SRR212937_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212937_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31264
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1589	5.082523029682702	No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1051	3.361693961105425	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	706	2.258188331627431	No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	370	1.1834698055271238	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	295	0.9435772773797338	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	257	0.8220317297850563	No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	222	0.7100818833162743	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	219	0.7004861821903787	No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.671699078812692	No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	190	0.607727737973388	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	118	0.3774309109518936	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGG	106	0.3390481064483111	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	101	0.32305527123848515	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	95	0.303863868986694	No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.29426816786079835	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	91	0.29106960081883315	No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.26228249744114635	No Hit
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.22389969293756398	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCAGGCCGCCCC	69	0.2207011258955988	No Hit
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	66	0.21110542476970315	No Hit
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	62	0.19831115660184237	No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG	61	0.19511258955987718	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC	59	0.1887154554759468	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	55	0.17592118730808598	Illumina Single End Adapter 2 (95% over 23bp)
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCCCC	55	0.17592118730808598	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGT	53	0.16952405322415556	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCT	50	0.15992835209825998	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCC	49	0.15672978505629478	No Hit
GGGCGAGGGCTGGGCGAGGGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCG	46	0.14713408393039917	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	45	0.14393551688843398	Illumina Single End Adapter 2 (96% over 30bp)
GGGCGAGGGGGCTGGGCGAGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCG	44	0.14073694984646878	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCTCC	42	0.1343398157625384	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA	40	0.127942681678608	No Hit
GGGCGAGGGAAGAATGCACAAAGCAGAATCACATTCTTCCTTTCAAATTTCTCCTT	36	0.11514841351074719	No Hit
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.11514841351074719	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.11194984646878199	No Hit
GGGCGAGGGCAGTCCGCAGAGTCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.10555271238485157	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	32	0.1023541453428864	Illumina Single End Adapter 2 (100% over 29bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACAAG	15	0.008269122	50.130226	46
ATCCTAC	15	0.008269122	50.130226	43
CGGAGCA	25	2.0093925E-5	50.130226	41
CCTACAA	15	0.008269122	50.130226	45
TCCTACA	15	0.008269122	50.130226	44
GGCGCGG	25	2.0093925E-5	50.130226	42
CCCGGCA	15	0.008269122	50.130226	37
CATCCTA	15	0.008269122	50.130226	42
CGCCACC	25	2.0093925E-5	50.130226	35
CATTTGG	15	0.008269122	50.130226	45
GTGGGGG	15	0.008269122	50.130226	36
TACAAGG	15	0.008269122	50.130226	47
TCGCCAC	25	2.0093925E-5	50.130226	34
TCAGAGG	15	0.008269122	50.130226	48
CGGTGGG	15	0.008269122	50.130226	34
CCTTTGG	15	0.008269122	50.130226	45
GATCATC	15	0.008269122	50.130226	39
GGTAGGC	15	0.008269122	50.130226	48
ATTTGGT	15	0.008269122	50.130226	46
ATCATCC	15	0.008269122	50.130226	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content