FastQCFastQC Report
Tue 31 May 2016
SRR212939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38608
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT17604.5586406962287604No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG11723.0356402818068795No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8162.113551595524244No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC7541.9529631164525485No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4111.0645462080397845No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC3881.004973062577704No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3660.9479900538748446No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.7174678823041857No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2420.6268130957314546No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.572420223787816No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2030.52579776212184No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCAGGCCGCTCCGA1790.46363447990053874No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGTGG1390.3600290095317033No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1320.3418980522171571No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCTCCGA1080.2797347699958558No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC950.24606299212598426Illumina Single End Adapter 2 (96% over 25bp)
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG920.23829258184832158No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT890.2305221715706589No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.22534189805221713No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC810.20980107749689184No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC790.20462080397845006No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.2020306672192292No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGGGG690.1787194363862412No Hit
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC680.1761292996270203No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT630.16317861583091586No Hit
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG570.14763779527559054No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT530.13727724823870702Illumina Single End Adapter 2 (96% over 32bp)
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.13209697472026524No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC470.12173642768338168No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCG440.11396601740571902No Hit
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.11137588064649814No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC430.11137588064649814Illumina Single End Adapter 2 (100% over 31bp)
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC410.10619560712805637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGG150.00703363752.24804310
AGGGGGT353.614514E-852.2480436
AGGGGCG307.540657E-752.2480436
AGGGGAT203.3266353E-452.2480436
GGTGACA203.3266353E-452.2480439
GAAGCTG353.614514E-852.2480439
GAAGTGT150.00703363752.2480439
AAGAGTG203.3266353E-452.24804310
GAAGTGG307.540657E-752.2480439
AGGGTTC203.3266353E-452.2480436
AGGGTGA150.00703363752.2480436
AGGGTAC150.00703363752.2480436
GGCTGTA203.3266353E-452.2480438
AGGGACG203.3266353E-452.2480436
AGGGACC150.00703363752.2480436
GGGAGCT307.540657E-752.2480437
GGATACA150.00703363752.2480438
GGGGGTT150.00703363752.2480437
AAGATGG150.00703363752.24804310
GCTGTAC150.00703363752.2480439
Produced by FastQC (version 0.11.5)