SRR212938_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212938_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34756
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1851	5.325699159857291	No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1060	3.049833122338589	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	671	1.9306019104615029	No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	384	1.104845206583036	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	298	0.8574059155253768	No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.7912302911727471	No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	236	0.6790194498791575	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	211	0.6070894234089078	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	201	0.578317412820808	No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	196	0.563931407526758	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	137	0.39417654505696853	No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.30210611117504893	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	102	0.29347450799861896	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGG	100	0.28772010588099894	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGT	95	0.273334100586949	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	91	0.26182529635170904	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	83	0.23880768788122914	Illumina Single End Adapter 2 (95% over 23bp)
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC	75	0.21579007941074924	No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.20428127517550929	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	70	0.20140407411669928	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCC	67	0.1927724709402693	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCAGGCCGCCCC	63	0.18126366670502936	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCT	61	0.17550926458740937	No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG	59	0.16975486246978938	No Hit
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.15824605823454943	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCCCC	54	0.15536885717573945	No Hit
GGGCGAGGGGGGGTGTCCATGTATTGAAACTAAGCCCATAAACACGTTTGATTTGT	50	0.14386005294049947	No Hit
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.12947404764644954	No Hit
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	45	0.12947404764644954	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	42	0.12084244447001956	Illumina Single End Adapter 2 (96% over 30bp)
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	41	0.11796524341120956	No Hit
GGGCGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.11221084129358959	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	39	0.11221084129358959	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCCC	37	0.10645643917596961	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTC	36	0.10357923811715962	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA	35	0.10070203705834964	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGAA	35	4.9456503E-8	50.185238	50
GCCCCCC	25	2.0010752E-5	50.185238	50
TTTCCCC	15	0.008241063	50.185234	50
TGCGATC	35	5.0025847E-8	50.11272	32
GTGCGAT	35	5.0025847E-8	50.11272	31
CGTTTGA	15	0.008288404	50.112717	45
GTTTGCT	15	0.008288404	50.112717	32
GTGCAGA	20	4.08184E-4	50.112717	31
AGAAGTC	15	0.008288404	50.112717	41
CGCCCGG	15	0.008288404	50.112717	36
TCCTAAG	15	0.008288404	50.112717	46
CTGGGAG	15	0.008288404	50.112717	39
CCTAAGA	15	0.008288404	50.112717	47
GGCAAAC	15	0.008288404	50.112717	36
GTTCTAA	15	0.008288404	50.112717	35
AGAGGGA	15	0.008288404	50.112717	44
TAGTTCT	15	0.008288404	50.112717	33
TGGAGCG	15	0.008288404	50.112717	32
GAGTTTT	15	0.008288404	50.112717	34
AGCAGGC	25	2.0183283E-5	50.112717	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content