SRR212943_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212943_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49402
Sequences flagged as poor quality	0
Sequence length	56
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3116	6.307436945872637	No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1146	2.319744139913364	No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	922	1.8663212015707868	No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	435	0.8805311525849155	No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	397	0.8036111898303713	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	271	0.5485607870126715	Illumina Single End Adapter 2 (95% over 23bp)
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	223	0.45139872879640497	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGG	202	0.40889032832678834	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGG	201	0.4068661187806162	No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	191	0.386624023318894	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	123	0.24897777417918301	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	121	0.2449293550868386	No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	113	0.22873567871746084	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	110	0.22266305007894419	Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.21659042144042753	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	100	0.20242095461722198	Illumina Single End Adapter 2 (100% over 29bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	93	0.18825148779401643	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92	0.1862272782478442	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	90	0.18217885915549978	No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.18015464960932756	No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.1761062305169831	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGAGCCGG	86	0.1740820209708109	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC	84	0.17003360187846644	Illumina Single End Adapter 2 (100% over 22bp)
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	81	0.1639609732399498	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAGCAGG	80	0.16193676369377757	No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76	0.1538399255090887	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC	71	0.1437188777782276	Illumina Single End Adapter 2 (96% over 28bp)
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	69	0.13967045868588315	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	69	0.13967045868588315	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGCGCCGG	66	0.1335978300473665	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGCGCCGG	65	0.13157362050119428	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCT	63	0.12752520140884985	Illumina Single End Adapter 2 (96% over 26bp)
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.12145257277033318	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	59	0.11942836322416098	No Hit
CGCACCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.11740415367798875	No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.10323468685478321	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	50	0.10121047730861099	No Hit
CGCACCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG	50	0.10121047730861099	No Hit
CGCACCGGGAAGCATGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	50	0.10121047730861099	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTT	50	0.10121047730861099	Illumina Single End Adapter 2 (100% over 27bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTGTA	15	0.008234771	50.226532	47
GAGCCGG	40	2.4920155E-9	50.226532	50
GCCGCTC	15	0.008234771	50.226532	50
TGTATGG	15	0.008234771	50.226532	50
CAACGGG	15	0.008234771	50.226532	43
GCCCTTT	15	0.008234771	50.226532	45
GGTATGG	25	2.0037764E-5	50.22653	50
CGCCTTC	20	4.073568E-4	50.175335	37
CCCGCTC	20	4.073568E-4	50.175335	39
TGCCCGC	20	4.073568E-4	50.175335	37
CCCCGGC	40	2.5138434E-9	50.175335	37
GTGCCCG	20	4.073568E-4	50.175335	36
CCCGGCA	15	0.008268083	50.17533	38
TGATCGG	15	0.008268083	50.17533	37
GGCTGGC	15	0.008268083	50.17533	37
CGGACCG	15	0.008268083	50.17533	41
TCGGACC	15	0.008268083	50.17533	40
CAGAGCT	65	0.0	50.17533	34
AGTGCTT	15	0.008268083	50.17533	35
AGTGCCC	15	0.008268083	50.17533	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content