FastQCFastQC Report
Tue 31 May 2016
SRR212943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49402
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT31166.307436945872637No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA11462.319744139913364No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9221.8663212015707868No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4350.8805311525849155No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3970.8036111898303713No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG2710.5485607870126715Illumina Single End Adapter 2 (95% over 23bp)
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.45139872879640497No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGG2020.40889032832678834No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGG2010.4068661187806162No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.386624023318894No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1230.24897777417918301No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC1210.2449293550868386No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1130.22873567871746084No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG1100.22266305007894419Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.21659042144042753No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT1000.20242095461722198Illumina Single End Adapter 2 (100% over 29bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA930.18825148779401643No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.1862272782478442No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC900.18217885915549978No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.18015464960932756No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1761062305169831No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGAGCCGG860.1740820209708109No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC840.17003360187846644Illumina Single End Adapter 2 (100% over 22bp)
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC810.1639609732399498No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAGCAGG800.16193676369377757No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1538399255090887No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC710.1437188777782276Illumina Single End Adapter 2 (96% over 28bp)
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG690.13967045868588315No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT690.13967045868588315No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGCGCCGG660.1335978300473665No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGCGCCGG650.13157362050119428No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCT630.12752520140884985Illumina Single End Adapter 2 (96% over 26bp)
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.12145257277033318No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC590.11942836322416098No Hit
CGCACCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.11740415367798875No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10323468685478321No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA500.10121047730861099No Hit
CGCACCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG500.10121047730861099No Hit
CGCACCGGGAAGCATGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT500.10121047730861099No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTT500.10121047730861099Illumina Single End Adapter 2 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA150.00823477150.22653247
GAGCCGG402.4920155E-950.22653250
GCCGCTC150.00823477150.22653250
TGTATGG150.00823477150.22653250
CAACGGG150.00823477150.22653243
GCCCTTT150.00823477150.22653245
GGTATGG252.0037764E-550.2265350
CGCCTTC204.073568E-450.17533537
CCCGCTC204.073568E-450.17533539
TGCCCGC204.073568E-450.17533537
CCCCGGC402.5138434E-950.17533537
GTGCCCG204.073568E-450.17533536
CCCGGCA150.00826808350.1753338
TGATCGG150.00826808350.1753337
GGCTGGC150.00826808350.1753337
CGGACCG150.00826808350.1753341
TCGGACC150.00826808350.1753340
CAGAGCT650.050.1753334
AGTGCTT150.00826808350.1753335
AGTGCCC150.00826808350.1753335