SRR212942_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212942_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45003
Sequences flagged as poor quality	0
Sequence length	56
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	2871	6.379574695020332	No Hit
CGCACCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1145	2.5442748261227033	No Hit
CGCACCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	940	2.088749638912961	No Hit
CGCACCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	438	0.9732684487700819	No Hit
CGCACCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	423	0.9399373375108325	No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	239	0.5310757060640402	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	231	0.5132991133924405	Illumina Single End Adapter 2 (95% over 23bp)
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.39108503877519274	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCCGG	168	0.3733084461035931	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGAGCAGG	123	0.27331511232584493	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	123	0.27331511232584493	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	111	0.24665022331844547	No Hit
CGCACCGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	100	0.22220740839499592	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	98	0.21776326022709597	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.21331911205919604	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	94	0.20887496389129612	Illumina Single End Adapter 2 (100% over 29bp)
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.19554251938759637	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	83	0.1844321489678466	Illumina Single End Adapter 2 (96% over 30bp)
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80	0.17776592671599672	No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.17554385263204675	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCC	78	0.1733217785480968	Illumina Single End Adapter 2 (100% over 22bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGAGCCGG	77	0.17109970446414685	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	70	0.15554518587649713	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTCGCACCGGGGCGCCGG	68	0.1511010377085972	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	65	0.14443481545674733	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	64	0.14221274137279738	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC	61	0.13554651912094748	Illumina Single End Adapter 2 (100% over 22bp)
CGCACCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC	61	0.13554651912094748	Illumina Single End Adapter 2 (96% over 28bp)
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.13554651912094748	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAGCAGG	61	0.13554651912094748	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	58	0.1288802968690976	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGGCGCCGG	57	0.12665822278514766	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	57	0.12665822278514766	No Hit
CGCACCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	54	0.11999200053329777	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCT	53	0.11776992644934783	Illumina Single End Adapter 2 (96% over 26bp)
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	48	0.10665955602959802	No Hit
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.10665955602959802	No Hit
CGCACCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	46	0.10221540786169811	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	46	0.10221540786169811	No Hit
CGCACCGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCCC	46	0.10221540786169811	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTTAT	25	1.9716572E-5	50.351295	49
AAGCAGG	25	1.9716572E-5	50.351295	50
TGCAATG	15	0.008149575	50.35129	49
GATATGG	30	9.768337E-7	50.35129	50
GAAGCCG	15	0.008149575	50.35129	49
GAGCCGG	15	0.008149575	50.35129	50
GGTATGG	35	4.861431E-8	50.35129	50
GGAGCCG	15	0.008149575	50.35129	49
CATCGAA	45	1.2187229E-10	50.351288	50
TTGCAAT	15	0.008222241	50.23852	48
GCGGCCT	20	4.0444062E-4	50.23852	42
TCGTTGC	15	0.008222241	50.23852	45
CGGCCTT	20	4.0444062E-4	50.23852	43
CAGGCCC	15	0.008222241	50.23852	47
CTTTGTT	20	4.0444062E-4	50.23852	47
CGTTGCA	15	0.008222241	50.23852	46
CTCGTTG	15	0.008222241	50.23852	44
TTTGTTA	20	4.0444062E-4	50.23852	48
GTTGCAA	15	0.008222241	50.23852	47
AGTGCTT	25	2.011364E-5	50.18233	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content