##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34756 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.3950684773852 38.0 37.0 40.0 33.0 40.0 2 36.91046150304983 38.0 36.0 39.0 32.0 40.0 3 36.69156404649557 38.0 35.0 39.0 31.0 40.0 4 36.36114627690183 38.0 35.0 39.0 31.0 40.0 5 37.13252388076879 38.0 36.0 39.0 33.0 40.0 6 35.52485901714812 38.0 35.0 39.0 29.0 40.0 7 37.46570376337898 39.0 38.0 40.0 34.0 40.0 8 37.2531361491541 39.0 38.0 40.0 33.0 40.0 9 37.1852054321556 39.0 38.0 40.0 33.0 40.0 10 35.291892047416276 38.0 34.0 39.0 28.0 40.0 11 35.20318793877316 38.0 35.0 39.0 28.0 40.0 12 35.374554033835885 38.0 35.0 39.0 28.0 40.0 13 35.05958683392795 38.0 34.0 39.0 28.0 40.0 14 35.03921625043158 38.0 34.0 39.0 28.0 40.0 15 35.421164690988604 38.0 35.0 39.0 29.0 40.0 16 35.11209575325124 38.0 35.0 39.0 28.0 40.0 17 35.43333525146737 38.0 35.0 39.0 29.0 40.0 18 35.24438945793532 38.0 35.0 39.0 29.0 40.0 19 34.27885832661986 38.0 33.0 39.0 26.0 40.0 20 34.834877431234894 38.0 35.0 39.0 27.0 40.0 21 34.675883300725054 38.0 35.0 39.0 27.0 40.0 22 34.21719990792957 38.0 33.0 39.0 26.0 40.0 23 33.85596731499597 38.0 33.0 39.0 24.0 40.0 24 33.81234894694441 38.0 33.0 39.0 24.0 40.0 25 33.85720451145126 38.0 33.0 39.0 24.0 40.0 26 33.31493842789734 38.0 33.0 39.0 21.0 40.0 27 33.246777534814136 38.0 33.0 39.0 20.0 40.0 28 32.34025779721487 36.0 32.0 39.0 15.0 40.0 29 32.443491771204975 37.0 32.0 39.0 14.0 40.0 30 31.879445275635863 36.0 31.0 39.0 2.0 40.0 31 30.408217286223962 36.0 30.0 39.0 2.0 40.0 32 29.01335021291288 35.0 24.0 39.0 2.0 40.0 33 29.8322016342502 35.0 29.0 39.0 2.0 40.0 34 29.919208194268617 36.0 29.0 39.0 2.0 40.0 35 28.247640695131775 35.0 23.0 39.0 2.0 40.0 36 27.279232362757508 35.0 4.0 39.0 2.0 40.0 37 26.698354240994362 35.0 2.0 39.0 2.0 40.0 38 26.626222810449995 35.0 2.0 39.0 2.0 40.0 39 26.672200483369778 35.0 2.0 39.0 2.0 40.0 40 26.301358038899757 35.0 2.0 39.0 2.0 40.0 41 26.182385775117964 35.0 2.0 39.0 2.0 40.0 42 26.16474853262746 35.0 2.0 39.0 2.0 40.0 43 25.956813212107264 35.0 2.0 39.0 2.0 40.0 44 25.601421337323053 35.0 2.0 39.0 2.0 40.0 45 25.589135688801935 35.0 2.0 39.0 2.0 40.0 46 25.31059385429854 35.0 2.0 39.0 2.0 40.0 47 25.205834963747268 35.0 2.0 39.0 2.0 40.0 48 25.07210265853378 35.0 2.0 39.0 2.0 40.0 49 24.949533893428473 35.0 2.0 39.0 2.0 40.0 50 24.649988491195764 35.0 2.0 39.0 2.0 40.0 51 24.231010473011853 35.0 2.0 39.0 2.0 40.0 52 23.969587984808378 34.0 2.0 39.0 2.0 40.0 53 23.554005063873863 34.0 2.0 39.0 2.0 40.0 54 23.28970537461158 33.0 2.0 39.0 2.0 40.0 55 22.86767752330533 33.0 2.0 39.0 2.0 40.0 56 13.008430199102314 16.0 2.0 22.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.056116629328151646 1 2 -0.8020659631551084 1 3 -1.0242386328687303 1 4 -1.3582251478061806 1 5 0.5920656691419381 1 6 0.5605288783921836 1 7 -0.5627472919906893 1 8 -0.38902904884237444 1 9 -0.6005511547616962 1 10 -1.6152693160188036 1 11 -1.167627130627075 1 12 -1.1396382543646624 1 13 -1.2757079050668878 1 14 0.2417771988736419 1 15 -1.0223983999289175 1 16 -4.019907017009729 1 17 -3.3679474794533846 1 18 -3.947740529372826 1 19 -3.0873379912589805 1 20 -2.33191386060021 1 21 -2.0247737357257094 1 22 -2.566247440566766 1 23 -2.578548978223303 1 24 -2.55752738447552 1 25 -3.865074407919238 1 26 -1.6059870795471127 1 27 -4.144161926151273 1 28 -2.117884181608808 1 29 -2.6644320655372873 1 30 -2.0805055579093157 1 31 -5.546218680399122 1 32 -3.769067194147855 1 33 -5.073992063006649 1 34 -4.689976450559069 1 35 -4.788739429825839 1 36 -3.471646618738699 1 37 -5.053218389157884 1 38 -5.142825251975825 1 39 -4.35013927114899 1 40 -4.677544452937038 1 41 -3.3848565378781252 1 42 -2.951782521104377 1 43 -4.762194625990219 1 44 -2.4302072739389615 1 45 -3.316418602536544 1 46 -4.604200306714176 1 47 -2.9391108134825217 1 48 -3.7633816668477564 1 49 -3.750123885080953 1 50 -4.728113335282984 1 51 -3.2206585163181174 1 52 -3.406642587883386 1 53 -2.740064142830846 1 54 -2.105146747800699 1 55 -5.51354735600837 1 56 -1.9964756897575011 2 1 -0.28871095687874515 2 2 -1.3882728597068308 2 3 -0.43803173631700076 2 4 0.5728093349524386 2 5 -0.6148308825822042 2 6 0.25018405080597717 2 7 -0.2179197057837996 2 8 0.024764054605896035 2 9 0.1580695348934782 2 10 -0.16699345394983567 2 11 -1.0986616133856941 2 12 -1.6223968750543207 2 13 -2.9308803188599875 2 14 -2.1720159045746357 2 15 -1.9534328826875367 2 16 -1.7095621894235222 2 17 -1.3679474794533846 2 18 -3.3270508742004132 2 19 -2.2942345429831192 2 20 -2.262948343358829 2 21 -1.0592564943463998 2 22 -1.1179715784978015 2 23 -1.7164800127060644 2 24 -1.350630832751385 2 25 -1.520246821712341 2 26 0.049185334245990475 2 27 -0.9027826158064443 2 28 0.08901237011533425 2 29 -0.4920182724338389 2 30 -0.011540040667934903 2 31 1.419298560980188 2 32 1.4723121161969743 2 33 -0.14295758024803007 2 34 0.8272649287512728 2 35 1.625053673622439 2 36 3.321456829537162 2 37 2.9812643694628065 2 38 1.2020023342310715 2 39 0.1671021081613553 2 40 1.977627960856065 2 41 1.4427296690184264 2 42 1.7378726513094165 2 43 2.651598477458055 2 44 2.5008272088196613 2 45 2.683581397463456 2 46 2.2233859001823753 2 47 2.7850271175519623 2 48 2.788342471083279 2 49 3.3533243907811148 2 50 3.927059078510119 2 51 3.4000311388542954 2 52 4.593357412116614 2 53 4.604763443376051 2 54 5.101749803923436 2 55 4.2795560922674944 2 56 2.1069725861045665 3 1 -0.12204429021208085 3 2 -0.003215388442463052 3 3 -0.14148001217907336 3 4 0.2866024384007133 3 5 0.5437898070729688 3 6 -0.1728044549411507 3 7 -0.357000165553913 3 8 -0.1476497384975488 3 9 0.3155407992612922 3 10 -0.3014762125705275 3 11 -1.6262478202822521 3 12 -0.5419371049393789 3 13 -1.1607653763312555 3 14 -0.8237400425056691 3 15 -1.0315938022277678 3 16 -2.3532403503430643 3 17 -1.9369129966947654 3 18 -2.589119839717654 3 19 -1.6942345429831178 3 20 -2.116971331864576 3 21 -3.6477622414728366 3 22 -2.750155486543779 3 23 -3.291192656384222 3 24 -1.934538878728393 3 25 -4.799557166539927 3 26 -2.227826160006881 3 27 -3.3728975583351826 3 28 -2.0937462505743234 3 29 -2.1586849391005067 3 30 -2.6402756728518426 3 31 0.45148246902616407 3 32 -0.21734305621681926 3 33 0.8237090864186349 3 34 0.6628971126593193 3 35 0.7917203402891069 3 36 0.393870622640609 3 37 0.5847126453248741 3 38 1.1008529089437147 3 39 1.130320498965954 3 40 1.7293520987870998 3 41 1.046177944880494 3 42 1.230976099585277 3 43 1.6205639946994346 3 44 1.754850197325407 3 45 1.89507565033702 3 46 2.293500842711108 3 47 2.2746822899657566 3 48 2.817078103267189 3 49 3.005048528712152 3 50 1.6374039060963241 3 51 1.3448587250611936 3 52 1.3772654580936248 3 53 2.1334990755599605 3 54 -0.24652605814552686 3 55 0.23817678192266456 3 56 1.6357082182884746 4 1 -0.19496095687874515 4 2 0.13220127822420125 4 3 -0.8539800121790719 4 4 0.5803524384007162 4 5 0.7062898070729702 4 6 1.0605288783921836 4 7 0.45341650111275555 4 8 0.6961002615024512 4 9 0.2884574659279622 4 10 0.04852378742947394 4 11 -1.3637478202822493 4 12 -0.6461037716060432 4 13 -0.8899320429979198 4 14 -0.9112400425056677 4 15 -0.7982604688944335 4 16 0.7300929829902714 4 17 0.7818370033052346 4 18 0.09213016028234478 4 19 -0.16923454298311924 4 20 0.9913620014687581 4 21 1.035571091860497 4 22 1.572761180122889 4 23 1.47964067694911 4 24 2.2883777879382734 4 25 0.7566928334600718 4 26 2.457590506659784 4 27 3.8375191083314846 4 28 1.106253749425676 4 29 3.414231727566161 4 30 2.532640993814823 4 31 0.5410658023594976 4 32 -0.49859305621681926 4 33 -0.29920758024803007 4 34 0.5837304459926536 4 35 -0.562446326377561 4 36 0.5876206226406104 4 37 0.8659626453248741 4 38 0.7612695756103811 4 39 0.6574038322992877 4 40 0.37310209878710054 4 41 -0.266322055119506 4 42 0.6247260995852777 4 43 0.345563994699436 4 44 0.43401686399207406 4 45 -0.06534101632964706 4 46 -0.39191582395555713 4 47 -0.4940677100342441 4 48 0.6191614366005211 4 49 0.030048528712150357 4 50 0.20615390609632556 4 51 1.0573587250611922 4 52 1.079348791426959 4 53 1.3730824088932927 4 54 0.6222239418544717 4 55 0.38192678192266527 4 56 0.6877915516218085 5 1 1.0237890431212548 5 2 0.5072012782242012 5 3 0.8647699878209281 5 4 1.6428524384007162 5 5 1.1750398070729702 5 6 1.5292788783921836 5 7 0.5159165011127556 5 8 1.0711002615024512 5 9 0.6947074659279622 5 10 0.45477378742947394 5 11 -0.11374782028224928 5 12 0.8226462283939568 5 13 0.17256795700208016 5 14 -0.2862400425056677 5 15 -0.3295104688944335 5 16 0.48009298299027137 5 17 0.9068370033052346 5 18 1.0921301602823448 5 19 -0.5442345429831192 5 20 0.7413620014687581 5 21 1.473071091860497 5 22 1.666511180122889 5 23 -0.27035932305089005 5 24 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1.7025688980204805 8 21 1.2510883332398066 8 22 1.2268560077090953 8 23 1.6283475735008324 8 24 2.270058822421035 8 25 1.6176842127704134 8 26 2.3250474032115065 8 27 2.8903208324694134 8 28 2.5028054735636047 8 29 3.3700506930833996 8 30 3.5401840972631007 8 31 3.9365399402905297 8 32 2.299898323093526 8 33 0.9604906956140375 8 34 1.9651959632340343 8 35 2.5216053977603714 8 36 2.183525795054404 8 37 3.4985057487731517 8 38 2.6157954376793455 8 39 2.994688315057907 8 40 4.115558995338823 8 41 2.7185917379839424 8 42 1.703389892688726 8 43 2.0309088222856424 8 44 1.983585829509316 8 45 1.6146158802220754 8 46 2.3957996932858237 8 47 2.1643374623795495 8 48 1.8917907469453503 8 49 0.8016002528500827 8 50 2.0305073543721868 8 51 3.0207207940267082 8 52 1.8002539638407526 8 53 1.501315167513983 8 54 1.7914049763372297 8 55 0.17610781640542328 8 56 1.7621449998976697 9 1 -0.15967869881422558 9 2 -1.2559035604854785 9 3 -0.315673560566168 9 4 -2.271462077728316 9 5 -0.04975858002380562 9 6 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-4.902081613701085 120 55 -3.998860255114373 120 56 -2.1791065965263403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86.0 3 18.0 4 57.0 5 30.0 6 106.0 7 312.0 8 127.0 9 163.0 10 338.0 11 209.0 12 249.0 13 285.0 14 297.0 15 313.0 16 410.0 17 434.0 18 544.0 19 653.0 20 797.0 21 909.0 22 848.0 23 1088.0 24 1170.0 25 741.0 26 552.0 27 497.0 28 549.0 29 672.0 30 800.0 31 908.0 32 1251.0 33 1489.0 34 1847.0 35 2135.0 36 2845.0 37 4293.0 38 4239.0 39 2495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 40.13120036828173 45.813672459431466 5.62205086891472 8.43307630337208 11 35.064449303717346 40.48797329957418 13.482564161583612 10.965013235124871 12 61.31027736218206 18.267349522384624 11.19806652088848 9.224306594544828 13 17.26320635285994 31.404649556911036 18.342156749913684 32.98998734031534 14 18.696052480147312 50.10933364023478 19.51605478190816 11.678559097709748 15 41.221659569570726 14.621935780872366 33.71216480607665 10.444239843480263 16 14.4579353205202 15.772816204396364 48.91529520082864 20.853953274254806 17 36.2613649441823 15.326850040280815 13.445160547819082 34.96662446771781 18 35.57946829324433 18.44285878697203 20.209460237081366 25.76821268270227 19 18.172401887443897 17.487628035447116 50.12371964552883 14.216250431580157 20 27.97502589480953 34.327885832661984 20.324548279433767 17.37253999309472 21 26.985268730578895 16.9639774427437 33.956726896075494 22.09402693060191 22 25.356772931292436 21.849464840603062 14.975831511105998 37.817930716998504 23 24.108067671768904 45.44539072390379 15.456324087927264 14.990217516400046 24 16.028887098630452 42.22004833697779 23.063643687420875 18.687420876970883 25 17.57188498402556 22.220303370463114 28.80580260771954 31.402009037791785 26 42.74198191973282 18.49772557148615 20.890194046179538 17.870098462601486 27 21.433920958635788 16.485194146791105 25.239082843645583 36.84180205092753 28 16.626339440027653 37.40350270768521 28.341398778661137 17.628759073625993 29 31.8764585554179 17.845515572329944 38.7277075110202 11.550318361231957 30 18.211691456243514 34.70252507782774 36.04289173296437 11.042891732964371 31 35.73858394833948 17.271102398523986 37.969903136531364 9.020410516605166 32 21.213519437074634 15.264159649325181 53.08282385511593 10.439497058484253 33 25.12765773302946 12.471511409860659 43.22476415774745 19.176066699362433 34 18.505266195354206 13.374693406434856 45.11614485644207 23.003895541768866 35 15.717625328292304 28.12490980980692 41.181563681491525 14.975901180409245 36 24.363342380319917 17.690708552289657 37.09938211006526 20.846566957325173 37 10.768341998844598 14.982668977469672 53.73194685153091 20.517042172154824 38 17.042302357836338 15.05143319463708 50.90730466944059 16.998959778085993 39 13.727359389895138 19.351763584366065 43.932172054193025 22.988704971545772 40 14.904749515797993 15.809556846760906 51.02477379816725 18.260919839273843 41 21.68256721595837 14.032957502168255 50.76322636600173 13.52124891587164 42 28.0138868797917 13.374801099377983 48.38998987415015 10.221322146680167 43 24.70128742948069 14.830030377549544 48.74005496889918 11.72862722407059 44 16.807476419188706 21.011515537295296 50.714657716567324 11.466350326948673 45 27.51953690303907 18.51808972503618 43.60057887120116 10.36179450072359 46 16.898717688945496 22.135062378788316 43.85619590702522 17.11002402524098 47 21.32576525440908 20.04286003880571 44.63235932929831 13.999015377486895 48 20.468107293899543 19.97277098661723 45.382654539134464 14.176467180348762 49 20.03068727925424 21.208963001563312 46.16119506687511 12.599154652307337 50 17.760203933837374 21.11178702818574 47.99107789461487 13.136931143362013 51 19.24335911474175 22.13724978998291 45.169606905941315 13.44978418933403 52 20.083451653096116 19.631421865492165 46.57355625742517 13.711570223986556 53 13.338162851347434 21.87771660388293 47.71660388293248 17.06751666183715 54 16.197958947002203 25.258030847732808 44.520468514438136 14.023541690826857 55 20.65557616508231 19.32819383259912 44.02967771852539 15.986552283793184 56 16.548326329517458 24.78481379510216 44.802202579336324 13.864657296044053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.5 14 19.0 15 29.5 16 40.0 17 40.0 18 40.0 19 79.5 20 119.0 21 270.5 22 544.0 23 666.0 24 604.5 25 543.0 26 571.5 27 600.0 28 755.0 29 1167.0 30 1424.0 31 1606.0 32 1788.0 33 1862.0 34 1936.0 35 1911.5 36 1887.0 37 1526.5 38 1936.5 39 2707.0 40 1731.0 41 755.0 42 614.5 43 474.0 44 425.5 45 377.0 46 396.0 47 685.5 48 956.0 49 1080.0 50 1204.0 51 973.5 52 743.0 53 702.5 54 949.0 55 1236.0 56 1908.5 57 2581.0 58 2211.0 59 1841.0 60 1598.5 61 1356.0 62 1354.5 63 1249.0 64 1145.0 65 1017.5 66 890.0 67 804.0 68 718.0 69 814.0 70 910.0 71 1001.5 72 1061.5 73 1030.0 74 757.5 75 485.0 76 324.0 77 163.0 78 113.0 79 42.0 80 21.0 81 14.0 82 7.0 83 6.0 84 5.0 85 4.0 86 3.0 87 1.5 88 0.0 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.037403613764529865 26 0.06329842329381978 27 0.11508804235239958 28 0.11508804235239958 29 0.13522844976406953 30 0.18414086776383934 31 0.1956496719990793 32 0.23017608470479917 33 0.26757969846932905 34 0.29059730693980895 35 0.3078605132926689 36 0.35101852917481874 37 0.3912993439981586 38 0.42582575670387846 39 0.3999309471745886 40 0.4689837725860283 41 0.47761537576245827 42 0.5495454022327081 43 0.5495454022327081 44 0.558177005409138 45 0.5927034181148579 46 0.6013350212912878 47 0.6473702382322477 48 0.6732650477615376 49 0.6157210265853378 50 0.6761422488203476 51 0.6761422488203476 52 0.7049142594084474 53 0.7077914604672575 54 0.7595810795258373 55 0.7250546668201174 56 0.7221774657613074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 34756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.22891011623892 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21741671537113 38.50558177005409 2 10.350672121566335 10.191046150304983 3 4.383401519579193 6.473702382322477 4 2.0338983050847457 4.005063873863506 5 1.4143775569842196 3.4814132811600875 6 0.7188778492109877 2.1233743814017725 7 0.5318527177089422 1.8327770744619634 8 0.4208065458796026 1.657267809874554 9 0.3331385154880187 1.4760041431695248 >10 1.4436002337814144 11.0599608700656 >50 0.07597895967270601 2.71607779951663 >100 0.058445353594389245 6.171596271147427 >500 0.005844535359438925 1.9306019104615029 >1k 0.01168907071887785 8.37553228219588 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1851 5.325699159857291 No Hit GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1060 3.049833122338589 No Hit GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 671 1.9306019104615029 No Hit GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 384 1.104845206583036 No Hit GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 298 0.8574059155253768 No Hit GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.7912302911727471 No Hit GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 236 0.6790194498791575 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 211 0.6070894234089078 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 201 0.578317412820808 No Hit GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 196 0.563931407526758 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT 137 0.39417654505696853 No Hit GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 0.30210611117504893 No Hit GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC 102 0.29347450799861896 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGG 100 0.28772010588099894 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGT 95 0.273334100586949 No Hit GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC 91 0.26182529635170904 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 83 0.23880768788122914 Illumina Single End Adapter 2 (95% over 23bp) GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC 75 0.21579007941074924 No Hit GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.20428127517550929 No Hit GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC 70 0.20140407411669928 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCC 67 0.1927724709402693 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCAGGCCGCCCC 63 0.18126366670502936 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCT 61 0.17550926458740937 No Hit GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG 59 0.16975486246978938 No Hit GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.15824605823454943 No Hit GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCCCC 54 0.15536885717573945 No Hit GGGCGAGGGGGGGTGTCCATGTATTGAAACTAAGCCCATAAACACGTTTGATTTGT 50 0.14386005294049947 No Hit GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.12947404764644954 No Hit GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG 45 0.12947404764644954 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 42 0.12084244447001956 Illumina Single End Adapter 2 (96% over 30bp) GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 41 0.11796524341120956 No Hit GGGCGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.11221084129358959 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT 39 0.11221084129358959 No Hit GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCCC 37 0.10645643917596961 No Hit GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTC 36 0.10357923811715962 No Hit GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA 35 0.10070203705834964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.04028081482333985 0.0 0.0 0.0 0.0 11 0.11508804235239958 0.0 0.0 0.0 0.0 12 0.11796524341120958 0.0 0.0 0.0 0.0 13 0.11796524341120958 0.0 0.0 0.0 0.0 14 0.1409828518816895 0.0 0.0 0.0 0.0 15 0.14673725399930948 0.0 0.0 0.0 0.0 16 0.14673725399930948 0.0 0.0 0.0 0.0 17 0.14673725399930948 0.0 0.0 0.0 0.0 18 0.14673725399930948 0.0 0.0 0.0 0.0 19 0.15536885717573945 0.0 0.0 0.0 0.0 20 0.1927724709402693 0.0 0.0 0.0 0.0 21 0.1927724709402693 0.0 0.0 0.0 0.0 22 0.20428127517550926 0.0 0.0 0.0 0.0 23 0.21866728046955922 0.0 0.0 0.0 0.0 24 0.23017608470479917 0.0 0.0 0.0 0.0 25 0.23017608470479917 0.0 0.0 0.0 0.0 26 0.23305328576360917 0.0 0.0 0.0 0.0 27 0.23305328576360917 0.0 0.0 0.0 0.0 28 0.3625273334100587 0.0 0.0 0.0 0.0 29 0.36540453446886867 0.0 0.0 0.0 0.0 30 0.42582575670387846 0.0 0.0 0.0 0.0 31 0.42870295776268846 0.0 0.0 0.0 0.0 32 0.43158015882149847 0.0 0.0 0.0 0.0 33 0.4344573598803084 0.0 0.0 0.0 0.0 34 0.44021176199792844 0.0 0.0 0.0 0.0 35 0.5121417884681781 0.0 0.0 0.0 0.0 36 0.5207733916446081 0.0 0.0 0.0 0.0 37 0.5265277937622281 0.0 0.0 0.0 0.0 38 0.532282195879848 0.0 0.0 0.0 0.0 39 0.540913799056278 0.0 0.0 0.0 0.0 40 0.5437910001150881 0.0 0.0 0.0 0.0 41 0.5437910001150881 0.0 0.0 0.0 0.0 42 0.549545402232708 0.0 0.0 0.0 0.0 43 0.561054206467948 0.0 0.0 0.0 0.0 44 0.569685809644378 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCGAA 35 4.9456503E-8 50.185238 50 GCCCCCC 25 2.0010752E-5 50.185238 50 TTTCCCC 15 0.008241063 50.185234 50 TGCGATC 35 5.0025847E-8 50.11272 32 GTGCGAT 35 5.0025847E-8 50.11272 31 CGTTTGA 15 0.008288404 50.112717 45 GTTTGCT 15 0.008288404 50.112717 32 GTGCAGA 20 4.08184E-4 50.112717 31 AGAAGTC 15 0.008288404 50.112717 41 CGCCCGG 15 0.008288404 50.112717 36 TCCTAAG 15 0.008288404 50.112717 46 CTGGGAG 15 0.008288404 50.112717 39 CCTAAGA 15 0.008288404 50.112717 47 GGCAAAC 15 0.008288404 50.112717 36 GTTCTAA 15 0.008288404 50.112717 35 AGAGGGA 15 0.008288404 50.112717 44 TAGTTCT 15 0.008288404 50.112717 33 TGGAGCG 15 0.008288404 50.112717 32 GAGTTTT 15 0.008288404 50.112717 34 AGCAGGC 25 2.0183283E-5 50.112717 44 >>END_MODULE