FastQCFastQC Report
Tue 31 May 2016
SRR212941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38673
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT18714.838000672303674No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG13183.4080624725260513No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC9462.4461510614640707No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC8032.0763840405450833No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5101.3187495151656194No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4481.1584309466552893No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG4291.1093010627569622No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.736948258474905No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.7266051250226256No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2600.6723036743981589No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2190.5662865565122954No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCAGGCCGCTCCGA1470.3801101543712668No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1320.34132340392521915No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCTCCGA1320.34132340392521915No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1220.3154655702945207No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1160.29995087011610166No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGTGG1150.29736508675303186No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.26892146975926357No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC1020.2637499030331239No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC980.2534067695808445Illumina Single End Adapter 2 (96% over 25bp)
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT950.245649419491635No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGGGG860.2223773692240064No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.2094484524086572No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT730.18876218550409848No Hit
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG710.1835906187779588No Hit
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC660.17066170196260957No Hit
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.17066170196260957No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT560.14480386833191117Illumina Single End Adapter 2 (96% over 32bp)
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC490.12670338479042226Illumina Single End Adapter 2 (100% over 31bp)
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.11894603470121275No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC450.11636025133814289No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCG450.11636025133814289No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC430.1111886846120032No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT420.10860290124893336No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC410.10601711788586353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT401.7953425E-952.06347352
TGATCAT150.00713317952.06347351
TTGATCA150.00713317952.06347350
GCTCCGA503.6379788E-1252.06347352
TGGCCAA203.3855956E-452.06347351
TATGGGT203.3855956E-452.06347352
TATGGAT458.731149E-1152.0634752
GGAGATT203.4073554E-451.996128
ACAGTGG203.4073554E-451.9961210
AACGCGG251.6260898E-551.9961223
GTGTCTC203.4073554E-451.9961225
GTTTGAT203.4073554E-451.9961248
TGCGTGT203.4073554E-451.9961222
TGTCTCC203.4073554E-451.9961226
GTCAAAC203.4073554E-451.996129
TGCGATC203.4073554E-451.9961232
GAACGCA203.4073554E-451.996129
AGGGTCA503.6379788E-1251.996126
TGGAGTG251.6260898E-551.9961232
GGTCAAA203.4073554E-451.996128
Produced by FastQC (version 0.11.5)