SRR212940_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212940_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37931
Sequences flagged as poor quality	0
Sequence length	58
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	1831	4.827186206532915	No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1340	3.53273048430044	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	898	2.367456697687907	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	549	1.4473649521499565	No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	473	1.2470011336373943	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	467	1.231182937439034	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG	388	1.0229100208272917	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	315	0.8304553004139095	No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	260	0.6854551685956078	No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	256	0.6749097044633677	No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	215	0.5668186971079064	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCAGGCCGCTCCGA	137	0.36118214652922415	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCTCCGA	131	0.3453639503308639	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC	130	0.3427275842978039	No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.3242730220663837	No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	109	0.28736389760354325	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG	105	0.27681843347130314	No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95	0.25045477314070286	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGTGG	95	0.25045477314070286	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC	86	0.22672747884316258	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	86	0.22672747884316258	Illumina Single End Adapter 2 (96% over 25bp)
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	76	0.2003638185125623	No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT	72	0.18981835438032216	No Hit
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	71	0.18718198834726213	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	69	0.18190925628114207	Illumina Single End Adapter 2 (96% over 32bp)
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGGGG	63	0.1660910600827819	No Hit
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.15554559595054177	No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	54	0.14236376578524162	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCG	50	0.1318183016530015	No Hit
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.12654556958688143	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	46	0.12127283752076139	Illumina Single End Adapter 2 (100% over 31bp)
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC	45	0.11863647148770136	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC	44	0.11600010545464133	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT	44	0.11600010545464133	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.11072737338852126	No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG	42	0.11072737338852126	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCAA	20	3.347864E-4	52.17881	24
TGCATGT	20	3.347864E-4	52.17881	24
ATGTGGT	20	3.347864E-4	52.17881	27
GATCCCA	20	3.347864E-4	52.17881	23
GCATGTG	20	3.347864E-4	52.17881	25
ACGCGAT	20	3.347864E-4	52.17881	24
CATGTGG	20	3.347864E-4	52.17881	26
CAACGCG	40	1.7589628E-9	52.17881	22
CGCGATC	20	3.347864E-4	52.17881	25
AACGCGA	30	7.6070137E-7	52.178806	23
AACGCCG	25	1.5919673E-5	52.178806	23
ACTGAGT	15	0.0070698294	52.178806	23
GTACTGA	15	0.0070698294	52.178806	21
CAGAGGG	30	7.6070137E-7	52.178806	27
TACTGAG	15	0.0070698294	52.178806	22
CAACGCC	25	1.5919673E-5	52.178806	22
TGTTGGG	15	0.007181785	51.972298	37
TGGTCGA	20	3.414223E-4	51.972298	34
AGGGGCA	55	0.0	51.972298	6
GGAGACA	20	3.414223E-4	51.972298	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content