
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG	49	0.11054709531866892	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCT	46	0.10377890580936266	Illumina Single End Adapter 2 (100% over 29bp)
CGCACCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT	45	0.10152284263959391	No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.10152284263959391	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCTTC	45	0.10152284263959391	Illumina Single End Adapter 2 (96% over 28bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGTA	30	7.4756645E-7	52.33941	8
GGATGGT	30	7.4756645E-7	52.33941	8
AAGTACA	15	0.0069919988	52.33941	10
GGGTCCC	15	0.0069919988	52.33941	7
CGGGGTT	15	0.0069919988	52.33941	6
CGGGGAC	15	0.0069919988	52.33941	6
GGGTACG	15	0.0069919988	52.33941	7
GAAGTAT	15	0.0069919988	52.33941	9
GAAGTAC	15	0.0069919988	52.33941	9
GAATGTA	15	0.0069919988	52.33941	9
GAGCATG	15	0.0069919988	52.33941	9
GACATGT	15	0.0069919988	52.33941	9
CGGGTCA	15	0.0069919988	52.33941	6
CGGGTAT	30	7.4756645E-7	52.33941	6
CGGGATG	95	0.0	52.33941	6
GGAAGTT	15	0.0069919988	52.33941	8
GGAAGTA	30	7.4756645E-7	52.33941	8
GGGACTG	70	0.0	52.33941	7
GGGATGG	35	3.5812263E-8	52.33941	7
AAGTGTA	15	0.0069919988	52.33941	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content